M. Ja’afaru, M. Bashir, F. F. Tizhe, N. A. Kachalla
{"title":"Antimicrobial susceptibility profile and detection of extended spectrum β-lactamase resistance genes in pseudomonas aeruginosa isolated from post-operative wounds","authors":"M. Ja’afaru, M. Bashir, F. F. Tizhe, N. A. Kachalla","doi":"10.18488/99.v10i1.3326","DOIUrl":"https://doi.org/10.18488/99.v10i1.3326","url":null,"abstract":"The spread of Pseudomonas aeruginosa is difficult to control with disinfectants and antibiotics due to the resistance found in P. aeruginosa isolates. The study investigated the susceptibility profile and detect extended spectrum β-lactamase (ESBL) resistance genes in the Pseudomonas aeruginosa isolated from post-operative wound infection. Wound swabs were cultured on MacConkey and sub-cultured on Cetrimide agar. The isolates were identified base on their macroscopic and microscopic characteristics as well as 16SrRNA. Disc diffusion technique was used to check the susceptibility profile of the isolates and the extended spectrum beta-lactam (ESBL) producers were determined by growing the isolates on Mueller Hinton agar plate in the presence of amoxyclav. 40 positive isolates were recovered in the study with 21 isolates been multi-drug resistance (MDR). Before plasmid curing, Streptomycin, ceftazidime, Augmentin, and Gentamicin has the highest resistance rate of 80% (n=32), 60% (n=24), 60% (n=24) and 60% (n=24) respectively. However, after plasmid curing, the result showed reduction in resistance rates. Streptomycin and ceftazidime showed high reduction in resistance from 80% to 25% and from 60 to 12.7% respectively indicating that resistance was plasmid-mediated. Pseudomonas aeruginosa F23 and Pseudomonas aeruginosa S15 isolates shown to possess both the plcH and bla PER genes with bands amplicons size of 307bp and 520bp respectively. Antibiotic susceptibility pattern after plasmid curing showed that 45% of the isolates produces beta-lactamase enzymes i.e., 18 out of 40 isolates were inactive to all beta-lactam group of antibiotics.","PeriodicalId":15647,"journal":{"name":"Journal of Diseases Monitor & Control","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79594527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. A. Rahim, M. A. F. Mahmud, Mohd Farihan Md Yatim
{"title":"Key Breeding Containers of Dengue Vectors in Outbreak Localities in Federal Territory of Kuala Lumpur","authors":"F. A. Rahim, M. A. F. Mahmud, Mohd Farihan Md Yatim","doi":"10.18488/JOURNAL.99.2021.81.1.8","DOIUrl":"https://doi.org/10.18488/JOURNAL.99.2021.81.1.8","url":null,"abstract":"Dengue is a mosquito-borne disease that rapidly spreads throughout tropical and subtropical regions. An estimated 2.5 billion people live in dengue-endemic countries and millions of cases occurring each year. Identifying key breeding containers are important to prevent dengue epidemics. This study aims to identify key breeding containers and to evaluate the risks of dengue transmission in the dengue outbreak localities in Kepong District, Kuala Lumpur. Entomological surveillance was done between January 2016 to December 2017 in 38 different outbreak localities. Both indoors and outdoors water-holding containers were inspected for the presence of mosquito larvae. All collected larvae have identified the species, types of breeding containers were recorded, and three larval indices, House Index (HI), Container Index (CI), and Breteau Index (BI), were calculated. Of the 2,067 containers inspected, 227 (10.98%) containers were positive for Aedes larvae. The four main breeding container types in this study were plastic containers (31.72%), water drums (22.47%), drains (18.06%), and flowerpots (14.98%). The HI, CI, and BI varied from 0 to 52.94, from 1.72 to 32.76, and from 2.33 to 75.00, respectively. Key breeding containers of Aedes larvae found in this study provide guidance for health service providers to educate residents to eliminate the common indoors and outdoors Aedes mosquito breeding containers in the study area.","PeriodicalId":15647,"journal":{"name":"Journal of Diseases Monitor & Control","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89408461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Whelan Shane, Keaney Daniel, Lucey Brigid, F. Karen
