发布求助
文献互助 智能选刊 最新文献

蚌埠医学院学报最新文献

筛选
英文 中文
碱提酸沉法提取银杏外种皮中银杏酸的工艺研究 碱提酸沉法提取银杏外种皮中银杏酸的工艺研究
蚌埠医学院学报 Pub Date : 2019-09-30 DOI: 10.13898/j.cnki.issn.1000-2200.2019.09.034
马慧 | 李博涵 | 朱美林 | 李红梅 | 戴轶群 | 陈刚胜 | 吴成柱
{"title":"碱提酸沉法提取银杏外种皮中银杏酸的工艺研究","authors":"马慧 | 李博涵 | 朱美林 | 李红梅 | 戴轶群 | 陈刚胜 | 吴成柱","doi":"10.13898/j.cnki.issn.1000-2200.2019.09.034","DOIUrl":"https://doi.org/10.13898/j.cnki.issn.1000-2200.2019.09.034","url":null,"abstract":"","PeriodicalId":15256,"journal":{"name":"蚌埠医学院学报","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142052262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
正交试验比较金银花中绿原酸、异绿原酸A的提取工艺 正交试验比较金银花中绿原酸、异绿原酸A的提取工艺
蚌埠医学院学报 Pub Date : 2019-01-15 DOI: 10.13898/j.cnki.issn.1000-2200.2019.01.026
汤洁 | 程庆兵 | 付恩桃 | 刘修树 | 范高福 | 方丽波
{"title":"正交试验比较金银花中绿原酸、异绿原酸A的提取工艺","authors":"汤洁 | 程庆兵 | 付恩桃 | 刘修树 | 范高福 | 方丽波","doi":"10.13898/j.cnki.issn.1000-2200.2019.01.026","DOIUrl":"https://doi.org/10.13898/j.cnki.issn.1000-2200.2019.01.026","url":null,"abstract":"","PeriodicalId":15256,"journal":{"name":"蚌埠医学院学报","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142051524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
miRNA-21-5p通过pdcd4靶向调控胃癌细胞对顺铂敏感性的机制研究 miRNA-21-5p通过pdcd4靶向调控胃癌细胞对顺铂敏感性的机制研究
蚌埠医学院学报 Pub Date : 2018-01-01 DOI: 10.13898/j.cnki.issn.1000-2200.2018.10.017
王子安, 章海斌, 姚露岩, 刘玉, Chen Chu Ying
{"title":"miRNA-21-5p通过pdcd4靶向调控胃癌细胞对顺铂敏感性的机制研究","authors":"王子安, 章海斌, 姚露岩, 刘玉, Chen Chu Ying","doi":"10.13898/j.cnki.issn.1000-2200.2018.10.017","DOIUrl":"https://doi.org/10.13898/j.cnki.issn.1000-2200.2018.10.017","url":null,"abstract":"目的：探究微小RNA-21-5p（miRNA-21-5p）通过下调pdcd4基因表达调控胃癌耐药细胞系SGC-7901/DDP对顺铂敏感性机制的研究。方法：采用四甲基偶氮唑蓝（MTT）法检测不同浓度的顺铂（0、1、2、4、8、16、32 μmol/L）对胃癌细胞系SGC7901及胃癌耐药细胞系SGC7901/DDP增殖抑制的影响。通过实时荧光定量PCR检测SGC7901/DDP细胞中瞬时转染miRNA-21-5p inhibitors后miRNA-21-5p相对表达量的变化。采用Western blot及实时荧光定量PCR检测转染组、无关序列转染组（NC组）及空白转染组（CON组）分别转染miRNA-21-5p inhibitors及无关序列后对靶基因pdcd4蛋白及mRNA表达量的影响。结果：MTT实验结果显示耐药细胞SGC7901/DDP相对于亲本细胞SGC7901对顺铂不敏感（P<0.05）。实时荧光定量PCR结果表明，转染组miRNA-21-5p表达量均明显低于NC组和CON组（P<0.01），转染组pdcd4 mRNA表达量均明显高于NC组和CON组（P<0.01）。Western blot检测结果示，转染组pdcd4蛋白表达量均明显高于NC组和CON组（P<0.01）。结论：miRNA-21-5p表达水平的升高在一定程度上有利于胃癌细胞增殖，并通过下调pdcd4的表达，降低胃癌细胞对顺铂的敏感性。","PeriodicalId":15256,"journal":{"name":"蚌埠医学院学报","volume":"43 10","pages":"1339-1342"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147917397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
索拉非尼联合5-氮杂-2＇-脱氧胞苷对肝癌SMMC-7721细胞DNMT3B基因表达的影响 索拉非尼联合5-氮杂-2＇-脱氧胞苷对肝癌SMMC-7721细胞DNMT3B基因表达的影响
蚌埠医学院学报 Pub Date : 2017-01-01 DOI: 10.13898/j.cnki.issn.1000-2200.2017.03.002
韩正阳, 崔兵, 刘纪君, 贺晓珊, 梅传忠
{"title":"索拉非尼联合5-氮杂-2＇-脱氧胞苷对肝癌SMMC-7721细胞DNMT3B基因表达的影响","authors":"韩正阳, 崔兵, 刘纪君, 贺晓珊, 梅传忠","doi":"10.13898/j.cnki.issn.1000-2200.2017.03.002","DOIUrl":"https://doi.org/10.13898/j.cnki.issn.1000-2200.2017.03.002","url":null,"abstract":"目的：探讨索拉非尼联合5-氮杂-2'-脱氧胞苷（5-Aza-CdR）对人肝癌细胞SMMC-7721的作用及其调控DNMT3B基因表达可能的分子机制。方法：单独及联合给药后以MTT法测定SMMC-7721的增殖，Transwell检测其侵袭，流式细胞仪检测细胞凋亡，Western blot法观察DNMT3B、p-Akt-Ser473及ERK蛋白的表达。结果：索拉非尼、5-Aza-CdR对肝癌SMMC-7721细胞半数抑制浓度（IC50）分别为11、6 μmol/L，联合用药选取（6+4）μmol/L作为后续用药浓度。与对照组比较单药及联合用药均能抑制细胞侵袭、促进细胞凋亡、减少p-Akt-Ser473和DNMT3B蛋白的表达（P<0.05~P<0.01）。结论：索拉非尼和5-Aza-CdR联合用药能有效降低索拉非尼用药浓度，降低肝癌SMMC-7721细胞DNMT3B蛋白表达水平。","PeriodicalId":15256,"journal":{"name":"蚌埠医学院学报","volume":"42 3","pages":"285-289"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147887414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
