Journal of Experimental Biology and Agricultural Sciences最新文献_第2页

AN ASSESSMENT OF PESTICIDE POISONING INCIDENCES PRESENTED AT HEALTH CARE FACILITIES IN MASHONALAND CENTRAL PROVINCE, ZIMBABWE 对津巴布韦马绍纳兰中央省医疗机构发生的杀虫剂中毒事件的评估

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2024-05-15 DOI: 10.18006/2024.12(2).226.236

Kenneth Chipere, Claid Mujaju, Taurai Matyora, Shumirayi Muhera, Shengai Mwale, Peter Ishe Muzofa, Kudzai Kusena, Ivy Getrude Saunyama

{"title":"AN ASSESSMENT OF PESTICIDE POISONING INCIDENCES PRESENTED AT HEALTH CARE FACILITIES IN MASHONALAND CENTRAL PROVINCE, ZIMBABWE","authors":"Kenneth Chipere, Claid Mujaju, Taurai Matyora, Shumirayi Muhera, Shengai Mwale, Peter Ishe Muzofa, Kudzai Kusena, Ivy Getrude Saunyama","doi":"10.18006/2024.12(2).226.236","DOIUrl":"https://doi.org/10.18006/2024.12(2).226.236","url":null,"abstract":"Acute Pesticide Poisoning (APP) is a significant problem in developing countries, causing loss of productivity and fatalities in the agricultural sector. However, limited information on the connection between pesticide use, APP, and deaths in Zimbabwe is available. This study aimed to evaluate incidents that resulted in APP and identify the specific pesticides that caused them at Health Care Facilities (HCFs) in Mashonaland Central Province. Researchers conducted a survey asking standardized questions to gather information on APP cases from 93 HCFs. Descriptive statistics and chi-square association tests for APP cases in the targeted HCFs were calculated using IBM SPSS version 22. The study found that 43% of APP cases were due to pesticides belonging to the World Health Organization (WHO) class II acute toxicity category, while 26.1% were caused by Highly Hazardous Pesticides (HHPs). Most of the cases were due to intentional poisoning (87.1%), with the majority (55.9%) of APP incidents being males. The highest APP cases were recorded in the 21-30 age group (38.8%), followed by the 31-40 age group (32.8%). These results emphasize the significant impact of intentional poisoning by WHO class II type pesticides, particularly HHPs, on the incidence of APP in Zimbabwe. To mitigate the impact of HHPs on human health, it is recommended that the government of Zimbabwe consider pesticide risk reduction measures, such as stricter pesticide registration criteria, import restrictions, and the promotion of less toxic alternatives. These findings highlight the urgent need for policymakers, researchers, and other stakeholders in the agricultural sector to work collaboratively towards creating a safer and more sustainable farming environment in Zimbabwe.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":" 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141127836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethnopharmacological study of medicinal plants used in the treatment of skin diseases in the Western Middle Atlas region (Morocco) 对中阿特拉斯西部地区（摩洛哥）用于治疗皮肤病的药用植物的民族药理学研究

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2024-03-15 DOI: 10.18006/2024.12(1).93.105

Fatiha El Azzouzi, Soukaina Chaouqi, Meryem Makkaoui, H. Briguiche, L. Zidane

{"title":"Ethnopharmacological study of medicinal plants used in the treatment of skin diseases in the Western Middle Atlas region (Morocco)","authors":"Fatiha El Azzouzi, Soukaina Chaouqi, Meryem Makkaoui, H. Briguiche, L. Zidane","doi":"10.18006/2024.12(1).93.105","DOIUrl":"https://doi.org/10.18006/2024.12(1).93.105","url":null,"abstract":"An investigation was conducted among 360 people from the local population of the Western Middle Atlas of Morocco to identify medicinal plants used for treating skin diseases. Various parameters, including Relative Frequency of Citation (RFC), Family Use Value (FUV), Plant Part Value (PPV), Informant Consensus Factor (ICF), and Fidelity Level (FL), were used for data collection and assessment. During the investigation, 45 medicinal plant species belonging to 33 families were documented, with the most important family being Euphorbiaceae (FUV = 0.292). The highest ICF value (ICF=0.991) was mentioned for skin cancer. The poultice was found to be the primary method for preparing the majority of remedies (51%). Leaves were the most commonly used plant part (PPV = 0.476), and Allium sativum L. was the most widely used species (RFC = 0.302). These findings are a preliminary step towards conserving and popularising these plant species, promoting sustainable practices in traditional medicine, safeguarding biodiversity, and integrating these valuable botanical resources into modern healthcare systems.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"12 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular regulation of Mycobacterium tuberculosis Sigma factor H with Anti-sigma factor RshA under stress condition 压力条件下结核分枝杆菌σ因子 H 与反σ因子 RshA 的分子调控

