Journal of Endocrinology and Reproduction最新文献

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Emphasizing the Role of Multi-omics Approach to Increase Survival Rate of Breast and Prostate Cancer Patients 强调多组学方法在提高乳腺癌和前列腺癌患者生存率中的作用
Journal of Endocrinology and Reproduction Pub Date : 2023-09-12 DOI: 10.18311/jer/2023/34985
Khushali Upadhyay, Foram Patel, Yashshvini Patel, A. V. Ramachandran, Darshee Baxi
{"title":"Emphasizing the Role of Multi-omics Approach to Increase Survival Rate of Breast and Prostate Cancer Patients","authors":"Khushali Upadhyay, Foram Patel, Yashshvini Patel, A. V. Ramachandran, Darshee Baxi","doi":"10.18311/jer/2023/34985","DOIUrl":"https://doi.org/10.18311/jer/2023/34985","url":null,"abstract":"The understanding of cancer biology has greatly advanced since the advent of genomics. A remarkable heterogeneity at the whole-genome (or omics) level exists amongst even histologically comparable cancers, demonstrating the enormous complexity of the cancer genome. A powerful resource that has the potential to translate high-throughput omics to better and quick overall survival is the massive accrual and public accessibility of multi-omics databases with accompanying clinical annotation, including tumor histology, patient response, and outcome. In this new era of high-throughput omics, this paper emphasizes the distinct benefits of a multidimensional approach to genomic analysis. It discusses the implications of translational omics research for the cancer population. Single-level data analysis of high-throughput technologies has constraints because it only displays a small window of cellular processes. Understanding the links across several cellular organization levels made possible by data integration across various platforms, including genomes, epigenomics, transcriptomics, proteomics, and metabolomics, is important. This review examines a few popular frameworks for integrating multi-omics data. It provides a general overview of multi-omics applications in tumor classification, prognosis, diagnostics, and the function of data integration in searching for novel biomarkers and treatment options.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135887159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Perspectives of PCOS Pathophysiology: Exploring the Interplay between PCOS and the Gut Microbiota 多囊卵巢综合征病理生理学的观点:探索多囊卵巢综合征与肠道微生物群之间的相互作用
Journal of Endocrinology and Reproduction Pub Date : 2023-09-12 DOI: 10.18311/jer/2023/34986
Komal Khade, Roshan Dadachanji, Srabani Mukherjee
{"title":"Perspectives of PCOS Pathophysiology: Exploring the Interplay between PCOS and the Gut Microbiota","authors":"Komal Khade, Roshan Dadachanji, Srabani Mukherjee","doi":"10.18311/jer/2023/34986","DOIUrl":"https://doi.org/10.18311/jer/2023/34986","url":null,"abstract":"PCOS is a gynecological and metabolic concern for numerous women of reproductive age. Its pathophysiology broadly entails hyperandrogenism, insulin resistance, and neuroendocrine dysfunction, and is heavily influenced by genetic and epigenetic factors. However, its precise aetiology remains unclear. The gut microbiome is a major endocrine organ and plays a key role in host metabolism through its metabolites which regulate diverse host physiology like metabolism, immunity, etc. Numerous studies have described associations of altered microbiota in the progress and development of major human diseases. The studies conducted on animals and humans have suggested that the gut microbiota and its metabolites are involved in the pathogenesis of PCOS and its associated attributes such as insulin resistance, obesity and inflammation. Moreover, supplementation of probiotics/prebiotics has been reported to relieve the adverse metabolic and hormonal parameters effectively. Knowledge of this link between gut dysbiosis and PCOS has also spurred research interest in exploring novel management of PCOS. In this review, we have discussed the role of gut microbiota dysbiosis and its metabolite in the progression and treatment of PCOS.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135887164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Menthol Attenuates Cholinergic Dysfunction and Neurotransmitter Imbalance in Experimental Diabetes 薄荷醇减轻实验性糖尿病胆碱能功能障碍和神经递质失衡
Journal of Endocrinology and Reproduction Pub Date : 2023-08-17 DOI: 10.18311/jer/2023/32989
S. Soumya, S. Mini