远端预处理对体外循环下心脏瓣膜置换术患者脑损伤的保护作用 远端预处理对体外循环下心脏瓣膜置换术患者脑损伤的保护作用
蚌埠医学院学报 Pub Date : 2016-01-15 DOI: 10.13898/j.cnki.issn.1000-2200.2016.01.007
翁立军 | 张野 | 胡宪文 | 李云
{"title":"远端预处理对体外循环下心脏瓣膜置换术患者脑损伤的保护作用","authors":"翁立军 | 张野 | 胡宪文 | 李云","doi":"10.13898/j.cnki.issn.1000-2200.2016.01.007","DOIUrl":"https://doi.org/10.13898/j.cnki.issn.1000-2200.2016.01.007","url":null,"abstract":"","PeriodicalId":15256,"journal":{"name":"蚌埠医学院学报","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142042279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
补骨脂抗氧化及抗肿瘤活性成分的研究 补骨脂抗氧化及抗肿瘤活性成分的研究
蚌埠医学院学报 Pub Date : 2015-11-15 DOI: 10.13898/j.cnki.issn.1000-2200.2015.11.001
聂丽娟 | 李红梅 | 郭星 | 吴成柱 | 张玉心 | 霍强 | 马涛
{"title":"补骨脂抗氧化及抗肿瘤活性成分的研究","authors":"聂丽娟 | 李红梅 | 郭星 | 吴成柱 | 张玉心 | 霍强 | 马涛","doi":"10.13898/j.cnki.issn.1000-2200.2015.11.001","DOIUrl":"https://doi.org/10.13898/j.cnki.issn.1000-2200.2015.11.001","url":null,"abstract":"","PeriodicalId":15256,"journal":{"name":"蚌埠医学院学报","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142042191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
野生型及c.454C>T突变型人血管内皮生长因子A基因重组真核表达载体的体外表达 野生型及c.454C>T突变型人血管内皮生长因子A基因重组真核表达载体的体外表达
蚌埠医学院学报 Pub Date : 2015-01-01 DOI: 10.13898/j.cnki.issn.1000-2200.2015.02.003
Wu Yong, 刘学刚, 赵武, 徐家丽, 郭嘉诚, 李慧敏, 丁周志, 王亚明, 宋伟
{"title":"野生型及c.454C>T突变型人血管内皮生长因子A基因重组真核表达载体的体外表达","authors":"Wu Yong, 刘学刚, 赵武, 徐家丽, 郭嘉诚, 李慧敏, 丁周志, 王亚明, 宋伟","doi":"10.13898/j.cnki.issn.1000-2200.2015.02.003","DOIUrl":"https://doi.org/10.13898/j.cnki.issn.1000-2200.2015.02.003","url":null,"abstract":"目的：探讨野生型和c.454C〉T突变型人血管内皮生长因子A（VEGFA）基因重组真核表达载体（p EGFP-VEGFA）体外表达有无差异。方法：用脂质体法将野生型和突变型p EGFP-VEGFA转染人胚胎肾细胞（HEK293T）,采用荧光显微镜观察重组载体VEGFA和增强型绿色荧光蛋白（EGFP）的融合蛋白的表达,以实时荧光定量PCR法检测VEGFA mRNA的表达。结果：在经转染的HEK293T细胞内观察到绿色荧光,转染后48 h突变型p EGFP-VEGFA转染组荧光强度低于野生型p EGFPVEGFA转染组。突变型p EGFP-VEGFA转染组VEGFA mRNA表达明显低于野生型p EGFP-VEGFA转染组（P〈0.01）。结论：在HEK293T细胞中,初步表明VEGFA突变体导致VEGFA mRNA和蛋白的表达水平均下调,为进一步研究VEGFA c.454C〉T突变体在先天性左心室流出道病变发生中的作用奠定了基础。","PeriodicalId":15256,"journal":{"name":"蚌埠医学院学报","volume":"40 2","pages":"148-151"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147885840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
星点设计-效应面法优化及制备安妥沙星眼用温度敏感原位凝胶 星点设计-效应面法优化及制备安妥沙星眼用温度敏感原位凝胶
蚌埠医学院学报 Pub Date : 2013-07-15 DOI: 10.13898/j.cnki.issn.1000-2200.2013.07.001
孙思雨 | 马涛 | 王清清
{"title":"星点设计-效应面法优化及制备安妥沙星眼用温度敏感原位凝胶","authors":"孙思雨 | 马涛 | 王清清","doi":"10.13898/j.cnki.issn.1000-2200.2013.07.001","DOIUrl":"https://doi.org/10.13898/j.cnki.issn.1000-2200.2013.07.001","url":null,"abstract":"","PeriodicalId":15256,"journal":{"name":"蚌埠医学院学报","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142038963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信
小红书