Journal of biochemical and biophysical methods最新文献_第2页

12th International Symposium of Chemistry, Miercurea Ciuc (Csíkszereda), Romania, 5th to 8th of October, 2006. 第12届国际化学研讨会，Miercurea Ciuc（Csíkszereda），罗马尼亚，2006年10月5日至8日。

Journal of biochemical and biophysical methods Pub Date : 2008-04-24 DOI: 10.1016/j.jprot.2007.11.012

Ferenc Kilár

引用次数: 0

Controlled release of preservatives using dealuminated zeolite Y 用Y型脱铝沸石控制防腐剂的释放

Journal of biochemical and biophysical methods Pub Date : 2008-04-24 DOI: 10.1016/j.jbbm.2007.05.010

Håkan Eriksson

引用次数: 10

Determination of zinc in vegetal tissue microsamples by platinum-wire loop in flame atomization atomic absorption spectrometry 火焰雾化原子吸收法测定植物组织微量样品中的锌

Journal of biochemical and biophysical methods Pub Date : 2008-04-24 DOI: 10.1016/j.jbbm.2007.04.006

Ladislau Kékedy-Nagy , Yao Jun , Eugen Darvasi , László Kékedy-Nagy Jr.

{"title":"Determination of zinc in vegetal tissue microsamples by platinum-wire loop in flame atomization atomic absorption spectrometry","authors":"Ladislau Kékedy-Nagy , Yao Jun , Eugen Darvasi , László Kékedy-Nagy Jr.","doi":"10.1016/j.jbbm.2007.04.006","DOIUrl":"10.1016/j.jbbm.2007.04.006","url":null,"abstract":"<div><p>The zinc content of 3 μL of vegetal samples (tree leaves, lichens and grape sap) atomized from a Pt-wire in the methane–air flame has been determined by atomic absorption spectrometry. The effect of gas flow rates and the atomization height in the flame on the absorption of zinc was evaluated at 213.9 nm. The best results were obtained at a height of 5 mm and gas flow rates of 200 L/h air and 26 L/h methane, respectively. The effect of Na, K, Ca, Mg, SO<sub>4</sub><sup>2−</sup>, and PO<sub>4</sub><sup>3−</sup> on the absorption of zinc was studied too. The detection limit of 0.40±0.21 ng was obtained at a significance level of 0.05, using the two-step Neyman–Pearson criterion. The zinc content of the samples has been determined with continuous nebulization and by atomization from the Pt-wire, using both the standard calibration curve and the standard addition method. The results of the two procedures agree within the determination errors.</p></div>","PeriodicalId":15257,"journal":{"name":"Journal of biochemical and biophysical methods","volume":"70 6","pages":"Pages 1234-1239"},"PeriodicalIF":0.0,"publicationDate":"2008-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jbbm.2007.04.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26807454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

PCR-free mutation detection of BRCA1 on a zip-code microarray using ligase chain reaction 利用连接酶链反应在邮政编码微阵列上检测BRCA1的无pcr突变

Journal of biochemical and biophysical methods Pub Date : 2008-04-24 DOI: 10.1016/j.jprot.2008.01.005

Agnishwar Girigoswami, Cheulhee Jung, Hyo Young Mun, Hyun Gyu Park

{"title":"PCR-free mutation detection of BRCA1 on a zip-code microarray using ligase chain reaction","authors":"Agnishwar Girigoswami, Cheulhee Jung, Hyo Young Mun, Hyun Gyu Park","doi":"10.1016/j.jprot.2008.01.005","DOIUrl":"10.1016/j.jprot.2008.01.005","url":null,"abstract":"<div><p>We describe here ligation-based strategy to detect mutations in <em>BRCA1</em> utilizing zip-code microarray technology. In our first approach, PCR was performed to amplify the genomic regions containing the mutation sites. The PCR products were then used as templates in a subsequent ligation reaction using two ligation primers that flanked the mutation site. The primary allele-specific primer is designed to contain a base of mutation site at its 3′ end with 5′ complementarity to the respective zip-code sequence while the secondary common primer is modified by biotin at its 3′ end. Depending on the genotype of samples at the mutation site, the nick between the two ligation primers can be sealed in the presence of DNA ligase. The ligation products were then hybridized on the zip-code microarray followed by staining with streptavidine-cy3 to generate a fluorescent signal. Using this strategy we successfully genotyped selected Korean-specific mutation sites in exon 11 of <em>BRCA1</em> with a wild type and two heterozygote mutant samples. Furthermore, we also demonstrated that ligase chain reaction using unamplified genomic DNA as direct templates is enough to generate sufficient signals for correct genotypings in a multiplexed manner, verifying first that PCR is not essential for this microarray-based strategy.</p></div>","PeriodicalId":15257,"journal":{"name":"Journal of biochemical and biophysical methods","volume":"70 6","pages":"Pages 897-902"},"PeriodicalIF":0.0,"publicationDate":"2008-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jprot.2008.01.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27265963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

