Journal of Comparative Physiology B最新文献

{"title":"Management of inorganic elements by overwintering physiology of cold hardy larvae of European corn borer (Ostrinia nubilalis, Hbn.)","authors":"","doi":"10.1007/s00360-024-01537-5","DOIUrl":"https://doi.org/10.1007/s00360-024-01537-5","url":null,"abstract":"<h3>Abstract</h3> <p>The European corn borer (<em>Ostrinia nubilalis</em>, Hbn.), enters diapause, a strategy characterized by arrest of development and reproduction, reduction of metabolic rate and the emergence of increased resistance to challenging seasonal conditions as low sub-zero winter temperatures. The aim of this study was to investigate the potential role of inorganic elements in the ecophysiology of <em>O. nubilalis</em>, analysing their content in the whole body, hemolymph and fat body, both metabolically active, non-diapausing and overwintering diapausing larvae by ICP-OES spectrometer following the US EPA method 200.7:2001. <em>O nubilalis</em> as many phytophagous lepidopteran species maintain a very low extracellular sodium concentration and has potassium as dominant cation in hemolymph of their larvae. Changes in hemolymph and the whole body sodium content occur already at the onset of diapause (when the mean environmental temperatures are still high above 0 ºC) and remain stable during the time course of diapause when larvae of this species cope with sub-zero temperatures, it seems that sodium content regulation is rather a part of diapausing program than the direct effect of exposure to low temperatures. Compared to non-diapausing <em>O. nubilalis</em> larvae, potassium levels are much higher in the whole body and fat body of diapausing larvae and substantially increase approaching the end of diapause. The concentration of Ca, Mg, P and S differed in the whole body, hemolymph and fat body between non-diapausing and diapausing larvae without a unique trend during diapause, except an increase in their contents at the end of diapause.</p>","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140116200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Telomere dynamics during hibernation in a tropical primate","authors":"M. B. Blanco, D. L. Smith, L. K. Greene, A. D. Yoder, E. E. Ehmke, J. Lin, P. H. Klopfer","doi":"10.1007/s00360-024-01541-9","DOIUrl":"https://doi.org/10.1007/s00360-024-01541-9","url":null,"abstract":"<p>Hibernation is a widespread metabolic strategy among mammals for surviving periods of food scarcity. During hibernation, animals naturally alternate between metabolically depressed torpor bouts and energetically expensive arousals without ill effects. As a result, hibernators are promising models for investigating mechanisms that buffer against cellular stress, including telomere protection and restoration. In non-hibernators, telomeres, the protective structural ends of chromosomes, shorten with age and metabolic stress. In temperate hibernators, however, telomere shortening and elongation can occur in response to changing environmental conditions and associated metabolic state. We investigate telomere dynamics in a tropical hibernating primate, the fat-tailed dwarf lemur (<i>Cheirogaleus medius</i>). In captivity, these lemurs can hibernate when maintained under cold temperatures (11–15 °C) with limited food provisioning. We study telomere dynamics in eight fat-tailed dwarf lemurs at the Duke Lemur Center, USA, from samples collected before, during, and after the hibernation season and assayed via qPCR. Contrary to our predictions, we found that telomeres were maintained or even lengthened during hibernation, but shortened immediately thereafter. During hibernation, telomere lengthening was negatively correlated with time in euthermia. Although preliminary in scope, our findings suggest that there may be a preemptive, compensatory mechanism to maintain telomere integrity in dwarf lemurs during hibernation. Nevertheless, telomere shortening immediately afterward may broadly result in similar outcomes across seasons. Future studies could profitably investigate the mechanisms that offset telomere shortening within and outside of the hibernation season and whether those mechanisms are modulated by energy surplus or crises.