Journal of Chemical Biology最新文献_第4页

Photochemical synthesis and anticancer activity of barbituric acid, thiobarbituric acid, thiosemicarbazide, and isoniazid linked to 2-phenyl indole derivatives. 巴比妥酸、硫代巴比妥酸、硫代氨基脲和2-苯基吲哚衍生物异烟肼的光化学合成及其抗癌活性。

Journal of Chemical Biology Pub Date : 2015-11-17 eCollection Date: 2016-04-01 DOI: 10.1007/s12154-015-0148-y

S Vijaya Laxmi, G Rajitha, B Rajitha, Asha Jyothi Rao

引用次数: 16

Probing the future of correlative microscopy 探讨相关显微术的未来

Journal of Chemical Biology Pub Date : 2015-10-10 DOI: 10.1007/S12154-015-0147-Z

L. Collinson, P. Verkade

引用次数: 4

Standard fluorescent proteins as dual-modality probes for correlative experiments in an integrated light and electron microscope 标准荧光蛋白在集成光镜和电子显微镜下作为相关实验的双模探针

Journal of Chemical Biology Pub Date : 2015-07-08 DOI: 10.1007/S12154-015-0143-3

E. Brama, C. Peddie, Martin L. Jones, M. Domart, X. Snetkov, M. Way, Banafshé Larijani, L. Collinson

引用次数: 15

Multicolour correlative imaging using phosphor probes 利用荧光粉探针进行多色相关成像

Journal of Chemical Biology Pub Date : 2015-06-20 DOI: 10.1007/S12154-015-0141-5

I. Morrison, Alireza Samilian, P. Coppo, T. Ireland, G. Fern, J. Silver, R. Withnall, P. O'Toole

引用次数: 13

JOCB Bulletin. JOCB公告。

Journal of Chemical Biology Pub Date : 2015-06-09 eCollection Date: 2015-07-01 DOI: 10.1007/s12154-015-0136-2

引用次数: 0

Steroidal dihydrocarbothioic acid amido pyrazoles: synthesis, characterization, cytotoxicity and genotoxicity studies. 甾体二氢碳硫酸氨基吡唑:合成、表征、细胞毒性和遗传毒性研究。

Journal of Chemical Biology Pub Date : 2015-06-05 eCollection Date: 2015-07-01 DOI: 10.1007/s12154-015-0137-1

Ayaz Mahmood Dar, Manzoor Ahmad Gatoo, Shamsuzzaman

{"title":"Steroidal dihydrocarbothioic acid amido pyrazoles: synthesis, characterization, cytotoxicity and genotoxicity studies.","authors":"Ayaz Mahmood Dar, Manzoor Ahmad Gatoo, Shamsuzzaman","doi":"10.1007/s12154-015-0137-1","DOIUrl":"https://doi.org/10.1007/s12154-015-0137-1","url":null,"abstract":"A new series of steroidal dihydrocarbothioic acid amido pyrazole analogues were synthesized, and after characterization, evaluation for cytotoxicity, comet assay and western blotting was carried out. The synthesis of these analogues is convenient and involves two steps, i.e. aldol condensation as first step followed by nucleophilic addition of thiosemicarbazide across α, β-unsaturated carbonyl as a later step. Quantitative yields of more than 80 % are obtained in both the steps. After characterization by IR, (1)H NMR, (13)C NMR, MS and analytical data, all the compounds of both series were tested for cytotoxic activity against a panel of different human cancer cell lines by MTT assay, during which compound 3e, 3f, 4e, 4f and 4h are very potent especially against HepG2 and MCF-7 cancer cell lines. Cell cycle analysis depicted the cell death in S-phase while as annexin V-FITC/PI analysis showed that compounds effectively induce apoptosis. Apoptotic degradation of DNA of MCF-7 cells in the presence of different steroidal derivatives was analysed by agarose gel electrophoresis and visualized by ethidium bromide staining (comet assay). In western blotting analysis, the relative expressions of relevant apoptotic markers depicted an apoptosis by steroidal dihydropyrazole in MCF-7 cancer cells. ","PeriodicalId":15296,"journal":{"name":"Journal of Chemical Biology","volume":"8 3","pages":"107-18"},"PeriodicalIF":0.0,"publicationDate":"2015-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12154-015-0137-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33412585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The potential of bioorthogonal chemistry for correlative light and electron microscopy: a call to arms 生物正交化学在相关光学和电子显微镜上的潜力:战斗的召唤

