S. V. Joseph, Angelita L. Acebes-Doria, Brett Blaauw
{"title":"A Survey on Ambrosia Beetle (Coleoptera: Curculionidae) Problems in Ornamental and Pecan Industries in Georgia1","authors":"S. V. Joseph, Angelita L. Acebes-Doria, Brett Blaauw","doi":"10.18474/jes23-06","DOIUrl":"https://doi.org/10.18474/jes23-06","url":null,"abstract":"\u0000 The ambrosia beetles, mainly Xylosandrus crassiusculus (Motschulsky) (Coleoptera: Curculionidae: Scolytinae), are serious pests of young trees in the ornamental industry and pecan orchards as infested trees develop branch dieback with occasional tree mortality. Surveys were conducted in the ornamental industry in 2020 and 2021 and the pecan industry in 2020 because clientele perspective on the extent of damage, phenology, monitoring, management, and loss related to the ambrosia beetle was unclear. Of 35 and 40 ornamental industry respondents, 68% and 82% indicated problems with this pest in 2020 and 2021, respectively. Of 66 pecan industry respondents, 52% reported beetle problems in 2020. About 85% of ornamental and 58% of pecan respondents indicated that 1–10 trees were attacked by ambrosia beetles and 1–30 or more trees were culled annually. The beetle problem persists throughout the growing season but appears greater during the spring than in other periods. About 73% of respondents indicated that current monitoring tools helped them with management decisions; however, a proportion did not use recommended monitoring tools but instead relied on visual signs to determine attacks on trees. In the 2020 surveys, only 37% of ornamental respondents and 43% of the pecan respondents used insecticide sprays, whereas in the 2021 survey, 71% of the ornamental clientele (mostly nurseries) sprayed pyrethroid insecticides for ambrosia beetle management. In 2020 surveys, about 48% and 56% of ornamental and pecan respondents, respectively, spent <$500 USD for ambrosia beetle management.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44582249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gunalan Chandrasekaran, Jeyarani Subramanian, M. Marimuthu, Mohankumar Subbarayalu, Haripriya Shanmugam
{"title":"Invasive Whitefly (Hemiptera: Aleyrodidae) Complex and Diversity in Coconut Landscapes in Tamil Nadu1","authors":"Gunalan Chandrasekaran, Jeyarani Subramanian, M. Marimuthu, Mohankumar Subbarayalu, Haripriya Shanmugam","doi":"10.18474/jes23-17","DOIUrl":"https://doi.org/10.18474/jes23-17","url":null,"abstract":"\u0000 Coconut, Cocos nucifera L., in Tamil Nadu, India, was invaded by four whitefly (Hemiptera: Aleyrodidae) species between 2016 and 2019, increasing the total to six, along with Aleurodicus dispersus Russell and Aleurocanthus arecae David and Manjunatha. We examined the complexity and diversity of whiteflies in coconut across different agroclimatic zones of Tamil Nadu and found high and low damage levels by whiteflies at Veppankulam (70.4%) and Rameswaram (11.0%), respectively. The rugose spiraling whitefly, Aleurodicus rugioperculatus Martin, was the most dominant species but was effectively managed by the parasitoids Encarsia guadeloupae Viggiani and E. dispersa Polaszek, with the highest rate parasitism of 83.8% at Aliyar Nagar and the lowest (2.9%) in the Kanchipuram district. Aleurodicus rugioperculatus and Bondar’s nesting whitefly, Paraleyrodes bondari Peracchi, have spread throughout Tamil Nadu. The population of A. rugioperculaus was high at Veppankulam and that of P. bondari was higher at Dharmapuri (28.6 and 31.1 adults/leaflet, respectively). All five invasive whitefly species in coconut were found only in 7 of 34 locations—Coimbatore, Sirumugai, Salem, Dharmapuri, Krishnagiri, Vellore, and Erode. Paraleyrodes minei Iaccarino and Aleurotrachelus atratus Hempel were distributed in the northwestern zone adjoining the western ghats of Tamil Nadu. Coconut plantations at Sirumugai (western ghats) supported the most diverse collection of whiteflies, with 0.61 and 1.09 Simpson and Shannon diversity indices. Among the 29 districts, adjoining Dindigul and Theni districts showed the highest Bray–Curtis Similarity Matrix. Butterfly palm, Dypsis lutescens H. Wendel, custard apple, Annona squamosa L., and guava, Psidium guajava L., are whitefly hosts in coconut landscapes.