Journal of Cellular Physiology最新文献_第8页

Shear stress effects on epididymal epithelial cell via primary cilia mechanosensory signaling 剪切应力通过初级纤毛机械感觉信号对附睾上皮细胞产生影响

IF 4.5 2区生物学

Journal of Cellular Physiology Pub Date : 2024-11-07 DOI: 10.1002/jcp.31475

Sepideh Fakhari, Gabriel Campolina-Silva, Farnaz Asayesh, Laura Girardet, Marie-Pier Scott-Boyer, Arnaud Droit, Denis Soulet, Jesse Greener, Clémence Belleannée

{"title":"Shear stress effects on epididymal epithelial cell via primary cilia mechanosensory signaling","authors":"Sepideh Fakhari, Gabriel Campolina-Silva, Farnaz Asayesh, Laura Girardet, Marie-Pier Scott-Boyer, Arnaud Droit, Denis Soulet, Jesse Greener, Clémence Belleannée","doi":"10.1002/jcp.31475","DOIUrl":"10.1002/jcp.31475","url":null,"abstract":"Shear stress, resulting from fluid flow, is a fundamental mechanical stimulus affecting various cellular functions. The epididymis, essential for sperm maturation, offers a compelling model to study the effects of shear stress on cellular behavior. This organ undergoes extensive proliferation and differentiation until puberty, achieving full functionality as spermatozoa commence their post-testicular maturation. Although the mechanical tension exerted by testicular fluid is hypothesized to drive epithelial proliferation and differentiation, the precise mechanisms remain unclear. Here we assessed whether the responsiveness of the epididymal cells to shear stress depends on functional primary cilia by combining microfluidic strategies on immortalized epididymal cells, calcium signaling assays, and high-throughput gene expression analysis. We identified 97 genes overexpressed in response to shear stress, including early growth response (Egr) 2/3, cellular communication network factor (Ccn) 1/2, and Fos proto-oncogene (Fos). While shear stress triggered a rapid increase of intracellular Ca2+, this response was abrogated following the impairment of primary ciliogenesis through pharmacological and siRNA approaches. Overall, our findings provide valuable insights into how mechanical forces influence the development of the male reproductive system, a requisite to sperm maturation.","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"240 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11733861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NAT10 functions as a pivotal regulator in gastric cancer metastasis and tumor immunity NAT10 是胃癌转移和肿瘤免疫的关键调节因子。

IF 4.5 2区生物学

Journal of Cellular Physiology Pub Date : 2024-10-28 DOI: 10.1002/jcp.31474

Yuqian Mo, Enyu Huang, Chao Deng, Haofeng Huang, Ying Zhu, Xinlong Wei, Jinlin Zhong, Yuzhi Wang, Zhigang Huang, Jingjing Zhang

{"title":"NAT10 functions as a pivotal regulator in gastric cancer metastasis and tumor immunity","authors":"Yuqian Mo, Enyu Huang, Chao Deng, Haofeng Huang, Ying Zhu, Xinlong Wei, Jinlin Zhong, Yuzhi Wang, Zhigang Huang, Jingjing Zhang","doi":"10.1002/jcp.31474","DOIUrl":"10.1002/jcp.31474","url":null,"abstract":"Gastric cancer (GC) presents a significant global health burden, with metastasis being the leading cause of treatment failure and mortality. NAT10, a regulatory protein involved in mRNA acetylation, has been implicated in various cancers. However, its role in GC, especially concerning metastasis and immune interactions, remains unclear. Utilizing multi-omics data from gastric cancer samples, we conducted comprehensive analyses to investigate NAT10 expression, its correlation with clinical parameters and immune relevance. Bioinformatics analysis and digital image processing were employed for this purpose. Furthermore, in vitro and in vivo experiments were conducted to elucidate the functional role of NAT10 in gastric cancer progression, aiming to provide deeper biological insights. Our findings reveal a significant association between NAT10 expression and various aspects of transcriptional, protein, as well as tumor immunity in GC patients. Additionally, we demonstrated that NAT10 promotes gastric cancer cell proliferation and migration, both in cellular models and in animal studies, suggesting its involvement in early tumor microvascular metastasis. NAT10 emerges as a promising molecular target, offering potential avenues for further research into molecular mechanisms and therapeutic strategies for GC.","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"240 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic regulation of myogenesis by vitamin C 维生素 C 对肌肉生成的表观遗传调控

IF 4.5 2区生物学

Journal of Cellular Physiology Pub Date : 2024-10-24 DOI: 10.1002/jcp.31472

Sachiko Yamashita Takeuchi, Chirada Dusadeemeelap, Tatsuo Kawamoto, Takuma Matsubara, Shoichiro Kokabu, William N. Addison

