Journal of Economic Entomology最新文献_第8页

Acaricide exposure impairs predatory behavior of the phytoseiid mite Neoseiulus idaeus (Acari: Phytoseiidae). 杀螨剂暴露会影响拟新绥螨的捕食行为。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-08-10 DOI: 10.1093/jee/toad127

Eduardo P de Sousa Neto, Jairo de Almeida Mendes, Rosenya Michely Cintra Filgueiras, Debora B de Lima, Raul Narciso C Guedes, José W S Melo

{"title":"Acaricide exposure impairs predatory behavior of the phytoseiid mite Neoseiulus idaeus (Acari: Phytoseiidae).","authors":"Eduardo P de Sousa Neto, Jairo de Almeida Mendes, Rosenya Michely Cintra Filgueiras, Debora B de Lima, Raul Narciso C Guedes, José W S Melo","doi":"10.1093/jee/toad127","DOIUrl":"https://doi.org/10.1093/jee/toad127","url":null,"abstract":"Predation is an important interaction that can change the structure of arthropod communities across both temporal and spatial scales. In agricultural systems predation can reduce the population levels of several arthropod pest species of a community. This predator-prey interaction involves the predator searching and handling behaviors. Several factors can affect this interaction, such as pesticide exposure, which is a frequent feature in agroecosystems. Thus, the hypothesis of our study is that the predatory behavior of the phytoseiid mite Neoseiulus idaeus Denmark & Muma, an important natural enemy of spider mites, is affected by acaricide exposure. To test that hypothesis, the predatory mite was exposed to the acaricides abamectin, fenpyroximate, and azadirachtin in 4 exposure scenarios. The predatory behavior of N. idaeus was negatively affected by acaricide exposure when the leaf surface containing both prey and predator was sprayed leading to a reduction in the frequency of transitions between predator walking and meeting preys. Prey handling and consumption were also compromised by acaricide exposure through contaminated leaf surface and prey, and contaminated leaf surface, prey, and predator. Abamectin compromised predation regardless of the exposure scenario. Acaricide-exposure reduced the number of prey found, number of attacks, and number prey killed by N. idaeus. Moreover, partial prey consumption was observed with acaricide-exposed mites. Thus, caution is necessary while attempting to integrate acaricide applications and mass release of N. idaeus for spider mite management.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10006734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating the insecticidal mechanisms of zein nanoparticles on Anticarsia gemmatalis (Lepidoptera: Erebidae). 玉米蛋白纳米粒杀虫机理的研究。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-08-10 DOI: 10.1093/jee/toad091

Colin A R Bonser, Carlos E Astete, Cristina M Sabliov, Jeffrey A Davis

{"title":"Elucidating the insecticidal mechanisms of zein nanoparticles on Anticarsia gemmatalis (Lepidoptera: Erebidae).","authors":"Colin A R Bonser, Carlos E Astete, Cristina M Sabliov, Jeffrey A Davis","doi":"10.1093/jee/toad091","DOIUrl":"https://doi.org/10.1093/jee/toad091","url":null,"abstract":"Previous research suggested that positively charged zein nanoparticles [(+)ZNP] were toxic to neonates of Anticarsia gemmatalis Hübner and deleterious to noctuid pests. However, specific modes of action for ZNP have not been elucidated. Diet overlay bioassays attempted to rule out the hypothesis that A. gemmatalis mortality was caused by surface charges from component surfactants. Overlay bioassays indicated that negatively charged zein nanoparticles [(-)ZNP] and its anionic surfactant, sodium dodecyl sulfate (SDS), exhibited no toxic effects when compared to the untreated check. Nonionic zein nanoparticles [(N)ZNP] appeared to increase mortality compared to the untreated check, though larval weights were unaffected. Overlay results for (+)ZNP and its cationic surfactant, didodecyldimethylammonium bromide (DDAB), were found to be consistent with former research indicating high mortalities, and thus, dosage response curves were conducted. Concentration response tests found the LC50 for DDAB on A. gemmatalis neonates was 208.82 a.i./ml. To rule out possible antifeedant capabilities, dual choice assays were conducted. Results indicated that neither DDAB nor (+)ZNP were antifeedants, while SDS reduced feeding when compared to other treatment solutions. Oxidative stress was tested as a possible mode of action, with antioxidant levels used as a proxy for reactive oxygen species (ROS) in A. gemmatalis neonates, which were fed diet treated with different concentrations of (+)ZNP and DDAB. Results indicated that both (+)ZNP and DDAB decreased antioxidant levels compared to the untreated check, suggesting that both (+)ZNP and DDAB may inhibit antioxidant levels. This paper adds to the literature on potential modes of action by biopolymeric nanoparticles.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10376557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproductive biology of overwintering leaffooted bug Leptoglossus zonatus (Hemiptera: Coreidae) in California. 加利福尼亚越冬叶脚虫 Leptoglossus zonatus（半翅目：科）的繁殖生物学。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-08-10 DOI: 10.1093/jee/toad097

