Journal of Environmental Chemical Engineering最新文献

{"title":"Revealing the oxidative stress mechanism induced by defect engineering of magnesium oxide nanoparticles under dark conditions","authors":"Jitao Liu ,&nbsp;Baixue Ouyang ,&nbsp;Wei Dun ,&nbsp;Peng Chen ,&nbsp;Tingzheng Zhang ,&nbsp;Haoran Dong ,&nbsp;Yuewen Qing ,&nbsp;Weifang Liu ,&nbsp;Yingjie He ,&nbsp;Haiying Wang","doi":"10.1016/j.jece.2025.119192","DOIUrl":"10.1016/j.jece.2025.119192","url":null,"abstract":"<div><div>The spread of drug-resistant bacteria poses a serious threat to public health. Nano-sized MgO exhibits excellent biocompatibility, low toxicity, and broad-spectrum antibacterial effects. Its ability to generate reactive oxygen species (ROS) even in dark conditions makes it a promising antibacterial material. However, its production of ROS is limited, and the mechanism of ROS generation under dark conditions is not yet clear, which restricts its practical application. To address this challenge, we have developed a metal-doped MgO nanomaterial with enhanced ROS generation capability. The use of spray drying simplifies the preparation of the nanoparticles, while high-temperature calcination can facilitate the effective substitution of external metal ions into the MgO crystal lattice. Doping with foreign metals do not compromise the inherent biocompatibility of MgO. A key aspect is that differences in ionic radius and charge among the doped metal ions induce the detachment of oxygen molecules from the MgO surface and cause lattice distortions, resulting in additional surface oxygen vacancies. The increased concentration of surface oxygen vacancies enhances electron transfer on the material’s surface, thereby promoting the generation of ROS. By doping with Li of similar radius but lower valence state to induce lattice defects, LiMgO (0.4 mg/mL) can inactivate more than 99.99 % of <em>Escherichia coli</em> (<em>E. coli</em>) with a concentration of 10⁸ cfu/mL within 15 min through the physical contact and oxidation mechanism of ROS. The strong antibacterial performance observed in dark environments suggests that MgO has broad application prospects as an antibacterial agent.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 6","pages":"Article 119192"},"PeriodicalIF":7.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145097183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of acid mine drainage treatment process using arsenic adsorbing bacteria","authors":"Sohei Iwama ,&nbsp;Chikara Takano ,&nbsp;Satoru Kawasaki ,&nbsp;Kazunori Nakashima","doi":"10.1016/j.jece.2025.119222","DOIUrl":"10.1016/j.jece.2025.119222","url":null,"abstract":"<div><div>The excavation of mining sites supports technological advancements and material development by supplying valuable metals. However, this process results in the generation of acid mine drainage (AMD) containing harmful metals. Current neutralization treatments discharge harmful neutralized sludge. In this context, we previously proposed a novel bioprocess and isolated <em>Paenarthrobacter</em> sp. strain H1 with the ability to remove As from AMD (pH 1.95). In this study, the As removal mechanism of this bacterium was investigated, and a laboratory-scale AMD treatment process was developed. The mechanism was clarified based on dead cell As removal ability and cell surface observations. The results indicated that the bacterium adsorbed Fe(III) on the bacterial cell surface, and As(V) was adsorbed on Fe(III). This As adsorption behavior followed the Langmuir isotherm model. Using this bacterium, we established a three-cycle repeated As removal process that effectively reduced the As concentration in AMD from 7.2 to below 0.2 mg/L. Compared to the conventional neutralization process, this process reduces the amount and As concentration of the resulting neutralized sludge. Furthermore, the resulting sludge contained P, an essential plant nutrient, rendering it a potential agricultural fertilizer. Thus, the process using bacterial strain H1 possesses the potential to remove As from AMD and facilitate the restoration of mining sites. Although further studies are required, this study contributes to the establishment of a sustainable AMD treatment process with a low environmental impact and reduces the disposal of harmful sludge.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 6","pages":"Article 119222"},"PeriodicalIF":7.