Journal of Environmental Chemical Engineering最新文献

{"title":"Electrode materials for copper removal from wastewater by bioelectrochemical systems: A review","authors":"Han Zhang ,&nbsp;Lequn Sun ,&nbsp;Shuai Luo ,&nbsp;Rongfang Yuan ,&nbsp;Rongrong Hou ,&nbsp;Beihai Zhou ,&nbsp;Huilun Chen","doi":"10.1016/j.jece.2025.116148","DOIUrl":"10.1016/j.jece.2025.116148","url":null,"abstract":"<div><div>Copper-containing wastewater is produced in large quantities, threatening the environment yet having high recycling value. Proper disposal of copper in wastewater is crucial for environmental protection. The bioelectrochemical system (BES), including microbial fuel cells (MFC), microbial electrolysis cells (MEC), and microbial desalination cells (MDC), can remove pollutants and recover resources in wastewater via microorganisms and electric currents. Electrode materials, a key component, directly affect reactor efficiency and can be classified into carbon - based, metal, and modified electrodes, etc. Although research on BES treating copper - containing wastewater is increasing, there's no summary. This article fills the gap, focusing on BES reactor concepts, working principles, and development history; practical cases of treating copper - containing wastewater; and the impact of electrode materials and operating parameters on BES performance. Results show BES is a green, pollution - free, and efficient reactor, effective for treating copper - containing wastewater and promising for copper recovery. It also discusses reasons for current lab - stage research and difficulties in engineering applications, and proposes future development directions.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 3","pages":"Article 116148"},"PeriodicalIF":7.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143641873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treating domestic wastewater towards freshwater quality: Bacterial community and antibiotic resistance profiles highlight critical steps and improvement opportunities","authors":"Inês Leão ,&nbsp;Jorge Antunes ,&nbsp;Inês Baptista ,&nbsp;Ruben Jorge ,&nbsp;Luís Marinheiro ,&nbsp;Stefan Löblich ,&nbsp;Ivone Vaz-Moreira ,&nbsp;Célia M. Manaia","doi":"10.1016/j.jece.2025.116172","DOIUrl":"10.1016/j.jece.2025.116172","url":null,"abstract":"<div><div>Ideally, wastewater treatment aims to produce water indistinguishable from freshwater, especially for reuse. This study evaluated bacterial community and antibiotic resistance variations throughout treatment and benchmarked these with freshwater sources. Samples collected from six points of a full-scale wastewater treatment plant, pilot-scale advanced treatment options (non-thermal plasma - NTP, ultrafiltration - UF, UF followed by reverse osmosis- UF+RO), two rivers and a borehole were analyzed for quality parameters (BOD5, TSS, turbidity, <em>Escherichia coli</em>), antibiotic resistance genes (quantitative PCR), class 1 integron variable region composition (Oxford Nanopore sequencing), and bacterial community composition (16S rRNA Illumina sequencing).</div><div>Secondary treatment followed by sand filters and coagulants caused the highest reduction (∼2 log-unit/volume) of all analyzed parameters and the sharpest reduction of diversity of antibiotic resistance genes within class 1 integrons’ variable region. Ultraviolet disinfection triggered minimal bacterial or genes reduction, while among advanced treatments, UF+RO caused the highest, and NTP the lowest.</div><div>Principal component analysis suggested significant associations between antibiotic resistance (n = 32) and genetic recombination elements (n = 12) and predominant bacterial families in raw wastewater (<em>Aeromonadaceae</em>, <em>Moraxellaceae</em>, <em>Campylobacteraceae</em>, <em>Lachnospiraceae</em>). For predominant freshwater families (<em>Comamonadaceae</em>, <em>Chitinophagaceae</em>, <em>Flavobacteriaceae</em>) no significant associations were observed. Freshwater differed from UF-treated water by a lower antibiotic resistance abundance, higher bacterial richness (∼4000 vs.1200 operational taxonomic units) and distinct predominant families - <em>Alcaligenaceae</em>, <em>Sphingomonadaceae</em>, <em>Chitinophagaceae</em>, and <em>Microbacteriaceae</em> in UF water. The findings underscore the critical role of secondary/post-secondary treatments in shaping resistance and community profiles and suggest that advanced treatment should balance water quality with bacterial diversity preservation for sustainable reuse.