{"title":"A Systematic Analysis of Laboratory-Guided Diagnosis and Management of Covid-19: Challenges and Recommendations","authors":"Whelan Shane, Keaney Daniel, Lucey Brigid, F. Karen","doi":"10.18488/journal.99.2020.71.14.32","DOIUrl":"https://doi.org/10.18488/journal.99.2020.71.14.32","url":null,"abstract":"We conducted a comprehensive analysis of patient demographics and laboratory tests encompassing Real Time PCR (RT-qPCR) and serology for SARS-CoV-2 in addition to blood components and clinical blood markers for COVID-19 disease. All relevant literature was included up to 15 July 2020 and multiple studies were analysed in tandem to correlate findings. For RT-qPCR, nasopharyngeal swabs are the most suitable samples based on detection rates by sample, but may require repeat testing. One-week post-symptom onset, serological testing is a more stable marker. Antibody titers have been linked to disease severity. Several clinical blood components and markers have been reported to be prognostically useful; however, care is needed when making interpretation owing to the association between raised levels of these being found for the co-morbidities that predispose to worse prognosis in COVID-19. Challenges in the current study when finding the information presented in this paper suggest the need for a quality assured database that outlines the complete set of results, anonymised patient data for each entry and a set of internationally-agreed guidelines for complete laboratory testing, documentation and open-access reporting. We suggest that such information would be useful to help with patient diagnosis and management, worldwide. 8-14, addition a positive RT-qPCR test for SARS-CoV-2 from an upper respiratory tract Antibody levels using specificity assays 99.1% antibodies), 98.6% and The seroconversion rate for total antibody 93.1% (161/173), 82.7% (143/173) for IgM, and 64.7% for IgG. remained seronegative for total antibody, to unavailability of serum for testing the later stage (<13 days PSO) of their time for seroconversion day (total antibodies), day (IgM) and (IgG)","PeriodicalId":15647,"journal":{"name":"Journal of Diseases Monitor & Control","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85090497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Vital Statistics for Leading Countries in COVID-19 Cases","authors":"Z. Singh","doi":"10.18488/journal.99.2020.71.6.13","DOIUrl":"https://doi.org/10.18488/journal.99.2020.71.6.13","url":null,"abstract":"Coronavirus disease (COVID-19) is an infectious disease caused by a newly discovered coronavirus and the overall number of the COVID-19 cases is increasing worldwide. As on 24th March, 2020, China, Italy and United States were found to be the countries with maximum number of reported cases. China seemed to manage this outbreak with only 3,281 deaths for 81,218 cases with a death rate of 0.040 and a recovery record of 73,650 patients. The present review emphasizes the comparative patterns with which this pandemic spread in the above mentioned three countries. Paper also discusses total cases, daily recorded cases and total deaths of patients with daily death records. Conclusively, COVID-19 is affecting the population globally at a very rapid rate and should be dealt with maximum efforts by all the countries. Contribution/Originality: This study documents the comparative patterns with which this pandemic spread among the leading countries including China, Italy and United States of America. The present paper also discusses total cases, daily recorded cases and total deaths of patients with daily death records.","PeriodicalId":15647,"journal":{"name":"Journal of Diseases Monitor & Control","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83507726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ato Kwamena Tetteh, Charles Baffe, Prince Bram, S. Arthur, Godsway Aglago, E. Agyarko
{"title":"Chronic Hepatitis B Virus Infection in Four (4) Asymptomatic Prospective Employees: A Short Correspondence","authors":"Ato Kwamena Tetteh, Charles Baffe, Prince Bram, S. Arthur, Godsway Aglago, E. Agyarko","doi":"10.18488/journal.99.2020.71.1.5","DOIUrl":"https://doi.org/10.18488/journal.99.2020.71.1.5","url":null,"abstract":"Aim: This study aimed at compiling all hepatitis B results for prospective employees screened at the Cape Coast Metropolitan Hospital. This information is relevant in identifying those who are already infected, as well as susceptible individuals, to promote the establishment of prevention and control measures within work environments. Method: This study extracted data from laboratory records into Microsoft Excel and used for the analysis. Results: In this report, 13.3% (4/30) of prospective employees were found to be sero-positive for hepatitis B surface antigen (HBsAg). The remaining were sero-negative (susceptible), and required vaccination. All