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Journal of cyclic nucleotide research最新文献

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Cyclic nucleotides and the control of epithelial cell proliferation: cyclic CMP may be a partial mediator of the response of the pigeon crop-sac to prolactin. 环核苷酸和上皮细胞增殖的控制:环CMP可能是鸽囊对催乳素反应的部分中介。
Journal of cyclic nucleotide research Pub Date : 1981-01-01
T R Anderson, G L Mayer, C S Nicoll
{"title":"Cyclic nucleotides and the control of epithelial cell proliferation: cyclic CMP may be a partial mediator of the response of the pigeon crop-sac to prolactin.","authors":"T R Anderson,&nbsp;G L Mayer,&nbsp;C S Nicoll","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The possible role of cyclic nucleotides as second messengers mediating hormone-induced cellular growth in vivo was investigated using the proliferative response of the pigeon crop-sac mucosal epithelium to prolactin (PRL) as a model system. Local injections of cAMP, cCMP, cGMP, cTMP or cUMP alone over the prolactin-responsive cells had no mitogenic effect. When injected along with a small dose of PRL, cAMP at doses above 1 micromole inhibited the response by 10%. While cGMP at a dose of 10 micromoles augmented the response by 47%. In fact, cCMP gave a log-linear dose-response relationship with significant augmentation of the response to PRL observed with doses as low as 0.01 micromole. Cyclic UMP, cTMP and various 5'-nucleotide monophosphates had no effect on the response to the hormone. When the dibutyryl analogs of the cyclic nucleotides were tested for their ability to potentiate the response to PRL, only dibutyryl cCMP was effective. These data suggest that cCMP may be a partial mediator of the proliferative action of PRL in this system, but it is probably not the sole second messenger for prolactin's action in these cells. Our results also indicate that failure of a cyclic nucleotide to mimic a hormone-induced response by itself does not prove that the compound is uninvolved in the hormones's action.</p>","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"7 4","pages":"225-33"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17340390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Forskolin: a unique diterpene activator of cyclic AMP-generating systems. 福斯克林:一种独特的二萜活化剂的循环amp生成系统。
Journal of cyclic nucleotide research Pub Date : 1981-01-01
K B Seamon, J W Daly
{"title":"Forskolin: a unique diterpene activator of cyclic AMP-generating systems.","authors":"K B Seamon,&nbsp;J W Daly","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Forskolin, a diterpene of the labdane family, activates adenylate cyclase in broken cell preparations as well as in intact tissues. This activation does not require the guanine nucleotide regulatory subunit of the enzyme and probably occurs via an interaction with the catalytic subunit of adenylate cyclase. Activation of adenylate cyclase by forskolin results in marked increases in levels of intracellular cyclic AMP in a variety of eukaryotic cells. Low concentrations of forskolin which alone elicit small increases in intracellular cyclic AMP greatly potentiate hormonal activation of adenylate cyclase in a number of intact cells. Forskolin elicits cellular responses which have been proposed to be dependent o cyclic AMP as a second messenger. Forskolin, thus provides an invaluable tool for the investigation of the role of cyclic AMP in physiological responses to hormones, both through it direct activation of adenylate cyclase and through its ability to potentiate hormonal activation of adenylate cyclase.</p>","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"7 4","pages":"201-24"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17340389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibition by ADP of prostaglandin induced accumulation of cyclic AMP in intact human platelets. ADP抑制前列腺素诱导的环AMP在完整人血小板中的蓄积。
Journal of cyclic nucleotide research Pub Date : 1981-01-01
D E Macfarlane, D C Mills
{"title":"Inhibition by ADP of prostaglandin induced accumulation of cyclic AMP in intact human platelets.","authors":"D E Macfarlane,&nbsp;D C Mills","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The influence of extracellular ADP on cyclic AMP accumulation within intact human platelets was studied. ADP inhibited the increase in cyclic AMP which occurs when platelets are exposed to prostaglandin E1 or I2. The degree of inhibition varied in the range 70-95% , and was half maximal at ADP concentrations of between 0.3 and 2 microM. Other naturally occurring diphosphates, i.e. GDP, IDP and UDP, were at least 100 fold less effective than ADP, and UDP at 1mM partially reversed the effect of ADP. The effect by ADP was completely reversed by ATP, but only attenuated to a minor degree of 10 mM EDTA. Increasing concentrations of ADP caused a progressive degree of inhibition of cyclic AMP accumulation, and the kinetics of this inhibition were compatible with a simple saturable process with no cooperativity. ADP added 10 seconds after prostaglandin E1 blocked cyclic AMP accumulation within 1-2 seconds, and addition of ATP after ADP and prostaglandin I2 relieved the inhibition due to ADP within 2-3 seconds. The action of ADP was blocked by sulphydryl reagents including N-substituted maleimides, cytochalasin A, NBD chloride and p-mercuribenzene sulphonate. The data were considered to be consistent with mediation of the ADP effect through a sulphydryl-bearing specific extracellular receptor coupled to the adenylate cyclase.</p>","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"7 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17328798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new principle for resistance to cholera: desensitization to cyclic AMP-mediated diarrhea induced by cholera toxin in the mouse intestine. 霍乱抗性的新原理:对霍乱毒素引起的小鼠肠道环amp介导的腹泻脱敏。
