Journal of Entomological Science最新文献

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Cotton Aphid (Hemiptera: Aphididae) Resistance to Afidopyropen in Xinjiang Region, China 新疆棉蚜(半翅目:蚜科)对蚜虫的抗性
IF 0.9 4区 农林科学
Journal of Entomological Science Pub Date : 2023-01-24 DOI: 10.18474/JES22-16
Xiaoli Wang, Hui Zha, Quancheng Zhang, Jun-gang Wang
{"title":"Cotton Aphid (Hemiptera: Aphididae) Resistance to Afidopyropen in Xinjiang Region, China","authors":"Xiaoli Wang, Hui Zha, Quancheng Zhang, Jun-gang Wang","doi":"10.18474/JES22-16","DOIUrl":"https://doi.org/10.18474/JES22-16","url":null,"abstract":"Abstract Concentration-mortality response bioassays were conducted in 2021 to define the toxicity of afidopyropen to field populations of cotton aphids, Aphis gossypii Glover (Hemiptera: Aphididae), in Xinjiang region, China. Levels of activity of selected enzymes also were measured. Varying levels of resistance to afidopyropen were detected among aphids from nine major cotton-growing areas in the region. Higher resistance levels were detected in aphids collected from Tumshuk, Alar, and Kurle, with resistance ratios of 4.570, 2.058, and 1.565, respectively. Lower resistance ratios of 0.506, 0.632, and 0.775 were detected in aphids collected from Yinli, Wujiaqu, and Hami, respectively. Biochemical assays showed a highly significant positive correlation of the detoxifying enzymes carboxylesterase and multifunctional oxidase with the level of tolerance, with enzyme activity increasing with the level of tolerance. Glutathione S-transferases and acetylcholinesterase activity in field-collected aphids differed significantly from laboratory colony populations, but their activity did not differ among the field populations. We concluded that cotton aphids in major cotton areas of Xinjiang have not yet developed resistance to afidopyropen and remain sensitive to afidopyropen as indicated by resistance ratios of <5; however, resistance of cotton aphids to afidopyropen in southern Xinjiang is higher than that detected in cotton aphid populations in northern Xinjiang.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44610010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Olfactory Stimulants for Sirex nigricornis (Hymenoptera: Siricidae) and its Parasitoid, Ibalia leucospoides (Hymenoptera: Ibaliidae), in Odors of Stressed and Bark Beetle–Colonized Pines 黑腹夜蛾(膜翅目:夜蛾科)及其寄生蜂白腹夜蛾的嗅觉刺激剂
IF 0.9 4区 农林科学
Journal of Entomological Science Pub Date : 2023-01-24 DOI: 10.18474/JES22-03
William P. Shepherd, C. Johnson, B. Sullivan
{"title":"Olfactory Stimulants for Sirex nigricornis (Hymenoptera: Siricidae) and its Parasitoid, Ibalia leucospoides (Hymenoptera: Ibaliidae), in Odors of Stressed and Bark Beetle–Colonized Pines","authors":"William P. Shepherd, C. Johnson, B. Sullivan","doi":"10.18474/JES22-03","DOIUrl":"https://doi.org/10.18474/JES22-03","url":null,"abstract":"Abstract We conducted studies with the native woodwasp Sirex nigricornis F. (Hymenoptera: Siricidae) in Louisiana to identify host-associated olfactory stimulants that may include attractive semiochemicals for this species as well as Sirex noctilio F., an invasive species that can attack healthy trees. Loblolly pines (Pinus taeda L.) treated with a stressing agent were felled and chipped 1–2 mo after treatment, and the chips were steam–water distilled in a Clevenger apparatus to extract volatile compounds. Using coupled gas chromatography–electroantennographic detection, we analyzed the distillates with antennae of both S. nigricornis and the Sirex parasitoid Ibalia leucospoides (Hochenwarth) (Hymenoptera: Ibaliidae) to identify olfactory stimulants. In addition, we assayed S. nigricornis antennae with synthetic mixtures containing 23 volatile compounds associated with stressed and dying pines, including pheromones of bark beetles (Coleoptera: Curculionidae: Scolytinae) that also use these hosts. Antennae of both male and female S. nigricornis responded to 29 identifiable volatiles in the distillates, whereas I. leucospoides responded