Journal of Environmental Science and Health, Part B最新文献

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Adsorption of chlortetracycline in aquaculture wastewater by lanthanum modified multi-walled carbon nanotubes 镧修饰多壁碳纳米管对水产养殖废水中氯霉素的吸附研究
Journal of Environmental Science and Health, Part B Pub Date : 2022-05-03 DOI: 10.1080/03601234.2022.2061261
Yuqi Zhang, Xiaocai Yu, Yifu Liu, Shini Wu, Runqiang Yu, Tao Chen
{"title":"Adsorption of chlortetracycline in aquaculture wastewater by lanthanum modified multi-walled carbon nanotubes","authors":"Yuqi Zhang, Xiaocai Yu, Yifu Liu, Shini Wu, Runqiang Yu, Tao Chen","doi":"10.1080/03601234.2022.2061261","DOIUrl":"https://doi.org/10.1080/03601234.2022.2061261","url":null,"abstract":"Abstract The lanthanum modified multi-walled carbon nanotubes (La-CNTs) prepared by an impregnation method were investigated for the adsorption of chlortetracycline (CTC) in aquaculture wastewater. The adsorbents were characterized by SEM, EDS, XRD and BET. The effects of some factors including La-containing impregnant concentration, adsorbent dosage, CTC adsorbate concentration, adsorption time, pH of the adsorbate solution and additional ions on the CTC adsorption by La-CNTs were investigated in detail, and the optimal adsorption conditions were determined. The adsorption kinetics obeyed the quasi-second-order kinetic model. The adsorption isotherms obeyed the Langmuir model and the fitted maximum capacity of La-CNTs for CTC adsorption was 55.3 mg/g.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"28 1","pages":"369 - 378"},"PeriodicalIF":0.0,"publicationDate":"2022-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88112909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Development of a colloidal gold immunochromatographic strip for the rapid detection of pefloxacin in grass carp with a novel pretreatment method 建立一种新型前处理方法快速检测草鱼中培氟沙星的胶体金免疫层析试纸条
Journal of Environmental Science and Health, Part B Pub Date : 2022-04-27 DOI: 10.1080/03601234.2022.2068908
Xinghua Zhou, Na Li, Chenjun Sun, Xun Zhang, C. Zhang, Jiayu Zhou, Shuoning Guan, Xiang Xiao, Yun Wang
{"title":"Development of a colloidal gold immunochromatographic strip for the rapid detection of pefloxacin in grass carp with a novel pretreatment method","authors":"Xinghua Zhou, Na Li, Chenjun Sun, Xun Zhang, C. Zhang, Jiayu Zhou, Shuoning Guan, Xiang Xiao, Yun Wang","doi":"10.1080/03601234.2022.2068908","DOIUrl":"https://doi.org/10.1080/03601234.2022.2068908","url":null,"abstract":"Abstract A rapid colloidal gold immunochromatography assay (GICA) for the detection of pefloxacin (PEF) was established and optimized. The anti-PEF monoclonal antibody (mAb) was used to target PEF as a colloidal gold-mAb conjugate. The mAb belonged to the IgG2b subtype, lambda light chain, the affinity constant (Ka) was 5.21 × 109 L·mol−1, and its half maximal inhibitory concentration (IC50) was 0.23 ng·mL−1. No obvious cross-reactivity (CR) was observed with other common fluoroquinolone antibiotics, including ciprofloxacin (CIP), norfloxacin (NOR), lomefloxacin (LOM) and ofloxacin (OFL). The visual limit of detection (vLOD) of the optimized GICA was 2 ng·g−1 under the conventional pretreatment method, and the assay was completed in 15 min. Liquid chromatography tandem-mass spectrometry (LC–MS/MS) was employed to confirm the performance of the strip. In addition, a novel pretreatment was established and compared with conventional pretreatment. Without the removal of organic solvents, the novel pretreatment method reduced the sample pretreatment time (more than 10 min). The vLOD of the optimized GICA was also 2 ng·g−1 when applying the novel pretreatment method. In conclusion, the proposed PEF-GICA could detect samples containing PEF rapidly and accurately, and the novel pretreatment method saved the time of sample pretreatment and improved the efficiency of detection.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"34 1","pages":"517 - 525"},"PeriodicalIF":0.0,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76532079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rupture of Brumadinho dam (Minas Gerais, Brazil): embryotoxicity in zebrafish induced by metal mixture-contaminated water Brumadinho大坝(Minas Gerais,巴西)破裂:金属混合物污染水对斑马鱼的胚胎毒性
Journal of Environmental Science and Health, Part B Pub Date : 2022-04-27 DOI: 10.1080/03601234.2022.2064675
