Eric Oriol, Beatriz Schettino, Gustavo Viniegra-Gonzales, Maurice Raimbault
{"title":"Solid-state culture of Aspergillus niger on support","authors":"Eric Oriol, Beatriz Schettino, Gustavo Viniegra-Gonzales, Maurice Raimbault","doi":"10.1016/0385-6380(88)90130-6","DOIUrl":"10.1016/0385-6380(88)90130-6","url":null,"abstract":"<div><p>The growth kinetics of <em>Aspergillus niger</em> on a solid support <em>i.e.</em> sugarcane bagasse, impregnated with a liquid glucose medium, were investigated under different culture conditions. The water activity of the medium, the amount of spore inoculum and the support particle size were shown to be critical factors for mold growth. The elevated rates of growth observed with high substrate concentration media demonstrated the feasibility of the method for culturing filamentous fungi.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 1","pages":"Pages 57-62"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90130-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83222437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A novel process of ethanol production accompanied by extraction of sugar in cane chips","authors":"Susumu Fukushima, Kazuhiro Yamade","doi":"10.1016/0385-6380(88)90009-X","DOIUrl":"10.1016/0385-6380(88)90009-X","url":null,"abstract":"<div><p>By connecting a rhomboid unit for fermentation and an exchangeable tubular unit for extraction, a novel bioreactor was designed to produce high concentration of ethanol solution from non-peeled sugar cane chips by means of a cyclic system for exchanging old chips with new ones. The rhomboid and tubular units were packed with 1.0–1.5 mm biocatalyst entrapped yeast-cells with Al alginateand cane chips of 2.0–3.0 mm in width, respectively. The volume ratio of the two units was 1.0. At the start of the first cycle, 2 g/<em>l</em> of Al<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> 14–18 H<sub>2</sub>O solution was added to the bioreactor, where the ratio of the solution to the working volume was 0.85. Both sugar extraction and fermentation were anaerobically performed at pH 2.5 and a temperature of 30°C by circulating the solution through the two units. After each cycle, the tubular unit was exchanged for a new unit packed with new chips. The combined solution of free ethanol and the ethanol obtained by pressing the old chips was re-used as the circulating solution.</p><p>When the volume ratio of the biocatalyst to the total working volume was 0.1 and the amount of dried cane chips in the tubular unit was 200 g/<em>l</em>, 16% (w/v) ethanol was produced after 7 cycles. Each cycle was established at about 20 h. The number of free cells in the circulating solution was only 2×10<sup>7</sup>/ml after 7 cycles.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 4","pages":"Pages 423-426"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90009-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86580398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Flocculation of Aspergillus terreus with polyelectrolyte complex and production of itaconic acid with the flocculated mycelia","authors":"Etsuo Kokufuta, Shin Suzuki, Isei Nakamura","doi":"10.1016/0385-6380(88)90011-8","DOIUrl":"10.1016/0385-6380(88)90011-8","url":null,"abstract":"<div><p>Mycelia from <em>Aspergillus terreus</em> K 26 were flocculated with a polyelectrolyte complex consisting of potassium poly9vinyl alcohol) sulfate (KPVS) and poly(diallyldimethyl-ammonium chloride) (PDDA) by three different methods: (a) PDDA was added into the broth obtained from precultivation of the hyphal inoculum in the presence of KPVS; (b) use of KPVS and PDDA was reversed from that in method a; (c) after the precultivation in the absence of the polymer, the mycelia were harvested, dispersed in 0.1 M phosphate buffer containing PDDA, then flocculated by addition of KPVS. The three types of the flocculated mycelia were investigated concerning growth and itaconic acid production in shake flask cultures. Viscosity and sedimentation were further examined to characterize the flocculated mycelial broths. A slight inhibition caused by flocculation on growth and acid production was observed at the beginning of repeated cultivation, but this was eliminated when cultivation in the fresh medium was repeated. There was no marked difference in the specific rates of acid production between fre and flocculated cells. Viscosity of the flocculated mycelial system was close to that of the medium, even while maintaining a cell concentration of 2 g/dl. The poor sedimentation of mycelia was favorably imporved with these flocculation methods, especially with methods b and c.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 4","pages":"Pages 433-439"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90011-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86194173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation and cultural conditions of cyclohexylacetic acid using bacterium","authors":"Fumiki Yoshizako, Mitsuo Chubachi, Atsuo Nishimura, Teruo Ueno","doi":"10.1016/0385-6380(88)90142-2","DOIUrl":"10.1016/0385-6380(88)90142-2","url":null,"abstract":"","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 1","pages":"Pages 127-128"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90142-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77415456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SCP production and application of nitrogen-fixing ability of Azotobacter sp. grown on ethanol","authors":"Toshio Omori, Ichie Nishibori, Hiromi Abe, Kayoko Tsuchiya, Tohru