Journal of cyclic nucleotide research Pub Date : 1981-01-01
I Lönnroth, S Lange
{"title":"A new principle for resistance to cholera: desensitization to cyclic AMP-mediated diarrhea induced by cholera toxin in the mouse intestine.","authors":"I Lönnroth,&nbsp;S Lange","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The mechanisms behind the intestinal resistance to cholera toxin was studied in a mouse model. Repeated peroral treatments with cholera toxin (CT) led to a long-lasting inhibition of the toxin-induced activation of intestinal adenylate cyclase (AC). A corresponding inhibition of the intestinal fluid secretion induced not only by CT but also by prostaglandin E1 was observed. This unspecific desensitization was followed by a CT-specific inhibition of secretion and AC after 8 to 16 days. The desensitization to CT was totally reversed by a 4 hour-treatment with cycloheximide, an inhibitor of protein synthesis. Neither the secretory response to dibutyryl-cyclic AMP nor the activity of soluble phosphodiesterase differed between the CT-treated mice and the control group. Nor was the average turn-over rate of intestinal cells changed as judged from the mucosal incorporation of [3H]-thymidine. It is postulated that intestinal resistance to CT is mainly a function of AC-desensitization mediated by an inducible protein.</p>","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"7 4","pages":"247-57"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17340210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Guanine nucleotides modulate the affinity of antagonists at beta-adrenergic receptors. 鸟嘌呤核苷酸调节拮抗剂对β -肾上腺素能受体的亲和力。
Journal of cyclic nucleotide research Pub Date : 1981-01-01
B B Wolfe, T K Harden
{"title":"Guanine nucleotides modulate the affinity of antagonists at beta-adrenergic receptors.","authors":"B B Wolfe,&nbsp;T K Harden","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Investigation of the properties of the binding of the radiolabelled antagonists (125I)-iodohydroxybenzylpindolol, (125I)-iodopindolol, and (125I)-iodocyanopindolol to beta-adrenergic receptors of L6 myoblast membranes revealed that guanine nucleotides caused a 2 to 4.5 fold increase in the apparent affinity of these antagonists. No significant effects of GTP were observed on the density of binding sites determined with each radioligand. GTP, GDP, and GMPPNP were of similar high affinity in producing this effect, while GMP was much less potent, and ATP was without effect. Under similar assay conditions GTP reduced the apparent binding affinity of the agonist isoproterenol for the beta-adrenergic receptors of L6 cells. The results indicate that, contrary to previous observations, guanine nucleotides affect not only the interactions of agonists with beta-adrenergic receptors, but also the interaction of antagonists with these adenylate cyclase-linked receptors.</p>","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"7 5","pages":"303-12"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17191113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential effects of cholera toxin on guanine nucleotide regulation of beta-adrenergic agonist high affinity binding and adenylate cyclase activation in frog erythrocyte membranes. 霍乱毒素对鸟嘌呤核苷酸调节β -肾上腺素能激动剂高亲和力结合和腺苷酸环化酶在青蛙红细胞膜上活化的差异影响。
Journal of cyclic nucleotide research Pub Date : 1981-01-01
J M Stadel, R J Lefkowitz
{"title":"Differential effects of cholera toxin on guanine nucleotide regulation of beta-adrenergic agonist high affinity binding and adenylate cyclase activation in frog erythrocyte membranes.","authors":"J M Stadel,&nbsp;R J Lefkowitz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The guanine nucleotide regulatory protein(s) regulates both adenylate cyclase activity and the affinity of adenylate cyclase-coupled receptors for hormones or agonist drugs. Cholera toxin catalyzes the covalent modification of the nucleotide regulatory protein of adenylate cyclase systems. Incubation of frog erythrocyte membranes with cholera toxin and NAD+ did not substantially alter the dose dependency for guanine nucleotide activation of adenylate cyclase activity. In contrast, toxin treated membranes demonstrated a 10 fold increase in the concentrations of guanine nucleotide required for a half maximal effect in regulating beta-adrenergic receptor affinity for the agonist (+/-) [3H]hydroxybenzylisoproterenol. The data emphasize the bifunctional nature of the guanine nucleotide regulatory protein and suggest that distinct structural domains of the guanine nucleotide regulatory protein may mediate the distinct regulatory effects on adenylate cyclase and receptor affinity for agonists.</p>","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"7 6","pages":"363-74"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17192388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evidence for a D1 dopamine receptor in the salivary glands of Amblyomma americanum (L.). 美洲钝瘤(Amblyomma americanum)唾液腺D1多巴胺受体的证据。
Journal of cyclic nucleotide research Pub Date : 1981-01-01
S P Schmidt, R C Essenberg, J R Sauer