Judith M Stahl, Kent M Daane

{"title":"Reproductive biology of overwintering leaffooted bug Leptoglossus zonatus (Hemiptera: Coreidae) in California.","authors":"Judith M Stahl, Kent M Daane","doi":"10.1093/jee/toad097","DOIUrl":"10.1093/jee/toad097","url":null,"abstract":"Leptoglossus zonatus (Dallas) (Hemiptera: Coreidae) is a polyphagous insect pest attacking a wide variety of crops. In California's Central Valley, it is now the dominant leaffooted bug on almonds, pistachios, and pomegranates. Leptoglossus zonatus pest status depends largely on overwintering adult survival and reproductive potential, which determines its population size in spring and early summer when nut crops are particularly susceptible to bug damage. Here, we investigated the overwintering reproductive biology of L. zonatus in laboratory and field experiments to gain information about its ovary development, time of mating, and the impact of low temperatures on egg hatch. With dissections of laboratory-reared L. zonatus, we established a baseline for ovarian development and determined that the size of the spermathecal reservoir is larger in mated than in unmated females. Dissections and behavioral experiments of field-collected material provided evidence of mating events before dispersal from overwintering sites. Laboratory trials showed that temperature significantly impacted L. zonatus egg hatch. Leptoglossus zonatus reproductive biology presented provides valuable information on its population dynamics and dispersal from overwintering sites, and will contribute to the development of monitoring and management tools.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10376558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of different traps and attractants in 3 food processing facilities in Greece on the capture of stored product insects. 希腊 3 家食品加工厂使用不同诱捕器和引诱剂捕捉储藏产品昆虫的比较。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-08-10 DOI: 10.1093/jee/toad107

William R Morrison, Paraskevi Agrafioti, Michael J Domingue, Deanna S Scheff, Evagelia Lampiri, Marina Gourgouta, Georgia V Baliota, Maria Sakka, Scott W Myers, Christos G Athanassiou

{"title":"Comparison of different traps and attractants in 3 food processing facilities in Greece on the capture of stored product insects.","authors":"William R Morrison, Paraskevi Agrafioti, Michael J Domingue, Deanna S Scheff, Evagelia Lampiri, Marina Gourgouta, Georgia V Baliota, Maria Sakka, Scott W Myers, Christos G Athanassiou","doi":"10.1093/jee/toad107","DOIUrl":"10.1093/jee/toad107","url":null,"abstract":"Certain lures are marketed toward particular pests or classes of pests, while others might be multi-species lures. Investigative aims for this study included both which trap was most sensitive and whether different combinations of traps and attractants were delivering novel information about the stored product insect community. Comparisons were made for all combinations of 3 commercial traps and 4 different attractants plus an untreated control on the capture of stored-product insects for 2 consecutive years in 3 food processing facilities in Central Greece. The traps used in the experiments were Dome Trap (Trécé Inc., USA), Wall Trap (Trécé) and Box Trap (Insects Limited, Ltd., USA). The attractants that were evaluated were 0.13 g of (i) PantryPatrol gel (Insects Limited), (ii) Storgard kairomone food attractant oil (Trécé), (iii) wheat germ (Honeyville, USA), and (iv) Dermestid tablet attractant (Insects Limited). The traps were inspected approximately every 15 days and rotated. A total of 34,000+ individuals were captured belonging to 26 families and at least 48 species. The results indicated that Indian meal moth, Plodia interpunctella (Hübner), red flour beetle, Tribolium castaneum (Herbst), and cigarette beetle, Lasioderma serricorne (F.) were the most abundant. Although there were noticeable differences among the different traps and attractants for specific species, all combinations provided similar information on population dynamics. Generally, Dome traps baited with either the oil or the gel, were found to be the most sensitive. The results of the present study demonstrate the importance of long-term trapping protocols, as a keystone in IPM-based control strategies in food processing facilities.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9994731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chlorpyrifos and chlorfenapyr resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) relies on UDP-glucuronosyltransferases. 果夜蛾(鳞翅目:夜蛾科)对毒死蜱和杀虫腈的抗性依赖于udp -葡萄糖醛基转移酶。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-08-10 DOI: 10.1093/jee/toad088