{"title":"Epigenetic regulation of myogenesis by vitamin C","authors":"Sachiko Yamashita Takeuchi, Chirada Dusadeemeelap, Tatsuo Kawamoto, Takuma Matsubara, Shoichiro Kokabu, William N. Addison","doi":"10.1002/jcp.31472","DOIUrl":"10.1002/jcp.31472","url":null,"abstract":"The micronutrient vitamin C is essential for the maintenance of skeletal muscle health and homeostasis. The pro-myogenic effects of vitamin C have long been attributed to its role as a general antioxidant agent, as well as its role in collagen matrix synthesis and carnitine biosynthesis. Here, we show that vitamin C also functions as an epigenetic compound, facilitating chromatin landscape transitions during myogenesis through its activity as an enzymatic cofactor for histone H3 and DNA demethylation. Utilizing C2C12 myoblast cells to investigate the epigenetic effects of vitamin C on myogenesis, we observe that treatment of cells with vitamin C decreases global H3K9 methylation and increases 5-hmC levels. Furthermore, vitamin C treatment enhances myoblast marker gene expression and myotube formation during differentiation. We identify KDM7A as a histone lysine demethylase markedly upregulated during myogenesis. Accordingly, knockdown of Kdm7a prevents the pro-myogenic effects of vitamin C. Chromatin immunoprecipitation analysis showed that KDM7A occupies the promoter region of the myogenic transcription factor MyoD1 where it facilitates histone demethylation. We also confirm that the methylcytosine dioxygenases TET1 and TET2 are required for myogenic differentiation and that their loss blunts stimulation of myogenesis by vitamin C. In conclusion, our data suggest that an epigenetic mode of action plays a major role in the myogenic effects of vitamin C.","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"240 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fish collagen sponge with human umbilical cord mesenchymal stem cells for diabetic wound repair in rats 鱼胶原海绵与人脐带间充质干细胞用于大鼠糖尿病伤口修复。

IF 4.5 2区生物学

Journal of Cellular Physiology Pub Date : 2024-10-21 DOI: 10.1002/jcp.31471

Che Haijie, Wang Lei, Wang Kai, Lin Guodong, Liu Guolong, Yang Zhongzhen, Wang Junru, Liu Ying, Jiang Xiaorui

{"title":"Fish collagen sponge with human umbilical cord mesenchymal stem cells for diabetic wound repair in rats","authors":"Che Haijie, Wang Lei, Wang Kai, Lin Guodong, Liu Guolong, Yang Zhongzhen, Wang Junru, Liu Ying, Jiang Xiaorui","doi":"10.1002/jcp.31471","DOIUrl":"10.1002/jcp.31471","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 Stem cell therapy offers a novel approach to treating difbetic foot ulcers. Fish skin decellularized matrix, a type I collagen, provides a promising carrier for stem cells, creating a supportive microenvironment that enhances cell survival and therapeutic potential. This study aims to investigate the effects and mechanisms of human umbilical cord mesenchymal stem cells (HUCMSCs) loaded onto a fish collagen sponge for wound healing in diabetic rats. The study evaluates stem cell-loading efficiency with fish collagen sponge in vitro, assesses material distribution on diabetic rat wounds, and establishes a wound model. Rats are divided into the Self-healing group, Fish collagen sponge group, and Sponge loaded with HUCMSCs group. Therapeutic effects are evaluated through various analyses, including histopathology and reverse transcription polymerase chain reaction for collagen-related gene expression levels. Compared to the self-healing group, both the fish collagen group and the composite group show faster wound repair and improved healing outcomes. The composite group exhibits superior wound healing quality, with fish collagen contributing to enhanced tissue regeneration through collagen regulation at the wound site. Loading HUCMSCs onto a fish collagen sponge shows promise for treating diabetic wounds by addressing nutrient deficiency and cell supply issues, offering potential benefits for patients undergoing treatment.\u0000 </section>\u0000 </div>","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"240 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RHO subfamily of small GTPases in the development and function of hematopoietic cells 小 GTP 酶 RHO 亚家族在造血细胞发育和功能中的作用。

IF 4.5 2区生物学

Journal of Cellular Physiology Pub Date : 2024-10-21 DOI: 10.1002/jcp.31469

Stephany Suelen de Castro Sampaio, Maria Carolina Clares Ramalho, Caroline Santos de Souza, Beatriz de Almeida Rodrigues, Guilherme Ramos Sales de Mendonça, Mariana Lazarini

