Journal of Applied Pharmacy最新文献_第10页

Clinical Pharmaceutical Care, Medical Laboratory Imaging, NuclearMedicine: A Synergy to Improve Clinical Outcomes and Reducing Costs 临床药学护理，医学实验室成像，核医学:提高临床结果和降低成本的协同作用

Journal of Applied Pharmacy Pub Date : 2016-06-21 DOI: 10.21065/1920-4159.1000E112

M. Luisetto

引用次数: 4

Darwinian Principles toward Multidrug-Resistant Cancer Cells 多药耐药癌细胞的达尔文原理

Journal of Applied Pharmacy Pub Date : 2016-06-21 DOI: 10.21065/1920-4159.1000E111

M. Varol

引用次数: 2

Coexpression of Genetically Engineered Cyt b5-CYP3A4 Fusion Protein withPOR in Sf9 Insect Cells and Functional Characterization of the ExpressedProducts in vitro 基因工程Cyt b5-CYP3A4融合蛋白与por在Sf9昆虫细胞中的共表达及表达产物的体外功能表征

Journal of Applied Pharmacy Pub Date : 2016-06-03 DOI: 10.21065/1920-4159.1000223

Zhangming Xie, Shabbir Ahmed, Wenhui Liu, Si-si Kong, Yingchun Xu, Ting Liu, Shuqing Chen

{"title":"Coexpression of Genetically Engineered Cyt b5-CYP3A4 Fusion Protein withPOR in Sf9 Insect Cells and Functional Characterization of the ExpressedProducts in vitro","authors":"Zhangming Xie, Shabbir Ahmed, Wenhui Liu, Si-si Kong, Yingchun Xu, Ting Liu, Shuqing Chen","doi":"10.21065/1920-4159.1000223","DOIUrl":"https://doi.org/10.21065/1920-4159.1000223","url":null,"abstract":"Human cytochrome P450 3A4 (CYP3A4) is the most abundant phase I drug-metabolizing enzyme in the liver, and approximately 50% of drugs on the market are metabolized by CYP3A4. Therefore, many in vitro studies relied on recombinant CYP3A4 as screening tool to evaluate potential drug-drug interactions (DDIs) in vivo. However, limited information regarding recombinant CYP3A4 with high catalytic activity is available. So, the present study aimed to obtain recombinant CYP3A4 with high catalytic activity and to characterize its functions in vitro. To enhance the catalytic activities of heterologously expressed CYP3A4, the enzyme was fused to cytochrome b5 (b5) tail-to-head, and the fused enzyme was inserted together with NADPH–P450 reductase (POR) into a single plasmid to achieve a simultaneous expression in sf9 cells. Here, substrate binding affinities, enzymatic activities and applications in in vitro DDIs of the fused enzyme were investigated. The dissociation constant Kd of POR-cyt b5CYP3A4 was 8.3 ± 0.87 μmol/L, the Clint (Clint=Vmax/Km) was 8.57 mL/min/g protein for POR-cyt b5CYP3A4 in the metabolism of testosterone and 150.3 mL/min/g protein for midazolam. In addition, the inhibitory constant Ki of ketoconazole on testosterone metabolism was 0.013 ± 0.0038 μmol/L. The present results suggested significantly increased substrate binding affinity and enzymatic activity for the fused enzyme. Thus, the construct could be helpful for studying drug metabolisms and DDIs investigation associated with CYP3A4 in vitro. In addition, simultaneous expression of the fused enzyme and POR could provide more reproducible results based on a more stable molar ratio of CYP3A4/POR/b5.","PeriodicalId":15238,"journal":{"name":"Journal of Applied Pharmacy","volume":"98 2 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2016-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79461682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A Short Review on Chiral Alcohols Verses Bio-Catalysis 手性醇与生物催化研究综述

Journal of Applied Pharmacy Pub Date : 2016-05-28 DOI: 10.21065/1920-4159.1000222