A sensitive amperometric immunosensor for carcinoembryonic antigen detection with porous nanogold film and nano-Au/chitosan composite as immobilization matrix 以多孔纳米金膜和纳米金/壳聚糖复合材料为固定基质的癌胚抗原检测敏感安培免疫传感器

Journal of biochemical and biophysical methods Pub Date : 2008-04-24 DOI: 10.1016/j.jbbm.2007.06.002

Xiulan He, Ruo Yuan, Yaqin Chai, Yintao Shi

{"title":"A sensitive amperometric immunosensor for carcinoembryonic antigen detection with porous nanogold film and nano-Au/chitosan composite as immobilization matrix","authors":"Xiulan He, Ruo Yuan, Yaqin Chai, Yintao Shi","doi":"10.1016/j.jbbm.2007.06.002","DOIUrl":"10.1016/j.jbbm.2007.06.002","url":null,"abstract":"<div><p>A sensitive amperometric immunosensor for carcinoembryonic antigen (CEA) was prepared. Firstly, a porous nano-structure gold (NG) film was formed on glassy carbon electrode (GCE) by electrochemical reduction of HAuCl<sub>4</sub> solution, then nano-Au/Chit composite was immobilized onto the electrode because of its excellent membrane-forming ability, and finally the anti-CEA was adsorbed onto the surface of the bilayer gold nanoparticles to construct an anti-CEA/nano-Au/Chit/NG/GCE immunosensor. The characteristics of the modified electrode at different stages of modification were studied by cyclic voltammetry (CV). The gold colloid, chitosan and nano-Au/Chit were characterized by transmission electron microscopy and UV–vis spectroscopy. In addition, the performances of the immunosensor were studied in detail. The resulting immunosensor offers a high-sensitivity (1310 nA/ng/ml) for the detection of CEA and has good correlation for detection of CEA in the range of 0.2 to 120.0 ng/ml with a detection limit of 0.06 ng/ml estimated at a signal-to-noise ratio of 3. The proposed method can detect the CEA through one-step immunoassay and would be valuable for clinical immunoassay.</p></div>","PeriodicalId":15257,"journal":{"name":"Journal of biochemical and biophysical methods","volume":"70 6","pages":"Pages 823-829"},"PeriodicalIF":0.0,"publicationDate":"2008-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jbbm.2007.06.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26863136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 116

Novel method for preparation of the template DNA and selection of primers to differentiate the material rice cultivars of rice wine by PCR 本文提出了一种新的模板DNA制备方法和引物选择方法，用于黄酒原料品种的PCR鉴别

Journal of biochemical and biophysical methods Pub Date : 2008-04-24 DOI: 10.1016/j.jbbm.2007.07.001

Ken'ichi Ohtsubo, Keitaro Suzuki, Kazutomo Haraguchi, Sumiko Nakamura

{"title":"Novel method for preparation of the template DNA and selection of primers to differentiate the material rice cultivars of rice wine by PCR","authors":"Ken'ichi Ohtsubo, Keitaro Suzuki, Kazutomo Haraguchi, Sumiko Nakamura","doi":"10.1016/j.jbbm.2007.07.001","DOIUrl":"10.1016/j.jbbm.2007.07.001","url":null,"abstract":"<div><p>As many rice wine brewers label the name of the cultivar of the material rice, authentication technology is necessary. The problems are (1) decomposition of DNAs during the fermentation, (2) contamination of DNAs from microorganisms, (3) co-existence of PCR inhibitors, such as polyphenols. The present authors improved the PCR method by (1) lyophilizing and pulverizing the rice wine to concentrate DNAs, (2) decomposition of starches and proteins so as not to inhibit DNA extraction by the use of heat-resistant amylase and proteinase K, (3) purification of the template DNA by the combination of CTAB method and fractional precipitation by 70% EtOH. To prevent the amplification of microorganism's DNAs during PCR, the present authors selected the suitable plant-specific primers. It became possible to prepare the template DNAs for PCR from the rice wine. The sequences of the amplified DNAs by PCR were ascertained to be same with those of material rice. Mislabeling of material rice cultivar was detected by PCR using the commercial rice wine. It became possible to extract and purify the template DNAs for PCR from the rice wine and to differentiate the material rice cultivars by the PCR using the rice wine as a sample.</p></div>","PeriodicalId":15257,"journal":{"name":"Journal of biochemical and biophysical methods","volume":"70 6","pages":"Pages 1020-1028"},"PeriodicalIF":0.0,"publicationDate":"2008-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jbbm.2007.07.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26869806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

A micro-batchwise technique method for rapid reconstitution of functionally active mitochondrial ADP/ATP carrier from Jerusalem artichoke (Helianthus tuberosus L.) tubers 微批量快速重建菊芋块茎中功能活性线粒体ADP/ATP载体的方法

Journal of biochemical and biophysical methods Pub Date : 2008-04-24 DOI: 10.1016/j.jprot.2007.11.003