{"title":"Menthol Attenuates Cholinergic Dysfunction and Neurotransmitter Imbalance in Experimental Diabetes","authors":"S. Soumya, S. Mini","doi":"10.18311/jer/2023/32989","DOIUrl":"https://doi.org/10.18311/jer/2023/32989","url":null,"abstract":"One of the most predominant enduring consequences of Diabetes Mellitus (DM) is Diabetic Encephalopathy (DE), which has neither a reliable treatment nor an effective preventive strategy. Cognitive dysfunction is the primary problem allied with DE. The current inquiry aims to determine the potency of menthol in reducing the risk of brain complications induced by Streptozotocin (STZ) in diabetic rats. A single STZ intraperitoneal injection (40 mg/kg body weight) was employed to induce DM in Sprague-Dawley male rats and animals were held without treatment for 30 days to develop DE. The Morris water maze test, followed by the supplementation of menthol and metformin for 60 days at 50 and 100 mg/kg body weight dosages, verified the cognitive deficit in diabetic rats. After 60 days of therapy, rats were sacrificed to obtain blood and brain tissues for biochemical investigation. Oral delivery of menthol enhanced cognitive function in DE rats. Furthermore, menthol markedly reduced fasting blood sugar, glycosylated Hemoglobin (HbA1c), and elevated plasma insulin levels. In the brain, menthol increases neurotransmitter levels and choline acetyltransferase activity while decreasing AChE activity. Menthol also downregulated the expressions of monoamine oxidase A and B. Thus, the study indicates that menthol was effective in attenuating the neurodegenerative alterations in DE rats. It had a therapeutic potential and could be effectively utilized as a dietary supplement for regulating complications associated with encephalopathy.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81239362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effect of Methallibure (ICI Compound 33, 828) on Folliculogenesis and Steroidogenic Potential of Developing Ovary in the Lizard, Calotes versicolor (Daud.) 甲基甲醚(ICI化合物33,828)对花斑壁虎卵巢卵泡发生和甾体潜能的影响
Journal of Endocrinology and Reproduction Pub Date : 2023-08-17 DOI: 10.18311/jer/2023/34311
B. Y. Gouder, Rahul M. Handi, V. Vani, Gopal M. Advi Rao, Laxmi S. Inamdar (Doddamani)
{"title":"The Effect of Methallibure (ICI Compound 33, 828) on Folliculogenesis and Steroidogenic Potential of Developing Ovary in the Lizard, Calotes versicolor (Daud.)","authors":"B. Y. Gouder, Rahul M. Handi, V. Vani, Gopal M. Advi Rao, Laxmi S. Inamdar (Doddamani)","doi":"10.18311/jer/2023/34311","DOIUrl":"https://doi.org/10.18311/jer/2023/34311","url":null,"abstract":"In vertebrates, it is well established that gonadotropins are crucial for vertebrate ovarian development and its endocrine functions. The current investigation envisages determining how methallibure affects the histoanatomical structure and steroidogenic capacity of the developing ovary of 10-day-old female hatchlings of Calotes versicolor. The eggs were collected from gravid females during the breeding season and incubated at a female-producing temperature of 31.5±0.5 oC till hatching. Hatchlings were fed with live termites. Intraperitoneal injections of 6μg of methallibure in 0.05ml of 0.7% saline were injected into 10-day-old female hatchlings on alternate days for 21 days. The appropriate saline-treated hatchlings served as control. The results reveal that methallibure significantly reduces the number of oocytes (P<0.02) and primordial follicles, decreases their diameter, and increases the number of previtellogenic atretic follicles (P<0.001) with concomitant suppression of the progression of ovarian development when compared with that of baseline control. Three types of atretic follicles were noticed. The histoenzymatic activity of Δ5-3β-hydroxysteroid dehydrogenase in the ooplasm of the follicle and the stromal region showed a decreased intensity. The downregulation of steroidogenesis is probably due to the deprived secretion of gonadal steroids which disturbs the negative feedback mechanism because of the action of the drug at the hypothalamic-hypophyseal-ovarian axis. The present study unveils that methallibure acts as an antigonadotrophic drug altering the synthesis/secretion of gonadotrophin by hypophysis. It is inferred that methallibure induces an anti-gonadotropic effect in the newly hatched hatchlings probably by quenching the pituitary gonadotropins.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78458597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Maternal Di-(2-Ethylhexyl) Phthalate (DEHP) Exposure Impairs Insulin Signal in the Liver and Gastrocnemius Muscles of Female Offspring Rats 母体暴露于二-(2-乙基己基)邻苯二甲酸酯(DEHP)对雌性后代大鼠肝脏和腓肠肌胰岛素信号的影响
Journal of Endocrinology and Reproduction Pub Date : 2023-08-17 DOI: 10.18311/jer/2023/32523
Divyaa Srinivasan, Gokulapriya Rajagopal, Balasubramanian Karundevi, Kamakshi Arjunan Leela, R. S. Bhaskaran