S. Chittamuru, B. Reddy, A. Rao

{"title":"A Short Review on Chiral Alcohols Verses Bio-Catalysis","authors":"S. Chittamuru, B. Reddy, A. Rao","doi":"10.21065/1920-4159.1000222","DOIUrl":"https://doi.org/10.21065/1920-4159.1000222","url":null,"abstract":"Catalyst is a chemical molecule or metal substance which enhances the rate of reaction is called catalyst, the catalyst present in the living organisms to carryout biochemical reactions or metabolic pathways. Exames oxidoreductases, lyases, ligages, proteases, hydrolases, esterases, pectinases etc. are Different methodologies available in synthesis of chiral molecules chemical/biocatalysis. The enormous potential of biocatalysts (microorganisms and enzymes) in synthesis of chiral molecules in mild conditions (pH and temperate) with high chemo-, regio-, enantio and functional selectivity with decrease formation of by-products, with short reaction steps (reactions which are not easily conducted classical organic reactions) (Long chemical processes with tedious blocking and de-blocking steps). Thus the use of biocatalyst has attracted a great attention from the green chemistry perspective (expensive chiral reagents/ environmentally hazardous heavy metals). Biocatalysts are well known for their enzymatic activity towards numerous reactions ranging from in-vivo living cells (biochemical pathways) to in-vitro chemical reactions (reduction and transesterification) with enantiomeric purity and specificity. In this review the discussion is about synthesis of chiral using different kinds of biocatalysts. Daucus Carota, Pisum Sativa, Novazyme P-435, as biocatalysts for chiral alcohols.","PeriodicalId":15238,"journal":{"name":"Journal of Applied Pharmacy","volume":"79 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2016-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75359266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

New Multi-Particle Systems for Colon-Targeted Meloxicam 结肠癌靶向美洛昔康的新型多粒子系统

Journal of Applied Pharmacy Pub Date : 2016-05-19 DOI: 10.21065/1920-4159.1000221

E. Ruiz, Covadonga Álvarez, J. Rodriguez, S. Durán, S. Durán, Dellias Pm

{"title":"New Multi-Particle Systems for Colon-Targeted Meloxicam","authors":"E. Ruiz, Covadonga Álvarez, J. Rodriguez, S. Durán, S. Durán, Dellias Pm","doi":"10.21065/1920-4159.1000221","DOIUrl":"https://doi.org/10.21065/1920-4159.1000221","url":null,"abstract":"Meloxicam (MLX) is a non-steroidal anti-inflammatory drug (NSAIDs) from the Oxicam family. This group of NSAIDs has been highly used in the treatment of rheumatoid arthritis and post-operative inflammation and is known as good antioxidants. Recently, their activity in chemoprevention, chemo-suppression, UV-sensitization and UVprotection was also identified. MLX has been described as a COX-2 selective inhibitor. Its use has some advantages regarding to its selectivity, namely, less adverse effects as gastrointestinal aggression and anticlotting activity. As MLX is better absorbed in colon and its properties against colon cancer and colonic inflammatory diseases are being studied, it is interesting to investigate a new MLX formulation for colonic delivery. We are studying the solubility and the dissolution of different combined formulations at pH 1.2, 6.8 and 7.4 to mimic their absorbance in the colon. These formulations are composed by different excipients that provide pH and time-dependent deliveries such as cellulose (Metolose®) and methacrylic acid esters with quaternary ammonium groups (EUDRAGIT® RS 30D, EUDRAGIT® FS 30D and EUDRAGIT® NM 30D).","PeriodicalId":15238,"journal":{"name":"Journal of Applied Pharmacy","volume":"43 1 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2016-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79965435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Utilization Assessment of Surgical Antibiotic Prophylaxis at Ayder Referral Hospital, Northern Ethiopia 埃塞俄比亚北部Ayder转诊医院外科抗生素预防使用评估