Xiang-Ning Su, Chuan-Ying Li, Yu-Ping Zhang

{"title":"Chlorpyrifos and chlorfenapyr resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) relies on UDP-glucuronosyltransferases.","authors":"Xiang-Ning Su, Chuan-Ying Li, Yu-Ping Zhang","doi":"10.1093/jee/toad088","DOIUrl":"https://doi.org/10.1093/jee/toad088","url":null,"abstract":"Fall armyworm, Spodoptera frugiperda (J. E. Smith), has become an important agricultural pest worldwide. S. frugiperda is mainly controlled by the chemical insecticides, whereas the frequent application of insecticides would result in the resistance development. Insect uridine diphosphate-glucuronosyltransferases (UGTs), as phase II metabolism enzymes, play vital roles in the breakdown of endobiotic and xenobiotics. In this study, 42 UGT genes were identified by RNA-seq, including 29 UGT genes were elevated compared to the susceptible population, and the transcript levels of 3 UGTs (UGT40F20, UGT40R18, and UGT40D17) were increased by more than 2.0-fold in the field populations. Expression pattern analysis revealed that S. frugiperda UGT40F20, UGT40R18, and UGT40D17 were increased by 6.34-, 4.26-, and 8.28-fold, compared the susceptible populations, respectively. The expression of UGT40D17, UGT40F20, and UGT40R18 was affected after exposure to phenobarbital, chlorpyrifos, chlorfenapyr, sulfinpyrazone, and 5-nitrouracil. The induced expression of UGT genes may have improved UGT enzymatic activity, while the inhibition of UGTs genes expression may decreased UGT enzymatic activity. Sulfinpyrazone, and 5-nitrouracil, significantly increased the toxicity of chlorpyrifos and chlorfenapyr, as well as phenobarbital significantly reduced the toxicity of chlorpyrifos and chlorfenapyr against the susceptible populations and field populations of S. frugiperda. The suppression of UGTs (UGT40D17, UGT40F20, and UGT40R18) significantly increased the insensitivity of the field populations to chlorpyrifos and chlorfenapyr. These findings strongly supported our viewpoint that UGTs may play a critical role in insecticide detoxification. This study provides a scientific basis for the management of S. frugiperda.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10003381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological traits of the zoophytophagous predatory mirid Nesidiocoris poppiusi (Heteroptera: Miridae), a candidate biocontrol agent in China. 植食性捕食性稻螟(稻螟科)生物学特性研究。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-08-10 DOI: 10.1093/jee/toad087

Lili Wang, Yibo Zhang, Ningxing Huang, Ning Di, Lixia Tian, Zhengyang Zhu, Junxiu Liu, Su Wang

{"title":"Biological traits of the zoophytophagous predatory mirid Nesidiocoris poppiusi (Heteroptera: Miridae), a candidate biocontrol agent in China.","authors":"Lili Wang, Yibo Zhang, Ningxing Huang, Ning Di, Lixia Tian, Zhengyang Zhu, Junxiu Liu, Su Wang","doi":"10.1093/jee/toad087","DOIUrl":"https://doi.org/10.1093/jee/toad087","url":null,"abstract":"Mirid predators are increasingly used in biological control of multiple greenhouse crops pests. However, due to great morphological similarity and tiny body size, some mirid predators have been largely confused with their allied species. Nesidiocoris tenuis Reuter as a commercial mirid predator was confused largely with Nesidiocoris poppiusi Carvalho in China. To evaluate the biocontrol potential of N. poppiusi, its biological traits and the functional response to Bemisia tabaci Gennadius were studied compared with N. tenuis under laboratory conditions. The results showed that no significant differences of the developmental times from the first instar to adult stages between the 2 mirids fed on Corcyra cephalonica Stainton eggs were observed, while N. poppiusi had better population growth parameters than N. tenuis. Under the condition with prey, both female and male of N. poppiusi lived significantly longer than those of N. tenuis. It could lay 74.0 eggs, which was significantly higher than that of N. tenuis (30.2 eggs). Under the condition without prey, both N. poppiusi and N. tenuis couldn't complete development to adulthood on tomato, tobacco, muskmelon, and cabbage leaves, however, tobacco and tomato were more suitable than the other 2 plants. A type II functional response was observed for both males and females of the 2 predators. Nesidiocoris poppiusi females consumed significantly more B. tabaci pupae than N. tenuis when prey densities were large than 30. Our results indicated that N. poppiusi could be a promising candidate for biological control of B. tabaci.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10004162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An accurate, efficient, and economical identification technology for black twig borer based on species-specific cytochrome C oxidase subunit I PCR assay. 一种基于种特异性细胞色素C氧化酶亚基I的准确、高效、经济的黑枝螟鉴定技术。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-08-10 DOI: 10.1093/jee/toad119

