Journal of Economic Entomology最新文献_第5页

Burkholderia spp.-based biopesticide controls wireworms (Coleoptera: Elateridae) in potatoes. 基于伯克霍尔德菌属的生物杀虫剂控制马铃薯中的线虫（鞘翅目：鞘翅科）。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-10-10 DOI: 10.1093/jee/toad146

Mika K Pagani, Timothy B Johnson, Hélène B Doughty, Kelly C McIntyre, Thomas P Kuhar

{"title":"Burkholderia spp.-based biopesticide controls wireworms (Coleoptera: Elateridae) in potatoes.","authors":"Mika K Pagani, Timothy B Johnson, Hélène B Doughty, Kelly C McIntyre, Thomas P Kuhar","doi":"10.1093/jee/toad146","DOIUrl":"10.1093/jee/toad146","url":null,"abstract":"Wireworms (Coleoptera: Elateridae) are economically significant pests of potatoes (Solanum tuberosum), damaging the marketable portion of the crop by feeding and tunneling into tubers. While conventional potato growers use the few registered synthetic insecticides to control wireworms, certified organic growers are left with less options due to the limited effectiveness of the available insecticides. Biologically derived pesticides provide an additional alternative for both systems. Certain gram-negative proteobacteria, such as Burkholderia spp., possess insecticidal compounds. However, very little is known about their efficacy on wireworms. From 2018 to 2021, we conducted experiments in Virginia to assess the efficacy of a Burkholderia spp.-based commercial pesticide, Majestene, as a wireworm control in potatoes. In a lab experiment, soil drench application of this insecticide at a rate of 66 g a.i. per 1 liter resulted in 30% wireworm mortality and significantly reduced wireworm feeding damage on potato tubers. In the field, in-furrow applications of Burkholderia spp. at a rate of 17.66 kg a.i. per ha significantly reduced wireworm damage to tubers in 2 of 7 field experiments conducted. By comparison, the commercial standard insecticide, bifenthrin, significantly reduced tuber damage in 3 of the 7 field experiments. Our study demonstrates the prospect for proteobacteria-derived insecticides for control of wireworms and potentially other soil-dwelling insects. In conclusion, findings present growers with another option to combat wireworm pressure, especially in organic systems.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":" ","pages":"1934-1938"},"PeriodicalIF":2.2,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9851683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of Scirtothrips dorsalis (Thysanoptera: Thripidae) cryptic species complex in the United States and reproductive host assessment of its dominant member. 美国背刺蓟马（Thysanoptera：Thripidae）隐蔽物种复合体的分布及其优势成员的繁殖宿主评估。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-10-10 DOI: 10.1093/jee/toad138

Vivek Kumar, Yingfang Xiao, Matthew A Borden, Muhammad Z Ahmed, Cindy L McKenzie, Lance S Osborne

{"title":"Distribution of Scirtothrips dorsalis (Thysanoptera: Thripidae) cryptic species complex in the United States and reproductive host assessment of its dominant member.","authors":"Vivek Kumar, Yingfang Xiao, Matthew A Borden, Muhammad Z Ahmed, Cindy L McKenzie, Lance S Osborne","doi":"10.1093/jee/toad138","DOIUrl":"10.1093/jee/toad138","url":null,"abstract":"Chilli thrips, Scirtothrips dorsalis Hood, is a cryptic species complex of at least 9 species, 2 of which (South Asia 1 and East Asia 1) have been confirmed damaging >50 plant taxa in the United States. To develop a knowledge-based management program for this pest in the United States, the objectives of the study were to (i) survey for S. dorsalis species complex in the suspected regions and (ii) assess the reproductive hosts of the predominant member available. Thrips samples received from collaborators were subjected to morpholo-molecular characterization. Of the 101 thrips populations received across the United States between 2015 and 2021, 71 populations were confirmed as S. dorsalis, with ~25% further identified as East Asia 1, compared with ~41% identified as South Asia 1, suggesting the latter species is more prevalent in the US. East Asia 1 was primarily found in samples collected from Hydrangea sp. (95%) in Massachusetts, New York, and Pennsylvania, indicating geographic range expansion in the Northeast and successful overwintering in areas with hard freezes. While assessing the host range of South Asia 1 (dominant species), 62 plant taxa were evaluated under greenhouse conditions. Among these, 40 feeding and reproductive hosts were confirmed in Florida. We also found 18 new reproductive hosts and 3 feeding hosts of S. dorsalis, which were not previously reported in the literature. The results provide insight into the increasing host range and expanded geographical distribution of S. dorsalis species and will enable the development of a species-specific monitoring and management program.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":" ","pages":"1715-1726"},"PeriodicalIF":2.2,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10214082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characterization of Bombyx mori (Lepidoptera: Bombycidae) C-type lectin 5. 家蚕C型凝集素5。