{"title":"Maternal Di-(2-Ethylhexyl) Phthalate (DEHP) Exposure Impairs Insulin Signal in the Liver and Gastrocnemius Muscles of Female Offspring Rats","authors":"Divyaa Srinivasan, Gokulapriya Rajagopal, Balasubramanian Karundevi, Kamakshi Arjunan Leela, R. S. Bhaskaran","doi":"10.18311/jer/2023/32523","DOIUrl":"https://doi.org/10.18311/jer/2023/32523","url":null,"abstract":"Di-(2-Ethylhexyl) Phthalate (DEHP) is a potent endocrine disruptor that is commonly present in consumer products and cosmetics. Exposure to DEHP during gestational and lactational periods can adversely affect glucoregulation and lead to the onset of diabetes in progeny. The liver and gastrocnemius muscles play an important role in regulating glucose metabolism and insulin action. This study was designed to investigate the effect of maternal DEHP exposure on insulin signaling molecules in the liver and gastrocnemius muscles of adult female offspring rats. Rat dams were given DEHP (10 and 100 mg/kg b.wt./day) by oral gavage from gestation day 9 (GD 9) to the end of the lactation period Postnatal Day (PND) 21. On PND 80, female offspring rats in diestrus were euthanized and found reduced body weight, organ (liver and gastrocnemius) weight, and hyperglycemia in DEHP-exposed rats. Western blots revealed a dose-dependent reduction in the expression of Insulin Receptor - (IR), IRS, Akt, and GSK-3β proteins as well as their phosphorylated forms in the liver and gastrocnemius muscles of DEHP-exposed offspring rats. Maternal DEHP exposure reduced the levels of GLUT2 and GLUT4 level in the liver and gastrocnemius muscles, respectively. Liver and renal function markers were dose-dependently increased in the serum of offspring female rats born to DEHP exposed mother during gestation and lactation. Thus, the study revealed that maternal DEHP exposure impaired the expression of insulin signaling molecules in the two important tissues involved in glucose metabolism, the liver and gastrocnemius muscles, suggesting that phthalates exposure during development may contribute to the onset of diabetes in female offspring.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90578037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Crosstalk Between Pineal and Major Extra-Pineal Sources of Melatonin and its Role in Ovarian Growth and Maturation in Fish 松果体和主要松果体外褪黑素来源的串扰及其在鱼类卵巢生长和成熟中的作用
Journal of Endocrinology and Reproduction Pub Date : 2023-08-17 DOI: 10.18311/jer/2023/33014
Sona Sutradhar, Farha Yasmin, A. Roy, Russel Sarkar, S. Mukherjee
{"title":"A Crosstalk Between Pineal and Major Extra-Pineal Sources of Melatonin and its Role in Ovarian Growth and Maturation in Fish","authors":"Sona Sutradhar, Farha Yasmin, A. Roy, Russel Sarkar, S. Mukherjee","doi":"10.18311/jer/2023/33014","DOIUrl":"https://doi.org/10.18311/jer/2023/33014","url":null,"abstract":"Pinealocytes of the pineal gland in vertebrates mainly synthesize melatonin (5-methoxy-N-acetyl-tryptamine). Moreover, melatonin is synthesized in several extra-pineal cells, including the photoreceptor cells of the retina, the cells of the gut, and the hepatocytes of the liver in different vertebrates, including fish species. One of the remarkable features of pineal and retinal melatonin is that it is produced rhythmically in synchronization with the environmental Light-Dark (LD) cycle, with a daily nighttime peak. However, the melatonin synthesis in tissue/cells from the extra-pineal and extra-retinal origin(s) may not always undergo photoperiod-regulated daily variations but is also dependent on the environmental food entrainment factors (in the gut), acting as the most reliable synchronizer(s) in its daily rhythm features. Moreover, the regulation of the liver and ovary (important for fish reproduction) is unclear. In this review, we attempt a comparative account of the nature and regulation of endogenous melatonin synthesis between a source like the pineal gland and many other nonpineal origins, which have gained serious attention in the last ten years. We also review the functions of melatonin in regulating fish ovarian growth and maturation. The physiological melatonin levels, manipulated either endogenously (by photoperiodic modulations) or exogenously (by injections or by feeds), have tremendous effects on reproductive events in fish at the age of its first maturity, as revealed in recent findings. Characterization and identification of the importance of pineal gland melatonin in the growth of the oocytes via the hypothalamic-pituitary-gonadal axis have been explored several years back. The identification of melatonin receptors about fourteen years back on the wall of developing oocyte spurt the breakthrough, which introduced the concept of direct control of melatonin on developing oocytes. Thus, this review gains uniqueness by addressing the latest developments recorded in the field of melatonin and fish reproduction, particularly in improving oocyte maturation. Nonetheless, an attempt has been made to underline approaches that need to be developed to apply the molecule in large-scale aquaculture.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73042782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Glucocorticoid-Induced Dose-Dependent Reproductive Impairments in Male Albino Rat 糖皮质激素诱导的雄性白化大鼠剂量依赖性生殖损伤
