Nafisah Nafisah, Sarmila Sarmila, Hanna Habibah, Indah Saputri, Indah Setiawati, N. Komari
{"title":"Effect of Kelakai (Stenochlaena palutris) Extract on Organophosphate Pesticide Exposure: Cytotoxic Studies in Ovo and in Silico","authors":"Nafisah Nafisah, Sarmila Sarmila, Hanna Habibah, Indah Saputri, Indah Setiawati, N. Komari","doi":"10.20527/jstk.v17i2.14583","DOIUrl":"https://doi.org/10.20527/jstk.v17i2.14583","url":null,"abstract":"Vegetable farmers in South Kalimantan use pesticides to protect crops from pests. The active ingredient of the pesticide has a broad toxic effect on target and non-target organisms. Continuous exposure to pesticides causes cancer. The plant of mantle (Stenochlaena palustris) is thought to have potential cytotoxic activity against cancer cell growth. The aim of this study was to examine the potential activity of chemical compounds in anaplastic lymphoma kinase (ALK) exposed to organophosphate pesticides in silico and in ovo. The in silico study used molecular docking and virtual screening methods. The methanol extract of malachite was obtained by maceration method. The in ovo study was carried out by injecting pesticide compounds and methanol extracts of methanol at doses of 0.5 and 1 ppm to free-range chicken eggs. The results of the in silico analysis showed that ethion and neophytadiene had the most negative G values, namely -8.62 kcal/mol and -8.39 kcal/mol, while the natural ligand 8 LY A 500 was -9.19 kcal/mol. The similarity of the type and number of residues in the binding complex between ethion and neophyadiene ligands with ALK protein indicates the potential for competition between ethion and neophyadiene when bound to ALK protein. Neofitadiene is suspected as a compound that has potential as anti-cancer by inhibiting the growth of ALK. Anchovy extract is considered to be able to slow down the rate of cell damage in chicken embryos caused by ethion with its inhibitory ability so that the cell surface is not damaged quickly.","PeriodicalId":155441,"journal":{"name":"Jurnal Berkala Ilmiah Sains dan Terapan Kimia","volume":"98 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139315431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. S. Asmawati, Rahmi Rahmi, Desi Reski Fajar, Ira Widya Sari
{"title":"Evaluation of Antioxidant Activity in Lemon Juice (Citrus limon) Marketed in Makassar City Using the DPPH (2,2 diphenyl-1-picrylhydrazyl) Method","authors":"A. S. Asmawati, Rahmi Rahmi, Desi Reski Fajar, Ira Widya Sari","doi":"10.20527/jstk.v17i2.14903","DOIUrl":"https://doi.org/10.20527/jstk.v17i2.14903","url":null,"abstract":"Human lifestyle is currently experiencing many changes that lead to unhealthy lifestyles. An instant lifestyle can trigger degenerative diseases, which are initiated by excessive oxidation reactions in the body, which can cause the formation of free radicals. Lemon juice, derived from the Citrus limon plant, is considered a functional beverage due to its antioxidant properties, which have the potential to counteract the harmful effects of free radicals. This study aimed to measure the antioxidant activity of lemon juice beverage based on the IC50 value. The present study adopted a descriptive laboratory approach, utilizing lemon juice beverages from three distinct brands as representative samples. The antioxidant activity test was carried out by adding DPPH and measured using UV-Vis Spectrophotometry. The antioxidant levels obtained were 1,091.17 µg/mL for sample A, 1,114.50 µg/mL for sample B, and 527,261 µg/mL for sample C. All samples exhibited antioxidant activity values that fell within the \"very weak\" classification, as shown by IC50 values above 200 µg/mL.","PeriodicalId":155441,"journal":{"name":"Jurnal Berkala Ilmiah Sains dan Terapan Kimia","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139315006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dominikus Niholan Tukan, L. Rosmainar, Kustomo Kustomo, Rasidah Rasidah