to 23 volatiles. Eighteen compounds in the synthetic mixtures were olfactory stimulants for S. nigricornis. Olfactory stimulants in the woodchip distillates were predominantly hydrocarbon and oxygenated monoterpenes, and the strongest antennal stimulants among compounds in the synthetic mixtures were oxygenated monoterpenes associated with pine death and early decay (e.g., verbenone, fenchone, and terpinen-4-ol). Bark beetle pheromones (frontalin, endo-brevicomin, ipsenol, and ipsdienol) also stimulated S. nigricornis antennae. The very large number of host-associated olfactory stimulants for S. nigricornis implies that our data may offer limited assistance in targeting individual compounds for investigation as possible attractants and components for a Sirex lure.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46927024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression Patterns of Heat Shock Protein Genes and Antioxidase Genes in Apis cerana cerana (Hymenoptera: Apidae) under Heat Stress 热应激条件下中华蜜蜂热休克蛋白基因和抗氧化酶基因的表达模式
IF 0.9 4区 农林科学
Journal of Entomological Science Pub Date : 2023-01-24 DOI: 10.18474/JES22-27
Xinyu Li, Weihua Ma, Yusuo Jiang
{"title":"Expression Patterns of Heat Shock Protein Genes and Antioxidase Genes in Apis cerana cerana (Hymenoptera: Apidae) under Heat Stress","authors":"Xinyu Li, Weihua Ma, Yusuo Jiang","doi":"10.18474/JES22-27","DOIUrl":"https://doi.org/10.18474/JES22-27","url":null,"abstract":"Abstract Heat stress elicits the expression of various antistress proteins (e.g., heat shock proteins [HSPs] and antioxidase enzymes) in honeybees (Hymenoptera: Apidae), which are important in protecting cells from heat-induced stresses. In this study, we used real-time quantitative reverse transcription–polymerase chain reaction to analyze the expression patterns of the heat shock protein 90 (Hsp90), heat shock protein 70Ab (Hsp70Ab), peroxidase (Pod), and cytochrome P450 (Cyp450) in response of Apis cerana cerana F. to different temperatures and different heat exposure times. We observed that, with the increase of temperature and exposure time, the expression of the four genes also increased, thus confirming that heat stress can activate heat-resistant mechanisms of A. cerana cerana and that temperature and exposure time are key factors affecting the accumulation of HSPs. Our results provide information on the expression patterns of four genes during heat stress to serve as a basis for determining the mechanisms by which A. cerana cerana adapts to thermal stress.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42317742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Insecticidal Activity of Essential Oil from Seeds of Foeniculum vulgare (Apiales: Apiaceae) Against Sitophilus zeamais (Coleoptera: Curculionidae) and Its Effects on Crop Seed Germination 小茴香种子精油对玉米象虫的杀虫活性及其对作物种子萌发的影响
IF 0.9 4区 农林科学
Journal of Entomological Science Pub Date : 2023-01-24 DOI: 10.18474/JES22-13
R. Wanna, Phirayot Khaengkhan
{"title":"Insecticidal Activity of Essential Oil from Seeds of Foeniculum vulgare (Apiales: Apiaceae) Against Sitophilus zeamais (Coleoptera: Curculionidae) and Its Effects on Crop Seed Germination","authors":"R. Wanna, Phirayot Khaengkhan","doi":"10.18474/JES22-13","DOIUrl":"https://doi.org/10.18474/JES22-13","url":null,"abstract":"Abstract Many aromatic herbs in the family Apiaceae produce essential oils that are used on an industrial scale for medicinal, cosmetic, and food purposes. Essential oils from plants that show insecticidal activity can be substituted for synthetic insecticides to reduce environmental pollution or harmful toxicity to humans. Insecticidal activity of essential oil from seeds of coriander, Foeniculum vulgare Miller (Apiales: Apiaceae), on maize weevil, Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), and its effects on seed germination of three major economic crops were investigated. Essential oil from F. vulgare seeds was analyzed by gas chromatography–mass spectrometry and found to contain 25 components, with