{"title":"Evidence for a D1 dopamine receptor in the salivary glands of Amblyomma americanum (L.).","authors":"S P Schmidt,&nbsp;R C Essenberg,&nbsp;J R Sauer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Adenylate cyclase from the salivary gland of the lone star tick, Amblyomma americanum, is stimulated by several derivatives of phenylethylamine; dopamine, noradrenaline, adrenaline and isoproterenol. Octopamine and iota-DOPA had no effect on basal adenylate cyclase activity. Dopamine had the highest potency and the lowest Ka, 0.4 micron; followed by adrenaline and noradrenaline, 23 micron; and isoproterenol, 0.15mM. When either noradrenaline or adrenaline was added to dopamine, each at its maximally effective concentration, no additional stimulation of gland cyclase activity was noted. Furthermore, adrenaline and noradrenaline together did not equal the potency of dopamine alone. The most potent inhibitors of gland cyclase activity were the dopamine receptor antagonists. The phenothiazine drugs (thioridazine, chlorpromazine, and fluphenazine) were more effective inhibitors of cyclase activity than the butyrophenone drug (haloperidol). The inhibition constants (Ki) for the phenothiazine drugs were: 60nM for thioridazine, 1.9 micron for chlorpromazine and 2.3 micron for fluphenazine. The inhibition of adenylate cyclase activity was found to be specific for the (+) enantiomer of butaclamol, a stereospecific dopamine receptor antagonist. Our findings suggest that the lone star tick salivary gland adenylate cyclase has a D1 type dopamine receptor.</p>","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"7 6","pages":"375-84"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18359201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A sensitive equilibrium binding assay for soluble beta-adrenergic receptors. 可溶性β -肾上腺素能受体的灵敏平衡结合试验。
Journal of cyclic nucleotide research Pub Date : 1981-01-01
K M Witkin, T K Harden
{"title":"A sensitive equilibrium binding assay for soluble beta-adrenergic receptors.","authors":"K M Witkin,&nbsp;T K Harden","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An equilibrium binding assay has been developed for digitonin-solubilized beta-adrenergic receptors using 125 I-pindolol (IPIN) as a radioligand. Up to 50% of the beta-adrenergic receptors from rat lung membranes could be solubilized using 1% digitonin. Following incubation of soluble fractions with IPIN at 25 degree, protein associated radioactivity was identified by column chromatography using Sephadex G-50. The solubilized receptors bound IPIN with properties similar but not identical to those of the membrane bound receptor. The Kd determined for IPIN binding to soluble receptors was 113 pM while the Kd for membrane associated receptors was 36 pM. The rate constant for association (k1) of IPIN was 0.15x10(9) M-1 for soluble receptors and 2.2x10(9) M-1 min-1 for lung membrane receptors. The rate constant for dissociation (k2) was 0.025 min-1 for soluble receptors and 0.048 min-1 for membrane receptors. Agonists and antagonist of beta-adrenergic receptors inhibited in a stereoselective manner the binding of IPIN to both soluble and membrane bound receptors. The affinities of individual drugs determined for soluble receptors were similar to those determined for membrane receptors. Not only could digitonin-solubilized receptors be identified in soluble preparations from rat lung, but also from rat cerebral cortex and liver, and from L6 muscle, C6 rat glioma, and 1321N1 astrocytoma cell membranes.</p>","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"7 4","pages":"235-46"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17340209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Discovery and recognition of calmodulin: a personal account. 钙调素的发现与识别:个人叙述。
Journal of cyclic nucleotide research Pub Date : 1981-01-01
W Y Cheung
{"title":"Discovery and recognition of calmodulin: a personal account.","authors":"W Y Cheung","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"7 2","pages":"71-84"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17340387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hypothesis: cyclic AMP turnover in S49 cells. 假设:在S49细胞中AMP循环转换。
Journal of cyclic nucleotide research Pub Date : 1981-01-01
R Barber, T J Goka
{"title":"Hypothesis: cyclic AMP turnover in S49 cells.","authors":"R Barber,&nbsp;T J Goka","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The fractional turnover constant (ke) of cAMP in S49 lymphoma cells stimulated by epinephrine was essentially identical to the decay constant for cAMP in these cells. This was in sharp distinction to the situation in the human diploid lung fibroblast WI-38, in which ke was much lower in hormone stimulated cells. In this study we report a new method for the determination of cAMP turnover. The method was based on the assumption that for tritium label to be incorporated into cAMP on treatment of cells with [3H]-adenine, the label must first pass through ATP. An equation relating the rate of change in cAMP specific radioactivity with cAMP and ATP specific radioactivities was thereby determined. The equation was expressed in a form that gave a linear graphical plot with the fractional turnover constant as the slope of the line.</p>","PeriodicalId":15497,"journal":{"name":"Journal of cyclic nucleotide research","volume":"7 6","pages":"353-61"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17348697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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