Isaac Baani, Paul Amihere-Ackah, B. M. Tiimub, R. W. Tiimob, John Adoripore, Gideon Likida Tiimob
{"title":"Indigenous processing technologies from farm gates adulterate honey quality at local markets and subject consumers to critical health risks","authors":"Isaac Baani, Paul Amihere-Ackah, B. M. Tiimub, R. W. Tiimob, John Adoripore, Gideon Likida Tiimob","doi":"10.31248/JBBD2018.075","DOIUrl":"https://doi.org/10.31248/JBBD2018.075","url":null,"abstract":"Physico-chemical and microbial status of 400 honey samples picked from 200 experienced apiculturists for laboratory elucidation based on indigenous processing techniques (IPTs) comprising (burning (B); crushing and straining (CS mashing and straining (MS boiling and straining (BS C&S and M&S IPTs resulted to lower FA values (8.10 ± 0.015, 9.50 ± 0.015 and 4.20 ± 0.030) me/kg respectively compared to B&S (10.00 ± 1.00 - 36.67 ± 0.58) me/kg. Greater variation in FA levels confirms the speculations of experts on its fluctuation tendency with honey. Indigenous B gave rise to both highest TRS (31%) and TVMLs (6.95 ± 0.044 cfu/ml). The entire IPTs ubiquitously impacted health threats on honey consumers emanating from microbial contamination. The C&S and M&S IPTs are proved as best honey processing techniques for large scale application at farmgates in Ghana because it conducts the highest counteractive pH to microbial invasions. Innovations on these improved methods could reduce consumer health risks by 90 to 95%.","PeriodicalId":15121,"journal":{"name":"Journal of Bioscience and Biotechnology Discovery","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86252673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Uba, E. Okoye, O. M. Nworah, T. Azuanichie, C. Dokubo
{"title":"Biostimulatory effect of organic and inorganic nutrients on soil biological indicators in diesel contaminated soil","authors":"B. Uba, E. Okoye, O. M. Nworah, T. Azuanichie, C. Dokubo","doi":"10.31248/JBBD2018.087","DOIUrl":"https://doi.org/10.31248/JBBD2018.087","url":null,"abstract":"Conventional soil remediation has been known to be expensive treatment. Thus, various strategies have been opted to investigate cost-effective solution to contaminated sites and an example is bio-stimulation. The study investigated effect of organic and inorganic nutrients on soil biological indicators of diesel – contaminated soil. The parameters investigated include determination of pH, conductivity, nitrate, phosphate, total organic carbon, moisture content, temperature, baseline, soil respiration, dehydrogenase activity, urease activity, soil microbial biomass carbon, protease activity, germination index (GI), total viable count technique (THBC and TCHUB), microscopic and biochemical characteristics in 56th days experimental period. The result revealed that the soil is neutral to slightly alkaline in pH, moderate in conductivity, low in nitrate, phosphate and total organic carbon contents, high in moisture content with moderate (mesophilic) temperature. The result of baseline study also revealed that soil respiration is 99.30 s days, dehydrogenase activity is 02.69 µg TPFg-1 dm (24 hr)-1), microbial biomass carbon is 02.69 mg/kg, urease activity is 24.52 µmol N-NH4 / g 2 hrs, protease activity is 36.66 µg / ml, GI is 09.24 %, THBC is 06.53 logCFU /g and TCHUB 06.73 logCFU /g. There were significant effect (P 0.05) on soil respiration, soil microbial biomass carbon, protease activity and germination index respectively by the organic and inorganic nutrients. There was negative and positive correlations between germination index, microbial count and other biological indicators measured. More so, the result revealed that the hydrocarbon utilizing bacterial isolates were Aeromonas sp., Serratia fonticola, Citrobacter freundii, Citrobacter diversus and Corynebacterium sp. Thus, application of organic and inorganic nutrients sources is suggested as a good strategy for bioremediation of diesel impacted soil.","PeriodicalId":15121,"journal":{"name":"Journal of Bioscience and Biotechnology Discovery","volume":"23 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82962311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chemical composition, phytochemical and mineral profile of garlic (Allium sativum)","authors":"A. Yusuf, S. Fagbuaro, S. O. Fajemilehin","doi":"10.31248/JBBD2018.073","DOIUrl":"https://doi.org/10.31248/JBBD2018.073","url":null,"abstract":"The practice of complementary and alternative medicine is now on the increase in developing countries in response to World Health Organization’s directives. This has culminated in several pre-clinical and clinical studies that have provided the scientific basis for the efficacy of many plants used in folk medicine to improve growth performance in farm