Journal of chromatography最新文献_第4页

Peptide mapping of recombinant human interferon-gamma by reversed-phase liquid chromatography with on-line identification by thermospray mass spectrometry and UV absorption spectrometry. 利用反相液相色谱和热喷雾质谱及紫外吸收光谱在线鉴定技术对重组人干扰素γ进行肽段定位。

Journal of chromatography Pub Date : 1993-09-10 DOI: 10.1016/0021-9673(93)83318-m

R Legrand, J B Falconnet, D Prevost, B Schoot, P Devaux

引用次数: 4

Liquid chromatographic determination of para-substituted N,N-dialkylaniline N-oxides. 液相色谱法测定对取代N,N-二烷基苯胺N-氧化物。

Journal of chromatography Pub Date : 1993-09-08

Y Seto, F P Guengerich

引用次数: 0

Characterisation of the photochemotherapeutic agent disulphonated aluminium phthalocyanine and its high-performance liquid chromatographic separated components. 光化学治疗剂酞菁二磺化铝及其高效液相色谱分离组分的表征。

Journal of chromatography Pub Date : 1993-09-03 DOI: 10.1016/0021-9673(93)83347-u

S M Bishop, B J Khoo, A J MacRobert, M S Simpson, D Phillips, A Beeby

引用次数: 31

Study of lectin-ganglioside interactions by high-performance liquid affinity chromatography. 高效液相亲和色谱法研究凝集素-神经节苷脂相互作用。

Journal of chromatography Pub Date : 1993-09-03 DOI: 10.1016/0021-9673(93)83345-s

M Caron, R Joubert-Caron, J R Cartier, A Chadli, D Bladier

引用次数: 14

Use of a porous graphitised carbon column for the high-performance liquid chromatography of oligosaccharides, alditols and glycopeptides with subsequent mass spectrometry analysis. 使用多孔石墨化碳柱对低聚糖、糖醇和糖肽进行高效液相色谱分析，随后进行质谱分析。

Journal of chromatography Pub Date : 1993-09-03 DOI: 10.1016/0021-9673(93)83344-r

M J Davies, K D Smith, R A Carruthers, W Chai, A M Lawson, E F Hounsell

{"title":"Use of a porous graphitised carbon column for the high-performance liquid chromatography of oligosaccharides, alditols and glycopeptides with subsequent mass spectrometry analysis.","authors":"M J Davies, K D Smith, R A Carruthers, W Chai, A M Lawson, E F Hounsell","doi":"10.1016/0021-9673(93)83344-r","DOIUrl":"https://doi.org/10.1016/0021-9673(93)83344-r","url":null,"abstract":"HPLC using a porous graphitised carbon (PGC) column eluted in acetonitrile-aqueous trifluoroacetic acid has been shown to give complementary chromatography to reversed-phase (ODS) HPLC for separation of peptides and glycopeptides. The PGC column can also be used for separation of oligosaccharides and oligosaccharide alditols released from protein by enzymes (N-linked chains) or base-borohydride degradation (O-linked chains). The advantages are that peptides, glycopeptides, reducing oligosaccharides, sialylated oligosaccharides and oligosaccharide alditols can be chromatographed under the same conditions. The samples can be readily recovered by evaporation for sensitive liquid secondary ion mass spectrometric (LSI-MS) analysis and there is no contamination or deterioration of chromatography from column leakage. LSI-MS analysis revealed that complete peak separation of all of the possible oligosaccharide components of the standard glycoproteins fetuin and bovine submaxillary mucin was not achieved. However, PGC remains as a useful adjunct to other HPLC profiling and separation techniques in particular where subsequent MS analysis is desired.","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"646 2","pages":"317-26"},"PeriodicalIF":0.0,"publicationDate":"1993-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0021-9673(93)83344-r","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19392102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 85

The analysis of multiple phosphoseryl-containing casein peptides using capillary zone electrophoresis. 毛细管区带电泳分析多种含磷酸丝氨酸酪蛋白肽。

Journal of chromatography Pub Date : 1993-09-03 DOI: 10.1016/0021-9673(93)83352-s