IF 2.5 2区农林科学

Journal of Economic Entomology Pub Date : 2023-10-10 DOI: 10.1093/jee/toad142

Yan-Xia Sun, Bang-Xian Zhang, Fang-Fang Liu, Xiang-Jun Rao

{"title":"Functional characterization of Bombyx mori (Lepidoptera: Bombycidae) C-type lectin 5.","authors":"Yan-Xia Sun, Bang-Xian Zhang, Fang-Fang Liu, Xiang-Jun Rao","doi":"10.1093/jee/toad142","DOIUrl":"10.1093/jee/toad142","url":null,"abstract":"C-type lectins (CTLs) are an important family of pattern recognition receptors (PRRs) that regulate immune responses. The CTL5 gene of the silkworm Bombyx mori L. (Lepidoptera: Bombycidae) encodes a protein comprised of 223 amino acids, containing a signal peptide and a carbohydrate recognition domain (CRD). Our previous study showed that CTL5 can facilitate the clearance of bacteria from larval hemocoel but the underlying mechanisms are unclear. In this study, we found that CTL5 was mainly expressed in fourth-instar larvae, adult moths, and the larval epidermis. CTL5 expression showed differential responses to both pathogenic stimuli and the molting hormone 20-hydroxyecdysone. The full-length (FL) and truncated (ΔN/ΔC/ΔNC) CTL5 recombinant proteins can bind to hemocytes, polysaccharides, bacteria, and spores of the entomopathogenic fungus Beauveria bassiana. Yeast 2-hybrid assays showed that the recombinant proteins can interact with integrin β2-β5 subunits. Recombinant proteins increased the phagocytic rate of hemocytes. Injection of recombinant CTL5 stimulated the expression of many immune genes in hemocytes, mainly antimicrobial peptides and immune signaling molecules. Additionally, transcriptomic sequencing of CTL5-stimulated hemocytes revealed 265 upregulated and 580 downregulated genes. Functional enrichment and the gene set enrichment analyses showed that differentially expressed genes were mainly enriched in innate immune responses and signaling. Our study suggests that CTL5 may act as an opsonin to enhance the clearance of pathogens by regulating both humoral and cellular responses.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":" ","pages":"1862-1875"},"PeriodicalIF":2.5,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10296176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal density and natural mortality of Halyomorpha halys (Stål) and indigenous stink bugs (Hemiptera: Pentatomidae) in a field crop agroecosystem. 田间作物农业生态系统中Halyomorpha halys（Stål）和本土蝽（半翅目：蝽科）的季节密度和自然死亡率。