animals and treat infections. This study was carried out to investigate the chemical, mineral and phytochemical contents of garlic as a preliminary work to evaluate the influence of garlic on the performance characteristics of broiler chickens. Garlic bulbs (Allium sativum), including cloves and hulls, were obtained from the open market at Ado-Ekiti-Nigeria. The garlic bulbs were desegmented into cloves and were cut into chips. The chips were sun-dried for 3 weeks. The dried garlic chips were milled and analysed for the chemical compositions. The results obtained indicated that garlic on dry matter basis contained 4.55 mg/100g, 73.22 mg/100g and 15.33 mg/100g of moisture, carbohydrate and crude protein respectively. The crude fat was 0.72 mg/100g while crude fibre and ash were 2.10 mg/100g and 4.08 mg/100g, respectively. Garlic contained 10.19, 26.30, 10.19, 5.29, 0.001, 0.34 and 0.001 mg/100g of potassium, calcium, phosphorus, iron, magnesium, zinc and manganese respectively while lead and cobalt were below detection level. Garlic is acidic (pH = 3.91) and contained 4.21, 3.54, 0.64, 0.80, 5.56, 0.04 and 0.02 mg/100g of alkaloids, tannins, carotenoids, saponin, flavonoids, steroids and cardenolides, respectively. Garlic as a feed additive possesses nutritional properties for use in the enhancement of improved livestock production.","PeriodicalId":15121,"journal":{"name":"Journal of Bioscience and Biotechnology Discovery","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86935702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Laminu, M. Yakubu, H. Bashir, M. A. Milala, B. Burah
{"title":"Production and optimization of single cell protein from orange peels by Saccharomyces cerevisiae","authors":"H. Laminu, M. Yakubu, H. Bashir, M. A. Milala, B. Burah","doi":"10.31248/JBBD2018.081","DOIUrl":"https://doi.org/10.31248/JBBD2018.081","url":null,"abstract":"Deficiency in protein is increasing worldwide especially in developing countries. Protein from microbial biomass termed as Single Cell Protein (SCP) can be considered as an alternative to conventional source of food. This research work was carried out to produce single cell protein from orange peels by Saccharomyces cerevisiae isolated from spoilt tomato fruit and to optimize cultural condition in order to obtain a maximum amount of biomass concentration thus, the protein quantity. The proximate content of the peel was analysed using standard method. Carbohydrates was 59.96%, fibre (14.03%), moisture (12.07%), fat (9.11%), ash (5.21%) and protein (1.02%). Results of this study revealed that the optimum pH in which a maximum growth (1790 ppm) of biomass was obtained was at pH 7.0 after 120 hours of incubation. The effect of Incubation temperature showed that maximum biomass growth (1145 ppm) was obtained at 37°C after 96 hours of incubation. Effect of substrate concentration revealed that biomass concentration increased with increase in substrate concentration. Maximum protein with minimal nucleic acid content was obtained at incubation temperature of 37°C. In conclusion, it may not be favourable to incubate the fermentation medium at temperature above 55°C and pH below 4.0 for production of SCP using Saccharomyces cerevisiae.","PeriodicalId":15121,"journal":{"name":"Journal of Bioscience and Biotechnology Discovery","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83269615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of fermented cassava (Manihot esculenta) peel meal on the growth of Clarias gariepinus","authors":"A. A. Adewumi","doi":"10.31248/JBBD2018.070","DOIUrl":"https://doi.org/10.31248/JBBD2018.070","url":null,"abstract":"An experiment was conducted to evaluate the impact of cassava (Manihot esculenta, (NR 8082) peel, fermented with wastewater from the fermented cassava pulp, as a substitute for maize in the diet of C. gariepinus juveniles on the growth performance of C. gariepinus. Five iso-caloric and iso-nitrogenous diets were formulated containing 25%, 50%, 75% and 100% maize replacement with cassava peel meal (tagged diets B25, C50, D75, E100). The control was tagged diet A0. Two sets of experiment were designed using the formulated diets. One set, which lasted for 10 days, was designed to monitor the apparent protein digestibility of the diet and the second one to evaluate the growth, nutrient utilization (MWG, FCR, SGR) and the biochemical composition of the fillets of the fish, fed for a period of 8 weeks, in the laboratory. Fermentation significantly reduced (p 0.05) higher than those of the fish fed diets B25 and C50, but were significantly (P 0.05) from one another. The results revealed that 50% replacement of maize with cassava peel meal (diet C50) can be tolerated by C. gariepinus, without compromising growth, nutrient