Kodama","doi":"10.1016/0385-6380(88)90056-8","DOIUrl":"10.1016/0385-6380(88)90056-8","url":null,"abstract":"","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 2","pages":"Page 243"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90056-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74068208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ethanol production from wheat flour by Zymomonas mobilis","authors":"E. Favela Torres, J. Baratti","doi":"10.1016/0385-6380(88)90043-X","DOIUrl":"10.1016/0385-6380(88)90043-X","url":null,"abstract":"<div><p>Starch from wheat flour was enzymatically hydrolyzed and used for ethanol production by <em>Zymmonas mobilis</em>. The addition of a nitrogen source like ammonium sulfate was sufficient to obtain a complete fermentation of the hdyrolyzed strach. In batch culture a glucose concentration as high as 223 g/<em>l</em> could be fermented (conversion 99.5%) to 105 g/<em>l</em> of ethanol in 70 h with an ethanol yield of 0.47 g/g (92% of theoretical). In continuous culture the use of a flocculent strain and a fermentor with an internal settler resulted (D=1,4 h<sup>−1</sup>) in a high ethanol productivity of 70.7 g/<em>l</em>·h with: ethanol concentration 49.5 g/<em>l</em>, ethanol yield 0.50 g/g (98% of theoretical and substrate conversion 99%.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 2","pages":"Pages 167-172"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90043-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75861855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hae Sung Jee, Takumi Mano, Naomichi Nishio, Shiro Nagai
{"title":"Influence of redox potential on methanation of methanol by Methanosarcina barkeri in Eh-stat batch cultures","authors":"Hae Sung Jee, Takumi Mano, Naomichi Nishio, Shiro Nagai","doi":"10.1016/0385-6380(88)90138-0","DOIUrl":"10.1016/0385-6380(88)90138-0","url":null,"abstract":"<div><p>The effect of Eh on the methanogenesis of methanol by <em>Methanosarcina barkeri</em> strain Fusaro was studied in pH-controlled anaerobic batch cultures at 37°C, in which the Eh of the culture medium was controlled by the addition of Ti(III)-citrate at values ranging from −340 to −520 mV. The changes in Eh revealed that the specific growth rate, μ, specific methane production rate, <em>Q</em><sub>CH<sub>4</sub></sub> and growth yield, <em>Y</em><sub>X/S</sub> were optimum under an Eh between −430 and −520 mV, while they decreased at the higher Eh of −340 mV. The maximum values of <em>Q</em><sub>CH<sub>4</sub></sub> and μ under the optimum Eh condition were 210 ml CH<sub>4</sub>/g dry cell weight·h<sup>−1</sup> and 0.11 h<sup>−1</sup>, respectively.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 1","pages":"Pages 123-126"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90138-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74825119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Purification and properties of glutaminase from Aspergillus oryzae","authors":"Toshihiro Yano, Masae Ito, Kenji Tomita, Hidehiko Kumagai","doi":"10.1016/0385-6380(88)90039-8","DOIUrl":"10.1016/0385-6380(88)90039-8","url":null,"abstract":"<div><p>Glutaminase activity was found in a water extract of a wheat bran <em>koji</em> (extracellular fraction) of <em>Aspergillus oryzae</em> strains Lee-1, H-16 and MA-27-IM isolated from a commercial <em>koji</em> ssed for soy sauce fermentation, as well as in thier mycelia (intracellular fraction). Both the intracellular and the extracellular glutaminases were purified from strain MA-27-IM. Polyacrylamide gel electrophoresis of each purified preparation gave a single band with identical electrophoretic mobility. The molecular weight of the intracellular and the extracellular glutaminases were estimated to be approximately 113, 000. Both preparations hydrolyzed various γ-glutamyl compounds besides <span>l</span>-glutamine but did not exhibit asparaginase activity. Further investigation of these preparations inidicated that these glutaminases possessed almost the same properties, suggesting their similarity.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 2","pages":"Pages 137-143"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90039-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73213166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Production of raw cassava starch-digestive glucoamylase by Rhizopus sp. in liquid culture","authors":"Hiroshi Nishise , Akira Fuji, Makoto Ueno, Vitchuporn Vongsuvanlert, Yoshiki Tani","doi":"10.1016/0385-6380(88)90005-2","DOIUrl":"10.1016/0385-6380(88)90005-2","url":null,"abstract":"<div><p>Among about 200 <em>Rhizopus</em> strains isolated in Thailand, <em>Rhizopus</em> sp. MB46 was selected as a producer of raw cassava starch-digestive glucoamylase. Rice bran was effective for the enzyme production in a solid culture as well as wheat bran. Addition of turpentine oil into the rice bran solid culture increased the productivity. <em>Rhizopus</em> sp. MB46 was found to produce glucoamylase in a liquid culture containing 1% rice bran but not in one consisting of 10% raw cassava starch of 2% glucose. The productivity per 1 g solids in the medium in liquid culture was finally improved 6-times by utilization of <em>n</em>-hexane-treated rice bran, supplement of 0.1% meat extract and addition of gauze as a support. The activity was superior to that in turpentine oil-supplemented solid culture.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 4","pages":"Pages 397-402"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90005-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85973503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}