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 3","pages":"Article 116172"},"PeriodicalIF":7.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143641762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2D – layer of graphene oxide incorporated with transition metal atoms of MnSnO2 for the electrochemical detection of anti-androgen drug nilutamide in environmental samples","authors":"Nandhini Munusamy ,&nbsp;Sri Balaji Natarajan ,&nbsp;Tse-Wei Chen ,&nbsp;Sivaprakash Sengodan ,&nbsp;Jaysan Yu ,&nbsp;Shen-Ming Chen","doi":"10.1016/j.jece.2025.116158","DOIUrl":"10.1016/j.jece.2025.116158","url":null,"abstract":"<div><div>The Bi-metallic nanocomposite of MnSnO₂ combined with graphene oxide (GO) is used for the electrochemical detection of the anti-androgen drug Nilutamide (NLT). The material is synthesized via hydrothermal methods and can be calcined for structural and morphological analysis. Upon incorporation with GO, the material’s electrocatalytic performance and electrical conductivity are enhanced. Physical characterization of MnSnO₂@GO is performed using X-ray diffraction (XRD) to determine crystal structure and plane, Fourier Transform Infrared Spectroscopy (FT-IR) for functional group identification, Raman spectroscopy, and X-ray Photoelectron Spectroscopy (XPS) to analyze oxidation states of elements. Morphological studies are conducted using Field Emission-Scanning Electron Microscopy (FE-SEM) and High-Resolution Transmission Electron Microscopy (HR-TEM). The electrochemical sensor detects NLT, with differential pulse voltammetry (DPV) showing a linear range of 0.01–965 µM, a sensitivity of 0.01 µA µM⁻¹ cm⁻², and a low detection limit of 0.098 µM. NLT detection is also tested in real samples, including food.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 3","pages":"Article 116158"},"PeriodicalIF":7.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced transformation of sulfadiazine by horseradish peroxidase activated persulfate progress: Characteristics, pathways, mechanisms, and ecotoxicities","authors":"Yifei Leng ,&nbsp;Ying Zhang ,&nbsp;Xiong Chen ,&nbsp;Ting He ,&nbsp;Fengyi Chang ,&nbsp;Zhu Li ,&nbsp;Yu Huang ,&nbsp;Jun Wang ,&nbsp;Wen Xiong","doi":"10.1016/j.jece.2025.116140","DOIUrl":"10.1016/j.jece.2025.116140","url":null,"abstract":"<div><div>The catalysis of horseradish peroxidase (HRP) and activated persulfate (PDS) are both feasible ways to remove heterologous contaminants. This work demonstrated that HRP/H₂O₂ can effectively activate PDS to degrade sulfadiazine (SDZ). Types of salt ions could suppress the efficiency of the system through free radical scavenging of anions and superposition effect of part cations, followed the series: FeCl₃ &gt; CaCl₂&gt; NaCl &gt; NH₄Cl &gt; NaHCO₃ &gt; NaNO₃. 5 mg L⁻¹ humic acid (HA) could decrease 5.14 times transformation rate constants <em>K</em>. Interestingly, the addition of the above salt ion can slightly restore the level of inhibition by HA. Fourteen possible intermediates were identified, and potential pathways and mechanisms were proposed. There were three pathways to transform SDZ in systems. HRP directly acted on SDZ after being activated by H₂O₂, including δ/γ site-cleavage, hydroxylation, denitrification, carbonylation, and sulfur oxide (SO₂/SO₃) extrusion. In addition, both HRP activated PDS and thermal/H₂O₂, generating SO₄^-• and •OH for the oxidation of SDZ, including oxidation of the α-amine, hydroxylation of the benzene ring, ζ position cleavage on SDZ. The electron paramagnetic resonance (EPR) technique verified that specific free radicals had been generated and that HRP could activate the PDS process. The ECOSAR software predicted the biotoxicity of most transformation products was less toxic than the parent compound. The results of luminescence inhibition of <em>Vibrio fischeri</em> indicated the low toxicity of SDZ intermediates. These findings may help us expand our knowledge of PDS activation approaches and may also provide a potential method for antibiotic removal in environments.