utilization and digestibility. The percentage survival of the fish fed the control, test diets B25, C50 and D75 were not significantly different (p>0.05) but were significantly different from those fish fed diet E100. It can be concluded that replacing maize with up to 50% replacement level of cassava peels fermented with wastewater from the fermented cassava pulp is not deleterious to growth and nutrient utilization of C. gariepinus. Fermentation might have thus reduced the toxicity and increased the nutrient value of the cassava peels, otherwise regarded as agricultural wastes, and placed it among basal feedstuffs, suitable for replacing maize in C. gariepinus diet.","PeriodicalId":15121,"journal":{"name":"Journal of Bioscience and Biotechnology Discovery","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85021310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Abdullahi, S. Eke, I. Omalu, I. Olayemi, E. Egwim, C. A. Otuu, S. Hassan, A. Boyi
{"title":"Malaria Parasitaemia among patients attending General Hospital Minna, North Central Nigeria","authors":"M. Abdullahi, S. Eke, I. Omalu, I. Olayemi, E. Egwim, C. A. Otuu, S. Hassan, A. Boyi","doi":"10.31248/JBBD2018.069","DOIUrl":"https://doi.org/10.31248/JBBD2018.069","url":null,"abstract":"Malaria is one of the most widespread parasitic infections globally and is a major cause of mortality, particularly in regions of high malaria endemicity. This study was carried out to investigate the prevalence of malaria infection and parasitaemia among patients attending General Hospital, Minna, North Central Nigeria. A total of one thousand and seventy-one (1,071) individuals within the study area were enrolled for the study from the month of June, 2016 to May, 2017. Malaria parasitaemia was carried out using thick and thin blood films. Parasites counts were reported per 800 white blood cells. The ages of the individuals recruited for the study ranged from 0 to 51years of age. Out of the 1,071 (100%) blood samples collected, 741 (69.19%) were positive for malaria parasitaemia of which 74 (9.99%), 134 (18.08%), 176 (23.75%) and 357 (48.18%) individuals scored malaria parasite densities of +++, ++, + and scanty had parasites in their blood stream respectively. Statistically, there was a significant difference (P 0.05) between the degree of malaria parasitaemia in relation to gender (sex). The high prevalence of malaria parasitaemia among patients attending General Hospital, Minna, North Central Nigeria highlights the need for more efforts to be targeted at controlling malaria among individuals as this will ultimately lead to a reduction in the incidence of malaria in Minna and Nigeria at large.","PeriodicalId":15121,"journal":{"name":"Journal of Bioscience and Biotechnology Discovery","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89555355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Okolie, M. O. Oguntokun, R. O. Ogboru, O. D. Idugboe, K. Nwaokobia
{"title":"Evaluation of the chemical composition of Persea americana (Mill) pulp and seed","authors":"P. Okolie, M. O. Oguntokun, R. O. Ogboru, O. D. Idugboe, K. Nwaokobia","doi":"10.31248/JBBD2018.071","DOIUrl":"https://doi.org/10.31248/JBBD2018.071","url":null,"abstract":"The chemical composition of Persea americana pulp and seed was investigated. Edible and non-edible parts of the fruits (pulp and seeds) were compared considering their possible role in improving the sustainability of the food and pharmaceutical industries. The results obtained showed that the investigated samples contain minerals such as carbon (17.29±0.03), hydrogen (0.65±0.07), nitrogen (4.13±0.01), oxygen (76.88±0.09), sodium (51.47±0.02), potassium (28.02±0.01), calcium (27.13±0.02), magnesium (40.77±0.01), Iron (12.01±0.01), zinc (7.23±0.01), phosphorus (31.63±0.04) and sulphur (0.04±0.01) was found in the pulp while silicon, aluminum and chlorine was not detected. Proximate composition revealed that the seed of P. americana contains more of volatile matter and fixed carbon of 27.55±0.01 and 58.35±0.04 respectively; while the pulp has more moisture content of 81.69±0.01 and ash of 1.02±0.01. Ultimate analysis showed that the seed has more carbon and oxygen while the pulp has more nitrogen and hydrogen. Mineral contents of the investigated samples higher in seed than in pulp in terms of concentration. These various chemical compositions of the evaluated samples may be behind their importance in the therapeutical, cosmetic and pharmaceutical industry.","PeriodicalId":15121,"journal":{"name":"Journal of Bioscience and Biotechnology Discovery","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78609236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. C. Otuu, M. Lazarus, S. Hassan, O. Michael, S. Eke, J. Luka, E. Peters
