Journal of cyclic nucleotide research最新文献_第8页

Methyl 5'-(6-aminopurin-9-yl)-5'-deoxy-beta-D-ribofuranoside 2',3'-cyclic monophosphate: a novel, biologically active, structural analogue of cyclic AMP. 甲基5'-(6-氨基嘌呤-9-基)-5'-脱氧- β -d -核呋喃苷2'，3'-环单磷酸:一种新颖的，具有生物活性的环AMP结构类似物。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

V Nair, R J Wiechert, D E Vietti, D Singh, R H Stevens

引用次数: 0

Regulation of Ca2+ and cyclic AMP during the first meiotic division in amphibian oocytes by progesterone. 黄体酮对两栖动物卵母细胞第一次减数分裂过程中Ca2+和环AMP的调控。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

A B Kostellow, D Ziegler, G A Morrill

引用次数: 0

Inhibition of cholera toxin activation of the adenylate cyclase system in intact HeLa cells. 霍乱毒素对完整HeLa细胞腺苷酸环化酶系统激活的抑制作用。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

M C Lin, M Taniuchi

引用次数: 0

Alpha adrenergic receptor mediated formation of cyclic AMP in rat spinal cord. α肾上腺素能受体介导大鼠脊髓环AMP的形成。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

D J Jones, L F McKenna

{"title":"Alpha adrenergic receptor mediated formation of cyclic AMP in rat spinal cord.","authors":"D J Jones, L F McKenna","doi":"","DOIUrl":"","url":null,"abstract":"Methoxamine and phenylephrine (PE), postsynaptic alpha adrenergic agonists stimulated the accumulation of cyclic AMP in spinal cord tissue slices. Naphazoline, oxymetazoline and clonidine, previously shown to have greater efficacy at presynaptic alpha receptors did not alter accumulation and, in fact, blocked the PE response. The PE-stimulation was completely inhibited by postsynaptic alpha antagonists, incompletely by agents which bl ock presynaptic alpha receptors, and slightly by the beta blocker propranolol. Pe-stimulated accumulation was potentiated by phosphodiesterase inhibition (RO 20-1724). In contrast to previous reports on the requirement of the copresence of adenosine for alpha receptor stimulated accumulation of cyclic AMP in neuronal tissue, the PE-stimulation in spinal cord slices was unchanged by adenosine receptor blockade (theophylline), hydrolysis of endogenous adenosine (adenosine deaminase), inhibition of adenosine deaminase (EHNA) or blockade of adenosine uptake (dipyridamole). Added adenosine increased basal accumulation and produced a marked potentiation of the PE response. From this data it is evident that, in spinal cord tissue slices, there occurs a postsynaptic alpha adrenergic receptor linked to cyclic AMP accumulation which does not require the presence of other neurohumoral agents for activation.","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"6 2","pages":"133-41"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17171934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The turnover of cyclic AMP in cultured fibroblasts. 培养成纤维细胞中环AMP的周转。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

R Barber, R W Butcher

引用次数: 0

Temperature effects on cyclic AMP accumulation in cultured fibroblasts. 温度对培养成纤维细胞循环AMP积累的影响。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

R Barber, K P Ray, R W Butcher

引用次数: 0

Phosphatidic acid inhibition of PGE1-stimulated cAMP accumulation in WI-38 fibroblasts: similarities with carbachol inhibition. 磷脂酸抑制pge1刺激WI-38成纤维细胞cAMP积累:与碳醇抑制相似。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

R B Clark, D M Salmon, T W Honeyman

{"title":"Phosphatidic acid inhibition of PGE1-stimulated cAMP accumulation in WI-38 fibroblasts: similarities with carbachol inhibition.","authors":"R B Clark, D M Salmon, T W Honeyman","doi":"","DOIUrl":"","url":null,"abstract":"To test the hypothesis that phosphatidic acid (PhA) is involved in the carbachol inhibition of hormone stimulated accumulation of cAMP we observed the effects of PhA on PGE1-stimulation of cAMP in WI-38 fibroblasts. PhA inhibited PGE1-stimulated cAMP accumulation of WI-38 fibroblasts; maximum inhibition (approximately 50-80%) occurred at a PhA concentration of 1.0 microM and significant inhibition was observed with a concentration of 0.1 microM. The full effects of PhA were evident within 15 sec after the co-addition of PGE1 and PhA. Addition of PhA to cells which had been pre-stimulated with PGE1 resulted in the rapid decay of cAMP levels to a new steady state level with a t 1/2 of approximately 65 sec. The inhibition produced by PhA did not appear to be simply attributable to a depolarization or increased intracellular Ca2+, since addition of either KCl or the Ca2+ ionophore A23187 did not lower PGE1-stimulated cAMP accumulation. When intact cells were pretreated with PhA then lysed and adenylate cyclase immediately assayed, no detectable changes in broken cell adenylate cyclase activities were observed. Also, PhA added directly to adenylate cyclase assays at concentrations as high as 100 microM produced no detectable inhibition of the membrane fraction adenylate cyclase activities. Nonetheless, our results suggest that adenylate cyclase activity in intact cells may be directly affected by physiological levels of PhA . Further, the similarities of carbachol [Butcher, R. W., Journal of Cyclic Nucleotide Research, 4:411 (1978)] and PhA inhibition support the hypothesis that carbachol (acetylcholine) exerts its effect on adenylate cyclase through alterations of the plasma membrane phospholipid composition.","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"6 1","pages":"37-49"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17310069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Residual cyclic nucleotide associated with tissues after exposure to aqueous buffer analogous to that used in immunocytochemistry. 暴露于类似于免疫细胞化学中使用的水缓冲液后与组织相关的残余环核苷酸。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