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 3","pages":"Article 116140"},"PeriodicalIF":7.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143628759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rigid polyelectrolyte multilayer membrane with ultrahigh permeance and excellent dye/salt separation performance","authors":"Na Zhang ,&nbsp;Fanglu Fu ,&nbsp;Wen Bo ,&nbsp;Hussein Baqiah ,&nbsp;Li Zhao ,&nbsp;Wenbo Lu ,&nbsp;Yadi Cai ,&nbsp;Jiahui Tian ,&nbsp;Tingting Yan ,&nbsp;Saisai Li ,&nbsp;Shuyun Zheng ,&nbsp;Qiang Li","doi":"10.1016/j.jece.2025.116164","DOIUrl":"10.1016/j.jece.2025.116164","url":null,"abstract":"<div><div>Two kinds of polyelectrolyte assembled membranes have been prepared using a rigid sulfonated polysulfone (SPSU) and a flexible poly(sodium 4-styrenesulfonate) (PSS) as anionic polyelectrolytes, respectively, with a polyethyleneimine (PEI) as a common cationic polyelectrolyte. The effects of molecular backbone’s rigidity and flexibility on the characteristics and performances of SPSU/PEI and PSS/PEI membranes have been investigated in detail. The SPSU/PEI membrane with two bilayers (SPSU/PEI-2.0) has a thinner and looser separation layer due to expanded molecular conformation and bulky benzene rings of the SPSU. In addition, the SPSU/PEI-2.0 membrane exhibits an ultrahigh pure water permeance up to 70.0 L m⁻² h⁻¹ bar⁻¹. The optimized separation performance for Congo red (CR)/NaCl and CR/Na₂SO₄ displays the same high CR rejection of 99.6 %, low NaCl rejection of 1.2 % and Na₂SO₄ rejection of 7.9 %. The SPSU/PEI-2.0 membrane also shows excellent acid-resistance stability, thermal stability and long-term operation stability. This research presents that choosing polyelectrolytes with appropriate molecular structures can be attractive when designing polyelectrolyte multilayer membrane to separate dye and salt.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 3","pages":"Article 116164"},"PeriodicalIF":7.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment and understanding of the impact of zwitterionization of polyethersulfone on the fouling of the polymeric membranes used for ultrafiltration of abattoir wastewater","authors":"Mabore J. Raseala ,&nbsp;Funeka Matebese ,&nbsp;Mxolisi M. Motsa ,&nbsp;Rudzani A. Sigwadi ,&nbsp;Richard M. Moutloali","doi":"10.1016/j.jece.2025.116120","DOIUrl":"10.1016/j.jece.2025.116120","url":null,"abstract":"<div><div>Ultrafiltration is a promising technique applied in wastewater treatment. The potential application of UF in abattoir wastewater treatment has not been given much attention due to its drawback of being prone to organic fouling. To be efficient, it is necessary to assess fouling mechanisms involved to establish suitable mitigation actions, which is not widely reported. Membrane zwitterionization has proven efficient in mitigating membrane organic fouling through hydration layer formation that acts as a barrier. An understanding of the fouling mechanism of membranes assists with the overall optimum application of polymeric membranes. Herein, a thorough design of experiments was carried out to investigate the effects of the zwitterionization of PES on permeate flux and its fouling mechanisms of the membranes. Hermia models were used to elucidate fouling mechanisms in the study. The results of the Hermia model showed that the hydration layer formed delayed the pollutant-membrane surface interaction, leading to smaller molecules behaving like larger molecules. As a result, it was observed that both Z2 and Z4 membranes followed intermediate mechanism prior to the formation of the cake layer. Whereas, pristine favoured mostly the standard mechanism of fouling due to the absence of any barrier between the surface of the membrane and the pollutants, resulting in the narrowing of the pores and a faster flux decline. Furthermore, after backwashing with deionised water, the zwitterionic membranes exhibited reversible fouling. These observations imply that zwitterionic membranes have the potential to be more cost-effective and have a longer lifespan than the pristine PES membranes.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 3","pages":"Article 116120"},"PeriodicalIF":7.