Journal of cyclic nucleotide research最新文献_第6页

A simple fluorometric method for cAMP: application to studies of brain adenylate cyclase activity. 一种简单的cAMP荧光测定方法:在脑腺苷酸环化酶活性研究中的应用。

Journal of cyclic nucleotide research Pub Date : 1981-01-01

W Wojcik, M Olianas, M Parenti, S Gentleman, N H Neff

引用次数: 0

Theories and predictions of models describing sequential interactions between the receptor, the GTP regulatory unit, and the catalytic unit of hormone dependent adenylate cyclases. 描述受体、GTP调控单元和激素依赖性腺苷酸环化酶催化单元之间顺序相互作用的模型的理论和预测。

Journal of cyclic nucleotide research Pub Date : 1981-01-01

A M Tolkovsky, A Levitzki

引用次数: 0

Distribution of cyclic nucleotides in layers of the cerebellum after decapitation. 斩首后小脑各层环核苷酸的分布。

Journal of cyclic nucleotide research Pub Date : 1981-01-01

W D Lust, G K Feussner, J V Passonneau, D W McCandless

引用次数: 0

Comparison of cAMP-dependent protein kinase in normal and Rous sarcoma virus transformed chick embryo fibroblasts. camp依赖性蛋白激酶在正常和劳斯肉瘤病毒转化的鸡胚成纤维细胞中的表达比较。

Journal of cyclic nucleotide research Pub Date : 1981-01-01

J E Kudlow, R K Watson, G N Gill

引用次数: 0

Cautions on the use of the heat stable inhibitor of protein kinase: studies with S49 lymphoma cells. 热稳定蛋白激酶抑制剂的使用注意事项:对S49淋巴瘤细胞的研究。

Journal of cyclic nucleotide research Pub Date : 1981-01-01

J R Kanter, L L Brunton

引用次数: 0

Membrane milieu is regarded as the native environment of the cyclic AMP degrading enzyme cluster. 膜环境被认为是环AMP降解酶簇的天然环境。

Journal of cyclic nucleotide research Pub Date : 1981-01-01

H Wombacher

{"title":"Membrane milieu is regarded as the native environment of the cyclic AMP degrading enzyme cluster.","authors":"H Wombacher","doi":"","DOIUrl":"","url":null,"abstract":"The phenomenon of kinetic advantage of nucleoside formation from cyclic AMP, via the intermediate 5'AMP has been observed in the microsomal fraction after subcellular fractionation of beef adrenal cortex tissue. It was explained by the existence of a multienzyme sequence previously evidenced [H. Wombacher, 1982, Arch. Biochem. Biophys. 201, 8-19]. In the present study a similar enzyme cluster was prepared from the soluble fraction of the cell homogenate after two steps of gel-chromatography. An elusive channeling of cyclic AMP degradation could be disclosed. The time course reaction of cyclic AMP degradation to the nucleosides, adenosine and inosine, via 5'AMP as an intermediate compared with the time course reaction of 5'AMP hydrolysis to the nucleosides, adenosine and inosine, under otherwise identical conditions showed that the nucleoside formation from cyclic AMP was faster after the lag phase of the reaction sequence. This kinetic advantage effect, however, was much less pronounced than to be seen in the membrane-bound multienzyme sequence. For an analysis of the influence of the environmental conditions on the activity of both enzyme cluster forms they were treated by chaotropic agents, detergents and ultrasonic power. Common to all results was: the activity of the membrane-bound enzyme cluster is highly stable in comparison with the soluble form. On basis of these and previous findings a hypothesis is suggested explaining the similarities between the membrane-bound enzyme cluster and the soluble form. Thus, the soluble enzyme cluster form is considered a partially preserved form of the membrane-bound form arisen from the cell homogenization process and/or vice versa the soluble form might present a pro-form of the membrane-bound enzyme cluster, and the most stable and active assembly has to be yet first membrane-triggered.","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"7 5","pages":"293-302"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17347142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of isoproterenol treatment of isolated perfused rat hearts on myofibrillar phosphorylation and ATPase activity. 异丙肾上腺素对离体灌注大鼠心肌纤维磷酸化和atp酶活性的影响。

Journal of cyclic nucleotide research Pub Date : 1981-01-01

F D Sistare, L Lichtenberg, R G Sugg, S A McFarland, C Villar-Palasi

引用次数: 0

[125I]iodopindolol: a new beta adrenergic receptor probe. [125I]碘匹多洛尔:一种新的β肾上腺素能受体探针。