anethole (43.78%) as the main constituent. Other basic compounds were estragole (28.33%), fenchone (16.68%), d-limonene (2.62%), alpha-pinene (1.84%), and p-cymene (1.28%). Foeniculum vulgare essential oil was toxic to maize weevil when used as a fumigant with a median lethal concentration at 48 h of 10.42 µL/L air. A concentration of 64 µL/L air was most effective, killing 100% of maize weevil within 24 h. The repellent effect on maize weevil increased to 75% at 24 h at a concentration of 16 µL/L air. Rice (Oryza sativa L.) and sorghum (Sorghum bicolor [L.] Moench) seeds fumigated with 32 µL/L air of F. vulgare essential oil exhibited a slight decrease in germination rate (germination >90%). Our results indicate that essential oil from F. vulgare seeds have potential for application in the management of maize weevil in stored products.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44025478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Phototactic Responses of Three Species of Cryptolestes (Coleoptera: Laemophloeidae) to Different Wavelengths of Light 三种隐蝇(鞘翅目:隐蝇科)对不同波长光的趋光反应
IF 0.9 4区 农林科学
Journal of Entomological Science Pub Date : 2023-01-24 DOI: 10.18474/JES22-24
M. Cui, Yi Wu, L. Xia, Dingrong Xue, Zhongming Wang
{"title":"Phototactic Responses of Three Species of Cryptolestes (Coleoptera: Laemophloeidae) to Different Wavelengths of Light","authors":"M. Cui, Yi Wu, L. Xia, Dingrong Xue, Zhongming Wang","doi":"10.18474/JES22-24","DOIUrl":"https://doi.org/10.18474/JES22-24","url":null,"abstract":"Abstract Information on insect phototaxis can be applied to the collection, monitoring, and management of insects. This study investigated the phototaxis of three species of Cryptolestes (Coleoptera: Laemophloeidae)—Cryptolestes pusillus (Oliver), Cryptolestes turccus (Grouvelle), and Cryptolestes ferrugineus (Stephens)—to light-emitting diode (LED) lights with six wavelengths. The three species had different responses to the different wavelengths, with C. pusillus displaying the strongest phototaxis. The taxis of the three species exhibited the same tendency across light waves of different lengths. All showed positive taxis to 450-, 390-, and 530-nm wavelengths, and their highest taxis indices occurred with 450 nm. The taxis indices of all three Cryptolestes species were positively correlated to light intensity.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43850604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An Annotated Checklist of the Jewel Beetles (Coleoptera: Buprestidae) of Doña Ana County, New Mexico 新墨西哥州多纳阿纳县宝石甲虫(鞘翅目:蟾蜍科)注释清单
IF 0.9 4区 农林科学
Journal of Entomological Science Pub Date : 2023-01-24 DOI: 10.18474/JES22-15
Joel W. DuBois, W. P. Steffens, C. Bundy
{"title":"An Annotated Checklist of the Jewel Beetles (Coleoptera: Buprestidae) of Doña Ana County, New Mexico","authors":"Joel W. DuBois, W. P. Steffens, C. Bundy","doi":"10.18474/JES22-15","DOIUrl":"https://doi.org/10.18474/JES22-15","url":null,"abstract":"Abstract An annotated checklist of the jewel beetles (Coleoptera: Buprestidae) of Doña Ana County, New Mexico is provided. Records compiled from museum specimens, available literature, and field collections yielded 79 species representing 21 genera and 4 subfamilies. The greatest number of species were recorded from the subfamilies Polycestinae (31 species) and Buprestinae (25 species). Acmaeodera yuccavora Knull (Polycestinae) and Agrilus fisherellus Obenberger (Agrilinae) represent two new state records in New Mexico.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41484353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nilaparvata lugens (Hemiptera: Delphacidae) Midgut Microbial Community Responses to Exposure to Metarhizium flavoviride (Sordariomycetes: Hypocreales) 褐飞虱(半翅目:褐飞虱科)中肠微生物群落对黄绿绿僵菌的反应
IF 0.9 4区 农林科学
Journal of Entomological Science Pub Date : 2022-10-12 DOI: 10.18474/JES22-11
J. Zhang, F. Li, H. Zhong, J.M. Chen