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2024-03-15 DOI: 10.18006/2024.12(1).153.162

Sayantee Sen, Swarnav Bhakta, Kuntal Pal

{"title":"Molecular regulation of Mycobacterium tuberculosis Sigma factor H with Anti-sigma factor RshA under stress condition","authors":"Sayantee Sen, Swarnav Bhakta, Kuntal Pal","doi":"10.18006/2024.12(1).153.162","DOIUrl":"https://doi.org/10.18006/2024.12(1).153.162","url":null,"abstract":"Mycobacterium tuberculosis is the causative agent of tuberculosis, the leading fatal infectious disease that claims millions of lives every year. M. tuberculosis regulates its stress condition response using its regulatory protein, Sigma Factor H, which binds with its cognate anti-sigma factor RshA in normal conditions, forming a complex inhibiting transcription. During oxidative stress, SigH is released from the complex and binds to RNA Polymerase (RNAP) to initiate transcription. Thus, it is important to understand the molecular conformational state of SigH in complex with different protein partners under different cellular or environmental contexts. This work intends to analyze the SigH-RshA complex, which revealed the variation in SigH shown during complex formation with RNAP and RshA, respectively. Previously, Hydrogen Deuterium Exchange-Mass Spectrometry (HDX-MS) analysis of SigH-RshA interaction provided a detailed insight into the critical residues participating in the interaction. The HDX-MS data were used to dock RshA on the open conformation of SigH from the SigH-RNAP complex structure (PDB: 5ZX2), and closed conformation was obtained from protein modelling. The docking revealed that closed conformation of SigH complexing with RshA in terms of HDX-MS data revealed a major structural shift in SigH while interacting with two different binding partners, RshA and RNAP, under variable environmental conditions. This structural shift of SigH with RshA and RNAP has significance in understanding the stress response of M. tuberculosis, and SigH could prove to be a potential drug target.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"24 94","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140236898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highlighting the Importance of Matrix Metalloproteinase 1, 8, and 9 Expression during the Progression of Mycobacterium tuberculosis Infection 突显基质金属蛋白酶 1、8 和 9 表达在结核分枝杆菌感染进展过程中的重要性

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2024-03-15 DOI: 10.18006/2024.12(1).49.59

Sasikumar Pitchaikani, Murugan Mukilan, Pothiaraj Govindan, Ganesan Kathiravan, Harshavardhan Shakila

{"title":"Highlighting the Importance of Matrix Metalloproteinase 1, 8, and 9 Expression during the Progression of Mycobacterium tuberculosis Infection","authors":"Sasikumar Pitchaikani, Murugan Mukilan, Pothiaraj Govindan, Ganesan Kathiravan, Harshavardhan Shakila","doi":"10.18006/2024.12(1).49.59","DOIUrl":"https://doi.org/10.18006/2024.12(1).49.59","url":null,"abstract":"Tuberculosis (TB) is one of the major threats to public health; annually it kills more than 1.5 million people around the globe. Tuberculosis is caused by an intracellular pathogen named Mycobacterium tuberculosis (Mtb). This Mtb enters the lung through the respiratory passage by inhalation in healthy individuals. Infection of this disease starts from the settlement of Mtb to the lung alveoli of the host from the external bacilli air droplets. After settlement, the multiplication of Mtb results in the induction of innate immunity through the alveolar macrophages. Compared to other infectious diseases, tuberculosis infection was transmitted rapidly by the infected aerosols released from infected persons to healthy persons through the air. After infection, disease development results in the formation of drug-resistance TB (DR-TB) with four subcategories, i.e. Single-drug resistant TB (SDR-TB), multi-drug resistant TB (MDR-TB), extensive drug-resistant TB (XDR-TB), and total-drug resistant TB (TDR-TB). As a result, this DR-TB may act as a major source of TB death due to spontaneous antimicrobial resistance (AMR). This AMR makes the anti-TB drugs ineffective. In the current scenario, researchers are trying to find the drug target to decrease tuberculosis progression instead of drug resistance. The present review reports that the outcome of research studies showed that matrix metalloproteinase (MMP) may act as a suitable target for treating Mtb infection with the help of specific proteinase inhibitors. Recent reports have shown the specific role of matrix metalloproteinases 1, 8, and 9 in the disease progression and its role in normal homeostasis mechanism with the help of specific animal models/In vitro models.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"15 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140240883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytotoxicity and genotoxicity assessment of organic and inorganic contaminants detected in pharmaceutical industrial wastewaters using Vigna radiata and Allium cepa 利用金莲花和牛肝菌对制药工业废水中检测到的有机和无机污染物进行植物毒性和遗传毒性评估