{"title":"Assessment of indoor residual spraying and entomological collections of mosquitoes at the student village of Nasarawa State University Keffi, Nasarawa State, Nigeria","authors":"A. C. Otuu, M. Lazarus, S. Hassan, O. Michael, S. Eke, J. Luka, E. Peters","doi":"10.31248/JBBD2018.061","DOIUrl":"https://doi.org/10.31248/JBBD2018.061","url":null,"abstract":"Mosquitoes are found around our surroundings as they cause public disturbances whether harmful or beneficial. Mosquitoes are insect vectors which transmit many dreadful diseases causing serious health problems to human being. In this study, two sampling sites were selected, mud and concrete houses. Indoor mosquitoes were collected with the use of knock-down pyrethrum insecticide and morphological identification was done in the Insectary laboratory with the aid of a dissecting microscope, using standard procedures. A total of 748 adult mosquitoes were caught, out of which a higher ravid with 33(9.48%) eache Abstract aboven be deduced from teh ely and carry our life acnumber of 400 (53.48%) mosquitoes were caught in mud houses while 348 (46.52%) were caught in concrete houses. Higher numbers of Culex sp were recorded in both mud houses and concrete houses. 345(51.96%) of the 400 mosquitoes from the mud houses were Culex sp while 55(65.48%) were Anopheles sp. Out of the 348 mosquitoes caught in the concrete houses, 319(48.04%) were Culex sp while 29(34.52%) were Anopheles. A highest number, 278(37.17%), of mosquitoes were caught in the month of July while the least number, 234(31.28%), was recorded in the month of June. Statistically, there was a significant difference between the relative abundance and distribution of mosquitoes based on months (P<0.05). The result of this study indicates the level of pollution in the area since Cullicines breed significantly in dirty stagnant water and due to the lack of proper drainage and sewage system in the study area. This can pose a serious health threat to the inhabitant. Health education will go a long way in sensitizing the inhabitants of the study area to avoid dumping of refuse indiscriminately.","PeriodicalId":15121,"journal":{"name":"Journal of Bioscience and Biotechnology Discovery","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82495334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Toxicity and oviposition inhibitory effect of extract and powder of Momordica charantia leaf against Callosobruchus maculatus Fab. (Coleoptera: Chrysomelidae) on stored cowpea seed","authors":"O. M. Obembe, D. O. Ojo","doi":"10.31248/JBBD2018.067","DOIUrl":"https://doi.org/10.31248/JBBD2018.067","url":null,"abstract":"The pulse beetles, Callosobruchus maculatus is a principal field-to store pest of cowpea in the tropics and at present synthetic insecticides constitute a major means of control. The insecticidal effect of ethanolic extracts and powders of Momordica charantia leaf against the cowpea beetle, Callosobruchus maculatus Fab. were carried out in the laboratory. The efficacy of powder and ethanolic extract of M. charantia leaf as toxicants against C. maculatus were investigated using contact toxicity and oviposition deterrence. The powders and extracts were applied at various dosages of 0.2, 0.4, 0.6, and 0.8 g and 0.2, 0.4, 0.6 and 0.8 ml per 20 g of cowpea seeds. Beetle mortality was monitored for 96 h. The mortality of the insect increased with increase in dosage and period of exposure. Within 24 h post treatment, 0.8 g dosage of the plant powder recorded the highest beetle mortality of 35% but its effect was not significantly (p>0.05) different from that of 0.4 and 0.6 ml. At 96 h of post-treatment, all the dosages of the plant powders recorded above 75% insect mortality with 0.8 g of the powder achieving the highest insect mortality of 85% and its effect was not significantly (p<0.05) different from 0.2, 0.4 and 0.6 g. At 96 h post treatment, 0.8 ml of the extract achieved the highest mortality of 95% which was not significantly (p<0.05) different from that of 0.6 ml. All dosages of the extract recorded up to 80% bruchid mortality within 96 h post treatment. At all treatment levels, insect mortality was significantly (p<0.05) different from the control. It was observed that oviposition was reduced in both powder and extract treatments when compared to the control. The results show that both powder and extract of M. charantia were effective in controlling C. maculatus and could serve as alternative to over-dependence on synthetic insecticide for preservation of stored cowpea seeds against C. maculatus","PeriodicalId":15121,"journal":{"name":"Journal of Bioscience and Biotechnology Discovery","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81055308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}