Synthesis of Nano Silver From Melastoma malabathricum Leaf Extracts Modified PVA and its Antibacterial Activity Test

Jurnal Berkala Ilmiah Sains dan Terapan Kimia Pub Date : 2023-10-23 DOI:10.20527/jstk.v17i2.13862

Della Puspita Indriyani, V. Fabiani, Fajar Indah Puspita Sari

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Abstract

Synthesis of silver nanoparticles from the extract of senduduk leaf (Melastoma malabathricum) with modified polyvinyl alcohol (PVA) has been carried out. The purpose of this study was to determine the effect of the addition of PVA on the characteristics of silver nanoparticles formed using the extract of sensaat leaf (Melastoma malabathricum). Variations in the concentration of PVA used were 0%; 0.75%; 1.5%; and 3%. Synthesis was carried out in a ratio of 1:10:1 (Extract: AgNO3: PVA) for 2 hours. The characteristics of the synthesis of nanoparticles using a UV-Vis spectrophotometer resulted in a consecutive wavelength of 414 nm; 435 nm; 438 nm; and 420 nm. To determine the particle size using Particle Size Analyzer (PSA) analysis at 0% PVA and 3% PVA with sizes obtained 61.9 nm and 216.2 nm. The average particle size using X-Ray Diffraction (XRD) analysis showed the results in the form of crystals and using the Debye-Scherrer equation to obtain the average particle size of PVA 0% 22.97 nm and PVA 3% 10.15 nm. Antibacterial activity test on silver nanoparticles used disc diffusion method, which showed that silver nanoparticles had moderate to strong antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria.Keywords: Bioreductor, senduduk leaf extract, silver nanoparticles, and polyvinyl alcohol

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从改性 PVA 的 Melastoma malabathricum 叶提取物中合成纳米银及其抗菌活性测试

用改性聚乙烯醇（PVA）从森杜鹃叶（Melastoma malabathricum）提取物中合成了纳米银粒子。本研究的目的是确定添加 PVA 对使用森达叶（Melastoma malabathricum）提取物形成的银纳米粒子特性的影响。所使用的 PVA 浓度分别为 0%、0.75%、1.5% 和 3%。合成以 1:10:1 的比例（提取物：AgNO3：PVA）进行，时间为 2 小时。使用紫外可见分光光度计检测合成纳米粒子的特征，结果显示连续波长分别为 414 nm、435 nm、438 nm 和 420 nm。使用粒度分析仪（PSA）分析 0% PVA 和 3% PVA 的粒度，得到的粒度分别为 61.9 nm 和 216.2 nm。使用 X 射线衍射（XRD）分析平均粒度，结果显示其为晶体形式，并使用 Debye-Scherrer 公式得出 PVA 0% 的平均粒度为 22.97 nm，PVA 3% 的平均粒度为 10.15 nm。纳米银颗粒的抗菌活性测试采用了盘扩散法，结果表明纳米银颗粒对金黄色葡萄球菌和大肠杆菌具有中等到较强的抗菌活性：生物诱导剂森古杜克叶提取物纳米银颗粒和聚乙烯醇

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Jurnal Berkala Ilmiah Sains dan Terapan Kimia

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