{"title":"A Review: Optimum Conditions for Magnetite Synthesis (Fe3O4)","authors":"Dominikus Niholan Tukan, L. Rosmainar, Kustomo Kustomo, Rasidah Rasidah","doi":"10.20527/jstk.v17i2.15134","DOIUrl":"https://doi.org/10.20527/jstk.v17i2.15134","url":null,"abstract":"Magnetite, chemically represented as Fe3O4, is an opaque substance characterized by its black coloration. It possesses a significant saturation magnetization value, denoting a substantial magnetic strength compared to alternative materials. Magnetite (Fe3O4) has significant use across several domains, including its deployment as a catalyst and sensor in the medical sector, such as drug delivery, hyperthermia therapy, and magnetic resonance imaging (MRI). The synthesis of magnetite (Fe3O4) can be achieved using several methodologies, such as solvothermal, sol-gel, solid state, reverse micelle, microwave plasma synthesis, freeze drying, ultrasound irradiation, hydrothermal, and coprecipitation techniques. The co-preparation technique is extensively employed for magnetite synthesis due to its simplicity, ease of usage, and ability to generate magnetic particles at the nanoscale scale without elevated temperatures. The outcome of the coprecipitation process is influenced by various factors such as the nature of the salt, molar ratio, pH level, stirring rate, and reaction temperature. This article aims to examine the impact of many factors, including the salt type, molar ratio, pH level, stirring rate, and reaction temperature, on the production of Magnetite (Fe3O4) through coprecipitation. It was found that Magnetite (Fe3O4) synthesis can produce nanoparticle size material with great magnetic power, namely at pH 8, temperature 70°C, and stirring rate of 10,000 rpm by using FeCl3 and FeCl2 as precursors Fe3+ and Fe2+ with a ratio of 1.5:1 and using precipitating base NH4OH.","PeriodicalId":155441,"journal":{"name":"Jurnal Berkala Ilmiah Sains dan Terapan Kimia","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139315163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Della Puspita Indriyani, V. Fabiani, Fajar Indah Puspita Sari
{"title":"Synthesis of Nano Silver From Melastoma malabathricum Leaf Extracts Modified PVA and its Antibacterial Activity Test","authors":"Della Puspita Indriyani, V. Fabiani, Fajar Indah Puspita Sari","doi":"10.20527/jstk.v17i2.13862","DOIUrl":"https://doi.org/10.20527/jstk.v17i2.13862","url":null,"abstract":"Synthesis of silver nanoparticles from the extract of senduduk leaf (Melastoma malabathricum) with modified polyvinyl alcohol (PVA) has been carried out. The purpose of this study was to determine the effect of the addition of PVA on the characteristics of silver nanoparticles formed using the extract of sensaat leaf (Melastoma malabathricum). Variations in the concentration of PVA used were 0%; 0.75%; 1.5%; and 3%. Synthesis was carried out in a ratio of 1:10:1 (Extract: AgNO3: PVA) for 2 hours. The characteristics of the synthesis of nanoparticles using a UV-Vis spectrophotometer resulted in a consecutive wavelength of 414 nm; 435 nm; 438 nm; and 420 nm. To determine the particle size using Particle Size Analyzer (PSA) analysis at 0% PVA and 3% PVA with sizes obtained 61.9 nm and 216.2 nm. The average particle size using X-Ray Diffraction (XRD) analysis showed the results in the form of crystals and using the Debye-Scherrer equation to obtain the average particle size of PVA 0% 22.97 nm and PVA 3% 10.15 nm. Antibacterial activity test on silver nanoparticles used disc diffusion method, which showed that silver nanoparticles had moderate to strong antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria.Keywords: Bioreductor, senduduk leaf extract, silver nanoparticles, and polyvinyl alcohol","PeriodicalId":155441,"journal":{"name":"Jurnal Berkala Ilmiah Sains dan Terapan Kimia","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139314946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Retno Agnestisia, K. Karelius, R. M. Iqbal, Dyah Ayu Pramoda Wardani, Midun Efendi Patar Sihombing, Sri Yulandari Simangunsong, Junita Dongoran
{"title":"Utilization of Ferrate Ion (FeO42-) as Oxidizing Agent for Reducing Color Intensity of Peat Water","authors":"Retno Agnestisia, K. Karelius, R. M. Iqbal, Dyah Ayu Pramoda Wardani, Midun Efendi Patar Sihombing, Sri Yulandari Simangunsong, Junita Dongoran","doi":"10.20527/jstk.v17i2.14832","DOIUrl":"https://doi.org/10.20527/jstk.v17i2.14832","url":null,"abstract":"Peat water from Central Kalimantan is generally yellow to brownish-red in color. It causes peat water to not be used as a source of water for daily needs. Humic acid, fulvic acid, and humin are organic compounds that cause peat water to become colored. One alternative step that can be taken for reducing the color intensity of peat water is to degrade color-causing organic compounds by using an oxidizing agent. A hexavalent form of iron ion (Fe6+), commonly called ferrate (FeO42-) in potassium ferrate compound, is a powerful oxidizing agent used in the present study. Potassium ferrate (K2FeO4) was synthesized by a wet