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2024-03-15 DOI: 10.18006/2024.12(1).76.92

Km Jyoti, K. Soni, Ram Chandra

{"title":"Phytotoxicity and genotoxicity assessment of organic and inorganic contaminants detected in pharmaceutical industrial wastewaters using Vigna radiata and Allium cepa","authors":"Km Jyoti, K. Soni, Ram Chandra","doi":"10.18006/2024.12(1).76.92","DOIUrl":"https://doi.org/10.18006/2024.12(1).76.92","url":null,"abstract":"The discharged effluent of pharmaceutical industrial wastewater treatment plants (PIWWTPs) exhibits substantial environmental toxicity due to the intricate combination of organic and inorganic pollutants. This study assessed the phytotoxicity, genotoxicity, and cytotoxicity of untreated and treated pharmaceutical industrial wastewater (PIWW). Most of the physicochemical parameters viz. COD, BOD, EC, sulfide, sulfate, nitrate, phosphate, grease, phenols, and metal concentrations viz. B, Cr, Ca, Cd, Cu, Zn, Pb, Hg, and As in untreated wastewater (UTW) were noted beyond the permissible limit and remained higher in treated wastewater (TW). The findings revealed that the performance of PIWWTP was woefully inadequate. The GC-MS spectra of UTW and TW revealed the presence of various organic contaminants. The toxicological studies showed that the UTW had a high degree of phytotoxicity, which persisted even after the treatment as it inhibited the seed germination in Vigna radiata. The seed germination was inhibited up to 70% and 50% tested at 50% concentration of UTW and TW respectively. Genotoxicity was measured by determining mitotic index and chromosomal aberrations in Allium cepa root apex grown in untreated and treated PIWW. Compared to the negative control, the mitotic index dropped to 85% and 75% at the 50% concentrations of UTW and TW, respectively. Chromosomal aberrations were also found in the cellular mass of root apex growing in both UTW and TW. According to the findings, it is unsafe for the environment to release PIWW that has not been properly treated, as this could pose serious risks to environmental health.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"125 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140237671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Probiotic Strain Lactobacillus acidophilus in the Reversal of Gut Dysbiosis Induced Brain Cognitive Decline 益生菌株嗜酸乳杆菌在逆转肠道菌群失调引起的大脑认知能力下降中的作用

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2024-03-15 DOI: 10.18006/2024.12(1).36.48

Murugan Mukilan, Mepully Thomas Antony Mathew, Siva Yaswanth, Vivekanandan Mallikarjun

{"title":"Role of Probiotic Strain Lactobacillus acidophilus in the Reversal of Gut Dysbiosis Induced Brain Cognitive Decline","authors":"Murugan Mukilan, Mepully Thomas Antony Mathew, Siva Yaswanth, Vivekanandan Mallikarjun","doi":"10.18006/2024.12(1).36.48","DOIUrl":"https://doi.org/10.18006/2024.12(1).36.48","url":null,"abstract":"In the central nervous system, bidirectional communication between the brain and gut results in memory formation due to synaptic plasticity changes. During a healthy state, oral balanced microflora plays a pivotal role in memory formation by inhibiting the enterotoxin level produced by infectious pathogens. In disease conditions, beneficial microbial dysbiosis may result in excess enterotoxin production. Further, excess enterotoxin secretion prevents beneficial bacteria's proliferation and impairs neurotransmitter precursor compounds' transport to the brain. Blockade of neurotransmitter precursor compounds may result in the development of memory loss. The present study stated the role of Lactobacillus acidophilus in recovering memory loss. Reversal of cognitive impairment is shown with the help of a three-step behavioural analysis, which consists of one pre-infusive behavioural analysis and two post-infusive behavioural analyses (phase 1 and 2). The pre-infusive analysis showed no cognitive impairment in an assimilated environment without any infusions. After oral microbial infusions, phase 1 of post-infusive behavioural analysis showed the presence of cognitive impairment in the experimental groups who received oral infusions. Formed cognitive impairment is reverted with the help of L. acidophilus oral infusion in phase 2 of post-infusive analysis. Comparative three-step behavioural analysis proved that Pseudomonas aeuroginosa induced cognitive impairment may revert to normal conditions with the help of L. acidophilus. The outcome of the present study proves that cognitive impairment developed due to poor oral hygiene can be treated with the help of probiotic microorganisms.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"107 S8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140238005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration and Profiling of Potential Thermo-alkaliphilic Bacillus licheniformis and Burkholderia sp. from varied Soil of Delhi region, India and their Plant Growth-Promoting Traits 探索和分析印度德里地区不同土壤中潜在的地衣芽孢杆菌和伯克霍尔德氏菌及其促进植物生长的特性