引用次数: 0

HO-1: An emerging target in fibrosis HO-1：纤维化的新目标。

IF 4.5 2区生物学

Journal of Cellular Physiology Pub Date : 2024-10-17 DOI: 10.1002/jcp.31465

Chenxi Lu, Yuan Liu, Feifei Ren, Haoran Zhang, Yafang Hou, Hong Zhang, Zhiyong Chen, Xia Du

{"title":"HO-1: An emerging target in fibrosis","authors":"Chenxi Lu, Yuan Liu, Feifei Ren, Haoran Zhang, Yafang Hou, Hong Zhang, Zhiyong Chen, Xia Du","doi":"10.1002/jcp.31465","DOIUrl":"10.1002/jcp.31465","url":null,"abstract":"Fibrosis, an aberrant reparative response to tissue injury, involves a disruption in the equilibrium between the synthesis and degradation of the extracellular matrix, leading to its excessive accumulation within normal tissues, and culminating in organ dysfunction. Manifesting in the terminal stages of nearly all chronic ailments, fibrosis carries a high mortality rate and poses a significant threat to human health. Heme oxygenase-1 (HO-1) emerges as an endogenous protective agent, mitigating tissue damage through its antioxidant, anti-inflammatory, and antiapoptotic properties. Numerous studies have corroborated HO-1's potential as a therapeutic target in anti-fibrosis treatment. This review delves into the structural and functional attributes, and the upstream and downstream pathways of HO-1. Additionally, the regulatory networks and mechanisms of HO-1 in cells associated with fibrosis are elucidated. The role of HO-1 in various fibrosis-related diseases is also explored. Collectively, this comprehensive information serves as a foundation for future research and augments the viability of HO-1 as a therapeutic target for fibrosis.","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"240 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacological activity, phytochemistry, and organ protection of lithospermic acid 石杉碱甲的药理活性、植物化学和器官保护。

IF 4.5 2区生物学

Journal of Cellular Physiology Pub Date : 2024-10-14 DOI: 10.1002/jcp.31460

Wenwen Yang, Jiayan Li, Jiayin Tian, Xiaoyi Liu, Wentao Xie, Xue Wu, Zhe Zhang, Yuefei Song, Shuya Wang, Shiyan Zhao, Zheng Wang, Yang Yang, Zhenxiao Jin

{"title":"Pharmacological activity, phytochemistry, and organ protection of lithospermic acid","authors":"Wenwen Yang, Jiayan Li, Jiayin Tian, Xiaoyi Liu, Wentao Xie, Xue Wu, Zhe Zhang, Yuefei Song, Shuya Wang, Shiyan Zhao, Zheng Wang, Yang Yang, Zhenxiao Jin","doi":"10.1002/jcp.31460","DOIUrl":"10.1002/jcp.31460","url":null,"abstract":"Lithospermic acid (LA) is a water-soluble phenolic acid compound extracted and separated from the dried root and the rhizome of Salviamiltiorrhiza Bge (Labiatae), possessing multiple biological activities. Firstly, in terms of pharmacological activities, LA has been proven to possess anti-inflammatory, antioxidant, autophagy activation, and antiapoptotic properties. Secondly, the pharmacokinetic characteristics of LA show rapid and extensive distribution in various tissues after intravenous administration, followed by rapid elimination and excretion. Additionally, potential therapeutic effects of LA have been found in various diseases such as thrombosis, Parkinson's disease, hepatitis B, diabetes, and psoriasis, among others. Particularly, LA has shown promising prospects in the treatment of clinical heart diseases and has been included in new drug formulations for the treatment of chronic angina, demonstrating superior efficacy compared to current cardiovascular drugs. In conclusion, this review comprehensively introduces the pharmacological mechanisms, pharmacokinetics, and protective effects in diseases of LA. These information can lay a theoretical foundation for the future development and new clinical applications of LA.","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"240 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leukotriene B4 is elevated in diabetes and promotes ventricular arrhythmogenesis in guinea pig 白三烯 B4 在糖尿病患者体内升高，并促进豚鼠室性心律失常的发生。

