Journal of electron microscopy technique最新文献_第3页

An abbreviated method for preparing Ixodes damini ticks for scanning electron microscopy observation. 一种制备扫描电子显微镜观察用大角蜱虫的简易方法。

Journal of electron microscopy technique Pub Date : 1990-05-01 DOI: 10.1002/jemt.1060150110

M B Rupp

引用次数: 9

Composite replicas: methodologies for direct evaluation of the relationship between intramembrane and extramembrane structures. 复合复制品:直接评估膜内和膜外结构之间关系的方法。

Journal of electron microscopy technique Pub Date : 1989-11-01 DOI: 10.1002/jemt.1060130308

R A Coleman, J B Wade

{"title":"Composite replicas: methodologies for direct evaluation of the relationship between intramembrane and extramembrane structures.","authors":"R A Coleman, J B Wade","doi":"10.1002/jemt.1060130308","DOIUrl":"https://doi.org/10.1002/jemt.1060130308","url":null,"abstract":"Electron microscopic studies of membrane structure have been facilitated by the recent development of the composite replica technique in which the membrane is freeze-fractured, then inverted and the surface deep-etched and replicated. Examination in stereo of this composite preparation of two replicas with interposed half-membrane and associated surface elements reveals the physical relationship between structures on the surface and within the membrane. Composite replicas of the toad urinary bladder surface demonstrated connections of filamentous glycocalyx elements to intramembrane particles (IMPs). Using a bidirectional shadowing technique, many membrane surface particles also are shown to be associated with underlying IMPs, suggesting that these membrane surface particles are projections of the IMPs above the surface of the membrane. There is evidence that elements whose attachment sites relate to the half-membrane fractured away can be displaced from the membrane surface and lost. Labelling studies using colloidal gold-labelled antibodies were carried out to assess loss of surface mesh from fractured membrane. Gold distributions and amounts were similar in labelled surface replicas, label-fracture specimens, and labelled composite replicas, yet the amount of mesh detected in the composite replicas was less than in the surface replicas. This suggests that while some unlabelled or lightly labelled surface elements can be lost from fractured membranes, ligands stabilize elements and reduce their loss apparently by cross-linking them.","PeriodicalId":15690,"journal":{"name":"Journal of electron microscopy technique","volume":"13 3","pages":"216-27"},"PeriodicalIF":0.0,"publicationDate":"1989-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jemt.1060130308","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13655847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Quick-freeze, deep-etch, rotary-shadow views of the extracellular matrix and cortical cytoskeleton of Xenopus laevis eggs. 非洲爪蟾卵的细胞外基质和皮质细胞骨架的速冻，深蚀刻，旋转阴影视图。

Journal of electron microscopy technique Pub Date : 1989-11-01 DOI: 10.1002/jemt.1060130309

C A Larabell, D E Chandler

引用次数: 7

A method for obtaining light microscopic survey sections from fresh frozen tissue during cryoultramicrotomy. 一种在冷冻显微切开术中从新鲜冷冻组织获得光显微切片的方法。

Journal of electron microscopy technique Pub Date : 1989-11-01 DOI: 10.1002/jemt.1060130311

K E Tvedt, J Halgunset, G Kopstad, O A Haugen

引用次数: 0

Flat mold embedding with LR white and Lowicryl K4M. 用LR白和低丙基K4M平模包埋。

Journal of electron microscopy technique Pub Date : 1989-11-01 DOI: 10.1002/jemt.1060130312

A Simpson-Gomes, G T Simon

引用次数: 4

Improved shielding for energy-dispersive X-ray microanalysis in the high-voltage electron microscope. 高压电子显微镜下能量色散x射线微分析的改进屏蔽。

Journal of electron microscopy technique Pub Date : 1989-11-01 DOI: 10.1002/jemt.1060130316

W F Tivol

引用次数: 0

High-pressure freezing for the preservation of biological structure: theory and practice. 高压冷冻保存生物结构:理论与实践。

Journal of electron microscopy technique Pub Date : 1989-11-01 DOI: 10.1002/jemt.1060130305

R Dahl, L A Staehelin

引用次数: 300

Freeze-fracture cytochemistry: review of methods. 冻裂细胞化学:方法综述。

Journal of electron microscopy technique Pub Date : 1989-11-01 DOI: 10.1002/jemt.1060130306

N J Severs

{"title":"Freeze-fracture cytochemistry: review of methods.","authors":"N J Severs","doi":"10.1002/jemt.1060130306","DOIUrl":"https://doi.org/10.1002/jemt.1060130306","url":null,"abstract":"\"Freeze-fracture cytochemistry\" encompasses a diversity of recently developed techniques in which freeze-fracture and cytochemistry are combined. Cytochemical labeling may, in principle, be integrated into one of three basic points in the standard freeze-fracture procedure; 1) before the specimen is frozen, 2) after it has been fractured, or 3) after it has been platinum shadowed and/or carbon coated. Visualization of the labeled cellular structures can be achieved by a variety of different methodologies. For example, the markers (usually colloidal gold particles) may be viewed embedded within a replica, or attached to it via fragments of membrane (or other cellular components). Sectioning is a central strategy in a number of techniques, either in combination with or in place of replication. The different combinations of methods that have been devised are not, for the most part, alternative ways of arriving at the same result; each provides quite distinct information about specific classes of membrane component or other structure in the cell. The purpose of this review is to present, within a single article, a systematic survey of the full range of techniques currently available in freeze-fracture cytochemistry. Emphasis is placed on explaining the principles underlying the methods and on illustrating their applications. With the success recently achieved, freeze-fracture cytochemistry has moved from the phase of experimental development to a position in which it may be expected increasingly to make significant contributions across a wide spectrum of problems in cell and membrane biology.","PeriodicalId":15690,"journal":{"name":"Journal of electron microscopy technique","volume":"13 3","pages":"175-203"},"PeriodicalIF":0.0,"publicationDate":"1989-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/jemt.1060130306","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13826960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Protocol for 3-D visualization of molecules on mica via the quick-freeze, deep-etch technique. 通过速冻深蚀刻技术对云母分子进行三维可视化的方案。

Journal of electron microscopy technique Pub Date : 1989-11-01 DOI: 10.1002/jemt.1060130310

J Heuser

引用次数: 94

Current trends in freeze-fracture, part I. 冻结断裂的当前趋势，第一部分。

Journal of electron microscopy technique Pub Date : 1989-11-01 DOI: 10.1002/jemt.1060130303

引用次数: 0