Paloma Vitória Lima Peixoto, Ítalo Bertoni Lopes de Andrade, Bianca Camargo Penteado Sales, L. C. Pereira
{"title":"Rupture of Brumadinho dam (Minas Gerais, Brazil): embryotoxicity in zebrafish induced by metal mixture-contaminated water","authors":"Paloma Vitória Lima Peixoto, Ítalo Bertoni Lopes de Andrade, Bianca Camargo Penteado Sales, L. C. Pereira","doi":"10.1080/03601234.2022.2064675","DOIUrl":"https://doi.org/10.1080/03601234.2022.2064675","url":null,"abstract":"Abstract Rupture of dam B-1 at the Córrego do Feijão mine in Brumadinho, state of Minas Gerais, Brazil (25/Jan/2019) contaminated the Paraopeba River with mine waste. To identify the adverse effects of this event on public and environmental health, we conducted the Fish Embryo Acute Toxicity (FET) test (OECD No. 236). During the tests, zebrafish embryos were exposed for 144 hours to solutions containing realistic concentrations of dissolved iron and aluminum and total manganese at the following analysis points upstream or downstream of the dam: 10 km, upstream; 19.7 km, downstream, at the point where water for consumption is collected; 24.5 km, downstream, in the city of Mário Franco; and 59 km, downstream, on the border between the towns of Juatuba and Betim. Metal concentrations were taken from September 2019 report No. 53 released by IGAM. Mortality was high at all exposure points and reached 93% at the Juatuba/Betim point. We also detected lethal, sublethal and teratogenic effects, such as non-hatching, non-inflation of the swim bladder, pericardial edema and scoliosis, affecting up to 25% of embryos at the other analysis points. The results highlight the need for continuous monitoring of the water quality of the Paraopeba River.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"40 1","pages":"479 - 488"},"PeriodicalIF":0.0,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82769331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Evaluation of myenteric neurons in the colon of rats exposed to 2,4 dichlorophenoxyacetic acid herbicide 2,4二氯苯氧乙酸除草剂对大鼠结肠肌内神经元的影响
Journal of Environmental Science and Health, Part B Pub Date : 2022-04-19 DOI: 10.1080/03601234.2022.2064674
Wagner Nanni, G. S. Porto, J. N. B. Pereira, Alexandre Rodrigo Nascimento Gonçalves, Gabriela Pustiglione Marinsek, S. R. Stabille, P. Favetta, R. M. Germano, R. B. Mari
{"title":"Evaluation of myenteric neurons in the colon of rats exposed to 2,4 dichlorophenoxyacetic acid herbicide","authors":"Wagner Nanni, G. S. Porto, J. N. B. Pereira, Alexandre Rodrigo Nascimento Gonçalves, Gabriela Pustiglione Marinsek, S. R. Stabille, P. Favetta, R. M. Germano, R. B. Mari","doi":"10.1080/03601234.2022.2064674","DOIUrl":"https://doi.org/10.1080/03601234.2022.2064674","url":null,"abstract":"Abstract The assessment of the enteric nervous system provides a better understanding of the effects that contaminants can have on the health and well-being of organisms. It has been reported that 2,4-dichlorophenoxyacetic acid (2,4-D) is a highly persistent herbicide in the environment that is responsible for neurotoxic changes in different myenteric neuronal subpopulations. The current study aimed to evaluate the effects of 2,4-D on myenteric neurons in the colon of Rattus norvegicus for the first time. A dose of 2,4-D (5 mg/kg/day) was administered to the experimental group (2,4-D) for 15 days. Then, the proximal colon was collected and submitted to Giemsa and NADPH-d histochemical techniques for the disclosure of total and nitrergic neurons. The 2,4-D group presented a higher density of total neurons (p = 0.05, t-test), which together with the maintenance of nitrergic neuronal density, may be related to the increase in the expression of the neurotransmitter acetylcholine by colocalization, responsible for stimulating the intestinal smooth muscle and increasing the chances of the expulsion of the harmful content present in the lumen. Over 15 days, the neurotoxic effects of 2,4-D in the myenteric plexus influenced an increase in the general population of myenteric neurons in the colon.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"58 1","pages":"421 - 429"},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89057081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Study on degradation characteristics of imazamox by Streptomycetaceae 链霉菌科对异丙唑啉的降解特性研究
Journal of Environmental Science and Health, Part B Pub Date : 2022-04-19 DOI: 10.1080/03601234.2022.2064673