of the four (4) found to be sero-positive were chronically infected [both hepatitis B envelope antibody (HBeAb) and core antibody (HBcAb) - positive], that is, capable of transmitting to susceptible individuals if the proper infection prevention practices are not well implemented in their homes and work places. Only males were found to be positive, while all females were negative. Conclusion: Continuous education, screening, management of infected individuals and vaccination of the susceptible, will go a long way to curbing the spread of hepatitis B infection. Contribution/Originality: This study contributes to existing information, and draws attention to occupational support in the management of hepatitis B infection.","PeriodicalId":15647,"journal":{"name":"Journal of Diseases Monitor & Control","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75747098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Occurrence of Diabetes among Rural Women","authors":"J. Randhawa, Z. Singh, Sonia","doi":"10.18488/journal.99.2020.72.33.38","DOIUrl":"https://doi.org/10.18488/journal.99.2020.72.33.38","url":null,"abstract":"Diabetes has already been described as an epidemic, and health care system around the world is bearing its pressure. Globally, it has to be dealt with huge care. In the present study, 1001 rural women (Age 21-90 years; mean age: 54 ± 3.2 years) from the northern region of Punjab were analyzed for the serum glucose levels, to estimations its occurrence/prevalence. The subjects were divided into different age groups. The study revealed a high percentage (46.2%) of subjects with serum glucose levels higher than the normal range of 70-110 mg/dl of blood. Age groups of 21-30 and 81-90 years showed a less prevalence of diabetes, whereas age groups between 31-40 and 41-50 years is most prone showing higher percentages (average 12.9 and 18.5%, respectively). The study is an indicative of a major crisis in the coming times. The data suggests that awareness drives and remedial measures should be enforced with immediate effect. Contribution/Originality: This study contributes to the existing literature related to prevalence of diabetes in India. This study is one of very few studies which have investigated the prevalence and awareness of diabetes among women of rural areas of Punjab. The paper's primary contribution is finding that rural women in age groups between 31-50 are most prone to diabetes.","PeriodicalId":15647,"journal":{"name":"Journal of Diseases Monitor & Control","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79068374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Self-Esteem, Optimism and Burden of Care among Relatives of Cardiovascular Disease Patients in Selected Tertiary Hospitals in Southwest, Nigeria","authors":"S. Babatunde, Taofik Olatunji Bankole, R. Yinusa","doi":"10.18488/journal.99.2019.62.23.33","DOIUrl":"https://doi.org/10.18488/journal.99.2019.62.23.33","url":null,"abstract":"Article History Received: 12 March 2019 Revised: 15 April 2019 Accepted: 21 May 2019 Published: 18 July 2019","PeriodicalId":15647,"journal":{"name":"Journal of Diseases Monitor & Control","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87234238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determinant Factors and Spatial Distribution of Visceral Leishmaniasis in North Gondar in Selected Hospitals","authors":"Berhanehiwot Melesse, Salie Ayalew, Mandefro Abere","doi":"10.18488/journal.99.2019.62.81.89","DOIUrl":"https://doi.org/10.18488/journal.99.2019.62.81.89","url":null,"abstract":"Article History Received: 18 July 2019 Revised: 21 August 2019 Accepted: 24 September 2019 Published: 12 November 2019","PeriodicalId":15647,"journal":{"name":"Journal of Diseases Monitor & Control","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85041456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Etim, Ezekiel Friyanda Chagwa, E. Obeagu, Akpan Akaninyene Udokang
{"title":"Staining Effect of Methanolic Extract of Hibiscus Sabdariffa Calyx on Thin Peripheral Blood Smear","authors":"E. Etim, Ezekiel Friyanda Chagwa, E. Obeagu, Akpan Akaninyene Udokang","doi":"10.18488/journal.99.2019.61.1.6","DOIUrl":"https://doi.org/10.18488/journal.99.2019.61.1.6","url":null,"abstract":"Article History Received: 9 October 2018 Revised: 14 November 2018 Accepted: 19 December 2018 Published: 4 February 2019","PeriodicalId":15647,"journal":{"name":"Journal of Diseases Monitor & Control","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87480444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}