{"title":"Nilaparvata lugens (Hemiptera: Delphacidae) Midgut Microbial Community Responses to Exposure to Metarhizium flavoviride (Sordariomycetes: Hypocreales)","authors":"J. Zhang, F. Li, H. Zhong, J.M. Chen","doi":"10.18474/JES22-11","DOIUrl":"https://doi.org/10.18474/JES22-11","url":null,"abstract":"Abstract Changes to the community of symbiotic bacteria and fungi in the brown planthopper, Nilaparvata lugens Stål, were measured at different times after exposure to conidial suspensions of Metarhizium flavoviride Gams and Roszypal. Over all concentrations tested, bacterial species associated with N. lugens comprised 8 phyla, 17 classes, 22 orders, 26 families, and 31 genera of which the relative proportions of Arsenophonus, Burkholderia, Enterobacter, Pseudomonas, Stenotrophomonas, and Bacteroides were highest. The relative abundance and diversity of bacteria were highest in the carrier control (0.05% Tween-80 only). Fungi comprised 1 phyla, 5 classes, 10 orders, 13 families, and 18 genera, with Metarhizium being the dominant taxon in specimens from all treatment groups. Metarhizium spp. was greatest in the concentrations of 107 and 108 conidia/ml at 72 hours after exposure, reaching 94.82% and 93.74% of taxonomic units, respectively. We deduced that M. flavoviride competes for nutrition with midgut microorganisms; therefore, exposure to a pathogenic fungus will change the abundance and diversity of bacterial and fungal microorganisms in the midguts of hosts, and pathogens will impact the structure of bacterial communities in the host midgut with an alteration in the bacterial species composition. We observed that following the exposure of N. lugens to M. flavoviride, Metarhizium spp. dominated in the midgut of the host, the abundance and diversity of midgut fungal microorganisms decreased, and the dominant bacterial species in the midgut shifted.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43157441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of Electron Beam Irradiation on the Developmental Stages of Callosobruchus maculatus (Coleoptera: Bruchidae) and Bactrocera zonata (Diptera: Tephritidae) 电子束辐照对斑点小茧蜂(鞘翅目:帚虻科)和带小茧蜂(双翅目:绢虻科)发育阶段的影响
IF 0.9 4区 农林科学
Journal of Entomological Science Pub Date : 2022-10-12 DOI: 10.18474/JES21-72
A. Gabarty, A. Hammad, R. Zinhoum, A. Negm
{"title":"Impact of Electron Beam Irradiation on the Developmental Stages of Callosobruchus maculatus (Coleoptera: Bruchidae) and Bactrocera zonata (Diptera: Tephritidae)","authors":"A. Gabarty, A. Hammad, R. Zinhoum, A. Negm","doi":"10.18474/JES21-72","DOIUrl":"https://doi.org/10.18474/JES21-72","url":null,"abstract":"Abstract An eco-friendly electron beam irradiation (EBI) technology was used to assess the effect of EBI on the different development stages of Callosobruchus maculatus (F.) and Bactrocera zonata (Saunders). No adults emerged after 3-d-old eggs of C. maculatus were irradiated with 304.8 Gy of EBI, and no adults emerged from the F1 generation of larvae and pupae irradiated with 103.6 Gy. The adult stage of C. maculatus was the most tolerant of EBI, with a high dose (414.3 Gy) irradiation of the adult stage resulting in complete prevention of the F1 generation adult emergence. Large-scale tests confirmed that 414.3 Gy was an effective dose for the phytosanitary and security treatment for C. maculatus. An EBI dose of 414.3 Gy prevented B. zonata egg hatching. EBI doses of 304.8, 414.3, and 653.5 Gy prevented the development of first, second, and third larval instars to pupation, respectively. Using the criterion of adult emergence from the F1 generation, targeting the third larval stage, considered the most tolerant stage to EBI, a dose of 304.8 Gy was effective for quarantine and security treatment of B. zonata.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46146456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Emergence and Reproductive Rhythm of Clostera anastomosis (Lepidoptera: Notodontidae) 梭翅目吻合昆虫的出现与繁殖节律(鳞翅目:齿蛾科)
IF 0.9 4区 农林科学
Journal of Entomological Science Pub Date : 2022-10-12 DOI: 10.18474/JES21-70
Luo Peng, C. Lihui, Chenato Lin, W. Guangli