Ling Ge, Xin Wang, Jianjiang Hou, Zijun Ni, Wen-Rui Liu, Jia Bao, Yulian Wei
{"title":"Study on degradation characteristics of imazamox by Streptomycetaceae","authors":"Ling Ge, Xin Wang, Jianjiang Hou, Zijun Ni, Wen-Rui Liu, Jia Bao, Yulian Wei","doi":"10.1080/03601234.2022.2064673","DOIUrl":"https://doi.org/10.1080/03601234.2022.2064673","url":null,"abstract":"Abstract The residues of imazamox (IMX) will cause phytotoxicity to subsequent crops after long-term use, and will also pollute the soil and its surrounding environment. This study isolates and identifies two strains of Streptomycetaceae JX02 and JX06 that can effectively degrade IMX. Use response surface method Box–Behnken design to optimize physicochemical parameters. The optimal degradation conditions of strains JX02 and JX06 are obtained and verified: IMX concentration is 150 mg L−1, the initial dosage is 9.9%, 9.1% (OD600 = 0.1), the temperature is 26.4 and 27.5 °C, and pH value is 7.0 and 7.7, respectively. The degradation rates of 150 mg L−1 IMX detected by HPLC within 4 d were 99 and 94%, respectively. After adding strains JX02 and JX06, the half-life of IMX in the soil is shortened to 11 d and 13 d, indicating that Streptomycetaceae had a positive effect on the remediation of soil. It is expected to provide scientific information for the rational use, environmental safety evaluation of IMX, and provide a basis for future research and development of microbial agents.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"123 1","pages":"470 - 478"},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76168798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Application electrochemical sensor based on nanosheets G-C3N4/CPE by square-wave anodic stripping voltammetric for measure amounts of toxic tartrazine color residual in different drink and foodstuffs 基于纳米片G-C3N4/CPE的电化学传感器方波阳极溶出伏安法测定不同饮料和食品中有毒酒黄色残留量
Journal of Environmental Science and Health, Part B Pub Date : 2022-04-17 DOI: 10.1080/03601234.2022.2064676
F. Marahel, L. Niknam
{"title":"Application electrochemical sensor based on nanosheets G-C3N4/CPE by square-wave anodic stripping voltammetric for measure amounts of toxic tartrazine color residual in different drink and foodstuffs","authors":"F. Marahel, L. Niknam","doi":"10.1080/03601234.2022.2064676","DOIUrl":"https://doi.org/10.1080/03601234.2022.2064676","url":null,"abstract":"Abstract The present work describes a method (SWASV) techniques for measure of tartrazine color a harmful compound present in real samples, and the extremely harmful to humans and animals even at low concentrations using G-C3N4 nanosheets sensor. Here, we report the use of an electrochemical approach for analytical determination of toxic tartrazine that takes 150 s. The calibration curve was linear in range of the (0.02–18.0 µmol L−1). The current response was linearly proportional to the tartrazine concentration with a R 2∼ 0.999. We demonstrated a sensitivity a limit of detection of (0.022 µmol L−1). Finally, sensor nanosheets G-C3N4/CPE introduced to measure toxic tartrazine in different drink and foodstuff samples was used and the chemical nanosheets G-C3N4/CPE sensor made it possible as an excellent sensor with reproducibility for determination other samples.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"41 1","pages":"489 - 496"},"PeriodicalIF":0.0,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86467718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Cumulative potential and half-life of [imazapic + imazapyr] in lowland soils of Rio Grande Do Sul grown with clearfield® rice 在南里奥格兰德州种植clearfield®水稻的低地土壤中[imazapic + imazapyr]的累积潜力和半衰期
Journal of Environmental Science and Health, Part B Pub Date : 2022-04-12 DOI: 10.1080/03601234.2022.2063613
Thais Stradioto Melo, Caroline Hernke Thiel, Laryssa Barbosa Xavier da Silva, S. Deuner, A. Andres, Gabriele Espinel Ávila, Stefânia Nunes Pires, G. Concenço