Xueting Sun, Guangliang Lu, Ronghua Sun, You Li, Shiwei Sun, Lei Gao

{"title":"An accurate, efficient, and economical identification technology for black twig borer based on species-specific cytochrome C oxidase subunit I PCR assay.","authors":"Xueting Sun, Guangliang Lu, Ronghua Sun, You Li, Shiwei Sun, Lei Gao","doi":"10.1093/jee/toad119","DOIUrl":"https://doi.org/10.1093/jee/toad119","url":null,"abstract":"Xylosandrus compactus (Eichhoff) (Coleoptera: Curculionidae, Scolytinae) is a worldwide invasive species that causes huge economic loss and environmental damage in many countries. Traditional morphological characteristics make it hard to identify scolytines due to their tiny size. Besides, the intercepted insect samples are incomplete, and the limitation of insect (larvae and pupae) morphology makes morphological identification more difficult. The majority of the damage is caused by adults and fungi that serve as nutrition for their larvae. They destroy plant trunks, branches, and twigs, affecting plant transport tissues in both weak and healthy plants. An accurate, efficient, and economical molecular identification technique for X. compactus not restricted by professional taxonomic knowledge is necessary. In the present study, a molecular identification tool based on the mitochondrial DNA gene, cytochrome C oxidase subunit I (COI) was developed. A species-specific COI (SS-COI) PCR assay was designed to identify X. compactus regardless of the developmental stage. Twelve scolytines commonly found in eastern China, namely Xylosandrus compactus, X. crassiusculus, X. discolor, X. germanus, X. borealis, X. amputates, X. eupatorii, X. mancus, Xyleborinus saxesenii, Euwallacea interjectus, E. fornicatus, and Acanthotomicus suncei, were included in the study. Additionally, specimens of X. compactus from 17 different areas in China, as well as a specimen collected from the United Stated, were also analyzed. Results demonstrated the accuracy and high efficiency of the assay, regardless of the developmental stage or the type of specimen. These features provide a good application prospect for fundamental departments and can be used to prevent the harmful consequences of the spread of X. compactus.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9994740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing a rapid LAMP assay for discrimination of Drosophila suzukii (Diptera: Drosophilidae) from common drosophilids captured in monitoring traps from the Midwest, United States. 优化美国中西部监测陷阱捕获的苏氏果蝇(双翅目:果蝇科)和普通果蝇的LAMP快速鉴别方法。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-08-10 DOI: 10.1093/jee/toad105

Zihan Hong, Andrew P Michel, Elizabeth Y Long

{"title":"Optimizing a rapid LAMP assay for discrimination of Drosophila suzukii (Diptera: Drosophilidae) from common drosophilids captured in monitoring traps from the Midwest, United States.","authors":"Zihan Hong, Andrew P Michel, Elizabeth Y Long","doi":"10.1093/jee/toad105","DOIUrl":"https://doi.org/10.1093/jee/toad105","url":null,"abstract":"Spotted-wing Drosophila, Drosophila suzukii, is an economically important pest of small fruits worldwide. Currently, the timing of management strategies relies on detection of adult flies captured in baited monitoring traps; however, identifying D. suzukii in trap catch based on morphology can be challenging for growers. DNA-based diagnostic methods such as loop-mediated isothermal amplification (LAMP) have the potential to improve D. suzukii detection. This study evaluated a LAMP assay as a diagnostic tool to discriminate between D. suzukii and closely related drosophilid species found commonly in monitoring traps in the Midwestern United States. Targeting the mitochondrial cytochrome oxidase I (COI) gene, we found the LAMP assay accurately detected D. suzukii with as little as 0.1 ng/μl of DNA at 63 °C for 50 min. Under these optimal incubation conditions, D. suzukii could be discriminated from D. affinis and D. simulans consistently, when specimens collected from liquid monitoring traps were tested independently. Compared to other DNA-based diagnostic tools for D. suzukii, LAMP offers unique benefits: DNA extraction is not required, testing occurs at one temperature in less than 1 h, and positive results are visible as a colorimetric change from pink to yellow. The LAMP assay for D. suzukii can reduce reliance on morphological identification, enhance the adoption of monitoring tools, and improve accuracy of detection. Further optimization can be conducted to evaluate the accuracy and sensitivity of results when a mixture of DNA from both D. suzukii and congener flies are tested in a single LAMP reaction.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10377009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lack of fitness costs in dsRNA-resistant Leptinotarsa decemlineata ([Coleoptera]: [Chrysomelidae]). 抗dsrna的瘦蛾(Leptinotarsa decemlineata，[鞘翅目]:[chryssomelidae])缺乏适应成本。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-08-10 DOI: 10.1093/jee/toad095