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44577873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bioactivity of Artemisia vulgaris Essential Oil and Two of Its Constituents Against the Red Flour Beetle (Coleoptera: Tenebrionidae)","authors":"Shanshan Gao, Mengyi Guo, Yang Yin, Xinyi Zhang, Yong-lei Zhang, Kunpeng Zhang","doi":"10.18474/JES22-49","DOIUrl":"https://doi.org/10.18474/JES22-49","url":null,"abstract":"Abstract Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) is one of the most destructive pests of stored products. Although there have been studies on the potential use of essential oils from plants in the Artemisia genus as insecticides, no comprehensive bioactivity data are available on the efficacy of Artemisia vulgaris L. (Asterales: Asteraceae) essential oil and its chemical constituents on stored-product pests. Therefore, in this study, the bioactivity of A. vulgaris essential oil and its chemical constituents, eugenol and terpinen-4-ol, against T. castaneum were determined by contact, fumigant, and repellent bioassays. Analysis of contact and fumigant bioassays showed that A. vulgaris essential oil, eugenol, and terpinen-4-ol have contact and fumigant toxicities against T. castaneum, of which terpinen-4-ol has a strong killing effect on larvae and adults, suggesting that terpinen-4-ol may be the main active component of A. vulgaris essential oil in contact and fumigant effects. Additionally, A. vulgaris essential oil and eugenol have higher repellent activity against T. castaneum larvae and adults, whereas the repellent activity of terpinen-4-ol is low, indicating that the main component of A. vulgaris essential oil in repellence may be eugenol. These results further provide relevant theoretical basis for the development of plant essential oil pesticides.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":"58 1","pages":"355 - 369"},"PeriodicalIF":0.9,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44215466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Systemic Insecticides in Protecting Container-Grown Nursery Plants from Damage Caused by Field-Collected Populations of Redheaded Flea Beetle, Systena frontalis (Coleoptera: Chrysomelidae), Adults","authors":"R. Cloyd, N. J. Herrick","doi":"10.18474/JES22-69","DOIUrl":"https://doi.org/10.18474/JES22-69","url":null,"abstract":"Abstract Systena frontalis (F.) is a major insect pest of nursery production systems in the Midwest, Northeast, and Southeast regions of the United States. Adults feed on plant leaves, which reduces salability of nursery-grown plants. There are limited options available to protect plants from S. frontalis adult feeding damage, and foliar insecticide applications are labor intensive. Systemic insecticides applied to the growing medium may protect plants from S. frontalis adult feeding. Consequently, in 2021 and 2022, we conducted two laboratory and two greenhouse experiments to determine if the systemic insecticides thiamethoxam, dinotefuran, cyantraniliprole, acephate, imidacloprid, and cyfluthrin + imidalcoprid protect plants from feeding by field-collected populations of S. frontalis adults. In the laboratory experiments, growing medium containing Itea plants was treated with the aforementioned systemic insecticides. Seven and 25 d after the treatments were applied, leaves were collected and placed into Petri dishes with a single S. frontalis adult. In the greenhouse experiments, Itea plants were placed into observation cages. Eight S. frontalis adults were released into each cage with an Itea plant. In the laboratory experiments, treatments associated with thiamethoxam and dinotefuran resulted in 100% mortality of S. frontalis adults after 72 h. In the greenhouse experiments, thiamethoxam, dinotefuran, and acephate protected plants from S. frontalis adult feeding 7 d after applying the systemic insecticide treatments. Therefore, systemic insecticides can mitigate feeding damage caused by S. frontalis adults on nursery-grown plants.