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2024-03-15 DOI: 10.18006/2024.12(1).60.75

Charu Singh, A. Chauhan, Jayati Arora, Anuj Ranjan, Hardeep Singh Tuli, M. Shahwan, Vishnu D. Rajput, T. Minkina, Sambasivan Venkat Eswaran, T. Jindal

{"title":"Exploration and Profiling of Potential Thermo-alkaliphilic Bacillus licheniformis and Burkholderia sp. from varied Soil of Delhi region, India and their Plant Growth-Promoting Traits","authors":"Charu Singh, A. Chauhan, Jayati Arora, Anuj Ranjan, Hardeep Singh Tuli, M. Shahwan, Vishnu D. Rajput, T. Minkina, Sambasivan Venkat Eswaran, T. Jindal","doi":"10.18006/2024.12(1).60.75","DOIUrl":"https://doi.org/10.18006/2024.12(1).60.75","url":null,"abstract":"Soilless cultivation has emerged as a fundamental alternative for large-scale vegetable production because it generates high-quality yields and uses resources efficiently. While plant growth-promoting bacteria (PGPB) are known to enhance growth and physiological aspects in crops grown in soil, their application in soilless cultivation has been relatively less explored. This study aimed to isolate potential PGPBs from soil samples collected from five locations in and around the Delhi-National Capital Region (NCR), India, which were further screened for significant PGPB attributes. Among these, 51 isolated were selected for assessing the impact on Oryza sativa (rice) growth and yield grown on a hydroponic set. The results indicated that isolates AFSI16 and ACSI02 significantly improved the physiological parameters of the plants. For instance, treatment with AFSI16 showed a 23.27% increase in maximum fresh shoot mass, while ACSI02 resulted in a 46.8% increase in root fresh mass. Additionally, ACSI02 exhibited the highest shoot length (34.07%), whereas AFSI16 exhibited the longest root length (46.08%) in O.sativa. Treatment with AFSI16 also led to significant increases in total protein content (4.94%) and chlorophyll content (23.44%), while ACSI02 treatment showed a 13.48% increase in maximum carotenoid content in the leaves. The potential PGPBs were identified through 16S rRNA sequencing, as the two most effective strains, AFSI16 and ACSI02, belonged to thermo-alkaliphilic Bacillus licheniformis and Burkholderia sp., respectively. This study demonstrated the potential of these identified PGPB strains in enhancing crop performance, specifically in soilless cultivation systems.\u0000 ","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"34 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140238885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyzing the antimicrobial efficacy of the economically important tree Knema linifolia (Roxb.) Warb 分析具有重要经济价值的树种 Knema linifolia (Roxb.) Warb 的抗菌功效

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2024-03-15 DOI: 10.18006/2024.12(1).145.152

Ria Bhar, Arpita Das, Srijan Haldar, Joydeep Paul

{"title":"Analyzing the antimicrobial efficacy of the economically important tree Knema linifolia (Roxb.) Warb","authors":"Ria Bhar, Arpita Das, Srijan Haldar, Joydeep Paul","doi":"10.18006/2024.12(1).145.152","DOIUrl":"https://doi.org/10.18006/2024.12(1).145.152","url":null,"abstract":"Knema linifolia is widely used for fuel wood, fodder and healthcare purposes. This plant treats various diseases in different parts of India, including Assam, Meghalaya, Alipurduar and Darjeeling districts of West Bengal. This study was carried out to determine the bactericidal properties of various parts of K. linifolia aqueous extract. The aqueous extract of the leaves, bark, stem and plant sap were tested against Escherichia coli (gram-negative bacteria) & Staphylococcus aureus (gram-positive bacteria). Among the tested extracts, both the leaf and bark extracts were found to have high bactericidal potential and can kill more than 60% of both bacterial strains with a concentration of 300µg/mL through an agar diffusion test. The MIC (Minimum Inhibitory Concentration) values for the leaf and bark extracts were recorded at ≤1000µg/mL & ≤500µg/mL, respectively. It has also been found that both the bark and leaf extracts contain high tannins, which might be essential for the antibacterial properties of Knema sp. There is currently a lack of proper documentation on using K. linifolia, which makes it challenging to conduct clinical or commercial research to support new uses in modern phototherapy. This study aims to fill this gap and provide significant information that could lead to changes in modern medicine.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"50 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140238701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Residue-specific orientation of arrestin in 5-HTR1B (Serotonin Receptor)- βArrestin-1 interaction 5-HTR1B（5-羟色胺受体）- β Arrestin-1 相互作用中 arrestin 的特定残基取向

