Molecular Imprinting最新文献_第2页

ATP-binding peptide-hydrogel composite synthesized by molecular imprinting on beads 分子印迹法合成atp结合肽-水凝胶复合物

Molecular Imprinting Pub Date : 2015-12-31 DOI: 10.1515/molim-2015-0008

Aya Takata, K. Usui, J. Matsui

引用次数: 2

Analysis of cooperative interactions in molecularly imprinted polymer nanoparticles 分子印迹纳米聚合物中协同相互作用的分析

Molecular Imprinting Pub Date : 2015-12-31 DOI: 10.1515/molim-2015-0003

J. Mistry, A. Guerreiro, E. Moczko, E. Piletska, K. Karim, S. Piletsky

{"title":"Analysis of cooperative interactions in molecularly imprinted polymer nanoparticles","authors":"J. Mistry, A. Guerreiro, E. Moczko, E. Piletska, K. Karim, S. Piletsky","doi":"10.1515/molim-2015-0003","DOIUrl":"https://doi.org/10.1515/molim-2015-0003","url":null,"abstract":"Abstract Cooperative binding is commonly observed in biological receptor systems. This study investigates whether it is possible to prepare nano-sized molecularly imprinted polymers (nanoMIPs) that show cooperative binding. NanoMIPs which exhibit cooperative binding would have increased affinity for immobilised template molecules making them useful for advanced applications in diagnostics and sensors. The use of a templatederivatised solid support provides a facile route to prepare nanoMIPs with surface imprints, and the method is ideally suited to study this topic. Although not observed during the course of this study, positive interbinding site cooperativity was hypothesised by way of an increase in the number of binding sites imprinted on the nanoMIPs, by increasing template density on the solid support surface. After synthesis, the affinity of nanoMIPs was analysed using surface plasmon resonance (SPR) technique. Under the conditions investigated, a ten fold increase in binding affinity was measured as template density was increased. SPR results could be explained by an increase in cooperative binding; however calculations showed that the increase in affinity was not significant enough to prove cooperative binding interactions. The main conclusion obtained was that MIP nanoparticles contain only one “high-affinity” binding site that interacts with immobilised template in an SPR assay.","PeriodicalId":155658,"journal":{"name":"Molecular Imprinting","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127855781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Improved molecularly imprinted polymer grafted to porous polyethylene frits for the solid-phase extraction of thiabendazole from citrus sample extracts 多孔聚乙烯接枝分子印迹聚合物固相萃取柑橘样品提取物中噻苯达唑

Molecular Imprinting Pub Date : 2015-05-27 DOI: 10.1515/molim-2015-0001

M. Díaz-Álvarez, E. Turiel

引用次数: 5

Molecular Imprinting for proteins and related biomolecules – preface 蛋白质和相关生物分子的分子印迹。前言

Molecular Imprinting Pub Date : 2015-01-31 DOI: 10.1515/MOLIM-2015-0009

T. Takeuchi

引用次数: 1

Selective determination of penicillin G from tap water and milk samples using surface molecularly imprinted polymers as solid-phase extraction sorbent 用表面分子印迹聚合物作为固相萃取吸附剂选择性测定自来水和牛奶样品中的青霉素G

Molecular Imprinting Pub Date : 2014-10-21 DOI: 10.2478/molim-2014-0002

Wei Du, Huiyan Zhou, Zhimin Luo, Penglei Zheng, Pengqi Guo, Ruimiao Chang, Chunran Chang, Qiang Fu

{"title":"Selective determination of penicillin G from tap water and milk samples using surface molecularly imprinted polymers as solid-phase extraction sorbent","authors":"Wei Du, Huiyan Zhou, Zhimin Luo, Penglei Zheng, Pengqi Guo, Ruimiao Chang, Chunran Chang, Qiang Fu","doi":"10.2478/molim-2014-0002","DOIUrl":"https://doi.org/10.2478/molim-2014-0002","url":null,"abstract":"Abstract In this study, a selective sample preparation method coupling surface molecularly imprinting polymers (SMIPs) with solid-phase extraction (SPE) was developed for the determination of penicillin G from tap water and milk samples. SMIPs for penicillin G were synthesized by using silica gel as supporting matrix, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EDMA) as the cross-linker, methanolacetonitrile (1:1,v/v) as the solvents and 2,2′-azo-bisisobutyronitrile (AIBN) as the initiator. Characterization and adsorption experiments revealed that SMIPs exhibited large adsorption capacity, high recognition ability and high rate of mass transfer. Application of SMIPs in SPE followed by HPLC was done to selectively determine penicillin G from tap water and milk samples. Under the optimal conditions, the proposed method demonstrated high linearity with the concentration of penicillin G ranging from 0.12 to 200 μg mL-1. The limits of detection (LOD) in tap water and milk were 0.05, 0.03μg mL-1, respectively. The limits of quantification (LOQ) in tap water and milk were 0.25, 0.12 μg mL-1, respectively. The recoveries of penicillin G at three spiked levels were ranged from 78.9 to 85.5 % with intra-day and inter-day relative standard deviation values below 4.8 %.","PeriodicalId":155658,"journal":{"name":"Molecular Imprinting","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123507251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Molecular Imprinting 分子印迹

Molecular Imprinting Pub Date : 1900-01-01 DOI: 10.1007/978-3-642-28421-2

K. Mosbach

引用次数: 270

Molecularly imprinted microspheres prepared by precipitation polymerization at high monomer concentrations 高单体浓度沉淀聚合制备分子印迹微球

Molecular Imprinting Pub Date : 1900-01-01 DOI: 10.2478/molim-2014-0001

Tibor Renkecz, K. László, V. Horváth

引用次数: 26