</p>","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140098953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of sodium–glucose cotransporter-1 activities in maintaining oscillatory Cl− currents from mouse submandibular acinar cells","authors":"","doi":"10.1007/s00360-024-01532-w","DOIUrl":"https://doi.org/10.1007/s00360-024-01532-w","url":null,"abstract":"<h3>Abstract</h3> <p>In salivary acinar cells, cholinergic stimulation induces elevations of cytosolic [Ca²⁺]_i to activate the apical exit of Cl⁻ through TMEM16A Cl⁻ channels, which acts as a driving force for fluid secretion. To sustain the Cl⁻ secretion, [Cl⁻]_i must be maintained to levels that are greater than the electrochemical equilibrium mainly by Na⁺-K⁺-2Cl⁻ cotransporter-mediated Cl⁻ entry in basolateral membrane. Glucose transporters carry glucose into the cytoplasm, enabling the cells to produce ATP to maintain Cl⁻ and fluid secretion. Sodium–glucose cotransporter-1 is a glucose transporter highly expressed in acinar cells. The salivary flow is suppressed by the sodium–glucose cotransporter-1 inhibitor phlorizin. However, it remains elusive how sodium–glucose cotransporter-1 contributes to maintaining salivary fluid secretion. To examine if sodium–glucose cotransporter-1 activity is required for sustaining Cl⁻ secretion to drive fluid secretion, we analyzed the Cl⁻ currents activated by the cholinergic agonist, carbachol, in submandibular acinar cells while comparing the effect of phlorizin on the currents between the whole-cell patch and the gramicidin-perforated patch configurations. Phlorizin suppressed carbachol-induced oscillatory Cl⁻ currents by reducing the Cl⁻ efflux dependent on the Na⁺-K⁺-2Cl⁻ cotransporter-mediated Cl⁻ entry in addition to affecting TMEM16A activity. Our results suggest that the sodium–glucose cotransporter-1 activity is necessary for maintaining the oscillatory Cl⁻ secretion supported by the Na⁺-K⁺-2Cl⁻ cotransporter activity in real time to drive fluid secretion. The concerted effort of sodium–glucose cotransporter-1, Na⁺-K⁺-2Cl⁻ cotransporter, and apically located Cl⁻ channels might underlie the efficient driving of Cl⁻ secretion in different secretory epithelia from a variety of animal species.</p>","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139664451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Na⁺/HCO₃^- cotransporter 1 (nbce1) isoform gene expression during smoltification and seawater acclimation of Atlantic salmon.","authors":"Jason P Breves, Ian S McKay, Victor Koltenyuk, Nastasia N Nelson, Sean C Lema, Stephen D McCormick","doi":"10.1007/s00360-022-01443-8","DOIUrl":"10.1007/s00360-022-01443-8","url":null,"abstract":"<p><p>The life history of Atlantic salmon (Salmo salar) includes an initial freshwater phase (parr) that precedes a springtime migration to marine environments as smolts. The development of osmoregulatory systems that will ultimately support the survival of juveniles upon entry into marine habitats is a key aspect of smoltification. While the acquisition of seawater tolerance in all euryhaline species demands the concerted activity of specific ion pumps, transporters, and channels, the contributions of Na⁺/HCO₃^- cotransporter 1 (Nbce1) to salinity acclimation remain unresolved. Here, we investigated the branchial and intestinal expression of three Na⁺/HCO₃^- cotransporter 1 isoforms, denoted nbce1.1, -1.2a, and -1.2b. Given the proposed role of Nbce1 in supporting the absorption of environmental Na⁺ by ionocytes, we first hypothesized that expression of a branchial nbce1 transcript (nbce1.2a) would be attenuated in salmon undergoing smoltification and following seawater exposure. In two separate years, we observed spring increases in branchial Na⁺/K⁺-ATPase activity, Na⁺/K⁺/2Cl^- cotransporter 1, and cystic fibrosis transmembrane regulator 1 expression characteristic of smoltification, whereas there were no attendant changes in nbce1.2a expression. Nonetheless, branchial nbce1.2a levels were reduced in parr and smolts within 2 days of seawater exposure. In the intestine, gene transcript abundance for nbce1.1 increased from spring to summer in the anterior intestine, but not in the posterior intestine or pyloric caeca, and nbce1.1 and -1.2b expression in the intestine showed season-dependent transcriptional regulation by seawater exposure. Collectively, our data indicate that tissue-specific modulation of all three nbce1 isoforms underlies adaptive responses to seawater.</p>","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"2 1","pages":"577-592"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73998150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retention of larval skin traits in adult amphibious killifishes: a cross-species investigation.","authors":"Louise Tunnah, Jonathan M Wilson, Patricia A Wright","doi":"10.1007/s00360-022-01436-7","DOIUrl":"10.1007/s00360-022-01436-7","url":null,"abstract":"<p><p>The gills are the primary site of exchange in fishes. However, during early life-stages or in amphibious fishes, ionoregulation and gas-exchange may be primarily cutaneous. Given the similarities between larval and amphibious fishes, we hypothesized that cutaneous larval traits are continuously expressed in amphibious fishes across all life-stages to enable the skin to be a major site of exchange on land. Alternatively, we