oxidation method and characterized using a UV-Vis spectrophotometer and an X-Ray Diffractometer (XRD). The ferrate solution is purplish-red in color with a maximum wavelength of 510 nm. The XRD pattern shows strong diffraction peaks at 2θ which are characteristic of K2FeO4. The ferrate solution was then used to degrade color-causing organic compounds in the peat water from Central Kalimantan. Determination of the optimum degradation conditions was monitored by the absorbance decrease of organic compounds in peat water at a wavelength of 370 nm. The results showed that ferrate solution with a concentration of 80 ppm was able to degrade organic compounds in peat water at pH 8, with a removal efficiency of 100%. Keywords: oxidizing agent, ferrate ion (FeO42-), peat water","PeriodicalId":155441,"journal":{"name":"Jurnal Berkala Ilmiah Sains dan Terapan Kimia","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139315200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nova Ellya Widiyati, Iffah Muflihati, Rini Umiyati, A. R. Affandi
{"title":"Pretreatment of Aloe Vera and Its Effect on the Properties of Flour and Vermicelli Produced","authors":"Nova Ellya Widiyati, Iffah Muflihati, Rini Umiyati, A. R. Affandi","doi":"10.20527/jstk.v17i2.14900","DOIUrl":"https://doi.org/10.20527/jstk.v17i2.14900","url":null,"abstract":"Aloe vera powder has a less bright color due to the browning reaction. Pretreatment was carried out to improve the quality of aloe vera flour. Aloe vera flour can be used to manufacture food products, one of which is vermicelli. This study aimed to determine the effect of different pretreatments, namely blanching, sodium metabisulfite immersion, sodium bisulfite immersion, and steam blanching, on the characteristics of aloe vera flour and its application to vermicelli. The research steps included aloe vera pretreatment and aloe vera flour production, vermicelli production with aloe vera flour substitution, and analysis. The results showed that the pretreatment produced aloe vera and vermicelli flour with a brighter color. Pretreatment also affects water content, water-holding capacity, oil-holding capacity, and cooking properties. But the pretreatment did not affect the sensory properties of vermicelli.","PeriodicalId":155441,"journal":{"name":"Jurnal Berkala Ilmiah Sains dan Terapan Kimia","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139315291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nila Tanyela Berghuis, Ana Lailatul Farida, A. Ilmiawati
{"title":"Qualitative and Quantitative Characterization of Secondary Metabolites of Qust' Al-Hindi (Saussurea Lappa) Plants","authors":"Nila Tanyela Berghuis, Ana Lailatul Farida, A. Ilmiawati","doi":"10.20527/jstk.v17i2.14831","DOIUrl":"https://doi.org/10.20527/jstk.v17i2.14831","url":null,"abstract":"Utilization of plants that have the potential as herbal medicines, including the Qust' Al-Hindi (Saussurea Lappa) plant. Identification of the secondary metabolite content of the Qust' Al-Hindi Plant (Saussurea Lappa) through Phytochemical analysis in the form of flavonoid, alkaloid, steroid, terpenoid, tannin, and saponin tests on the extraction results as well as the calculation of flavonoid levels which are the largest content of this plant carried out using the UV method. -Vis. The characterization of the presence of a class of secondary metabolites contained in the plant was analyzed using FTIR-ATR spectroscopy. The results of this study indicate that the plant contains secondary metabolites in the form of flavonoid compounds, alkaloids, terpenoids, and tannins. The flavonoid content obtained was 223.33 ± 66.5 mgQE/g, while the results of the FTIR-ATR test showed that the extract contained the same functional groups as flavonoids, alkaloids, terpenoids, and tannins, namely the O-H, C-H, C=C functional groups. C=O and C-O contained in the structure of flavonoids, alkaloid compounds are known to have a distinctive functional group, namely N-H, for terpenoids to have a distinctive functional group, namely C-H, namely CH2 and CH3, there is also CH and there is a C-O group which is a typical functional group of compounds tannins.","PeriodicalId":155441,"journal":{"name":"Jurnal Berkala Ilmiah Sains dan Terapan Kimia","volume":"22 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139315382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Giovanny Robertho Wolfram Lake, G. D. Gelyaman, S. M. Kolo