A. Spagnoletta , A. De Palma , G. Prezioso , V. Scalera

引用次数: 9

Yeast cell surface display system for determination of humoral response to active immunization with a monoclonal antibody against EpCAM 酵母细胞表面显示系统用于测定抗EpCAM单克隆抗体主动免疫的体液应答

Journal of biochemical and biophysical methods Pub Date : 2008-04-24 DOI: 10.1016/j.jprot.2008.01.008

Astrid Dürauer , Eva Berger , Marlene Zandian , Christa Mersich , Manfred Schuster , Hans Loibner , Alois Jungbauer

{"title":"Yeast cell surface display system for determination of humoral response to active immunization with a monoclonal antibody against EpCAM","authors":"Astrid Dürauer , Eva Berger , Marlene Zandian , Christa Mersich , Manfred Schuster , Hans Loibner , Alois Jungbauer","doi":"10.1016/j.jprot.2008.01.008","DOIUrl":"10.1016/j.jprot.2008.01.008","url":null,"abstract":"<div><p>Even though an immunogenic formulation of the murine monoclonal anti-EpCAM (epithelian cell adhesion molecule) antibody Mab 17-1A, has been shown to evoke a strong humoral immune response in both, monkey studies and early clinical trials, conventional anti-EpCAM ELISA could not identify anti-EpCAM immune response in relation to treatment with Mab17-1A. In contrast, usage of cellulose membranes prepared by SPOT technology presenting overlapping EpCAM peptides allowed the unequivocal determination of EpCAM related antibodies present in monkeys sera after immunization with IGN101. Based on such contradictory results, it was of high interest to compare obtained data to a different method for better assessment of their possible interpretation. Therefore, in the present studies, some EpCAM peptides, determined as reactive by binding of IgG isolated from sera of treated monkeys on membranes prepared by SPOT technology, were represented on yeast surface using the pYD1 yeast display vector system. Binding of biotinylated IgG from sera was detected with streptavidin–FITC and quantity of binding was determined by FACS measurement. Though using this completely different method, experiments with pre-immune and immune sera of four monkeys exemplarily are comparable to the results obtained by analysis with synthetic peptide arrays.</p></div>","PeriodicalId":15257,"journal":{"name":"Journal of biochemical and biophysical methods","volume":"70 6","pages":"Pages 1109-1115"},"PeriodicalIF":0.0,"publicationDate":"2008-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jprot.2008.01.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27304912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

HPLC method for determination of verapamil in human plasma after solid-phase extraction 固相萃取法测定人血浆中维拉帕米含量

Journal of biochemical and biophysical methods Pub Date : 2008-04-24 DOI: 10.1016/j.jbbm.2007.09.009

Violeta Ivanova , Dragica Zendelovska , Marina Stefova , Trajče Stafilov

引用次数: 27

Novel fluorescence method for real-time monitoring of nitric oxide dynamics in nanoscale concentration 纳米浓度下一氧化氮动态实时监测的新型荧光方法

Journal of biochemical and biophysical methods Pub Date : 2008-04-24 DOI: 10.1016/j.jbbm.2007.09.005

Oren Chen, Natalya Uzlaner, Zvi Priel, Gertz I. Likhtenshtein

{"title":"Novel fluorescence method for real-time monitoring of nitric oxide dynamics in nanoscale concentration","authors":"Oren Chen, Natalya Uzlaner, Zvi Priel, Gertz I. Likhtenshtein","doi":"10.1016/j.jbbm.2007.09.005","DOIUrl":"10.1016/j.jbbm.2007.09.005","url":null,"abstract":"<div><p>A novel assay was developed for the measurement of nitric oxide. The proposed method is based on fluorescence, using a fluorophore-heme dual functionality probe (FHP). The heme group can serve as an effective NO-trap, due to its very fast reaction with NO and the high stability of the resulting complex. Since the heme is connected with a fluorophore as a part of the FHP dual-functionality probe, the heme can quench the fluorophore fluorescence, under certain conditions, by a singlet–singlet energy transfer mechanism.</p><p>The proposed method was tested using myoglobin covalently modified by a stilbene label. The change in emission intensity of the stilbene fragment, versus an increasing concentration of NO precursors, clearly demonstrated the spectral sensitivity required to monitor the formation of a heme–NO complex in a concentration range of 10 nM–2 μM. Furthermore, the new methodology for NO measurement was also found to be an effective assay using tissues from rabbit and porcine trachea epithelium. The measured NO flux (in an initial time interval) in tissue sample from rabbit trachea epithelia and porcine trachea epithelia is ∼7.9×10<sup>−12</sup> mol/s×g and ∼3.0×10<sup>−12</sup> mol/s×g respectively.</p></div>","PeriodicalId":15257,"journal":{"name":"Journal of biochemical and biophysical methods","volume":"70 6","pages":"Pages 1006-1013"},"PeriodicalIF":0.0,"publicationDate":"2008-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jbbm.2007.09.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41019683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6