IF 2.5 2区农林科学

Journal of Economic Entomology Pub Date : 2023-10-10 DOI: 10.1093/jee/toad159

P Glynn Tillman, Erin E Grabarczyk, Katelyn A Kesheimer, Rhammohan Balusu

{"title":"Seasonal density and natural mortality of Halyomorpha halys (Stål) and indigenous stink bugs (Hemiptera: Pentatomidae) in a field crop agroecosystem.","authors":"P Glynn Tillman, Erin E Grabarczyk, Katelyn A Kesheimer, Rhammohan Balusu","doi":"10.1093/jee/toad159","DOIUrl":"10.1093/jee/toad159","url":null,"abstract":"Stink bugs (Hemiptera: Pentatomidae), including the exotic Halyomorpha halys (Stål), Nezara viridula (L.), and other indigenous species, are pests that damage a variety of agricultural crops. At a study site in the southeastern United States, we measured the density of stink bug species and patterns of parasitism and predation on corn, cotton, and soybean and host trees in an adjacent woodline. We assessed parasitism and predation of naturally laid egg masses in crops and sentinel egg masses in host trees and used pheromone-baited traps to determine H. halys seasonal development. Overall, H. halys and N. viridula were the dominant bugs observed. Adult H. halys were first detected each year on trees, followed by corn, and then cotton and soybean, suggesting that trees served as a source of H. halys dispersing into crops. For H. halys, more nymphs were captured in soybean than in corn or cotton. For N. viridula, more adults were captured in corn and cotton than in soybean, and more nymphs were captured in corn during 2019 and 2021 than in 2020. Percentage parasitism of N. viridula egg masses (74.2%) was higher than than that for H. halys egg masses (54.3%). Accordingly, conservation biological control has the potential to enhance parasitism of indigenous stink bugs and H. halys in field crop agroecosystems.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":" ","pages":"1636-1648"},"PeriodicalIF":2.5,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9965760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of cold storage after cold acclimation on the fitness of Habrobracon hebetor (Hymenoptera: Braconidae). 冷驯化后冷藏对哈氏茧蜂适应度的影响（膜翅目：茧蜂科）。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-10-10 DOI: 10.1093/jee/toad134

Lan Wei, Mao-Fa Yang, Ning Huang, Hou-Ding Ou, Xiu-Qin Wang, Yao Huang, Xiao-Fei Yu

{"title":"Effects of cold storage after cold acclimation on the fitness of Habrobracon hebetor (Hymenoptera: Braconidae).","authors":"Lan Wei, Mao-Fa Yang, Ning Huang, Hou-Ding Ou, Xiu-Qin Wang, Yao Huang, Xiao-Fei Yu","doi":"10.1093/jee/toad134","DOIUrl":"10.1093/jee/toad134","url":null,"abstract":"Habrobracon hebetor (Say) (Hymenoptera: Braconidae) is a cosmopolitan, idiobiont, and gregarious ectoparasitoid, which can parasitize the larvae of several pyralid and noctuid moths. However, adult parasitoids require cold storage to ensure that adequate individuals are available when a pest outbreak occurs. To understand the effects of cold storage after acclimation on offspring fitness of H. hebetor, the development, fecundity, population parameters, and paralysis rate of the F1 generation were evaluated using an age-stage, two-sex life table. Four pairing treatments were used in this study, with refrigerated males and females (ReF×ReM), unrefrigerated females and refrigerated males (UnF×ReM), refrigerated females and unrefrigerated males (ReF×UnM), and unrefrigerated females and males (UnF×UnM, control). Cold storage after acclimation had no significant effect on the fecundity or oviposition period of F0-generation H. hebetor. Moreover, the survival rate (Sa = 61.43%), proportion of females (Nf/N = 0.41), intrinsic rate of increase (r = 0.3450), finite rate of increase (λ = 1.4121), net reproduction rate (R0 = 149.47), and net paralysis rate (C0 = 74.52) of ReF×UnM and UnF×UnM (Sa = 50.00%, Nf/N = 0.34, r = 0.3297, λ = 1.3881, R0 = 155.69, C0 = 62.90, respectively) treatments were significantly higher than those of the ReF×ReM treatment (Sa = 45%, Nf/N = 0.16, r = 0.2277, λ = 1.2558, R0 = 68.81, C0 = 31.61, respectively) (except for the Sa of UnF×UnM treatment), and there was no significant difference between the 2 treatments. Overall, it is advisable to avoid simultaneous cold storage of female and male parasitoids or to add unrefrigerated males appropriately when using cold-stored parasitoids to control pests.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":" ","pages":"1496-1504"},"PeriodicalIF":2.2,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9843621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systemic insecticides for control of stem gall wasp in highbush blueberry. 系统杀虫剂防治高山越橘茎胆蜂。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-10-10 DOI: 10.1093/jee/toad162