{"title":"Cumulative potential and half-life of [imazapic + imazapyr] in lowland soils of Rio Grande Do Sul grown with clearfield® rice","authors":"Thais Stradioto Melo, Caroline Hernke Thiel, Laryssa Barbosa Xavier da Silva, S. Deuner, A. Andres, Gabriele Espinel Ávila, Stefânia Nunes Pires, G. Concenço","doi":"10.1080/03601234.2022.2063613","DOIUrl":"https://doi.org/10.1080/03601234.2022.2063613","url":null,"abstract":"Abstract The objectives of this study were to estimate the residual and half-life of [imazapic + imazapyr] and to infer on the impact of these residuals over time. The first experiment comprised the application of [imazapic + imazapyr] to Clearfield® rice. On the following summer cropping season (365 days later), undeformed soil samples 0-5 cm depth were collected and seeds of six species or varieties were sown as bioindicators of residuals (experiment 2), being assessed plant height and dry mass 20 days after emergence start. The third experiment comprised the cultivation of the same species submitted to ten increasing herbicide doses (0-280 g ha−1) to establish standard response curves, also assessing plant height and dry mass 20 days after emergence start. About 2.1-5.8% of the applied imazapic remains in soil after one year, for the label doses. Imazapyr was considered to be at negligible doses as its half-life is short, and less than 0.0000001% of the applied dose is expected to be in soil 365 days later. The expected imazapic half-life in lowland areas of Southern Brazil is longer than for dryland, being estimated as between 63 and 77 days (95% confidence interval), contrasting to the 60 days half-life previously estimated for dryland soils.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"19 1","pages":"450 - 457"},"PeriodicalIF":0.0,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78831798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Graphitic-phase C3N4 nanosheets combined with MnO2 nanosheets for sensitive fluorescence quenching detection of organophosphorus pesticides 石墨相C3N4纳米片复合二氧化锰纳米片用于有机磷农药的荧光猝灭检测
Journal of Environmental Science and Health, Part B Pub Date : 2022-04-12 DOI: 10.1080/03601234.2022.2063608
Bicheng Liu, Jin Chen, Yi-An Peng, Wenyue Xiao, Zoujun Peng, P. Qiu
{"title":"Graphitic-phase C3N4 nanosheets combined with MnO2 nanosheets for sensitive fluorescence quenching detection of organophosphorus pesticides","authors":"Bicheng Liu, Jin Chen, Yi-An Peng, Wenyue Xiao, Zoujun Peng, P. Qiu","doi":"10.1080/03601234.2022.2063608","DOIUrl":"https://doi.org/10.1080/03601234.2022.2063608","url":null,"abstract":"Abstract In this study, we have developed a sensitive approach to measure organophosphorus pesticides (OPs) using graphitic-phase C3N4 nanosheets (g-C3N4) combined with a nanomaterial-based quencher, MnO2 nanosheets (MnO2 NS). Since MnO2 NS can quench the fluorescence of g-C3N4 via the inner-filter effect (IFE), enzymatic hydrolysate (thiocholine, TCh) can efficiently trigger the decomposition of MnO2 nanosheets in the presence of acetylcholinesterase (AChE) and acetylthiocholine (ATCh), resulting in the fluorescence recovery of g-C3N4. OPs, as inhibitors to AChE activity, can prevent the generation of TCh and decomposition of MnO2 nanosheets while exhibiting fluorescence quenching. Therefore, the AChE-ATCh-MnO2-g-C3N4 system can be utilized to quantitatively detect OPs based on g-C3N4 fluorescence. Under optimal conditions, the linear ranges for the determination of parathion-methyl (PM) and 2,2-dichlorovinyl dimethyl phosphate (DDVP) were found to be 0.1–2.1 ng/mL and 0.5–16 ng/mL, respectively, with limits of detection of 0.069 ng/mL and 0.20 ng/mL, respectively. The advantages of this assay are user-friendliness, ease of use, and cost effectiveness compared to other more sophisticated analytical instruments.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"5 1","pages":"441 - 449"},"PeriodicalIF":0.0,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74500571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synthesis, molecular docking and extensive structure activity relationship of substituted DHP derivatives: a new class of herbicides 一类新的除草剂取代DHP衍生物的合成、分子对接及广泛的构效关系
Journal of Environmental Science and Health, Part B Pub Date : 2022-04-10 DOI: 10.1080/03601234.2022.2062188
Akansha Agrwal, A. Verma, Neelam Chantola, S. Verma, V. Kasana
