A sensitive amperometric immunosensor for carcinoembryonic antigen detection with porous nanogold film and nano-Au/chitosan composite as immobilization matrix

Journal of biochemical and biophysical methods Pub Date : 2008-04-24 DOI:10.1016/j.jbbm.2007.06.002

Xiulan He, Ruo Yuan, Yaqin Chai, Yintao Shi

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引用次数: 116

Abstract

A sensitive amperometric immunosensor for carcinoembryonic antigen (CEA) was prepared. Firstly, a porous nano-structure gold (NG) film was formed on glassy carbon electrode (GCE) by electrochemical reduction of HAuCl₄ solution, then nano-Au/Chit composite was immobilized onto the electrode because of its excellent membrane-forming ability, and finally the anti-CEA was adsorbed onto the surface of the bilayer gold nanoparticles to construct an anti-CEA/nano-Au/Chit/NG/GCE immunosensor. The characteristics of the modified electrode at different stages of modification were studied by cyclic voltammetry (CV). The gold colloid, chitosan and nano-Au/Chit were characterized by transmission electron microscopy and UV–vis spectroscopy. In addition, the performances of the immunosensor were studied in detail. The resulting immunosensor offers a high-sensitivity (1310 nA/ng/ml) for the detection of CEA and has good correlation for detection of CEA in the range of 0.2 to 120.0 ng/ml with a detection limit of 0.06 ng/ml estimated at a signal-to-noise ratio of 3. The proposed method can detect the CEA through one-step immunoassay and would be valuable for clinical immunoassay.

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以多孔纳米金膜和纳米金/壳聚糖复合材料为固定基质的癌胚抗原检测敏感安培免疫传感器

制备了一种灵敏的癌胚抗原(CEA)安培免疫传感器。首先，通过电化学还原HAuCl4溶液在玻璃碳电极(GCE)上形成多孔纳米结构金(NG)膜，然后利用纳米au /Chit复合材料优异的成膜能力将其固定在电极上，最后将抗cea吸附在双层金纳米颗粒表面，构建抗cea /纳米au /Chit/NG/GCE免疫传感器。利用循环伏安法(CV)研究了改性电极在不同修饰阶段的特性。采用透射电镜和紫外可见光谱对金胶体、壳聚糖和纳米金/金进行了表征。此外，还对免疫传感器的性能进行了详细的研究。所得免疫传感器对CEA的检测具有高灵敏度(1310 nA/ng/ml)，对CEA在0.2 ~ 120.0 ng/ml范围内的检测具有良好的相关性，在信噪比为3的情况下，检测限为0.06 ng/ml。该方法可通过一步免疫分析法检测CEA，具有一定的临床应用价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of biochemical and biophysical methods

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