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":"58 1","pages":"294 - 306"},"PeriodicalIF":0.9,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45588980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preparation and Application Analysis of a Polyclonal Antibody as Reference Protein in Helicoverpa armigera (Lepidoptera: Noctuidae)","authors":"Yun Tan, Ruoheng Jin, Zhengkun Xiao, Guo‐Hua Huang","doi":"10.18474/JES22-71","DOIUrl":"https://doi.org/10.18474/JES22-71","url":null,"abstract":"Abstract A stable and specific heat shock protein 27.2 (HSP27.2) antibody was prepared and analyzed for protein level research in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae). The full-length hsp27.2 was amplified from H. armigera larvae and constructed into the prokaryotic expression vector. The purified His-tag fused protein was used to immunize rabbits for the antibody preparation. Western blot analysis indicated that this antibody specifically recognized the HSP27.2 encoded by H. armigera and detected the HSP27.2 encoded by other noctuid larvae. Further analysis of HSP27.2 expression in H. armigera under infection by different pathogenic microorganisms and in different tissues showed that the expression of HSP27.2 is continually stable. The HSP27.2 antibody is efficient and capable as a reference antibody for functional studies involving genes and proteins in H. armigera and other lepidopteran insects.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":"58 1","pages":"344 - 354"},"PeriodicalIF":0.9,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42445256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Tong, M. Gullickson, Mary Rogers, E. Burkness, W. Hutchison
{"title":"Detection of Spotted-winged Drosophila (Diptera: Drosophilidae) Infestations in Blueberry Fruits1","authors":"C. Tong, M. Gullickson, Mary Rogers, E. Burkness, W. Hutchison","doi":"10.18474/JES22-70","DOIUrl":"https://doi.org/10.18474/JES22-70","url":null,"abstract":"Spotted-winged drosophila, Drosophila suzukii Matsumura (Diptera: Drosophilidae), has become a significant pest of small and stone fruit. Unlike most Drosophila species, it tends to infest healthy, intact ripe fruit, as opposed to rotting or overripe fruit (Mitsui et al. 2006, Popul. Ecol. 48:233–237; Asplen et al. 2015, J. Pest Sci. 88:469–494). Spotted-winged drosophila adults are typically detected in the field using baited traps. This is useful in helping growers decide when to apply insecticides (Ebbenga et al. 2022, J. Entomol. Sci. 57: 516–529), but methods are also needed to estimate actual fruit infestation levels. Spectral imaging of fruit may provide a nondestructive alternative to extraction of larvae and could provide information on the infestation status of a single fruit. Such imaging has been tested for insect pests other than D. suzukii. For example, Peshlov et al. (2009, J. Near Infrared Spectrosc. 17:203–212) used near-infrared spectroscopy (NIRS) to detect infestation of wild blueberries (Vaccinium) by blueberry maggot, Rhagoletis mendax Curran (Diptera: Tephritidae). By measuring spectra of a live larva and subtracting it from an infested blueberry, they demonstrated that the NIR signal they recorded was from a larva and ‘‘associated chemical changes in the blueberries.’’ Detectable differences between infested blueberry and larvae occurred between approximately 750 and 1300 nm, with a small differential signal at 600 nm. Tsuta et al. (2006, Food Sci. Technol. Res. 12:96–100) also used spectroscopy to discriminate between blueberry fruit and ‘‘foreign substances.’’ They measured the spectra of various foreign substances, including worms, separately from the fruit. They detected a difference in the second derivative of absorbance between worms and berries between approximately 625 and 675 nm.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":"58 1","pages":"370 - 374"},"PeriodicalIF":0.9,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47270601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Suijie Kuang, Yan Tang, Q. Gao, Hua-liang He, W. Ding, Jin Xue, Youzhi Li, Lin Qiu
