Journal of cyclic nucleotide research最新文献_第9页

Observations on the level of cyclic nucleotides in three population of human lymphocytes in culture. 三种培养的人淋巴细胞中环核苷酸水平的观察。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

U Carpentieri, T M Monahan, L P Gustavson

引用次数: 0

Preparation of GTP sensitive adenylate cyclase from luteinized rat ovaries. 黄体化大鼠卵巢制备GTP敏感腺苷酸环化酶。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

P J Mcllroy, R J Ryan

引用次数: 0

(-)-[125I]-iodopindolol, a new highly selective radioiodinated beta-adrenergic receptor antagonist: measurement of beta-receptors on intact rat astrocytoma cells. (-)-[125I]-碘opindolol，一种新的高选择性放射性碘化β -肾上腺素能受体拮抗剂:完整大鼠星形细胞瘤细胞β -受体的测定

Journal of cyclic nucleotide research Pub Date : 1980-01-01

K Barovsky, G Brooker

{"title":"(-)-[125I]-iodopindolol, a new highly selective radioiodinated beta-adrenergic receptor antagonist: measurement of beta-receptors on intact rat astrocytoma cells.","authors":"K Barovsky, G Brooker","doi":"","DOIUrl":"","url":null,"abstract":"(-)-Pindolol, one of the most potent beta-adrenergic receptor antagonists, was radioiodinated using chloramine-T oxidation of carrier-free Na 125I and separated from unreacted pindolol to yield 2200 Ci/mmole (-)-[125I]-iodopindolol ((-)-[125I]-IPin). Mass and ultraviolet spectra confirmed that the iodination occurred on the indole ring, presumably at the 3 position. The binding of radiolabeled (-)-[125I]-IPin to beta-adrenergic receptors has been studied using intact C6 rat astrocytoma cells (2B subclone) grown in monolayer cultures. Binding of (-)-[125I]-IPin was saturable with time and concentration. Using 13 pM (-)-[125I]-IPin, binding equilibrium was reached in 90 min at 21-22 degrees C. The reverse rate constant was 0.026 min-1 at 21 degrees C. Specific binding (expressed as 1 microM (-)-propranolol displaceable counts) of (-)-[125I]-IPin was 95% of total binding. Scatchard analysis of (-)-[125I]-IPin binding revealed approximately 4300 receptors/cell and a dissociation constant of 30 pM. This was in excellent agreement with the kinetically determined dissociation constant of 35 pM. Displacement by propranolol and isoproterenol showed that (-)-[125I]-IPin binding sites were pharmacologically and stereospecifically selective. These results indicate that (-)-[125I]-IPin, a pure (-)-stereoisomer, high specific activity radioligand, selectively binds to beta-adrenergic receptors in whole cells with a high percentage of specific binding and should therefore be useful in the study and measurement of cellular beta-adrenergic receptors.","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"6 4","pages":"297-307"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17177053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alcohol potentiation of isoproterenol-stimulated cyclic AMP accumulation in rat parotid. 异丙肾上腺素刺激大鼠腮腺循环AMP积累的酒精增强作用。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

J F Harper, G Brooker

引用次数: 0

Interaction between alpha and beta adrenergic receptors and cholinergic receptors in isolated perfused rat heart: effects on cAMP-protein kinase and phosphorylase. 离体灌注大鼠心脏α和β肾上腺素能受体和胆碱能受体的相互作用:对camp蛋白激酶和磷酸化酶的影响。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

C G Ingebretsen

{"title":"Interaction between alpha and beta adrenergic receptors and cholinergic receptors in isolated perfused rat heart: effects on cAMP-protein kinase and phosphorylase.","authors":"C G Ingebretsen","doi":"","DOIUrl":"","url":null,"abstract":"The ability of acetylcholine to antagonize catecholamine-induced activation of myocardial cyclic AMP dependent protein kinase and glycogen phosphorylase activity was assessed using isolated perfused rat hearts. Perfused hearts were treated with either saline, epinephrine, epinephrine plus phentolamine or isoproterenol. After 1 minute of infusion of the indicated drug a second infusion containing acetylcholine was started. After an additional minute hearts were frozen and analyzed for cyclic nucleotide content and enzyme activity. In the presence of the alpha receptor blocking agent, phentolamine, epinephrine is a more effective activator of protein kinase than in its absence. Under these conditions the antagonistic action of acetylcholine on protein kinase activation is more pronounced. In the presence of epinephrine plus phentolamine or in the presence of isoproterenol the antagonistic action of acetylcholine on phosphorylase activity can be accounted for by a reduction in cyclic AMP-protein kinase. This same action of acetylcholine on epinephrine-stimulated phosphorylase in the aabsence of phentolamine, however, cannot be totally accounted for by a reduction in cyclic AMP content or in protein kinase activity.","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"6 2","pages":"121-32"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17315121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Studies of functional domains of the regulatory subunit from cAMP-dependent protein kinase isozyme I. camp依赖性蛋白激酶同工酶I调控亚基功能域的研究。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