Journal of Chemical Biology Pub Date : 2015-05-26 DOI: 10.1007/S12154-015-0134-4

D. M. Elsland, E. Bos, H. Overkleeft, A. Koster, S. I. Kasteren

引用次数: 3

Multimodal correlative microscopy for in situ detection and quantification of chemical elements in biological specimens. Applications to nanotoxicology 用于生物标本中化学元素原位检测和定量的多模态相关显微镜。纳米毒理学的应用

Journal of Chemical Biology Pub Date : 2015-05-22 DOI: 10.1007/S12154-015-0133-5

Q. L. Trequesser, G. Saez, M. Simón, G. Devès, L. Daudin, P. Barberet, C. Michelet, M. Delville, H. Seznec

引用次数: 2

Prediction of protein targets of kinetin using in silico and in vitro methods: a case study on spinach seed germination mechanism. 用计算机和体外方法预测动素蛋白靶点:以菠菜种子萌发机制为例。

Journal of Chemical Biology Pub Date : 2015-05-12 eCollection Date: 2015-07-01 DOI: 10.1007/s12154-015-0135-3

Sivakumar Prasanth Kumar, Vilas R Parmar, Yogesh T Jasrai, Himanshu A Pandya

{"title":"Prediction of protein targets of kinetin using in silico and in vitro methods: a case study on spinach seed germination mechanism.","authors":"Sivakumar Prasanth Kumar, Vilas R Parmar, Yogesh T Jasrai, Himanshu A Pandya","doi":"10.1007/s12154-015-0135-3","DOIUrl":"https://doi.org/10.1007/s12154-015-0135-3","url":null,"abstract":"Kinetin, a cytokinin which promotes seed germination by inhibiting the action of abscisic acid, is an important molecule known to trigger various molecular mechanisms by interacting with an array of proteins shown from experimental observations in various model organisms. We report here the prediction of most probable protein targets of kinetin from spinach proteome using in silico approaches. Inverse docking and ligand-based similarity search was performed using kinetin as molecule. The former method prioritized six spinach proteins, whereas the latter method provided a list of protein targets retrieved from several model organisms. The most probable protein targets were selected by comparing the rank list of docking and ligand similarity methods. Both of these methods prioritized chitinase as the most probable protein target (ΔG pred = 5.064 kcal/mol) supported by the experimental structure of yeast chitinase 1 complex with kinetin (PDB: 2UY5) and Gliocladium roseum chitinase complex with 3,7-dihydro-1,3,7-trimethyl-1H-purine-2,6-dione (caffeine; 3G6M) which bears a 3D similarity of 0.43 with kinetin. An in vitro study to evaluate the effect of kinetin on spinach seed germination indicated that a very low concentration of kinetin (0.5 mg/l) did not show a significant effect compared to control in inducing seed germination process. Further, higher levels of kinetin (>0.5 mg/l) constituted an antagonist effect on spinach seed germination. It is anticipated that kinetin may have a molecular interaction with prioritized protein targets synthesized during the seed germination process and reduces growth. Thus, it appears that kinetin may not be a suitable hormone for enhancing spinach seed germination in vitro. ","PeriodicalId":15296,"journal":{"name":"Journal of Chemical Biology","volume":"8 3","pages":"95-105"},"PeriodicalIF":0.0,"publicationDate":"2015-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12154-015-0135-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33412584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Advances in molecular probe-based labeling tools and their application to multiscale multimodal correlated microscopies 基于分子探针的标记工具及其在多尺度多模态相关显微镜中的应用进展

Journal of Chemical Biology Pub Date : 2015-05-01 DOI: 10.1007/S12154-015-0132-6

Mark Ellisman, T. Deerinck, K. Kim, E. Bushong, S. Phan, A. Ting, D. Boassa

引用次数: 5