N Adamson, P F Riley, E C Reynolds

{"title":"The analysis of multiple phosphoseryl-containing casein peptides using capillary zone electrophoresis.","authors":"N Adamson, P F Riley, E C Reynolds","doi":"10.1016/0021-9673(93)83352-s","DOIUrl":"https://doi.org/10.1016/0021-9673(93)83352-s","url":null,"abstract":"Multiple phosphoseryl-containing sequences of peptides and proteins stabilize amorphous calcium phosphate at neutral and alkaline pH and have been implicated in the nucleation/regulation of biomineralization. In an approach to analyze these peptides using capillary zone electrophoresis (CZE) we have attempted to relate the absolute electrophoretic mobility of various casein phosphopeptides to their physicochemical properties. Multiple phosphoseryl-containing peptides were selectively precipitated from enzymic digests of sodium caseinate and further purified using RP-HPLC and anion-exchange fast protein liquid chromatography. Purified fractions were then analyzed by CZE. Absolute electrophoretic mobilities of 13 peptides were determined by measurement of migration times relative to that of a neutral marker, mesityl oxide. A linear relationship (r2 = 0.993) was obtained between absolute electrophoretic mobility and q/M(r)2/3 where q is the net negative charge of the peptide calculated using relevant pKa values and M(r) is the molecular mass. M(r)2/3 is a measure of the surface area of a sphere that has a volume proportional to the M(r) of the peptide and relates to the frictional drag exerted on the peptide during electrophoretic migration. As absolute electrophoretic mobility is influenced by charge and size CZE can be used to monitor peptide phosphorylation, dephosphorylation, deamidation and truncation. This technique therefore would be suitable for quantitative analysis of peptide substrates in kinase and phosphatase studies. In conclusion CZE is a rapid and efficient technique for the resolution of multiple phosphoseryl-containing peptides from enzymic digests of casein.","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"646 2","pages":"391-6"},"PeriodicalIF":0.0,"publicationDate":"1993-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0021-9673(93)83352-s","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19393248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

High-performance liquid chromatography of amino acids, peptides and proteins. CXXXI. O-phosphoserine as a new chelating ligand for use with hard Lewis metal ions in the immobilized-metal affinity chromatography of proteins. 氨基酸、多肽和蛋白质的高效液相色谱。CXXXI。o -磷酸丝氨酸作为一种新的螯合配体与硬路易斯金属离子用于蛋白质的固定金属亲和层析。

Journal of chromatography Pub Date : 1993-08-27 DOI: 10.1016/s0021-9673(99)87012-5

M Zachariou, I Traverso, M T Hearn

{"title":"High-performance liquid chromatography of amino acids, peptides and proteins. CXXXI. O-phosphoserine as a new chelating ligand for use with hard Lewis metal ions in the immobilized-metal affinity chromatography of proteins.","authors":"M Zachariou, I Traverso, M T Hearn","doi":"10.1016/s0021-9673(99)87012-5","DOIUrl":"https://doi.org/10.1016/s0021-9673(99)87012-5","url":null,"abstract":"Conditions for the immobilization of O-phosphoserine (OPS) to epoxy-activated Sepharose CL-4B are described. The binding behaviour of OPS and iminodiacetic acid (IDA) immobilized onto Sepharose CL-4B, toward the hard Lewis metal ions Al3+, Fe3+, Ca2+ and Yb3+, and Cu2+ ion as a borderline metal ion control, over the pH range pH 4.0 to pH 8.0, was examined. Immobilized OPS shows a stronger affinity for Fe3+ and Al3+ ions but a lower affinity for Cu2+ and Yb3+ ions, compared to immobilized iminodiacetic acid (IDA), over the equilibrating range examined. Immobilized OPS-Mn+ was screened for protein binding using as model proteins tuna heart cytochrome c (THCC), horse myoglobin (HMYO) and hen egg while lysozyme (HEWL) over the pH range 5.5 to 8.0. Immobilized OPS-Fe3+ bound THCC under all the examined equilibrating conditions, bound HMYO between pH 5.5 and pH 7.0 and did not bind HEWL under any condition examined. Immobilized OPS thus presents an additional mode of metal ion and protein selectivity in immobilized-metal affinity chromatography.","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"646 1","pages":"107-20"},"PeriodicalIF":0.0,"publicationDate":"1993-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/s0021-9673(99)87012-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19394026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

Kinetics of recombinant human brain-derived neurotropic factor unfolding under reversed-phase liquid chromatography conditions. 反相液相色谱条件下重组人脑源性神经营养因子展开动力学。

Journal of chromatography Pub Date : 1993-08-27 DOI: 10.1016/s0021-9673(99)87010-1