Amber K DeVisser, Christine Vandervoort, Rufus Isaacs, John Wise

{"title":"Systemic insecticides for control of stem gall wasp in highbush blueberry.","authors":"Amber K DeVisser, Christine Vandervoort, Rufus Isaacs, John Wise","doi":"10.1093/jee/toad162","DOIUrl":"10.1093/jee/toad162","url":null,"abstract":"The gall wasp, Hemadas nubilipennis Ashmead, is a pest of highbush and lowbush blueberry and can pose a challenge to control with foliar sprays due to adult activity being during bloom and because larval development is within plant tissues. We hypothesized that systemic insecticides that move within the blueberry vascular system would reach areas where H. nubilipennis eggs are laid, causing larval mortality. Three application methods, crown injection, soil drench, and foliar spray were applied to potted 'Jersey' blueberry bushes at 50% and 100% rates to quantify systemic residue concentrations in shoots and leaves. Additionally, systemic insecticides were evaluated for control of gall wasps using single-shoot bioassays and measuring larval mortality at 0.01%, 0.1%, 1%, and 10% of field rate provided within a floral pick. Systemic insecticides tested in both studies included imidacloprid, flupyradifurone, and spirotetramat. The potted bush residue study determined that insecticides moved from three tested sites of entry: the roots, crown cavity, and foliage. Results from the shoot bioassays found that the mean percent larval survival of H. nubilipennis was negatively correlated with the concentration of AI detected in galls. Imidacloprid and spirotetramat were found to have the greatest potential for control of H. nubilipennis due to mortality in the shoot bioassays and similar residue concentrations in the potted bush studies to shoot bioassays. Future research should evaluate systemic insecticides applied in highbush blueberry plantings for control of H. nubilipennis using the bioassay mortality assessment method developed in this study.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":" ","pages":"1737-1749"},"PeriodicalIF":2.2,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d1/db/toad162.PMC10564264.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10030180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimating plume reach and trapping radii for male and female Cydia pomonella (Lepidoptera: Tortricidae) captured in pheromone-kairomone baited traps in Washington State apple orchards under mating disruption. 在交配中断的情况下，估计华盛顿州苹果园中用信息素kairomone诱饵诱捕器捕获的雄性和雌性小绒球（鳞翅目：小绒球科）的羽流范围和诱捕半径。

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-10-10 DOI: 10.1093/jee/toad167

R T Curtiss, Louis Nottingham, Larry J Gut

{"title":"Estimating plume reach and trapping radii for male and female Cydia pomonella (Lepidoptera: Tortricidae) captured in pheromone-kairomone baited traps in Washington State apple orchards under mating disruption.","authors":"R T Curtiss, Louis Nottingham, Larry J Gut","doi":"10.1093/jee/toad167","DOIUrl":"10.1093/jee/toad167","url":null,"abstract":"Male Cydia pomonella (L.) (Lepidoptera: Tortricidae) dispersion has largely been studied in nonmating disrupted orchards due to synthetic pheromone interference with capture in monitoring traps. Little is known about female dispersion. This study aimed to characterize male and female dispersion in mating disrupted commercial apple orchards. Sterile C. pomonella recapture data from single-trap multiple-release experiments using PHEROCON CM-DA COMBO + AA Lure-baited orange Pherocon VI delta traps was interpreted to determine pheromone-kairomone lure-baited trap effective area, trap deployment density for effective monitoring, and absolute male and female C. pomonella density in mating disrupted Washington commercial apple orchards. The maximum plume reach of the pheromone-kairomone lure in mating disrupted orchards was <5 m from the baited trap for both sexes. Maximum dispersive distances for 95% of the released C. pomonella in mating disrupted orchards were 106 and 135 m for males and females, yielding trapping areas of 3.87 and 6.16 ha, respectively. Estimates were consistent across 3 growing seasons and represent the first records of male and female dispersal distance and monitoring trap efficacy from commercial C. pomonella mating disrupted apple orchards. With relevance to commercial monitoring programs and economic thresholds in mating disrupted orchards, traps should be deployed at a density of 1 per 3-6 ha. Capture of a single male or female C. pomonella corresponds to at least 82-104 C. pomonella within the 3-6 ha trapping area. This refined C. pomonella capture interpretation in pheromone-kairomone baited traps in mating disrupted commercial apple orchards yields more precise damage estimates and assists in insecticide-use decision making.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":" ","pages":"1592-1603"},"PeriodicalIF":2.2,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10057294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sampling Lasioderma serricorne (Coleoptera: Anobiidae) in 2 coffee bean warehouses in New Jersey. 在新泽西州的两个咖啡豆仓库中对细尾蠊（鞘翅目：Anobidae）进行采样。