hypothesized that cutaneous larval traits disappear in juvenile stages and are re-expressed in amphibious species in later life-stages. We surveyed six species spanning a range of amphibiousness and characterized cutaneous ionocytes and neuroepithelial cells (NECs) as representative larval skin traits at up to five stages of development. We found that skin ionocyte density remained lower and constant in exclusively water-breathing, relative to amphibious species across development, whereas in amphibious species ionocyte density generally increased. Additionally, adults of the most amphibious species had the highest cutaneous ionocyte densities. Surprisingly, cutaneous NECs were only identified in the skin of one amphibious species (Kryptolebias marmoratus), suggesting that cutaneous NECs are not a ubiquitous larval or amphibious skin trait, at least among the species we studied. Our data broadly supports the continuous-expression hypothesis, as three of four amphibious experimental species expressed cutaneous ionocytes in all examined life-stages. Further, the increasing density of cutaneous ionocytes across development in amphibious species probably facilitates the prolonged occupation of terrestrial habitats.</p>","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"31 1","pages":"473-488"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87886294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative damages and antioxidant defences after feeding a single meal in rainbow trout.","authors":"N Schvezov, R W Wilson, M A Urbina","doi":"10.1007/s00360-022-01435-8","DOIUrl":"10.1007/s00360-022-01435-8","url":null,"abstract":"<p><p>Feeding and digestion are metabolically demanding causing a rise on metabolic rate called Specific Dynamic Action (SDA). Although SDA has been vastly reported in fish, its potential consequences on the oxidative-antioxidant balance has not been evaluated to date in fish, a model with a long alkaline tide associated with feeding as well. Using rainbow trout (Oncorhynchus mykiss) as a model species, the aims of the present study were to: (1) assess potential oxidative damages and changes in oxidative defences after feeding on a single meal, and (2) identify the timescale of such changes over a 96 h post-feeding period. Oxidative damage in proteins and lipids and the activities of four enzymatic antioxidant defences: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) were measured in gill, stomach, intestine and liver. DNA damage was measured in red blood cells. Fish were sampled before and after 1.5, 6, 24, 48, 72 and 96 h of ingestion of a 3% body mass ration. Trends of post-prandial damage were present in all tissues, but only protein oxidation varied significatively during digestion in the stomach. The intestine and stomach presented the highest enzymatic activities, likely due to the high metabolic action that these tissues have during digestion, with peaks during post-feeding: at 24 h of SOD in stomach and at 48 h of CAT in intestine. Observed GPx peaks during post-feeding in gills are likely due to the exacerbated demands for ion fluxes and/or oxygen during feeding. The differential response of the antioxidant system observed in tissues of rainbow trout during digestion indicates a coordinated and tissue-specific antioxidant defence.</p>","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"18 1","pages":"459-471"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90663836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between gene expression networks and muscle contractile physiology differences in Anolis lizards.","authors":"Luke B Smith, Christopher V Anderson, Miyuraj H Hikkaduwa Withangage, Andrew Koch, Thomas J Roberts, Andrea L Liebl","doi":"10.1007/s00360-022-01441-w","DOIUrl":"10.1007/s00360-022-01441-w","url":null,"abstract":"<p><p>Muscles facilitate most animal behavior, from eating to fleeing. However, to generate the variation in behavior necessary for survival, different muscles must perform differently; for instance, sprinting requires multiple rapid muscle contractions, whereas biting may require fewer contractions but greater force. Here, we use a transcriptomic approach to identify genes associated with variation in muscle contractile physiology among different muscles from the same individual. We measured differential gene expression between a leg and jaw muscle of Anolis lizards known to differ in muscle contractile physiology and performance. For each individual, one muscle was used to measure muscle contractile physiology, including contractile velocity (V_max and V₄₀), specific tension, power ratio, and twitch time, whereas the contralateral muscle was used to extract RNA for transcriptomic sequencing. Using the transcriptomic data, we found