{"title":"Briquette from Candlenut Shell Charcoal and Polypropylene Plastic Waste From The Kefamenanu Landfill","authors":"Giovanny Robertho Wolfram Lake, G. D. Gelyaman, S. M. Kolo","doi":"10.20527/jstk.v17i2.13738","DOIUrl":"https://doi.org/10.20527/jstk.v17i2.13738","url":null,"abstract":"This research aimed to determine the optimum composition of polypropylene plastic and candlenut shell charcoal in briquettes production. The percentage of briquette mixture was CK100:0PP, CK75:25PP, CK50:50PP, CK25:75PP and CK0:100PP. The moisture content of the briquettes obtained was as follows CK100:0PP (6.33%), CK75:25PP (4.18%), CK50:50PP (2.63%), CK25:75PP (1.90%) and CK0: 100PP (0.77%). Ash content was CK100:0PP (0.51%), CK75:25PP (18.82%), CK50:50PP (9.09%), CK25:75PP (3.26%) and CK0:100PP (1.74%). The level of flying substances was CK100:0PP (33.53%), CK75:25PP (51.52%), CK50:50PP (52.37%), CK25:75PP (60.99%) and CK0:100PP (72.96%). Fixed carbon was CK100:0PP (59.63%), CK75:25PP (25.48%), CK50:50PP (35.91%), CK25:75PP (33.85%) and CK0:100PP (24.52 %). Calorific value was CK100:0PP (2,902.31 cal/gr), CK75:25PP (9,292.45 cal/gr), CK50:50PP (8,155.53 cal/gr), CK25:75PP (9,094.69 cal/gr) and CK0:100PP (10,808.30 cal/gr). Compressive strength was CK75:25PP (50.31 Kgf/cm2), CK50:50PP (44.67 Kgf/cm2), CK25:75PP (36.11 Kgf/cm2) and CK0:100PP (33.85 Kgf/cm2). The optimum composition of briquettes from a mixture of polypropylene plastic and candlenut shell charcoal was CK25:75PP.","PeriodicalId":155441,"journal":{"name":"Jurnal Berkala Ilmiah Sains dan Terapan Kimia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139315209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Macro and Micro Mineral Composition of Haruan Fish (Channa striata) in Banjar District, South Kalimantan","authors":"R. Yunus, D. Ariyani, Muhammad Aisy","doi":"10.20527/jstk.v17i1.12563","DOIUrl":"https://doi.org/10.20527/jstk.v17i1.12563","url":null,"abstract":"","PeriodicalId":155441,"journal":{"name":"Jurnal Berkala Ilmiah Sains dan Terapan Kimia","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114866863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isnaini Isnaini, Rosinta Dewi Achmadiyah, Gelvia Awaeh, H. Khatimah, A. Yasmina
{"title":"Antioxidant and Antiproliferative Activities of Methanol Extract from Melaleuca cajuputi subsp. Cumingiana [Turcz.] Fruit","authors":"Isnaini Isnaini, Rosinta Dewi Achmadiyah, Gelvia Awaeh, H. Khatimah, A. Yasmina","doi":"10.20527/jstk.v17i1.13055","DOIUrl":"https://doi.org/10.20527/jstk.v17i1.13055","url":null,"abstract":"Melaleuca cajuputi subsp. Cumingiana [Turcz.] Barlow (M. cajuputi) is a plant that is easily found in Banjarmasin. M. cajuputi contains phytochemical compounds in the form of polyphenols including flavonoids, quinons, saponins, and alkaloids that are thought to have antioxidant and antiproliferation activities. The aim of this research was to find out analyze antioxidant and antiproliferation activity of M. cajuputi fruit methanol extract. The antioxidant activity was tested using the DPPH method. The activities were observed in IC50 and were measured using the UV-VIS spectrophotometer at a wavelength of 517 nm. To test the antiproliferation, true experimental with post-test was applied in this research. Animal used in this study were 30 mature zebras (length > 2.5 cm) which were grouped into 4, namely the negative control group (DMSO 0.05%), the methanol extract group of M. cajuputi with concentrations of 18.5 ppm, 37 ppm and 74 ppm. The bound variable in this study was antiproliferation activity in the tail of an amputated fish. Data analysis was measured by one-way ANOVA and Post-Hoc Tukey HSD tests. Phytochemical results obtained the presence of phenol compounds, cuinons, flavonoids, alkaloids, saponins, tannins, steroids and terpenoids. Methanol extract of Melaleuca cajuputi subsp Cumingiana [Turcz.] Barlow fruit was at IC50 of 15.50 ppm (95% CI 8.31- 32.72). The antiproliferation activity of zebrafish tails increased in the administration of fruit extract Melaleuca cajuputi subsp. Cumingiana [Turcz.] Barlow in consentration of 74 ppm (p<0.05), both on day 4 and day 8 of measurement when compared to negative controls. It can be concluded that methanol extract of Melaleuca cajuputi subsp Cumingiana [Turcz.] Barlow fruit has antiproliferative activity against the growth of amputated zebrafish tails.","PeriodicalId":155441,"journal":{"name":"Jurnal Berkala Ilmiah Sains dan Terapan Kimia","volume":"154 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131832827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}