E. Malygina, N. A. Imidoeva, M. Morgunova, M. E. Dmitrieva, A. Belyshenko, A. A. Vlasova, V. N. Shelkovnikova, T.Yu. Telnova, A. S. Konovalov, D. Axenov-Gribanov
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引用次数: 0
摘要
松露是在生物和生物技术方面研究最少的真菌之一。本研究旨在分离松露栖息真菌,并评估俄罗斯最常见的夏季松露--块菌的元基因组群落。这项研究首次利用分子分析和测序技术描述了生活在块菌 T. aestivum 中的原核生物和真核生物的生物多样性特征。通过对 16S rRNA 和 18S rRNA 基因的超变异片段进行测序,确定了与松露有共生关系的植物病原体。此外,还首次从松露中分离并确认了一些真菌共生菌株和可能的病原体。这项研究还比较并描述了在俄罗斯和欧洲采集的松露微生物类群的总体多样性和分布情况。研究结果表明,俄罗斯和欧洲的松露研究材料具有很高的相似性。除了松露之外,还在子实体中发现了细菌、真菌和原生生物的代表。松露中栖息的许多原核生物和真核生物可能会影响松露,帮助松露与树木形成菌根,并调节生物过程。因此,松露是现代生物技术和农业研究的有趣而有前途的资源。
FIRST REPORT ON TRUFFLE-INHABITING FUNGI AND METAGENOMIC COMMUNITIES OF TUBER AESTIVUM COLLECTED IN RUSSIA
Truffles are one of the least studied groups of fungi in terms of their biological and biotechnological aspects. This study aimed to isolate truffle-inhabiting fungi and assess the metagenomic communities of the most common Russian summer truffle, Tuber aestivum. This study is the first to characterize the biodiversity of prokaryotic and eukaryotic organisms living in the truffle T. aestivum using molecular analysis and sequencing. Plant pathogens involved in a symbiotic relationship with truffles were identified by sequencing the hypervariable fragments of the 16S rRNA and 18S rRNA genes. In addition, some strains of fungal symbionts and likely pathogens were isolated and recognized for the first time from the truffles. This study also compared and characterized the general diversity and distribution of microbial taxa of T. aestivum collected in Russia and Europe. The results revealed that the Russian and European truffle study materials demonstrated high similarity. In addition to the truffles, representatives of bacteria, fungi, and protists were found in the fruiting bodies. Many of these prokaryotic and eukaryotic species inhabiting truffles might influence them, help them form mycorrhizae with trees, and regulate biological processes. Thus, truffles are interesting and promising sources for modern biotechnological and agricultural studies.