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2024-03-15 DOI: 10.18006/2024.12(1).138.144

Somdatta Bhattacharya, Joydeep Paul, Srijan Haldar, Kuntal Pal

{"title":"Residue-specific orientation of arrestin in 5-HTR1B (Serotonin Receptor)- βArrestin-1 interaction","authors":"Somdatta Bhattacharya, Joydeep Paul, Srijan Haldar, Kuntal Pal","doi":"10.18006/2024.12(1).138.144","DOIUrl":"https://doi.org/10.18006/2024.12(1).138.144","url":null,"abstract":"Physiologically G protein-coupled receptors (GPCRs) are an important class of cell surface proteins capable of sensing the exogenous signals across the cell membrane through G-protein-dependent and independent pathways. Activated GPCRs initiate diverse G-protein-independent signalling through interaction with arrestin. Arrestins comprise a family of four proteins that act as signal regulators of GPCRs. Arrestin specificity and assembly orientation with a particular GPCR depend on the finger loop's residues. Recent cryo-EM structural elucidation of neurotensin receptor-1(NTSR1)-β-arrestin1complex reveals its striking difference from Rhodopsin-visual-Arrestin by a 90˚ rotation of β-Arrestin1 concerning the receptor. Alignment of neurotensin receptor 1(NTSR1)-β-Arrestin1 assembly with 5-HTR1B (Serotonin receptor) structure shows an ionic interaction mediated complex formation between receptor binding cleft and finger loop of arrestin. Mutational analysis of finger loop residues R65, D67, and D69 of β-Arrestin1 by tango assay confirms its possible interaction with an electropositive pocket of K79 and R161 in 5-HTR1B.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"17 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140240605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FIRST REPORT ON TRUFFLE-INHABITING FUNGI AND METAGENOMIC COMMUNITIES OF TUBER AESTIVUM COLLECTED IN RUSSIA 首次报告俄罗斯采集的块茎中的松露栖息真菌和元基因组群落

Journal of Experimental Biology and Agricultural Sciences Pub Date : 2024-03-15 DOI: 10.18006/2024.12(1).16.35

E. Malygina, N. A. Imidoeva, M. Morgunova, M. E. Dmitrieva, A. Belyshenko, A. A. Vlasova, V. N. Shelkovnikova, T.Yu. Telnova, A. S. Konovalov, D. Axenov-Gribanov

{"title":"FIRST REPORT ON TRUFFLE-INHABITING FUNGI AND METAGENOMIC COMMUNITIES OF TUBER AESTIVUM COLLECTED IN RUSSIA","authors":"E. Malygina, N. A. Imidoeva, M. Morgunova, M. E. Dmitrieva, A. Belyshenko, A. A. Vlasova, V. N. Shelkovnikova, T.Yu. Telnova, A. S. Konovalov, D. Axenov-Gribanov","doi":"10.18006/2024.12(1).16.35","DOIUrl":"https://doi.org/10.18006/2024.12(1).16.35","url":null,"abstract":"Truffles are one of the least studied groups of fungi in terms of their biological and biotechnological aspects. This study aimed to isolate truffle-inhabiting fungi and assess the metagenomic communities of the most common Russian summer truffle, Tuber aestivum. This study is the first to characterize the biodiversity of prokaryotic and eukaryotic organisms living in the truffle T. aestivum using molecular analysis and sequencing. Plant pathogens involved in a symbiotic relationship with truffles were identified by sequencing the hypervariable fragments of the 16S rRNA and 18S rRNA genes. In addition, some strains of fungal symbionts and likely pathogens were isolated and recognized for the first time from the truffles. This study also compared and characterized the general diversity and distribution of microbial taxa of T. aestivum collected in Russia and Europe. The results revealed that the Russian and European truffle study materials demonstrated high similarity. In addition to the truffles, representatives of bacteria, fungi, and protists were found in the fruiting bodies. Many of these prokaryotic and eukaryotic species inhabiting truffles might influence them, help them form mycorrhizae with trees, and regulate biological processes. Thus, truffles are interesting and promising sources for modern biotechnological and agricultural studies.","PeriodicalId":15766,"journal":{"name":"Journal of Experimental Biology and Agricultural Sciences","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140237616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0