Journal of Clinical Toxicology最新文献_第10页

Attenuation of CCl4 Induced Oxidative Stress, Immunosuppressive, HepatorenalDamage by Fucoidan in Rats 褐藻糖聚糖对CCl4诱导的大鼠氧化应激、免疫抑制、肝肾损伤的抑制作用

Journal of Clinical Toxicology Pub Date : 2017-05-16 DOI: 10.4172/2161-0495.1000348

M. El-Boshy, Fatma Abdelhamidb, Engy Richab, Ahmad Ashshia, Mazen Gaitha, Naeem Qustya

{"title":"Attenuation of CCl4 Induced Oxidative Stress, Immunosuppressive, HepatorenalDamage by Fucoidan in Rats","authors":"M. El-Boshy, Fatma Abdelhamidb, Engy Richab, Ahmad Ashshia, Mazen Gaitha, Naeem Qustya","doi":"10.4172/2161-0495.1000348","DOIUrl":"https://doi.org/10.4172/2161-0495.1000348","url":null,"abstract":"The protective and therapeutic effects of fucoidan extract from Laminaria species against liver damage induced by CCl4 in rats was investigated by monitoring the serum level and hepatic m-RNA expression of TGFβ-1, liver and renal markers, as well as oxidative stress and antioxidant biomarker. Thirty six adult male albino rats were divided into 4 equal groups; one was used as a negative control while groups II, III, and IV administrated 0.1 mL/100 g body weight twice a week for 8 weeks with carbon tetrachloride (CCl4), fucoidan (400 mg/kgbw orally/day), and CCl4 plus fucoidan, respectively. Blood samples were collected at the end of experiment and sera were separated to evaluate serum levels and the hepatic m-RNA expression of transforming growth factor beta (TGFβ-1), tumor necrosis factor (TNF α), interferon gamma (IFN-γ.), interleukin (IL), Il-1β, IL-6 and IL-10, antioxidant markers, reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and lipid peroxidation malondialdehyde (MDA) as well as selective biochemical markers of liver and kidney functions were estimated. The results of this investigation revealed that treatment with fucoidan improved elevated expression of liver TGF β-1, Il-1β, IL-6, TNF α and serum level of malnoaldehyde (MDA), total bilirubin (T. Bil), induced by CCl4 at 8th week post treatment. In addition to enhancing the antioxidant enzyme activities, GSH, GPx, CAT and SOD. Also, liver trransaminase (ALT, AST), alkaline phosphatase (ALP), reduced in fucoidan and CCl4 treated group. These results show that crude fucoidan has potential immunomodulatory, antioxidant and hepatoprotective effects against the hepatic damage induced by CCl4.","PeriodicalId":15433,"journal":{"name":"Journal of Clinical Toxicology","volume":"118 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88010418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Cytotoxicity Studies of Functionalized Gold Nanoparticles Using Yeast CometAssay 利用酵母CometAssay研究功能化金纳米颗粒的细胞毒性

Journal of Clinical Toxicology Pub Date : 2017-05-15 DOI: 10.4172/2161-0495.1000347

S. Suvarna, Rajesha K. Nairy, C. SunilK, Y. Narayana

{"title":"Cytotoxicity Studies of Functionalized Gold Nanoparticles Using Yeast CometAssay","authors":"S. Suvarna, Rajesha K. Nairy, C. SunilK, Y. Narayana","doi":"10.4172/2161-0495.1000347","DOIUrl":"https://doi.org/10.4172/2161-0495.1000347","url":null,"abstract":"In the present study gold nanoparticles and glucose capped gold nanoparticles are synthesized by chemical route method and characterized using UV-SPR, FTIR and TEM analysis. Single cell gel electrophoresis (SCGE) assay was used to study DNA damage. Studies show that glucose capped gold nanoparticles are less toxic as compare to gold nanoparticles at DNA level. Somewhat larger gold nanoparticle used to monitor endocytosis in log-phase S. cervisiae spheroplasts at 10 to 30 μM was not reported to cause growth inhibition. It shows that glucose capped gold nanoparticles are nontoxic to yeast strain D7. DNA damage was observed by using standard method called Yeast comet assay, which provides a very sensitive method for detecting strand breaks and repair kinetics in single cells. Studies showed that 5 μM-30 μM having very less sign of DNA damage in case of Glucose capped gold nanoparticles and it also shows toxic effect for without glucose capped gold nanoparticles. OTM for different concentration as shown in the image and OTM with respect to different concentration shows the DNA damage, these studies also correlated with survival studies.","PeriodicalId":15433,"journal":{"name":"Journal of Clinical Toxicology","volume":"7 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80233346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Lead Poisoning Induced Severe Hemolytic Anemia, Basophilic Stippling,Mimicking Erythrocyte Pyrimidine 5'-nucleotidase Deficiency in BetaThalassemia Minor 铅中毒引起的严重溶血性贫血，嗜碱性点染，模仿轻微β -血友病红细胞嘧啶5′-核苷酸酶缺乏