Journal of Endocrinology and Reproduction Pub Date : 2023-08-17 DOI: 10.18311/jer/2023/34312
Sunita Patel, S. Rai
{"title":"Glucocorticoid-Induced Dose-Dependent Reproductive Impairments in Male Albino Rat","authors":"Sunita Patel, S. Rai","doi":"10.18311/jer/2023/34312","DOIUrl":"https://doi.org/10.18311/jer/2023/34312","url":null,"abstract":"The present study is aimed at elucidating the effect of different doses of Dexamethasone (DEX) on testis and epididymis of male Wistar rats. Thirty-six rats were divided into six groups, each containing six rats: one control (CON), and five groups of rats treated with five (30 μg, 40 μg, 50 μg, 60 μg, 70 μg/100 g body weight) doses of dexamethasone intraperitoneally (i.p.) for twenty-one days. The results revealed a dose-dependent decrease in testes and epididymis weight. DEX-treated rats evidenced significant increase in TBARS levels, this being the highest in 70 μg/100 g body weight (bw) which could be a consequence of the highest level of free radical generation. The activity level of antioxidative enzymes Superoxide Dismutase (SOD), Catalase (CAT), Glutathione (GSH), and protein content significantly declined in dexamethasone-treated rats in a dose-dependent manner. Histological observation revealed different degrees of germ cell degeneration/alterations in testis and epididymis The present finding suggests that exposure to dexamethasone (70 μg/100 g bw) can potentially lead to severe impairments in male reproductive tissues (testis and epididymis) structure and function, which may consequently lead to male infertility.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84289193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sexual Biology and Mating Behaviour in Decapod Crustaceans: A Case Study with Coral Reef Dwelling Caridean Shrimps 十足甲壳类动物的性生物学和交配行为:以珊瑚礁栖虾为例
Journal of Endocrinology and Reproduction Pub Date : 2023-05-19 DOI: 10.18311/jer/2023/31247
T. Subramoniam
{"title":"Sexual Biology and Mating Behaviour in Decapod Crustaceans: A Case Study with Coral Reef Dwelling Caridean Shrimps","authors":"T. Subramoniam","doi":"10.18311/jer/2023/31247","DOIUrl":"https://doi.org/10.18311/jer/2023/31247","url":null,"abstract":"Crustaceans have diversified sexual systems, incorporating various reproductive modalities such as gonochorism and a wide variety of hermaphroditism. In particular, the coral reef- dwelling caridean shrimps, living in symbiosis with other marine invertebrates, have developed unusual sexual systems that have given origin to a range of social systems, including the highly advanced eusociality. Protandric hermaphroditism, common among the caridean shrimps, has also reached a unique sexual condition, known as protandric simultaneous hermaphroditism, in the genus, Lysmata. Similarly, mating systems in the caridean shrimps are highly diverse and divergent. In addition, their occurrence in the coral reef shrimps is correlated to their life style adaptations such as their commensal association with other invertebrates. Other ecological factors like the predation risk from outside of the host animals and differences in the host characteristics may also be the driving forces in determining mating associations. Furthermore, the transition from sexual monogamy to social monogamy under conditions of their symbiotic life led to complex community living in several synalpheid shrimps. Understandably, the existence of various sexual and mating systems in these caridean shrimps has a dependence on the eco- social conditions prevalent in the coral reef biota.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84555169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Epididymis of the Lizard Eutropis carinata: A Light Microscopic and Ultrastructural Seasonal Study 棘尾蜥蜴附睾:光镜和超微结构的季节性研究
Journal of Endocrinology and Reproduction Pub Date : 2023-05-16 DOI: 10.18311/jer/2023/31257
Ivan Aranha, M. Bhagya, B. Sagar
