Cytotoxicity Studies of Functionalized Gold Nanoparticles Using Yeast CometAssay

Abstract

In the present study gold nanoparticles and glucose capped gold nanoparticles are synthesized by chemical route method and characterized using UV-SPR, FTIR and TEM analysis. Single cell gel electrophoresis (SCGE) assay was used to study DNA damage. Studies show that glucose capped gold nanoparticles are less toxic as compare to gold nanoparticles at DNA level. Somewhat larger gold nanoparticle used to monitor endocytosis in log-phase S. cervisiae spheroplasts at 10 to 30 μM was not reported to cause growth inhibition. It shows that glucose capped gold nanoparticles are nontoxic to yeast strain D7. DNA damage was observed by using standard method called Yeast comet assay, which provides a very sensitive method for detecting strand breaks and repair kinetics in single cells. Studies showed that 5 μM-30 μM having very less sign of DNA damage in case of Glucose capped gold nanoparticles and it also shows toxic effect for without glucose capped gold nanoparticles. OTM for different concentration as shown in the image and OTM with respect to different concentration shows the DNA damage, these studies also correlated with survival studies.