Journal of cyclic nucleotide research Pub Date : 1981-01-01

E G Ezrailson, A J Garber, P J Munson, T L Swartz, L Birnbaumer, M L Entman

{"title":"[125I]iodopindolol: a new beta adrenergic receptor probe.","authors":"E G Ezrailson, A J Garber, P J Munson, T L Swartz, L Birnbaumer, M L Entman","doi":"","DOIUrl":"","url":null,"abstract":"When utilizing iodohydroxybenzylpindolol (IHYP) as an adrenergic receptor probe in muscle membrane systems, the data demonstrated an unacceptably high nonspecific binding component. Bearer et al. have reported that chloramine-T induced iodination of hydroxybenzylpindolol (HYP) results in the incorporation of iodine into the indole ring rather than into the phenolic moiety as noted previously by others. These results suggest that pindolol itself can also be iodinated. Therefore, the usefulness of carrier free 125I-labeled iodopindolol (IPIN) as an adrenergic receptor probe was investigated. Using between 0.01 nM and 0.1 nM [125I]IPIN in two different muscle membrane systems, we found the nonspecific binding component to be 10% or less of total binding. When [125I]IPIN was used with membranes prepared from rat skeletal muscle, we found it to interact with a single set of high affinity binding sites (KD = 0.13 +/- 0.01 nM) with the characteristics of beta adrenergic receptors and a density of 48.5 fmoles/mg protein. IPIN binding was also studied with purified dog cardiac sarcolemma. A single set of binding sites was detected having a KD of 1.64 +/- 0.5 nM; the density of these sites was 289 fmoles/mg membrane protein. [125I]IPIN may be a useful probe for the beta adrenergic receptor of tissues in which [125I]IHYP and other beta adrenergic receptor probes have a non-specific binding component which approaches that of the specific binding component.","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"7 1","pages":"13-26"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17327901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purified guanylate cyclase: characterization, iodination and preparation of monoclonal antibodies. 纯化鸟苷酸环化酶:鉴定、碘化和单克隆抗体的制备。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

J A Lewicki, H J Brandwein, S A Waldman, F Murad

引用次数: 0

Activation of human lymphocyte high affinity cyclic AMP phosphodiesterase by culture with 1-methyl-3-isobutylxanthine. 1-甲基-3-异丁基黄嘌呤培养活化人淋巴细胞高亲和力环AMP磷酸二酯酶。

Journal of cyclic nucleotide research Pub Date : 1980-01-01

W J Thompson, C P Ross, E M Hersh, P M Epstein, S J Strada

{"title":"Activation of human lymphocyte high affinity cyclic AMP phosphodiesterase by culture with 1-methyl-3-isobutylxanthine.","authors":"W J Thompson, C P Ross, E M Hersh, P M Epstein, S J Strada","doi":"","DOIUrl":"","url":null,"abstract":"When 1-methyl-3-isobutylxanthine (MIX), a potent inhibitor of human lymphocyte cyclic AMP phosphodiesterase in vitro, was added to intact lymphocyte cultures, MIX caused an activation of this enzyme. Activation by MIX which showed an EC50 = 0.2 mM, required 24 hr in culture, reached a maximum near 48 hr, and remained at maximum through 144 hr. The effect of MIX was to increase the maximum velocity of the 3.5-4S form but not the 5.5-6S form of the lymphocyte high affinity enzyme system. Activation required serum, occurred in calcium depleted media, and was unaffected by sodium azide, adenosine, cycliheximide, or actinomycin D. Further, the effect of MIX was not mimicked by cyclic AMP, cyclic GMP, or by a variety of cyclic nucleotide derivatives with the exception of N6-monobutyryl- and N6-2'0 dibutyryl cyclic AMP in the culture medium. No correlation was observed between the in vitro IC.50 value and the amount of intact cell activation with other phosphodiesterase inhibitors. Although MIX prevented the mitogenic response of lymphocytes to phytohemaglutinin (PHA), a synergistic effect to activate cyclic nucleotide phosphodiesterase was obtained when both agents were included in the culture medium. These data are discussed as an example of a novel modulation of a specific high affinity form of cyclic AMP phosphodiesterase by an apparent non-genetic regulatory mechanism.","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"6 1","pages":"25-36"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17221664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0