{"title":"Identification of Potential Target Transcription Factor Genes Regulated by Krüppel Homolog 1 in Chilo suppressalis (Lepidoptera: Crambidae)","authors":"Suijie Kuang, Yan Tang, Q. Gao, Hua-liang He, W. Ding, Jin Xue, Youzhi Li, Lin Qiu","doi":"10.18474/JES22-39","DOIUrl":"https://doi.org/10.18474/JES22-39","url":null,"abstract":"Abstract Juvenile hormone (JH) is a major endocrine hormone that mediates development, metamorphosis, and reproduction in insects. It binds directly to its methoprene-tolerant receptor and recruits a heterodimer partner to form the JH–receptor complex that then activates a JH-inducible gene known as the Krüppel homolog 1 (Kr-h1). There is evidence that this gene is a downstream factor mediating both physiological and biochemical processes; however, the functional mechanism of Kr-h1 is largely unknown. Using the economically important rice (Oryza sativa L.) pest Chilo suppressalis (Walker) (Lepidoptera: Crambidae) as a model, we used a combination of RNA interference (RNAi), high-throughput RNA sequencing, and real-time quantitative polymerase chain reaction (RT-qPCR) to identify candidate transcription factor (TF) genes that are regulated by Kr-h1. RNAi knockdown of Krh1 identified the Zinc finger proteins, ZBTB, THAP, PAX, MYB, HSF, Homeobox, HMG, CSD, basic helix-loop-helix, STAT, RHD, and MBD families as regulated by Kr-h1. RT-qPCR confirmed the transcription levels of these putative TFs and indicated that knockdown of Kr-h1 can induce or suppress the expression of these proteins in C. suppressalis. These results provide the basic information required for in-depth research on the TFs regulated by Kr-h1 in C. suppressalis and other insects.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":"58 1","pages":"318 - 334"},"PeriodicalIF":0.9,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49001815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Temporal and Spatial Expression of Parasitism-Related Olfactory Genes in Larvae of the Ectoparasitoid Dastarcus helophoroides (Coleoptera: Bothrideridae)","authors":"Huayang Yin, Xiaojuan Li, Guang-ping Dong, Wan-lin Guo, Jianmin Fang, Hongjian Liu","doi":"10.18474/JES22-64","DOIUrl":"https://doi.org/10.18474/JES22-64","url":null,"abstract":"Abstract Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) is an ectoparasitoid of the pine sawyer beetle, Monochamus alternatus Hope (Coleoptera: Cerambycidae). A sensitive and precise olfactory system is required for the accurate location of the coleopteran host by D. helophoroides neonates. Herein, we characterized the relative expression patterns of six representative olfactory-related genes at different stages of M. alternatus parasitism and in different body regions of D. helophoroides neonates. The genes encoding chemosensory protein 2 (DhelCSP2), odorant receptor 2 (DhelOR2), and ionotropic receptor 2 (DhelIR2) were significantly upregulated before parasitization was initiated, whereas the genes encoding odorant binding protein 8 (DhelOBP8), gustatory receptor 5 (DhelGR5), and sensory neuron membrane protein 1 (DhelSNMP1) were significantly upregulated 4–5 d after initiation of parasitism. In D. helophoroides neonates, four genes (DhelOBP8, DhelCSP2, DhelOR2, and DhelIR2) were significantly upregulated in the head compared with the thoracoabdominal region, and one gene (DhelGR5) was significantly upregulated in the thoracoabdominal area compared with the head. Double-stranded RNAs (dsRNAs) targeting the six olfactory-related genes were synthesized and delivered to D. helophoroides neonates via immersion. After dsRNA treatment, the transcript levels of four olfactory-related genes (DhelOBP8, DhelCSP2, DhelOR2, and DhelSNMP1) were significantly reduced compared with that of the controls. These results provide a basis for further functional explorations of D. helophoroides olfactory genes, which may lead to the development of improved biological pest control methods using D. helophoroides larvae.