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143641874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Degradable itaconic acid-based polyurethane with excellent sustained release of urea for agricultural applications","authors":"Yuqing Wang ,&nbsp;Mengqiu Quan ,&nbsp;Genzheng Sha ,&nbsp;Junming Huang ,&nbsp;Minghui Cui ,&nbsp;Xiaoqing Li ,&nbsp;Jin Zhu ,&nbsp;Jing Chen","doi":"10.1016/j.jece.2025.116131","DOIUrl":"10.1016/j.jece.2025.116131","url":null,"abstract":"<div><div>Thermoset polyurethanes find applications in various industries due to their exceptional performance resulting from their permanent cross-linked structure. However, thermoset polyurethanes are all obtained by chemical cross-linking, and their permanent network structure cannot be recycled or reprocessed. This results in the waste of non-renewable petroleum resources and environmental pollution. Therefore, preparing polyurethane (PU) from biomass has become a key focus of attention. This study synthesized itaconic acid-based polyols from itaconic acid (IA) and 1-thioglycerol (TG) by a thiol-ene photoclick reaction. Subsequently, we synthesized itaconic acid-based polyurethane materials by adjusting the ratio of soft and hard segments. The materials displayed excellent mechanical and degradation properties, with a maximum tensile strength of 50.61 MPa and a remaining mass of 56.79 % at 126 days for IATG-PUs-3. Urea was coated on itaconic acid-based polyurethane (PU) to facilitate its function as a slow-release fertilizer. The release rate in soil over 30 days was 81.63 %. Based on this, we conducted an indoor potting test to investigate the growth-promoting effect of slow-release fertilizer on Chinese little greens and Little bok choy. It found that covering the plants with a film of IATG-PUUs significantly improved their growth indices. Furthermore, the growth indices of plants covered with an IATG-PU film were higher than those not mulched. This approach presents a practical solution for creating bifunctional membranes with slow-release fertilizer and biodegradation properties.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 3","pages":"Article 116131"},"PeriodicalIF":7.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breaking the pH limitation by Mo modulated amorphous medium-entropy alloys as efficient advanced oxidation catalysts","authors":"Xilin Wu ,&nbsp;Shun-Xing Liang ,&nbsp;Lingjie Jia ,&nbsp;Yufeng Liu ,&nbsp;Yuexian Huang ,&nbsp;Yujing Liu ,&nbsp;Liang-Yu Chen ,&nbsp;Yuanzheng Yang ,&nbsp;Pingjun Tao","doi":"10.1016/j.jece.2025.116151","DOIUrl":"10.1016/j.jece.2025.116151","url":null,"abstract":"<div><div>The inherent limitations of conventional metallic glass (MG) catalysts in adapting to complex water environments over a wide pH range stem from their monotonic active site, which is incapable of simultaneously fulfilling multiple functional purposes. Herein, we overcome the narrow range of pH adaptability of existing advanced oxidation catalysts by designing a series of quinary FeCoNiMoB medium-entropy alloys in amorphous structure (referred to as A-MEAs). In particular, the slight modulation of Mo to obtain Fe₂₅Co₂₅Ni₂₅Mo_0.5B_24.5 A-MEA with highest Gibbs free energy achieves a complete degradation of pollutants both at acidic and alkaline conditions in 10 min. This performance surpasses that of most traditional MGs and high-entropy alloys (HEAs) constrained by the composition limits for entropy maximization. Further insights reveal that in both cases, the multi-site synergistic effects of Mo-driven fast electron transfer of M (M = Fe, Co, and Ni) as active sites and surface-mediated reaction cycles of M^2 +/M³⁺ contribute to the accelerated transformation of reactive oxygen species (ROS) from peroxydisulfate (PDS) and the remarkable catalytic performance of A-MEAs. The radical evolution demonstrates that SO₄·‾ plays a major role under acidic conditions while O₂·‾ dominates the catalytic reactions under alkaline conditions. Accordingly, this work aims to fill the gap of A-MEAs for catalytic oxidation of organic pollutants and provide the design strategy of novel MEA catalysts.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 3","pages":"Article 116151"},"PeriodicalIF":7.