R A Ortez

{"title":"Residual cyclic nucleotide associated with tissues after exposure to aqueous buffer analogous to that used in immunocytochemistry.","authors":"R A Ortez","doi":"","DOIUrl":"","url":null,"abstract":"A question concerning cyclic nucleotide immunocytochemical localization has been how much nucleotide remains associated with the tissue section. To answer that question cryostat sections of goldfish eye and mouse spleen, liver and lung were mounted on microscope slides and air dried. Following fixation by a variety of procedures employing heat, paraformaldehyde, glutaraldehyde, acetone, or ethanol, the sections were exposed to buffer (20 microM NaHPO4, 154 microM NaCl, pH 6.1) for 4 hours. The tissues were then scraped from the slides into 0.4N Perchloric Acid and cAMP and cGMP extracted and measured. The results show that fractions of both nucleotides are retained during buffer exposure. However, the amount retained varied with the: i) neucleotide, ii) fixation procedure, and iii) tissue type. Cyclic GMP retention was consistently higher (20-70%) than cAMP (6-30%). Glutaraldehyde was consistently more efficient in fixing cAMP, while cGMP retention was more variable with different fixation procedures. Tissue variability is seen in the example that spleen and liver retained more cGmp (71.4 and 70.6% respectively) than lung and eye (22.8 and 37.7% respectively). Maximum nucleotide loss occured during the first 5-30 minutes of buffer exposure with no additional loss accuring after another 20 hours. Collectively, these results demonstrate that cyclic nucleotides are retained during immunocytochemical staining procedures but that the degree of retention is dependent on several variables.","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"6 2","pages":"93-104"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17315123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A rapid direct assay of 3'-5' cyclic nucleotide phosphodiesterase activity using chromatography on immobilized acriflavin. 固定化吖啶黄素上3′-5′环核苷酸磷酸二酯酶活性的快速直接测定。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

C Rochette-Egly, J M Egly

引用次数: 0

Stable cholinergic-muscarinic and alpha-adrenergic inhibition of rat parotid adenylate cyclase. 大鼠腮腺腺苷酸环化酶稳定的胆碱能-毒蕈碱和α -肾上腺素能抑制作用。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

Y Oron, S Creacy, J Kellogg, J Larner

{"title":"Stable cholinergic-muscarinic and alpha-adrenergic inhibition of rat parotid adenylate cyclase.","authors":"Y Oron, S Creacy, J Kellogg, J Larner","doi":"","DOIUrl":"","url":null,"abstract":"(-) Isoproterenol-stimulated adenylate cyclase activity of washed at parotid membrane preparations from gland slices previously treated with the alpha-adrenergic agonist, methoxamine, was inhibited approximately 30%. The action of methoxamine required exogenous Ca2+ and was blocked by the alpha-adrenergic blocking agent, phentolamine. Incubation of gland slices with carbamylcholine resulted in a dose-dependent inhibition (50%) of (-) isoproterenol-stimulated adenylate cyclase activity in washed membranes. The action of carbamylcholine was independent of exogenous Ca2+ and was blocked by preincubation with atropine. Cholinergic inhibition of parotid adenylate cyclase was compared to the cholinergic inhibition of adenylate cyclase in dog heart homogenates. While carbamylcholine caused a limited (20-30%) inhibition of basal and (-) isoproterenol-stimulated activities when added to dog heart homogenates, it failed to produce any effect in parotid homogenates prepared in an identical manner. Cholinergic inhibition of parotid adenylate cyclase activity was stable and persisted in washed particulate fractions while the inhibition in dog heart homogenates was reversible by washing. Cholinergic inhibition of adenylate cyclase was markedly dependent on the presence of GTP and was abolished when Mn2+ was subsituted for Mg2+ in both systems. Guanyl-5'-yl imidodiphosphate had little effect on the inhibition in parotid preparations but abolished the inhibition in heart homogenates. It is concluded that, in contrast to the cholinergic action in dog heart homogenates, the exposure of parotid slices to carbamylcholine results in a stable lesion in the adenylate cyclase activity.","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"6 2","pages":"105-20"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18420907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0