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crystallizing bacterial extracellular protein reveals paths of silver mineralization for recovery","authors":"Fiaz Ahmad ,&nbsp;Noreen Ashraf ,&nbsp;Xudong Deng,&nbsp;Da-Chuan Yin","doi":"10.1016/j.jece.2025.119215","DOIUrl":"10.1016/j.jece.2025.119215","url":null,"abstract":"<div><div>Heavy metal pollution calls for green recovery strategies. In response to metal stress, bacteria secrete extracellular proteins (ECPs), but the specific role of an individual extracellular protein (ECP) in silver ion (Ag²⁺) mineralization and recovery remains unexplored. Here, we investigated how a single ECP from silver-hypertolerant <em>Enterobacter cloacae</em> mediates Ag²⁺ mineralization. Proteomics (LC-MS/MS, MALDI-TOF) identified the 15.6 kDa protein as an inosine-monophosphate dehydrogenase (ImpD) homolog, whose secretion peaks in medium containing 15.9 ppm Ag²⁺. Partially purified ImpD crystallized at 20–30 °C. Time-resolved in-situ crystallography and X-ray diffraction captured monomers assembling into donut-shaped hexamers that weave into thread-like lattices; these ordered scaffolds template orientation-specific nucleation of Ag-rich crystals, enabling complete silver recovery from aqueous medium in 1.5 h. This previously unrecognized single-protein mediated biomineralization mechanism reveals specific ECPs as programmable bio-lixiviants, offering a low-energy, solvent-free route to metal recovery and expanding the toolkit for biometallurgy and environmental remediation.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 6","pages":"Article 119215"},"PeriodicalIF":7.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A critical review of biochar-enhanced Feammox: Multilevel mechanisms and sustainable applications for nitrogen removal","authors":"Luomiao Ji,&nbsp;Xiaonong Zhang,&nbsp;Rui Zhao,&nbsp;Xurui Zhu,&nbsp;Bo Gao,&nbsp;Peng Wu","doi":"10.1016/j.jece.2025.119240","DOIUrl":"10.1016/j.jece.2025.119240","url":null,"abstract":"<div><div>As a novel anaerobic nitrogen removal pathway, ammonium oxidation coupled with iron reduction (Feammox) demonstrates significant advantages in low C/N ratio environments. However, its practical application is limited by bottlenecks such as low electron transfer efficiency, unsustainable iron cycling, and pH sensitivity. Biochar provides an innovative solution for enhancing Feammox due to its unique porous structure, redox activity, and surface functional groups. This review systematically elucidates the quadruple mechanisms of biochar in enhancing Feammox performance: (i) serving as an electron shuttle to accelerate electron transfer between microbes and Fe(III); (ii) facilitating Fe(III)/Fe(II) cycling regeneration to address the challenge of sustained iron supply; (iii) optimizing microbial community structure by enriching Feammox bacteria and activating the expression of electron transfer-related genes; and (iv) regulating microenvironmental pH and extracellular polymeric substance (EPS) secretion to improve system resilience against environmental stressors. Key factors influencing biochar efficacy are summarized to guide the design of tailored biochar materials for specific Feammox applications. Furthermore, we propose a biochar-driven Feammox-Anammox-NDFO coupled process to achieve synchronous nitrogen-iron-electron cycling and NH₄⁺ /NO₃^- co-removal, offering a novel paradigm for low-carbon wastewater treatment. Future research should focus on the targeted design and optimization of biochar to achieve highly efficient Feammox enhancement, thereby advancing global nitrogen cycling and sustainable wastewater nitrogen removal technologies.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 6","pages":"Article 119240"},"PeriodicalIF":7.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carboxylated graphene quantum dots as emerging precursors of disinfection byproducts: Mechanistic insights into chlorine-driven transformation and environmental risk amplification","authors":"Min Zhang,&nbsp;Shasha Zhao,&nbsp;Xuhua Cheng,&nbsp;Manhong Miao,&nbsp;Xuantong Zheng,&nbsp;Yao Li","doi":"10.1016/j.jece.2025.119228","DOIUrl":"10.1016/j.jece.2025.119228","url":null,"abstract":"<div><div>Carboxylated graphene quantum dots (cGQDs), emerging from diverse industrial applications, pose significant environmental risks as precursors of disinfection byproducts (DBPs) in water treatment. This study systematically investigates the transformation mechanisms and DBPs formation