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":"58 1","pages":"335 - 343"},"PeriodicalIF":0.9,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42865314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Colpocephalum sp. Chewing Lice (Psocodea: Menoponidae) Collected From Bald Eagles in Mississippi","authors":"J. Goddard, S. Rush, Therese A. Catanach","doi":"10.18474/jes22-73","DOIUrl":"https://doi.org/10.18474/jes22-73","url":null,"abstract":"Bald eagles, Haliaeetus leucocephalus L., are found throughout North America and are often seen in areas where fish (their preferred food) are abundant (Bildstein 2017, Raptors, Cornell Univ. Press, Ithaca, NY). Like other birds, bald eagles may be parasitized by a variety of ectoparasites including lice and mites (Price and Graham 1997, Chewing and Sucking Lice as Parasites of Mammals and Birds, USDA Tech. Bull. No. 1849, Washington, DC; Philips 2000, J. Raptor Res. 34:210– 231). Chewing lice, along with the other parasitic lice, were formerly placed in the insect Order Phthiraptera, but now have been placed within nonparasitic bark lice and book lice in the Order Psocodea (Durden 2019, Lice, Pg. 79–104, G.R. Mullen and L.A. Durden [eds.], Lice, Medical and Veterinary Entomology, 3ed, Elsevier, New York). Five genera of chewing lice, particularly those in the family Menoponidae have been reported previously from bald eagles (Price and Beer 1963, Can. Entomol. 95:731–763; Price et al. 2003, Illinois Natural History Survey Special Publ. No. 24), but records from Mississippi are rare or nonexistent. Here, we report four specimens of Colpocephalum sp. collected from two bald eagles nesting near the Mississippi Gulf Coast. On 8 March 2022, two male bald eagle nestlings were banded by the second author (SR) and his assistants at the Mississippi Sandhill Crane National Wildlife Refuge, Jackson Co., Mississippi, near Gautier (GPS coordinates: 3082730N, 8883930W). During the banding process, an estimated 15 lice were seen running across his fingers and arms; 4 were captured and placed in a vial of alcohol. At the laboratory, all four lice were removed from the alcohol, cleared in a 6.0% potassium hydroxide solution, and mounted on microscope slides for identification using a standard taxonomic key (Fig. 1) (Clay 1969, Bull. British Mus. Nat. Hist. Entomol.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":"58 1","pages":"375 - 376"},"PeriodicalIF":0.9,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46076310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Afsoon Sabet, Erika Dalliance, M. Embers, S. Ward, J. Goddard
{"title":"Attempt to Artificially Infect Cimex lectularius (Hemiptera: Cimicidae) with Bartonella henselae (Alphaproteobacteria: Bartonellaceae)","authors":"Afsoon Sabet, Erika Dalliance, M. Embers, S. Ward, J. Goddard","doi":"10.18474/JES22-57","DOIUrl":"https://doi.org/10.18474/JES22-57","url":null,"abstract":"Abstract Bed bugs (Hemiptera: Cimicidae) are common, hematophagous ectoparasites of humans and other animals and are experiencing an international resurgence. Cimicids have been suspected in the transmission of many disease agents, including Bartonella species; however, disease transmission of any kind has not yet been confirmed in natural disease cycles. Bartonella spp. are transmitted by a variety of arthropods, including fleas, lice, and sand flies, but the potential role of bed bugs in transmission remains unknown. In this study, we used an artificial membrane to feed rabbit blood, either infected or uninfected with Bartonella henselae Regnery et al. (Alphaproteobacteria: Bartonellaceae) to two groups of adult Cimex lectularius L. After 2 wks, the presence of B. henselae in the gut and salivary glands of bugs was assessed via PCR and transmission electron microscopy (TEM), respectively. Although 4 of 10 bed bug guts showed evidence of B. henselae, we were unable to visually detect B. henselae in any of the salivary gland TEM images.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":"58 1","pages":"307 - 317"},"PeriodicalIF":0.9,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47320222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}