{"title":"Emergence and Reproductive Rhythm of Clostera anastomosis (Lepidoptera: Notodontidae)","authors":"Luo Peng, C. Lihui, Chenato Lin, W. Guangli","doi":"10.18474/JES21-70","DOIUrl":"https://doi.org/10.18474/JES21-70","url":null,"abstract":"Abstract Clostera anastomosis L. (Lepidoptera: Notodontidae) is a serious defoliator of poplar, Populus spp., trees in China. To establish a baseline of information for possible ecological management of this pest, we studied its emergence, courtship, mating, and oviposition behaviors in the laboratory at 27 ± 1°C and 60 ± 10% relative humidity under a 14:10-h (light:dark) photoperiod. Under these conditions, peak emergence of female adults occurred in the sixth day after pupation, and peak emergence of males was in the seventh day after pupation. Emergence for both sexes occurred throughout the day. Courtship behavior began at the sixth hour of scotophase, reaching peak activity between the 10th hour of scotophase and the first hour of photophase. The courtship and mating success rate were highest for 1-d-old females, and then gradually decreased with age until no mating was observed after females were 4 d old. With 1-d-old females, mating was only observed from the seventh hour of scotophase to the first hour of photophase, with peak activity at 0.5 h after the onset of photophase. Oviposition primarily occurred within 3 d after mating, with 49.8% of the eggs being deposited during the first day after mating. These results demonstrate that there are distinct circadian rhythms in adult emergence and subsequent reproductive behavior of C. anastomosis, thus providing a basis for development of monitoring and management strategies of this pest.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47659570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Innate Immune Response of Galleria mellonella (Lepidoptera: Pyralidae) Larvae to Lemongrass Essential Oil and Citral 香茅精油和柠檬醛对黄颡鱼蛾(鳞翅目:蚜科)幼虫的先天免疫反应
IF 0.9 4区 农林科学
Journal of Entomological Science Pub Date : 2022-10-12 DOI: 10.18474/JES22-08
Kyndra Chastain, Westley Peterson, Katherine Haszcz, Michaela Fenske, J. Rice, M. Pszczolkowski
{"title":"Innate Immune Response of Galleria mellonella (Lepidoptera: Pyralidae) Larvae to Lemongrass Essential Oil and Citral","authors":"Kyndra Chastain, Westley Peterson, Katherine Haszcz, Michaela Fenske, J. Rice, M. Pszczolkowski","doi":"10.18474/JES22-08","DOIUrl":"https://doi.org/10.18474/JES22-08","url":null,"abstract":"Abstract The innate immune response in insects relies on cellular responses. In most lepidopteran species, challenging the immune system causes an increase in hemocyte numbers and promotes encapsulation and melanization of invading objects, such as parasitoid eggs or artificial objects (e.g., nylon implants). We studied the effects of carbon particles (Sumi ink) on the immune response of Galleria mellonella L. (Lepidoptera: Pyralidae) last-instar larvae. Ink injections increased total hemocyte counts in a dose-dependent manner, with the 40 and 80% concentrations being most effective in eliciting hemocyte response. Hemocyte numbers increased significantly at 4 and 6 h after ink injection. Lemongrass essential oil (0.18, 0.36, and 0.72 µg/larva) and citral (0.12, 0.24, and 0.48 µg/larva) reversed the mobilization of hemocytes by the ink. Melanization of nylon larvae implants started as early as 15 min after implant insertion and reached maximum observed levels within 1 h. The degree of melanization also was higher in larvae challenged with ink 1 h prior to implant removal, which suggests initialization of the immune response. Citral injections inhibited implant melanization both in unchallenged and ink-challenged larvae. In the former case, citral concentrations needed for effective inhibition were less (0.3 µg/larva) than in ink-challenged larvae (1.2 and 2.4 µg/larva).","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42922490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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