potential of cGQDs during chlorination and chloramination. cGQDs undergo disruption of π-conjugated structures and covalent halogenation through direct chlorination and indirect radical-mediated (•OH, Cl•, ClO•), resulting in substantial DBPs generation. Notably, trichloromethane (TCM) concentrations reached 146.47 µg/L (low chlorine) and 697.44 µg/L (high chlorine). Particularly under low chlorine conditions, which represent concentrations typical of municipal wastewater disinfection, cGQDs produced significantly higher TCM than conventional carbon materials at equivalent concentrations, exceeding those of graphene oxide and graphene by 3-fold and 29-fold, respectively. This enhanced reactivity is attributed to nanoscale dimensions and carboxyl-rich surfaces of cGQDs. In contrast, although chloramination can reduce the generation of DBPs, it may lead to more severe environmental impacts, such as the formation of nitrogen-doped GQDs (N-GQDs) with a narrower bandgap, which can complex with metal ions like iron(III) and subsequently affect water quality. Real-water experiments demonstrated that while natural organic matter partially suppresses DBPs formation from cGQDs via radical scavenging, cGQDs still increased TCM formation by 20.8 % in surface water and 21.3 % in wastewater. These findings highlight the unique reactivity of cGQDs as DBPs precursors, thereby providing critical insights for refining disinfection strategies and managing nanomaterial-related hazards in water treatment.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 6","pages":"Article 119228"},"PeriodicalIF":7.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ball-milled biochar: Structural transformation and implications for Cu and Pb immobilization in smelter-contaminated soil","authors":"Mariusz Gusiatin ,&nbsp;Mirosław Bramowicz ,&nbsp;Bartosz Pszczółkowski ,&nbsp;Sławomir Kulesza ,&nbsp;Monika Gwoździk ,&nbsp;Anna Gawryszuk-Rżysko","doi":"10.1016/j.jece.2025.119225","DOIUrl":"10.1016/j.jece.2025.119225","url":null,"abstract":"<div><div>Ball-milled biochar (BBC) was evaluated for long-term immobilization of copper (Cu) and lead (Pb) in smelter-contaminated soil. In a one-year column leaching test, soil amended with 5 % (w/w) BBC was compared with macro-sized biochar (MBC). Biochars were characterized by scanning electron microscopy, Brunauer–Emmett–Teller surface area, X-ray diffraction, Fourier transform infrared spectroscopy, 3D topography, and Morphologi G3 imaging. Leachates were periodically collected for 356-day and analyzed for heavy metals (HMs) and leachate chemistry. Ball-milling increased biochar reactivity by raising surface area six-fold, introducing microporosity, and enriching oxygen-containing functional groups. BBC particles were 63–66 % smaller, more fragmented, and showed greater surface roughness and crystallinity than MBC. The largest change in leachate chemistry pH, electrical conductivity, water-soluble organic carbon and its spectral indices occurred during the first 50 days, coinciding with the highest HM release. Leaching kinetics followed a first-order model, with lower HM release rate constants in BBC-amended columns. BBC also showed better efficiency of HM immobilization: Cu and Pb leaching reduction was 57.6 % and 22.9 %, Cu and Pb reduction in mobile fractions was 19.0 % and 24.2 %, and Cu and Pb increase in stable fractions was 30.2 % and 10.0 %, respectively. Using nine soil indicators, the soil quality index was highest for BBC-amended soil. These findings demonstrate greater long-term effectiveness of BBC than MBC in immobilizing Cu and Pb. They also provide evidence linking biochar microstructure to HM leaching kinetics, effluent chemistry, and soil quality, supporting BBC as a practical and sustainable amendment for smelter-contaminated soils.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 6","pages":"Article 119225"},"PeriodicalIF":7.