Journal of Clinical Toxicology Pub Date : 2017-04-27 DOI: 10.4172/2161-0495.1000346

P. Warang, R. Colah, P. Kedar

{"title":"Lead Poisoning Induced Severe Hemolytic Anemia, Basophilic Stippling,Mimicking Erythrocyte Pyrimidine 5'-nucleotidase Deficiency in BetaThalassemia Minor","authors":"P. Warang, R. Colah, P. Kedar","doi":"10.4172/2161-0495.1000346","DOIUrl":"https://doi.org/10.4172/2161-0495.1000346","url":null,"abstract":"Lead is a highly toxic metal and a very strong poison. Lead poisoning usually occurs over a period of months or years. The poisoning can cause severe mental and physical impairment. Young children are most vulnerable to lead poisoning [1-2]. Lead poisoning is accompanied by an acquired deficiency of erythrocyte pyrimidine 5'nucleotidase (P5’N). Genetically determined deficiency of P5’N enzyme was associated with chronic hemolysis, marked basophilic stippling of erythrocytes on peripheral blood smear and accumulations of intra-erythrocyte pyrimidine-containing nucleotides [3-4]. Pyrimidine-containing nucleotides are almost absent in the normal erythrocytes but it was reported that in lead poisoning, 12% of erythrocyte showed accumulation of pyrimidine nucleotides in the blood of a patient and P5’N activity was suppressed to 50% that in normal erythrocytes in lead poisoning [2]. In most of βthalassemia carriers and other hemoglobin variant (Hb-E) showed marginally reduced Purine/Pyrimidine nucleotide ratios but normal P5’N-1 activity [5]. This report describes the clinical severity of leadinduced hemolytic anemia in two Indian patients with basophilic stippling associated with intra-erythrocyte accumulations of pyrimidine-containing nucleotides which mimicking hereditary P5’N deficiency.","PeriodicalId":15433,"journal":{"name":"Journal of Clinical Toxicology","volume":"13 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2017-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76669276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Ventricular Fibrillation Caused by Mianserin Poisoning 勉色林中毒致心室颤动

Journal of Clinical Toxicology Pub Date : 2017-04-25 DOI: 10.4172/2161-0495.1000345

T. Kitamoto, K. Hayakawa, Fukuki Saito, Y. Nakamori

引用次数: 0

Atherogenic and Haematologic Indices of Paracetamol- Overdosed Albino RatsTreated With Aqueous Leaf Extracts of Euphorbia Heterophylla and JatrophaCurcas 对乙酰氨基酚过量白化病大鼠与大麻风叶提取物的动脉粥样硬化和血液学指标

Journal of Clinical Toxicology Pub Date : 2017-03-31 DOI: 10.4172/2161-0495.1000344

Chidi Uzoma Igwe, L. Nwaogu, Emmanuel Uche Olunkwa, Martin Otaba, V. Onwuliri

{"title":"Atherogenic and Haematologic Indices of Paracetamol- Overdosed Albino RatsTreated With Aqueous Leaf Extracts of Euphorbia Heterophylla and JatrophaCurcas","authors":"Chidi Uzoma Igwe, L. Nwaogu, Emmanuel Uche Olunkwa, Martin Otaba, V. Onwuliri","doi":"10.4172/2161-0495.1000344","DOIUrl":"https://doi.org/10.4172/2161-0495.1000344","url":null,"abstract":"Objective: The protective effect of aqueous leaf extracts of Euphorbia Heterophylla and Jatropha Curcas against paracetamol-induced acute changes in lipid, atherogenic and haematologic parameters of albino rats were studied. \u0000Methodology: Twenty-five adult male albino rats weighing 180 to 200 g were randomly assigned into 5 experimental groups (I-V) of five animals each. Group I animals were administered 10 ml of distilled water, while group II rats were given 1000 mg/kg paracetamol. Groups III-V were pretreated with vitamin C (500 mg/kg), E. heterophylla (200 mg/kg) and J.curcas (1000 mg/kg) respectively, 1 h before administration of 1000 mg/kg paracetamol. The animals were orally administered the extracts/drugs daily for 14 days. \u0000Result: Paracetamol administration reduced significantly (p 0.05) countered the observed effects of paracetamol overdose more than the extracts of E. heterophylla and J.curcas. Acute paracetamol overdose did not significantly (p>0.05) affect most of the atherogenic risk predictor indices and haematological parameters studied. \u0000Conclusion: The results indicate that atherogenic and haematologic indices were less responsive than lipid parameters to paracetamol-induced toxicity. Furthermore, aqueous leaf extracts of E. heterophylla and J.curcas had less protective effect than vitamin C against serum lipidaemic changes induced by paracetamol.","PeriodicalId":15433,"journal":{"name":"Journal of Clinical Toxicology","volume":"15 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80969065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Use of an Amphoteric Solution in Eye, Skin and Oral Chemical Exposures:Retrospective Multicenter Clinical Case Series 两性溶液在眼睛、皮肤和口腔化学暴露中的使用:回顾性多中心临床病例系列