IF 2.5 2区农林科学

Journal of Economic Entomology Pub Date : 2023-10-10 DOI: 10.1093/jee/toad131

Xiaodan Pan, Changlu Wang

{"title":"Sampling Lasioderma serricorne (Coleoptera: Anobiidae) in 2 coffee bean warehouses in New Jersey.","authors":"Xiaodan Pan, Changlu Wang","doi":"10.1093/jee/toad131","DOIUrl":"10.1093/jee/toad131","url":null,"abstract":"Cigarette beetle, Lasioderma serricorne (F.), is one of the most common stored-product pests. We monitored their population dynamics and distribution in two coffee bean warehouses in New Jersey, USA, using pheromone traps and sticky traps during September 2018-October 2020, and light traps in 2020. The two warehouses only implemented treatment procedures for controlling Indian meal moth (Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae)) during the study period. The first L. serricorne adult appeared on pheromone traps from late May to early June when temperature reached 21-22 °C, and the last L. serricorne adult appeared on pheromone traps from late October to mid-November when temperature dropped to 10-14 °C. The majority of L. serricorne was caught during July-October. Light traps caught 5.5- and 2.2-times more L. serricorne per trap than pheromone traps in Warehouse 1 and 2, respectively. Warehouse 1 had a significantly higher density of L. serricorne than Warehouse 2. The L. serricorne activity peaks were not always clear and varied between year and the two warehouses. Zero to 3 hot spots, where had the largest numbers of L. serricorne, were identified from July to October in each warehouse based on pheromone traps, and their locations were similar through the months both in 2019 and 2020. The L. serricorne counts from pheromone traps placed inside warehouse were at least 2.3-times more than those placed outside. Also, the L. serricorne active period outside of the warehouses was shorter than that from inside of the warehouses.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":" ","pages":"1902-1910"},"PeriodicalIF":2.5,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9768612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A high-throughput plate method for nucleic acid extraction from beet leafhopper (Hemiptera: Cicadellidae) and potato psyllid (Hemiptera: Triozidae) for pathogen detection. 一种从甜菜叶蝉（半翅目：蝉科）和马铃薯木虱（半翅目：Triozidae）中提取核酸用于病原体检测的高通量平板法。