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SMFC-driven disruption of iron-sulfur redox coupling as a bioelectrochemical barrier against endogenous phosphorus release in eutrophic lake Taihu, China","authors":"Hang Qi ,&nbsp;Xinyu Lu ,&nbsp;Xianglong Zhang ,&nbsp;Wei Jin","doi":"10.1016/j.jece.2025.119176","DOIUrl":"10.1016/j.jece.2025.119176","url":null,"abstract":"<div><div>Legacy sedimentary phosphorus undergoes microbially mediated reactivation, perpetuating harmful algal blooms. Sediment microbial fuel cells (SMFCs) suppress phosphorus liberation through targeted manipulation of sulfur-driven iron reduction, whereas their integrated impacts on biogeochemical cycling and microbiome dynamics in complex eutrophic environments require systematic elucidation. In this study, a two-chamber SMFC system was constructed to investigate the electrochemical regulation of phosphorus (P), iron (Fe), and sulfur (S) cycling dynamics. Phosphorus transfer-transformation mechanisms were elucidated through cross-interface physicochemical characterization of anode sediment-overlying water interfaces, continuous voltage monitoring, and metagenomic community profiling. The results showed that the closed-circuit SMFC significantly altered sediment pH compared to control, with gradual decreases at deeper depths. This pH change correlated with a 71 % reduction in overlying water total phosphorus. Concurrently, a 21.46 % SO₄^2- increase in deep pore water (−6 cm) confirmed enhanced sulfur oxidation, suppressing PO₄^3- release. Solid-phase analysis revealed a 7.09 % reduction in NaOH-P (metal-bound P) at mid-depth (−3 cm). An accompanying BD-P (Fe-bound P) increase confirmed slowed iron reduction, inhibiting phosphate release. The relative abundances of <em>Pseudomonadota</em> and <em>Chlorobi</em>, to which sulfur-oxidizing bacteria belong, were 23.06 % and 18.35 %, respectively, which were significantly higher than those of the control group, indicating that the SMFC has a significant enrichment effect on specific functional microbial communities. This study reveals that SMFCs alter sediment redox dynamics by accelerating sulfur oxidation while suppressing iron reduction, thereby inhibiting phosphorus release. These mechanistic advances deepen understanding of phosphorus biogeochemistry in disturbed sediments, offering scientific basis for future global eutrophication control strategies.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 6","pages":"Article 119176"},"PeriodicalIF":7.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioelectrochemical conversion of CO2 to valuable chemicals through microbial electrosynthesis: State-of-the-art for current progress on green strategies with circular economy nexus","authors":"Swati Das ,&nbsp;Sovik Das ,&nbsp;Makarand M. Ghangrekar ,&nbsp;Booki Min","doi":"10.1016/j.jece.2025.119221","DOIUrl":"10.1016/j.jece.2025.119221","url":null,"abstract":"<div><div>The rising global concerns over climate change and the urgent need for sustainable energy have accelerated research into cutting-edge technologies for converting CO₂ to valuable compounds. One promising approach involves microbial electrosynthesis (MES) for converting CO₂ into biofuels and platform chemicals, utilising electrotrophic microorganisms as biocatalysts. Nevertheless, low productivity of value-added chemicals and non-optimised MES configurations, including substrate limitation, are noteworthy bottlenecks that hinder the scalability of this technology. To overcome these limitations, researchers have explored different reactor designs and electrode modifications with biotic and abiotic catalysts to improve the interaction between materials and microbes, ultimately leading to increased product yield. Hence, this review emphasises the insights of MES in the context of reactor configurations and the modification of electrode material via nature-based strategy with life cycle assessments for driving a circular carbon economy. Besides, different genetic and synthetic biological processes, integration of quorum sensing, 3D printing, and machine learning as novel and promising approaches, along with challenges and future perspectives to advance MES, are highlighted in the present review, which has not been critically reviewed to date. Thus, this review attempts to present a holistic assessment of the applicability of MES that intends to guide researchers in assessing the feasibility of this technology by integrating inexpensive and non-energy-intensive green strategies.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 6","pages":"Article 119221"},"PeriodicalIF":7.