Journal of Clinical Toxicology Pub Date : 2017-03-27 DOI: 10.4172/2161-0495.1000343

Fortin Jl, Fontaine M, Bodson L, Depil Duvala A, Bitar Mp, Macher Jm, Paulin P, Ravat F, Hall Ah

{"title":"Use of an Amphoteric Solution in Eye, Skin and Oral Chemical Exposures:Retrospective Multicenter Clinical Case Series","authors":"Fortin Jl, Fontaine M, Bodson L, Depil Duvala A, Bitar Mp, Macher Jm, Paulin P, Ravat F, Hall Ah","doi":"10.4172/2161-0495.1000343","DOIUrl":"https://doi.org/10.4172/2161-0495.1000343","url":null,"abstract":"Introduction: A polyvalent amphoteric flushing solution (Diphoterine®) has been in use for a number of years, mainly in industrial settings for decontamination of acid, base, and other corrosive or irritant substances eye and skin splashes. Methods: Retrospective collection of 34 cases from several centers reporting use of Diphoterine® decontamination of eye, skin or oral chemical exposures. The following data were retrieved: exposure circumstances (workplace, domestic, deliberate assault), chemical nature and pH, exposure type, initial clinical signs, clinical signs after flushing, initial and final visual analog scale (VAS) pain ratings, consulting specialist physicians’ conclusions. Results: 58.8% of the 34 cases were occupational exposures, 29.4% were domestic, 5.9% occurred in schools, and 5.9% were deliberate chemical assaults. Of involved chemicals, 11 were basic substances, 11 were acidic, 1 was an oxidizing substance, 2 were solvents, and 9 were miscellaneous substances. There were 21 ocular exposures, 8 cutaneous exposures, 4 mixed (ocular/cutaneous), and 1 oral exposure. Initial clinical findings in ocular exposures were: pain, blepharospasm, hyperemia, palpebral edema, excessive tearing, and blurred vision. Of cutaneous exposures, 1 was a deep necrotic injury and 7 were superficial. Median (IQR) VAS before flushing with Diphoterine® was 7; VAS after ocular or skin flushing was 1. Conclusion: Early application of the amphoteric solution to the eye or skin reduces the intensity of pain associated with chemical injury. While randomized clinical trials are lacking, early use of the amphoteric solution appears to reduce the incidence of sequelae.","PeriodicalId":15433,"journal":{"name":"Journal of Clinical Toxicology","volume":"13 4 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2017-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85265267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Severe Nervous System Damage in Long-Term Professional Exposure toPhthalates 长期专业接触邻苯二甲酸盐对神经系统的严重损害

Journal of Clinical Toxicology Pub Date : 2017-03-20 DOI: 10.4172/2167-7972.1000342

W. Kuliński

引用次数: 1

Accidental Ethanol Ingestion in a 32 Day Old Infant 32日龄婴儿意外摄入乙醇

Journal of Clinical Toxicology Pub Date : 2017-03-17 DOI: 10.4172/2161-0495.1000341

N. Sankhla, P. Schneider, P. Ghosh

引用次数: 0

Salvia Divinorum: A Case Report 鼠尾草:一例报告

Journal of Clinical Toxicology Pub Date : 2017-03-13 DOI: 10.4172/2167-7972.1000340

Feridun Gürlek, Eyyüp TaÅdemir, T. Teker

引用次数: 1

Discovering Capacity in Novel Targets: C. Elegans as a Model Life Form in Toxicological Research 发现新目标的能力:秀丽隐杆线虫作为毒理学研究中的模式生命形式

Journal of Clinical Toxicology Pub Date : 2017-02-24 DOI: 10.4172/2161-0495.1000339

Destefani Ac, Costa Ds, Zanardo Tec, Taufner Gh

引用次数: 2