热带灵长类动物冬眠期间端粒的动态变化

M. B. Blanco, D. L. Smith, L. K. Greene, A. D. Yoder, E. E. Ehmke, J. Lin, P. H. Klopfer
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引用次数: 0

摘要

冬眠是哺乳动物普遍采用的一种新陈代谢策略,用以度过食物匮乏期。在冬眠过程中,动物会自然地在新陈代谢低下的冬眠期和能量消耗高的唤醒期之间交替,而不会产生不良影响。因此,冬眠动物有望成为研究细胞压力缓冲机制(包括端粒保护和恢复)的模型。在非冬眠动物中,染色体的保护性结构端粒会随着年龄的增长和新陈代谢的压力而缩短。然而,在温带冬眠动物中,端粒的缩短和伸长会随着环境条件和相关代谢状态的变化而发生。我们研究了一种热带冬眠灵长类动物--肥尾矮狐猴(Cheirogaleus medius)的端粒动态。在人工饲养条件下,这些狐猴可以在低温(11-15 °C)和有限的食物供给条件下冬眠。我们研究了美国杜克狐猴中心的八只胖尾矮狐猴的端粒动态,这些端粒是在冬眠季节之前、期间和之后采集的,并通过 qPCR 进行了检测。与我们的预测相反,我们发现端粒在冬眠期间保持不变甚至延长,但在冬眠后立即缩短。在冬眠期间,端粒的延长与休眠时间呈负相关。我们的研究结果虽然只是初步的,但它表明矮狐猴在冬眠期间可能存在一种先发制人的补偿机制来维持端粒的完整性。然而,紧接着的端粒缩短可能会在不同季节造成类似的结果。未来的研究可以探索在冬眠季节内外抵消端粒缩短的机制,以及这些机制是否会受到能量过剩或危机的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Telomere dynamics during hibernation in a tropical primate

Telomere dynamics during hibernation in a tropical primate

Hibernation is a widespread metabolic strategy among mammals for surviving periods of food scarcity. During hibernation, animals naturally alternate between metabolically depressed torpor bouts and energetically expensive arousals without ill effects. As a result, hibernators are promising models for investigating mechanisms that buffer against cellular stress, including telomere protection and restoration. In non-hibernators, telomeres, the protective structural ends of chromosomes, shorten with age and metabolic stress. In temperate hibernators, however, telomere shortening and elongation can occur in response to changing environmental conditions and associated metabolic state. We investigate telomere dynamics in a tropical hibernating primate, the fat-tailed dwarf lemur (Cheirogaleus medius). In captivity, these lemurs can hibernate when maintained under cold temperatures (11–15 °C) with limited food provisioning. We study telomere dynamics in eight fat-tailed dwarf lemurs at the Duke Lemur Center, USA, from samples collected before, during, and after the hibernation season and assayed via qPCR. Contrary to our predictions, we found that telomeres were maintained or even lengthened during hibernation, but shortened immediately thereafter. During hibernation, telomere lengthening was negatively correlated with time in euthermia. Although preliminary in scope, our findings suggest that there may be a preemptive, compensatory mechanism to maintain telomere integrity in dwarf lemurs during hibernation. Nevertheless, telomere shortening immediately afterward may broadly result in similar outcomes across seasons. Future studies could profitably investigate the mechanisms that offset telomere shortening within and outside of the hibernation season and whether those mechanisms are modulated by energy surplus or crises.

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