M M D Pinto, R Ferreira Dos Santos, S A De Bortoli, W Moar, J L Jurat-Fuentes

{"title":"Lack of fitness costs in dsRNA-resistant Leptinotarsa decemlineata ([Coleoptera]: [Chrysomelidae]).","authors":"M M D Pinto, R Ferreira Dos Santos, S A De Bortoli, W Moar, J L Jurat-Fuentes","doi":"10.1093/jee/toad095","DOIUrl":"https://doi.org/10.1093/jee/toad095","url":null,"abstract":"The Colorado potato beetle, Leptinotarsa decemlineata (Say) ([Coleoptera]: [Chrysomelidae]), is the most important defoliator of solanaceous plants worldwide. This insect displays a notorious ability in adapting to biological and synthetic insecticides, although in some cases this adaptation carries relevant fitness costs. Insecticidal gene silencing by RNA interference is a novel mode of action pesticide against L. decemlineata that is activated by ingestion of a double stranded RNA (dsRNA) targeting a vital L. decemlineata gene. We previously reported laboratory selection of a > 11,000-fold resistant strain of L. decemlineata to a dsRNA delivered topically to potato leaves. In this work, we tested the existence of fitness costs in this dsRNA-resistant colony by comparing biological parameters to the parental strain and an additional susceptible reference strain. Biological parameters included length of egg incubation period, number of eggs per clutch, egg viability, larval viability, length of larval and pupal periods, adult emergence, number of eggs laid per day, sex ratio, and adult longevity. Comparisons between the 3 beetle strains detected no fitness costs associated with resistance to dsRNA. This information is important to guide effective insect resistance management plans for dsRNA insecticides against L. decemlineata applied topically to potato leaves.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10356890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Comparative analysis of 3 pollen sterilization methods for feeding bumble bees. 修正:3种喂养大黄蜂的花粉灭菌方法的比较分析。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-08-10 DOI: 10.1093/jee/toad124

{"title":"Correction to: Comparative analysis of 3 pollen sterilization methods for feeding bumble bees.","authors":"","doi":"10.1093/jee/toad124","DOIUrl":"https://doi.org/10.1093/jee/toad124","url":null,"abstract":"Pollen is an essential component of bee diets, and rearing bumble bees (Bombus spp.) for commercial use necessitates feeding pollen in mass quantities. This pollen is collected from honey bee (Apis mellifera L.) colonies because neither an artificial diet nor an economical, large-scale pollen collection process from flowers is available. The provenance of honey bee-collected pollen is often unknown, and in some cases has crossed international borders. Both deformed wing virus (DWV) and the fungal pathogen Ascosphaera apis (Claussen) Olive & Spiltoir (cause of chalkbrood disease); occur in honey bee-collected pollen, and infections have been observed in bumble bees. We used these pathogens as general surrogates for viruses and spore-forming fungal diseases to test the efficacy of 3 sterilization methods, and assessed whether treatment altered pollen quality for the bumble bee. Using honey bee-collected pollen spiked with known doses of DWV and A. apis, we compared gamma irradiation (GI), ozone fumigation (OZ), and ethylene oxide fumigation (EO) against an untreated positive control and a negative control. Following sterilization treatments, we tested A. apis spore viability, detected viral presence with PCR, and tested palatability to the bumble bee Bombus impatiens Cresson. We also measured bacterial growth from pollens treated with EO and GI. GI and EO outperformed OZ treatment in pathogen suppression. EO had the highest sterilizing properties under commercial conditions and retained palatability and supported bee development better than other treatments. These results suggest that EO sterilization reduces pathogen risks while retaining pollen quality as a food source for rearing bumble bees. Graphical Abstract","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9963840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1