IF 2.5 2区农林科学

Journal of Economic Entomology Pub Date : 2023-10-10 DOI: 10.1093/jee/toad153

Christopher J Gorman, David W Crowder, Kylie D Swisher Grimm

{"title":"A high-throughput plate method for nucleic acid extraction from beet leafhopper (Hemiptera: Cicadellidae) and potato psyllid (Hemiptera: Triozidae) for pathogen detection.","authors":"Christopher J Gorman, David W Crowder, Kylie D Swisher Grimm","doi":"10.1093/jee/toad153","DOIUrl":"10.1093/jee/toad153","url":null,"abstract":"Plant pathogens that are transmitted by insect vectors cause considerable damage to crops when pests or pathogens are not detected early in the season and populations are not controlled. Knowledge of pathogen prevalence in insect pest populations can aid growers in their insect pest management decisions but requires the timely dissemination of results. This process requires that specimen capture, identification, nucleic acid extraction, and molecular detection of a pathogen(s) occur alongside a platform for sharing results. The potato psyllid (Bactericera cockerelli, Sulc; Hemiptera: Triozidae) and beet leafhopper (Circulifer tenellus, Baker; Hemiptera: Cicadellidae) transmit pathogens to potato and other vegetable or seed crops each season in the northwestern United States. While the potato psyllid has been tested for pathogen occurrence for the past decade, testing of the beet leafhopper is a new endeavor and substantially increases the specimen number that must be tested by our laboratories each season. To aid in the rapid processing of individual insect specimens, we optimized and validated a new high-throughput 96-well plate nucleic acid extraction method for use in place of a standard 1.5-ml single-tube extraction method. Processing efficiency, in terms of total specimens processed over a 2-day period, improved 2.5-fold, and the cost associated with processing a single sample was nearly cut in half with this newly developed plate nucleic acid extraction method. Overall, this method has proven to be an excellent tool for the rapid testing of large numbers of small, individual insect vectors to enable timely dissemination of data on pathogen prevalence to growers.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":" ","pages":"1876-1884"},"PeriodicalIF":2.5,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9997764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating global Vaccinium germplasm for resistance against invasive Drosophila suzukii (Diptera: Drosophilidae). 全球维蓝种质对入侵铃木果蝇的抗性评价(双翅目:果蝇科)

IF 2.2 2区农林科学

Journal of Economic Entomology Pub Date : 2023-08-10 DOI: 10.1093/jee/toad081

Blair Sampson, Stephen Stringer, Kim Hummer, Ebrahiem Babiker, Chris Werle, John Adamczyk, Donna Shaw

{"title":"Evaluating global Vaccinium germplasm for resistance against invasive Drosophila suzukii (Diptera: Drosophilidae).","authors":"Blair Sampson, Stephen Stringer, Kim Hummer, Ebrahiem Babiker, Chris Werle, John Adamczyk, Donna Shaw","doi":"10.1093/jee/toad081","DOIUrl":"https://doi.org/10.1093/jee/toad081","url":null,"abstract":"Control of spotted-wing Drosophila, Drosophila suzukii, in small fruits emphasizes biological, cultural, and chemical approaches, whereas studies of host plant resistance as a form of genetic control are just getting underway. The identification of resistance patterns among genotypes of host plants whose fruit, leaves, roots, stems, or seeds are specifically targeted by an invasive pest is the first step in the development of an effective genetic control. Therefore, a detached fruit bioassay was developed to screen for D. suzukii oviposition and larval infestation within berries from 25 representative species and hybrids of wild and cultivated Vaccinium. Ten Vaccinium species showed strong resistance; among them, two wild diploids originating from within the fly's native range: V. myrtoides and V. bracteatum. Other resistant species came from the sections Pyxothamnus and Conchophyllum. They included New World V. consanguineum and V. floribundum. Large-cluster blueberry, V. amoenum, and three Floridian genotypes of related rabbiteye blueberry, V. virgatum, were the only hexaploids expressing strong resistance against D. suzukii. Most screened blueberry genotypes from managed lowbush and cultivated highbush types were susceptible to the flies' attacks (i.e., oviposition). Tetraploid blueberries tended to host the most eggs, whereas diploids and hexaploids harbored 50%-60% fewer eggs, on average. D. suzukii cannot lay eggs or complete development in the smallest, sweetest, and firmest diploid fruits. Likewise, certain genotypes of large-fruited tetraploid and hexaploid blueberry strongly curbed D. suzukii egg-laying and larval growth, indicating the possibility of heritable resistance operating against this invasive fly species.","PeriodicalId":15632,"journal":{"name":"Journal of Economic Entomology","volume":"116 4","pages":"1398-1410"},"PeriodicalIF":2.2,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10356424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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