IF 4.5 2区生物学

Journal of Cellular Physiology Pub Date : 2024-10-14 DOI: 10.1002/jcp.31467

Andrea Corbin, Kelly A. Aromolaran, Ademuyiwa S. Aromolaran

{"title":"Leukotriene B4 is elevated in diabetes and promotes ventricular arrhythmogenesis in guinea pig","authors":"Andrea Corbin, Kelly A. Aromolaran, Ademuyiwa S. Aromolaran","doi":"10.1002/jcp.31467","DOIUrl":"10.1002/jcp.31467","url":null,"abstract":"Diabetes (DM) patients have an increased risk (~50%) for sudden cardiac death (SCD), mostly as a result of ventricular arrhythmias. The molecular mechanisms involved remain partially defined. The potent proinflammatory lipid mediator leukotriene (LT) B4, is pathologically elevated in DM compared to nondiabetic patients, resulting in increased LTB4 accumulation in heart, leading to an increased risk for life-threatening proarrhythmic signatures. We used electrophysiology, immunofluorescence, and confocal microscopy approaches to evaluate LTB4 cellular effects in guinea pig heart and ventricular myocytes. We have observed that LTB4 is increased in multiple mouse models (C57BL/6 J/Lepob/ob and PANIC-ATTAC) of DM, promotes profound cellular arrhythmogenesis (spontaneous beats and early after depolarizations, EADs), and severely depresses the rapidly activating delayed rectifier K current (hERG1/IKr) density in HEK293 cells and guinea pig ventricular myocytes. We have further found that guinea pigs challenged with LTB4 displayed a significantly prolonged QT interval, and that this can be prevented with LTB4R inhibition, suggesting that preventing such LTB4R effects may be therapeutically beneficial in DM. Our data suggests that a further elucidation of LTB4 vulnerable substrates, and how this leads to ventricular arrhythmias, is likely to lead to continued improvements in management options, and the development of new therapies for prevention of SCD in DM patients.","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"240 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11733858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum: RA promotes proliferation of primordial germ cell-like cells differentiated from porcine skin-derived stem cells 更正：RA可促进由猪皮肤干细胞分化而来的原始生殖细胞样细胞的增殖。

IF 4.5 2区生物学

Journal of Cellular Physiology Pub Date : 2024-10-14 DOI: 10.1002/jcp.31470

引用次数: 0

N-terminomics profiling of naïve and inflamed murine colon reveals proteolytic signatures of legumain 新生和发炎小鼠结肠的 N 端组学分析揭示了豆豆蛋白酶的蛋白水解特征。

IF 4.5 2区生物学

Journal of Cellular Physiology Pub Date : 2024-10-11 DOI: 10.1002/jcp.31466

Alexander R. Ziegler, Bethany M. Anderson, Rocco Latorre, Rachel M. McQuade, Antoine Dufour, Brian L. Schmidt, Nigel W. Bunnett, Nichollas E. Scott, Laura E. Edgington-Mitchell

{"title":"N-terminomics profiling of naïve and inflamed murine colon reveals proteolytic signatures of legumain","authors":"Alexander R. Ziegler, Bethany M. Anderson, Rocco Latorre, Rachel M. McQuade, Antoine Dufour, Brian L. Schmidt, Nigel W. Bunnett, Nichollas E. Scott, Laura E. Edgington-Mitchell","doi":"10.1002/jcp.31466","DOIUrl":"10.1002/jcp.31466","url":null,"abstract":"Legumain is a cysteine protease broadly associated with inflammation. It has been reported to cleave and activate protease-activated receptor 2 to provoke pain associated with oral cancer. Outside of gastric and colon cancer, little has been reported on the roles of legumain within the gastrointestinal tract. Using a legumain-selective activity-based probe, LE28, we report that legumain is activated within colonocytes and macrophages of the murine colon, and that it is upregulated in models of acute experimental colitis. We demonstrated that loss of legumain activity in colonocytes, either through pharmacological inhibition or gene deletion, had no impact on epithelial permeability in vitro. Moreover, legumain inhibition or deletion had no obvious impacts on symptoms or histological features associated with dextran sulfate sodium-induced colitis, suggesting its proteolytic activity is dispensable for colitis initiation. To gain insight into potential functions of legumain within the colon, we performed field asymmetric waveform ion mobility spectrometry-facilitated quantitative proteomics and N-terminomics analyses on naïve and inflamed colon tissue from wild-type and legumain-deficient mice. We identified 16 altered cleavage sites with an asparaginyl endopeptidase signature that may be direct substrates of legumain and a further 16 cleavage sites that may be indirectly mediated by legumain. We also analyzed changes in protein abundance and proteolytic events broadly associated with colitis in the gut, which permitted comparison to recent analyses on mucosal biopsies from patients with inflammatory bowel disease. Collectively, these results shed light on potential functions of legumain and highlight its potential roles in the transition from inflammation to colorectal cancer.","PeriodicalId":15220,"journal":{"name":"Journal of Cellular Physiology","volume":"240 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142400415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0