{"title":"Epididymis of the Lizard Eutropis carinata: A Light Microscopic and Ultrastructural Seasonal Study","authors":"Ivan Aranha, M. Bhagya, B. Sagar","doi":"10.18311/jer/2023/31257","DOIUrl":"https://doi.org/10.18311/jer/2023/31257","url":null,"abstract":"The epididymis of the lizard Eutropis carinata can be divided into four regions viz., an initial segment (extra testicular) Efferent ductules (Eds) and three regions, the anterior region, a broad middle, and a narrow posterior, comparable to the caput, corpus, and cauda epididymidis of mammals. The anterior region of the epididymis is closely associated with a whitish initial segment (extra testicular), the Efferent ductules (Eds). The epididymal wall consists of an epithelial layer lining the lumen resting on a basement membrane surrounded by four to five layers of smooth circular muscles, a layer of connective tissue, and a layer of serosa. The ultrastructure study of the initial segment of epididymis the Eds during breeding season discloses four cell types in its epithelium viz., ciliated, non-ciliated, Apical-Mitochondria Rich Cells (AMRC), and basal cells. The anterior, middle, and posterior regions of the epididymis show five different cell types namely principal, basal, AMRC, narrow, and clear cells. Ciliated and non-ciliated cells are limited to the initial segment of the epididymis while, principal, narrow, and clear cells are found in the rest of the three regions of the epididymis. Basal and AMRC are found in all four regions. AMRCs are the most abundant cell type in the initial segment while principal cells are the major components of the epithelial lining of the remaining regions of the epididymis. During the non-breeding season, all the cell types are present but with regression and altered cytology of the cells without any sign of cellular activity in the different regions of the epididymis. During the breeding season, even the circulating testosterone levels are significantly higher compared to the non-breeding season. This is the first report describing different cell types in the initial segment and three different regions of the epididymis with ultrastructural seasonal variations in the Keeled Indian Mabuya, Eutropis carinata (Scincidae).","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"185 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72643384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibition of Cholesterol Biosynthesis Modulates Epithelial-Mesenchymal Transition in Primary Cicatricial Alopecia Through TGFβ and Angiotensin Receptors 抑制胆固醇生物合成通过TGFβ和血管紧张素受体调节原发性瘢痕性脱发的上皮-间质转化
Journal of Endocrinology and Reproduction Pub Date : 2023-05-12 DOI: 10.18311/jer/2023/33509
Leemon Nikhila, Suresh Surya, Shahul Hameed Najeeb, T. M. Binumon, P. P. Sreejith
{"title":"Inhibition of Cholesterol Biosynthesis Modulates Epithelial-Mesenchymal Transition in Primary Cicatricial Alopecia Through TGFβ and Angiotensin Receptors","authors":"Leemon Nikhila, Suresh Surya, Shahul Hameed Najeeb, T. M. Binumon, P. P. Sreejith","doi":"10.18311/jer/2023/33509","DOIUrl":"https://doi.org/10.18311/jer/2023/33509","url":null,"abstract":"Introduction: Primary Cicatricial Alopecia (PCA) is an autoimmune condition that affects the skin and causes hair loss in patients. In PCA the hair follicles of the patients are irreversibly damaged and replaced with fibrous tissue. This diseased condition lends relevance to our work since the fibrosis raises the potential that PCA may be affected in some way by the Epithelial Mesenchymal Transition (EMT). We used small interfering RNAs (siRNA) of TGFβ, AGTR and their regulators to identify the EMT modulation. Because these molecules mediate the induction of EMT. This study explores the idea of lowering PCA fibrosis by modifying EMT markers. Methods: We chose 7 DHC and BM15766 to investigate the function of cholesterol biosynthesis inhibition. We employed the HFORS in vitro and the mouse in vivo model system to examine EMT regulation PCA. Quantitative real-time PCR was utilised to examine the expression of genes in PCA scalp samples, compound-treated HFORS, and mouse tissues; immunohistochemistry was used to confirm the protein estimate in the scalp samples; and small interfering RNA (siRNA) transfection was used to identify the functional analysis of TGFβ and AGTR. Results: Reduced cholesterol production in PCA patients leads to permanent hair follicle damage. The in vitro and in vivo study using 7DHC and BM15766 revealed cells were positive for the EMT markers. PPARγ, AhR, and AGTR together can act as vital EMT regulators. As a result, the PPARγ agonist, AhR, and AGTR antagonist significantly downregulate the expression of CDH1, SNAIL1, and SMA. The markers of EMT are likewise deregulated by the transfection of siRNA for TGFβ and AGTR. Conclusion: We clarify how EMT is regulated in hair loss circumstances by suppressing cholesterol biosynthesis. We further confirm that EMT modulators (PPARγ, AhR, AGTR, and TGFβ) and siRNA can be employed as potentially effective strategies to slow the advancement of EMT. As a result, we propose these cholesterol and EMT modulators as potential inhibitors in PCA etiology.","PeriodicalId":15664,"journal":{"name":"Journal of Endocrinology and Reproduction","volume":"77 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91406410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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