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145097579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significantly enhanced piezo-photocatalytic in-situ H2O2 generation and activation for efficient antibiotic degradation by g-C3N4/NH2-MIL-101(Fe) heterojunction","authors":"Yao Zhang ,&nbsp;Yong Jiang ,&nbsp;Wenbo Fu ,&nbsp;Chunyang Zhai ,&nbsp;Jing Guo","doi":"10.1016/j.jece.2025.119236","DOIUrl":"10.1016/j.jece.2025.119236","url":null,"abstract":"<div><div>This study demonstrates the synergistic effect of piezocatalysis and photocatalysis can effectively promote the generation and in-<em>situ</em> activation of hydrogen peroxide (H₂O₂) and enhance the degradation performance of antibiotics. A novel g-C₃N₄/NH₂-MIL-101(Fe) (g-C₃N₄/NMOF) Z-scheme heterojunction was prepared via solvothermal approach. The piezoelectricity of g-C₃N₄/NMOF improves the separation efficiency and lifetime of photogenerated carriers, which is beneficial to the generation of H₂O₂ and degradation of ciprofloxacin (CIP) in piezo-photocatalytic system. Under piezo-photocatalytic progress, g-C₃N₄/NMOF achieves 0.1102 mM/h H₂O₂ yield in pure water, which is 20.6 times and 2.96 times higher than g-C₃N₄/NMOF in piezocatalysis and photocatalysis progresses, respectively. Mechanistic studies reveal that the ·O₂^- is the main intermediate product of H₂O₂, and the generation of H₂O₂ occurs through a two-step one-electron pathway (O₂ → ·O₂^- → H₂O₂). Moreover, g-C₃N₄/NMOF with abundant Fe²⁺ could directly activate H₂O₂ to generate hydroxyl radicals (·OH), forming an in-<em>situ</em> Fenton system and significantly accelerating CIP degradation. The degradation efficiency of CIP reaches 89.87 % within 60 min under piezo-photocatalytic condition, which is 3.25 and 2.54 folds higher than that alone condition. The piezo-photocatalytic H₂O₂ generation and in-<em>situ</em> Fenton mechanism of co-utilizing solar and mechanical energy by g-C₃N₄/NMOF may have potential in applications for cost-effective environmental remediation.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 6","pages":"Article 119236"},"PeriodicalIF":7.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145097634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated optimization and environmental application of chlorohydrolase-positive bacterial isolates for enhanced atrazine degradation and genotoxicity assessment","authors":"Simranjeet Singh ,&nbsp;Vijay Kumar ,&nbsp;Shivika Datta ,&nbsp;Kaushal Sood ,&nbsp;Yashika Gandhi ,&nbsp;Nadeem A. Khan ,&nbsp;Harry Kaur ,&nbsp;Joginder Singh ,&nbsp;Praveen C. Ramamurthy ,&nbsp;Sami Rtimi","doi":"10.1016/j.jece.2025.119234","DOIUrl":"10.1016/j.jece.2025.119234","url":null,"abstract":"<div><div>Atrazine (ATZ), a widely used broad-leaved weed management chemical in agriculture, with a half-life of 30–740 days, poses a threat to the ecosystem. Its residue is found in various levels of soil and aquatic ecosystems. In this study, three new strains were identified and used to treat effectively, thereby reducing toxicity and rapid degradation. Bacillus <em>mycoides</em> strain (P2), <em>Pseudomonas sp. strain</em>(P3), and <em>Pseudomonas furukawaii</em> (P6). The degradation efficiency was found to follow the order P3 &gt; P2 &gt; P6. Over a twelve-day experiment, all strains demonstrated atrazine utilization, with a half-life period ranging from 65.53 to 102.32 days by using pseudo-first-order kinetics. Response surface methodology was employed to optimize the biodegradation parameters, including atrazine dosage, pH, and temperature, for all three strains. The investigation revealed that the optimum conditions for atrazine degradation by all strains were pH 7.4, temperature 32 ± 2 °C, and concentration 100 mg/L. Gas chromatography-mass spectrometry results identified the major degradation products of atrazine, including Deisopropylatrazine (DIA), Deethylatrazine (DEA), and Cyanuric acid (CNA) at RT 6.46, 12.22, and 14.22, respectively, also closely align with the predicted statistical design model. All three isolated strains exhibited hydrolysis of Atrazine, suggesting the presence of intracellular Atrazine chlorohydrolase enzymes. Genotoxicity studies on the degradation products depicted toxicity in the order Atrazine&gt;DEA&gt;DIA&gt;CNA. These atrazine-biodegrading isolates show promise as effective remediators for atrazine-contaminated agricultural soils and crop fields in terms of laboratory efficacy and practical application, as well as for environmental cleaning processes involving other pesticides.</div></div>","PeriodicalId":15759,"journal":{"name":"Journal of Environmental Chemical Engineering","volume":"13 6","pages":"Article 119234"},"PeriodicalIF":7.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145061004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}