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143641867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence and distribution of PAHs and indigenous bacteria in the soil surrounding the industrial parks of Beijing: A systematic field study","authors":"Yaling Gou,&nbsp;Pengwei Qiao,&nbsp;Peizhong Li,&nbsp;Wenxia Wei,&nbsp;Xiang Li,&nbsp;Jiajia Wang,&nbsp;Nan Luo,&nbsp;Yun Song","doi":"10.1016/j.jece.2025.116127","DOIUrl":"10.1016/j.jece.2025.116127","url":null,"abstract":"<div><div>Several studies have been devoted to the characterization of soil PAHs pollution in decommissioned industrial sites and their impacts on soil microbial community, however, the investigation on the pollutant levels and associated indigenous microbial communities of the soil surrounding the industrial parks were scare. In this study, 4 typical industrial parks of Beijing that had previously confirmed polycyclic aromatic hydrocarbons (PAHs) pollution were selected and resampled to systematically quantify the PAHs pollution and investigate their impact on indigenous bacterial community and function diversity. The detection frequency of PAHs among 76 soils is 5.26 %, the maximum of total PAHs is 8.33 mg kg^–1. Principal co-ordinates analysis (PCoA) results showed that the soil bacterial communities and functions among different industrial parks, locations, or distance from the boundary of respective industrial park were not showed significant differences (p &gt; 0.05). The network analysis results showed that the complexity, natural connectivity and stability of the networks followed the order of FSGY &gt; JJJS &gt; LHJJ &gt; YSSH, which revealed that the PAHs exposure caused some stress to soil indigenous microbes. The main factor impacting bacterial communities and functions is total PAHs in the soil, which increased the alpha diversity of bacterial communities, while decreased the complexity and stability of respective network. Distance-decay relationship (DDR) analysis identified geographical distance as the primary driver on soil bacterial community compared with environmental variability. Our results will enhance the understanding of PAHs pollution and the indigenous bacteria characteristic of the soil surrounding industrial parks and favor the sustainable management of industrial parks.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 3","pages":"Article 116127"},"PeriodicalIF":7.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated, single-reactor for double-duty chemoautotrophic sulfide bio-oxidation and sulfate bio-reduction: Key parameters for unmatched efficiency in elemental sulfur production","authors":"Hawzhin Amanollahi ,&nbsp;Gholamreza Moussavi ,&nbsp;Stefanos Giannakis","doi":"10.1016/j.jece.2025.116107","DOIUrl":"10.1016/j.jece.2025.116107","url":null,"abstract":"<div><div>The present study proposes an integrated system for the simultaneous removal of sulfide and sulfate, and determined the key parameters for high-level production of elemental sulfur (S⁰) as the desired end-product . Various modes of operation, including batch, semi-continuous, and continuous, were also examined. The packed bed bioreactor (PBBR) was operated under oxygen-limited conditions with different hydraulic retention times (HRT) near-neutral pH. At an HRT of 3 h, removal efficiencies greater than 99 % and 98 % were achieved for S^2- bio-oxidation and SO₄^2- bio-reduction, respectively. During the semi-continuous mode of PBBR operation, thiosulfate (S₂O₃^2-), a metabolite of sulfur oxidation, was detected in the effluent; however, when the bioreactor was operated in continuous mode at an HRT of 3 h, the concentration of thiosulfate was below the detection limit. The highest S⁰ selectivity (45 %) was achieved at an HRT of 1 h. Next-generation sequencing (NGS) analysis was used to identify the diversity of bacterial species in the biofilm; <em>Thiobacillus sajanensis</em>, <em>Thiobacillus thioparus</em>, and <em>Rhizobium arsenicireducens</em> were found to be the dominant species responsible for the simultaneous biological conversion of sulfide and sulfate. The mechanism of simultaneous sulfide and sulfate bioconversion was investigated and proposed. In summary, the developed PBBR was an efficient method for the concurrent bioconversion of sulfide and sulfate, with considerable selectivity towards elemental sulfur.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 3","pages":"Article 116107"},"PeriodicalIF":7.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}