S R Rannels, J D Corbin

{"title":"Studies of functional domains of the regulatory subunit from cAMP-dependent protein kinase isozyme I.","authors":"S R Rannels, J D Corbin","doi":"","DOIUrl":"","url":null,"abstract":"Homogenous regulatory subunit from rabbit skeletal muscle cAMP-dependent protein kinase (isozyme I) was partially hydrolyzed with low (1 g/1300 g) or high (1 g/6 g) concentrations of trypsin. After treatment with low trypsin two main peptides (Mr = 35,000 and 12,000) were produced. The cAMP-binding activity (2 mol cAMP/mol of subunit monomer) was recovered in the monomeric Mr = 35,000 peptide. The ability of either fragment to inhibit catalytic subunit activity was lost. Treatment of the regulatory subunit with a high concentration of trypsin yielded three main fragments (Mr = 32,000, 16,000, and 6,000) which could be resolved by Sephadex G-75 and purified further on DEAE-cellulose columns. One of the peptides (Mr = 32,000) bound 2 mol cAMP/mol fragment. The Mr = 16,000 fragment was very labile and bound cAMP with an undetermined stoichiometry. Cyclic AMP dissociation curves for the native regulatory subunit and its Mr = 32,000 component were similar and suggested the presence of two nonidentical binding sites in each monomer. Using the same procedure, the Mr = 16,000 fragment or homogenous cGMP-dependent protein kinase appeared to contain a single type of binding site. Purified Mr = 32,000 fragment was readily converted to the Mr = 16,000 fragment using high trypsin as assessed by protein bands on SDS-disc gels or by following transfer of radioactivity from Mr = 32,000 peptide covalently labeled with 8-N3-[32P] cAMP to radiolabeled Mr = 16,000 fragment. The smallest regulatory subunit fragment (Mr = 6,000) did not bind cAMP, but was dimeric and could be part of the dimerization domain in the native protein. A model is presented to explain the possible structural-functional relationships of the regulatory subunit.","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"6 3","pages":"201-15"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17317418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Export of cyclic AMP by mammalian reticulocytes. 哺乳动物网状细胞输出环状AMP。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

L L Brunton, J E Buss

引用次数: 0

Characterization of radiolabeled agonist binding to beta-adrenergic receptors in mammalian tissues. 哺乳动物组织中与β -肾上腺素能受体结合的放射性标记激动剂的特性。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

K A Heidenreich, G A Weiland, P B Molinoff

{"title":"Characterization of radiolabeled agonist binding to beta-adrenergic receptors in mammalian tissues.","authors":"K A Heidenreich, G A Weiland, P B Molinoff","doi":"","DOIUrl":"","url":null,"abstract":"Recent evidence suggests that the molecular interactions of agonists with beta-adrenergic receptors differ from those of antagonists. Most of this evidence has come from studies of agonist inhibition of radiolabeled antagonist binding. We have examined agonist binding directly in rat lung membranes using radiolabeled hydroxybenzylisoproterenol (3H-HBI). Specific binding of 3H-HBI was stereoselective and was inhibited by catecholamines with a potency order characteristic of beta 2-adrenergic receptors. Gpp(NH)p increased the rates of association and dissociation of 3H-HBI from the receptor. In the absence of Gpp(NH)p, Scatchard plots were curvilinear suggesting a complex interaction of the agonist with the receptor. The total number of 3H-HBI binding sites was similar to that of 125I-IHYP binding sites. In the presence of increasing concentrations of Gpp(NH)p, the affinity of 3H-HBI was decreased and Scatchard plots became linear. Sodium chloride mimicked the effect of Gpp(NH)p in lowering the affinity of the receptor for 3H-HBI. Magnesium chloride had the opposite effect in that it promoted high affinity binding. The effect of sodium chloride was largely overcome by the presence of magnesium chloride.","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"6 3","pages":"217-30"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17175502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of immunofluorescent cyclic GMP-positive fibres in the central nervous system. 中枢神经系统免疫荧光环gmp阳性纤维的表征。

Journal of cyclic nucleotide research Pub Date : 1979-12-01

R Cumming, G Arbuthnott, A Steiner

引用次数: 0

Prostacyclin increases cAMP in coronary arteries. 前列环素增加冠状动脉cAMP。

Journal of cyclic nucleotide research Pub Date : 1979-12-01

W R Kukovetz, S Holzmann, A Wurm, G Pöch

引用次数: 0