K Benedek

引用次数: 17

Separation of membrane-embedded tryptic peptides of Na,K-ATPase by size-exclusion chromatography. Na, k - atp酶膜包埋色氨酸的排粒径色谱分离。

Journal of chromatography Pub Date : 1993-08-27 DOI: 10.1016/s0021-9673(99)87016-2

D M Tal, R Goldshleger, S J Karlish

{"title":"Separation of membrane-embedded tryptic peptides of Na,K-ATPase by size-exclusion chromatography.","authors":"D M Tal, R Goldshleger, S J Karlish","doi":"10.1016/s0021-9673(99)87016-2","DOIUrl":"https://doi.org/10.1016/s0021-9673(99)87016-2","url":null,"abstract":"Several attempts to separate hydrophobic tryptic and cyanogen bromide-digested short peptides from Na,K-ATPase, using HPLC and different acid-organic solvent gradients, failed because of the insolubility of the peptides in the initial or final solvents of the gradients used for elution. Therefore, we opted to use a detergent-containing mobile phase. For sodium dodecyl sulphate-solubilized tryptic peptides of M(r) 7 x 10(3)-100 x 10(3), elution on a TSK-G3000SW size-exclusion column successfully separates families of peptides with a resolution of M(r) 5 x 10(3)-10 x 10(3). Peptides in these size ranges can then be resolved completely by tricine-sodium dodecyl sulphate gel electrophoresis, and identified by microsequencing after transfer to polyvinylidene difluoride paper. For separation of smaller peptides a Biosep-SEC-S2000 column, eluted at slow flow-rates, was evaluated. Use of ammonium chloride buffer allows sensitive detection at 214 nm. The separated fractions are resolved and identified on 16.5% tricine gels. Reasonable resolution has been obtained with defined cyanogen bromide fragments of myoglobin. Resolution of small tryptic and cyanogen bromide fragments of Na,K-ATPase is less successful, but the experiments suggest ways of improving the resolution of peptides in the range M(r) 2 x 10(3)-10 x 10(3).","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"646 1","pages":"153-7"},"PeriodicalIF":0.0,"publicationDate":"1993-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/s0021-9673(99)87016-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19394029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mapping of the antibody- and receptor-binding domains of granulocyte colony-stimulating factor using an optical biosensor. Comparison with enzyme-linked immunosorbent assay competition studies. 利用光学生物传感器定位粒细胞集落刺激因子的抗体和受体结合域。与酶联免疫吸附法的比较研究。

Journal of chromatography Pub Date : 1993-08-27 DOI: 10.1016/s0021-9673(99)87017-4

E Nice, J Layton, L Fabri, U Hellman, A Engstrom, B Persson, A W Burgess

{"title":"Mapping of the antibody- and receptor-binding domains of granulocyte colony-stimulating factor using an optical biosensor. Comparison with enzyme-linked immunosorbent assay competition studies.","authors":"E Nice, J Layton, L Fabri, U Hellman, A Engstrom, B Persson, A W Burgess","doi":"10.1016/s0021-9673(99)87017-4","DOIUrl":"https://doi.org/10.1016/s0021-9673(99)87017-4","url":null,"abstract":"An automated optical biosensor instrument for measuring molecular interactions (Pharmacia BIAcore) has been used to characterise the epitopes recognised by 15 monoclonal antibodies raised against recombinant human granulocyte colony-stimulating factor (G-CSF). The BIAcore combines an autosampler and integrated microfluidic cartridge for the introduction and transportation of samples to the sensor chip surface, with surface plasmon resonance to detect binding events. A rabbit anti-mouse Fc antibody, coupled to the sensor surface in situ using conventional protein chemistry techniques, was used to capture an anti-G-CSF monoclonal antibody. G-CSF was bound to this antibody by injection over the sensor surface. Multi-site binding experiments were then performed in which other anti-G-CSF monoclonal antibodies were injected sequentially over the surface, and their ability to bind to the G-CSF in a multimolecular complex monitored in real time. Results obtained using the biosensor have been compared with data obtained by cross competition studies using biotinylated antibodies or antibody binding studies using chemically or enzymatically derived G-CSF peptide fragments or synthetic peptides. The results of these studies are in excellent agreement with the data from the BIAcore, although modification of the antibody or G-CSF occasionally altered the epitope affinity.","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"646 1","pages":"159-68"},"PeriodicalIF":0.0,"publicationDate":"1993-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/s0021-9673(99)87017-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18695106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34