Journal of Applied Pharmacy Pub Date : 2016-04-27 DOI: 10.4172/1920-4159.1000220

S. A. Mohamoud, Teshager Aklilu Yesuf, Eskinder Ayalew Sisay

{"title":"Utilization Assessment of Surgical Antibiotic Prophylaxis at Ayder Referral Hospital, Northern Ethiopia","authors":"S. A. Mohamoud, Teshager Aklilu Yesuf, Eskinder Ayalew Sisay","doi":"10.4172/1920-4159.1000220","DOIUrl":"https://doi.org/10.4172/1920-4159.1000220","url":null,"abstract":"Introduction: Surgical site infection represents a significant burden in terms of patients’ morbidity, mortality and hospital costs which can be prevented using prophylaxis. \u0000 \u0000Objective: To assess rate of compliance to Surgical Antibiotic Prophylaxis (SAP) guidelines at Ayder Referral Hospital (ARH). \u0000 \u0000Method: Prospective cross-sectional study was conducted from 12th March to 28thApril, 2015. Data were collected using data abstraction checklist for all patients who underwent surgery and met inclusion criteria. SAP Guidelines and CDC Wound Classification were used as data assessment protocols. Epidata 3.1 and SPSS 16 were used for data entry and analysis of descriptive statistics. \u0000 \u0000Results: A total of 196 patients with mean age of 37.84 years were recruited (female, 58.7%). Of these, 62.2% received SAP but prophylaxis was needed in 58.2%. The total compliance to SAP guideline was 21.9% and 25% for national Standard Treatment Guideline (STG) and American Society of Health-system Pharmacist (ASHP) guideline respectively. Selection of SAP (national STG 100% versus ASHP Guideline 89.5%) was the most deviated parameter from SAP guidelines followed by duration (63.5%), indication (19.4%) and dose (10.4%). Most commonly used agent was ceftriaxone (85.2%). \u0000 \u0000Conclusion: Current practice of ARH is hugely divergent from SAP guidelines. Use of broader spectrum antibiotics for an extended period was common.","PeriodicalId":15238,"journal":{"name":"Journal of Applied Pharmacy","volume":"42 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2016-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87011297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Synthesis Characterization and Biological Influence of Some NewlySynthesized Mix ligand Chelates 一些新合成的混合配体螯合物的合成、表征及其生物学影响

Journal of Applied Pharmacy Pub Date : 2016-04-14 DOI: 10.4172/1920-4159.1000219

ya Dr

引用次数: 0

Nanopeptides: Non-Covalent Interactions in Chemistry and Biological Functions 纳米肽:化学和生物功能中的非共价相互作用

Journal of Applied Pharmacy Pub Date : 2016-04-07 DOI: 10.4172/1920-4159.1000218

C. Selvakkumar, K. Muthusamy, Sathishkumar Chinnasamy

{"title":"Nanopeptides: Non-Covalent Interactions in Chemistry and Biological Functions","authors":"C. Selvakkumar, K. Muthusamy, Sathishkumar Chinnasamy","doi":"10.4172/1920-4159.1000218","DOIUrl":"https://doi.org/10.4172/1920-4159.1000218","url":null,"abstract":"The interactions involving the side chains of weakly polar aromatic amino acid residues, e.g., Phenylalanine (Phe), Tyrosine (Tyr) and Tryptophan (Trp) generally reside at the interior of proteins and help in the stabilization of globular protein structures. The aromatic electron cloud of the aromatic rings of these amino acids are delocalized on both sides of the planer rings, so that there is a small partial negative charge on the face and a small partial positive charge on the hydrogen atoms of the edge, which leads to the possibility of electrostatic interactions. These interaction play a vital role nanofiber based vaccine adjuvants, and cocaine vaccine development and increasing interest and structure based drug development. Apart from electrostatic forces, aromatic interactions also consist of van der Waals and hydrophobic forces. These weakly polar interactions are enthalpically comparable to a hydrogen bond. Protein engineering methods have revealed that introducing aromatic pairs and aromatic clusters increases the thermal stability of proteins and it has been demonstrated that the introduction of an additional aromatic interaction improved the thermophilicity and thermostability of the family of 11 xylanase. These weakly polar interactions also have a significant role in the stability of DNA. Different types of weakly polar interactions involving the aromatic side chains are discussed below.","PeriodicalId":15238,"journal":{"name":"Journal of Applied Pharmacy","volume":"33 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91380530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Design, Synthesis and Development of Stereo Chemical Constraints into β -Amino Acid Residues: Gabapentin Structural Data Role in Nerve Pain Medication β -氨基酸残基立体化学约束的设计、合成和发展:加巴喷丁在神经痛药物中的结构数据作用

Journal of Applied Pharmacy Pub Date : 2016-04-06 DOI: 10.4172/1920-4159.1000217

Saravana Kumar Kailasam Mani, R. Narayanasamy

引用次数: 2

Conformational Angles and Properties of andOmega;-Amino Acids in ProteinFolding 蛋白质折叠中和-氨基酸的构象角和性质

Journal of Applied Pharmacy Pub Date : 2016-04-06 DOI: 10.4172/1920-4159.1000216

VS Saravana Mani, R. Narayanasamy

引用次数: 0