clear clustering of muscle type. Expression of genes clustered in gene ontology (GO) terms related to muscle contraction and extracellular matrix was, on average, negatively correlated with V_max and slower twitch times but positively correlated to power ratio and V₄₀. Conversely, genes related to the GO terms related to aerobic respiration were downregulated in muscles with higher power ratio and V₄₀, and over-expressed as twitch time decreased. Determining the molecular mechanisms that underlie variation in muscle contractile physiology can begin to explain how organisms are able to optimize behavior under variable conditions. Future studies pursuing the effects of differential gene expression across muscle types in different environments might inform researchers about how differences develop across species, populations, and individuals varying in ecological history.</p>","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"36 1","pages":"489-499"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84371480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Prejuveniles of Mugil liza (Actinopterygii; Fam. Mugilidae) show digestive and metabolic flexibility upon different postprandial times and refeeding","authors":"Camila Albanesi, M. González-Castro, Alejandra López-Mañanes","doi":"10.1007/s00360-022-01445-6","DOIUrl":"https://doi.org/10.1007/s00360-022-01445-6","url":null,"abstract":"","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"1 1","pages":"575 - 575"},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88786394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Season and sex have different effects on hematology and cytokines in striped hamsters (Cricetulus barabensis).","authors":"De-Li Xu, Xiao-Kai Hu","doi":"10.1007/s00360-019-01246-4","DOIUrl":"10.1007/s00360-019-01246-4","url":null,"abstract":"<p><p>Animals in the temperate zones face seasonal variations in environments and hence their immune responses change seasonally. In the current study, seasonal changes in hematological parameters and cytokines in striped hamsters (Cricetulus barabensis) were examined to test the winter immunoenhancement hypothesis, which states that immune function tends to increase in fall and winter compared with other seasons. Male and female hamsters were captured from the wild in the fall and winter of 2014 and in the spring and summer of 2015. Maximum body mass in both sexes and relative fatness in female hamsters occurred in the summer, indicating that body condition was the best during this season. All hematological parameters were not different between male and female hamsters, and were also not affected by the interaction of season and sex except neutrophil granulocytes (GRAN). Red blood cells (RBC) and haematocrit (PCV) were higher in the fall and winter, and hemoglobin concentration (HGB) was the highest in winter in hamsters compared with the spring and summer, implying that their oxygen-carrying capacity and oxygen affinity of the blood increased during these seasons. Compared with other seasons, the number of white blood cells (WBC) was higher in winter than in summer, intermediate granulocytes (MID), the percent of MID (MID%), GRAN and the percent of GRAN (GRAN%) were the highest in winter, which all supported the winter immunoenhancement hypothesis. However, the count of lymphocytes (LYMF) was the highest in spring, being inconsistent with this hypothesis. IL-2 levels, but not TNF-α, were influenced by seasons, sex and their interaction in hamsters. Regardless of sex, IL-4 titres were higher in spring and summer than in fall and winter in hamsters. INF-γ titres in male hamsters did not differ between the spring and summer, while its titres in female hamsters was lower in spring in contrast with winter and summer. Higher IL-2 and IL-4 levels during the breeding seasons might be crucial in controlling the increased possibilities of infections in these seasons. In summary, season and sex had disparate effects on different hematological profiles and the levels of cytokines in hamsters.</p>","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"5 1","pages":"87-100"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74604649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analyses provide the first insight into the molecular basis of cold tolerance in Larimichthys polyactis","authors":"Feng Liu, T. Chu, Mengjie Wang, W. Zhan, Qing-Ping Xie, Bao Lou","doi":"10.1007/s00360-019-01247-3","DOIUrl":"https://doi.org/10.1007/s00360-019-01247-3","url":null,"abstract":"","PeriodicalId":15377,"journal":{"name":"Journal of Comparative Physiology B","volume":"5 1","pages":"27 - 34"},"PeriodicalIF":0.0,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81919616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}