{"title":"Synthesis, molecular docking and extensive structure activity relationship of substituted DHP derivatives: a new class of herbicides","authors":"Akansha Agrwal, A. Verma, Neelam Chantola, S. Verma, V. Kasana","doi":"10.1080/03601234.2022.2062188","DOIUrl":"https://doi.org/10.1080/03601234.2022.2062188","url":null,"abstract":"Abstract In the present study, twenty-two derivatives of dihydropyridine (DHP) have been synthesized using the Boric acid catalyst in solventless conditions. The synthesis was confirmed by FTIR analysis, 1HNMR, and 13CNMR analysis. The quantitative structure-activity relationship for all the synthesized derivatives was performed using an artificial neural network with correlation coefficient (R2) 0.8611, mean standard error 0.19, and Comparative molecular field analysis (CoMFA) with correlation coefficient (R2) 0.713, mean standard error 0.27. The molecular docking activity of synthesized compounds was tested using “AUTODOCK VINA” against “Acetohydroxyacid synthase protein receptors (PDB code 1YHZ)” acquired from the “RCSB Protein Data Bank”. Docking experiments demonstrated favorable interaction among synthesized DHP derivatives and protein receptors with significant binding energy values. These synthesized derivatives have been screened for their pre-emergence herbicidal bioassay against weed species Echinochola crus galli, and the IC50 value were calculated and activity was compared with Butachlor, significant activity was exhibited by all the derivatives. All the synthesized compounds were also screened for their post emergence herbicidal activity against Echinochola crus galli, and the activity of DHPs were compared with penoxulum. All the synthesized compounds show good to moderate activity. Thus, it is concluded that substituted DHP derivatives may be developed as potential herbicides. Graphical Abstract","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"28 1","pages":"379 - 420"},"PeriodicalIF":0.0,"publicationDate":"2022-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86707925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
The aflatoxin inhibitors capsaicin and piperine from Capsicum chinense and Piper nigrum fruits modulate the antioxidant system in Aspergillus parasiticus 辣椒和黑椒果实中的黄曲霉毒素抑制剂辣椒素和胡椒碱调节了寄生曲霉体内的抗氧化系统
Journal of Environmental Science and Health, Part B Pub Date : 2022-04-08 DOI: 10.1080/03601234.2022.2060029
G. V. Buitimea-Cantúa, H. M. Leija Gutiérrez, N. E. Buitimea‐Cantúa, María Del Refugio Rocha-Pizaña, A. García-Triana, A. Hernández-Morales, E. Magaña-Barajas, J. Molina-Torres
{"title":"The aflatoxin inhibitors capsaicin and piperine from Capsicum chinense and Piper nigrum fruits modulate the antioxidant system in Aspergillus parasiticus","authors":"G. V. Buitimea-Cantúa, H. M. Leija Gutiérrez, N. E. Buitimea‐Cantúa, María Del Refugio Rocha-Pizaña, A. García-Triana, A. Hernández-Morales, E. Magaña-Barajas, J. Molina-Torres","doi":"10.1080/03601234.2022.2060029","DOIUrl":"https://doi.org/10.1080/03601234.2022.2060029","url":null,"abstract":"Abstract Several aflatoxin inhibitors can modulate the antioxidant system in fungi. In this work, the effect of the ethanolic extract of Capsicum chinense and Piper nigrum fruits, capsaicin, and piperine on the expression of the aflE, aflG, aflH, aflI, aflK, aflL, aflO, aflP, and aflQ genes involved in the aflatoxin biosynthetic pathway in Aspergillus parasiticus were studied by qRT-PCR analysis. As well as, the effect on the expression of fungal antioxidant genes (sod1, catA, and cat2) and enzymatic activity of catalase (CAT) and superoxide dismutase (SOD). Results reveal that the highest (p < 0.05) radial growth inhibition (68 and 86%) and aflatoxins production inhibition (73 and 80%) was observed with capsaicin and piperine respectively, at 300 µg/mL, instead of the ethanolic extract at the same concentration. The qRT-PCR analysis showed that compounds and extracts at 300 µg/mL induced a down-regulation of aflatoxin genes and an up-regulation on the fungal antioxidant genes. CAT activity increased by 23.15, 36.65, 51.40, and 65.50%, in the presence of C. chinense and P. nigrum extract, capsaicin, and piperine exposure, respectively. While SOD activity was not significantly impacted (p > 0.05). In conclusion, the capsaicin and piperine, two antifungal and anti-aflatoxigenic compounds produce an up-regulation of antioxidant defense genes accompanied by an enhancement of catalase enzymatic activity in A. parasiticus.","PeriodicalId":15670,"journal":{"name":"Journal of Environmental Science and Health, Part B","volume":"1 1","pages":"358 - 368"},"PeriodicalIF":0.0,"publicationDate":"2022-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88853470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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