Journal of Arthropod-Borne Diseases最新文献

{"title":"Anti-Malarial Activity of Nano Tannic Acid MgO Extract Alone and Combined with Chloroquine against <i>Plasmodium berghei</i>.","authors":"Alireza Naziri, Afsaneh Motevali-Haghi, Mehdi Nateghpour, Mohammad Shabani, S Ahmad Dehdast, Abbas Rahimi-Foroushani, Aram Khezri, Zahra Farzaneh","doi":"10.18502/jad.v18i3.18577","DOIUrl":"10.18502/jad.v18i3.18577","url":null,"abstract":"<p><strong>Background: </strong>The global rise in malaria parasite resistance to antimalarial drugs necessitates new therapeutic strategies. Medicinal plant extracts, long used in traditional malaria treatment, have shown antiplasmodial potential in recent studies. This study investigated the effects of nano tannic acid MgO (NTA MgO) and chloroquine (CQ), both individually and in combination, on a chloroquine-sensitive <i>Plasmodium berghei</i> strain.</p><p><strong>Methods: </strong>BALB/c mice infected with <i>P. berghei</i> were divided into 11 groups. Groups were treated with NTA MgO (12.5, 25, 50, 100 mg/kg), CQ (1, 3, 10, 20 mg/kg), pure tannic acid (100 mg/kg), or assigned as controls. Peter's method determined the fifty percent effective dose (ED50) for NTA MgO and CQ. Drug interactions were assessed using the fixed-ratio method (ratios: 100/0, 90/10, 70/30, 50/50, 30/70, 10/90, 0/100). Parasitemia and inhibition percentages were calculated and analysed using SPSS software.</p><p><strong>Results: </strong>The ED₅₀ values for CQ and NTA MgO were found to be 1.1 mg/kg and 25 mg/kg, respectively. A synergistic effect was observed when a combination of 30% CQ and 70% NTA MgO was used, which significantly reduced parasitemia compared to the control group (P< 0.05, Kruskal-Wallis test). Additionally, NTA MgO administered alone at a dosage of 25 mg/kg effectively reduced the parasite load.</p><p><strong>Conclusion: </strong>NTA MgO showed strong antiplasmodial activity both alone and with chloroquine (CQ). The 30% CQ and 70% NTA MgO combination exhibited a significant synergistic effect, highlighting its potential as a new treatment for chloroquine-sensitive malaria and the promise of plant-based nanoparticles against drug-resistant malaria.</p>","PeriodicalId":15095,"journal":{"name":"Journal of Arthropod-Borne Diseases","volume":"18 3","pages":"264-275"},"PeriodicalIF":0.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification and Molecular Characterization of a Mammalian Neurotoxin as a Pharmaceutical Tool from the Venom of Iranian Scorpion <i>Androctonus crassicauda</i>.","authors":"Hadi Rabiei, Abbas Zare-Mirakabadi, Bita Mohtat, Behrooz Mirza","doi":"10.18502/jad.v18i3.18575","DOIUrl":"10.18502/jad.v18i3.18575","url":null,"abstract":"<p><strong>Background: </strong>Venom of scorpions are complex bioactive polypeptides. To gain greater insights into the structural and functional impacts of toxins from <i>Androctonus crassicauda</i> (Buthidae)<i>,</i> a dangerously venomous scorpion species, its venom was isolated, purified, and characterized.</p><p><strong>Methods: </strong>Long chain toxin with four disulfide bonds purified by size exclusion chromatography and reversed-phase HPLC and characterized by amino acid sequencing and molecular weight determination.</p><p><strong>Results: </strong>The primary structure analysis exhibits a neurotoxin named AnCra2 with 7302.24 Da molecular weight and 64 amino acid residues that cause paralysis and lead to death in NIH mice. The LD₅₀ of AnCra2 was determined to be 0.61±0.04 μg/mice. Phylogenetic analysis displays the toxin has 97% sequence similarity with alpha toxins reported from north African scorpions that affect voltage-gated sodium channels (VGSC), also proposed that differentiation among the scorpions of family Buthidae is affected by the geographical conditions and efficiency in evolutionary variations. AnCra2 exposed binding residues have a high affinity for receptor residues in site-3 (segment-3) of VGSC that are approved by three-dimensional structure and homology modeling.</p><p><strong>Conclusion: </strong>Purified AnCra2 seems to be a new putative Alpha neurotoxin in homology with the structure of neurotoxins that act on VGSC as a pharmaceutical tool.</p>","PeriodicalId":15095,"journal":{"name":"Journal of Arthropod-Borne Diseases","volume":"18 3","pages":"238-252"},"PeriodicalIF":0.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dengue in Bangladesh: A Gendered Perspective on Infection and Fatality Rates amidst Global Epidemiological Trends.","authors":"Marjana Akter, Yusha Araf, Sharmin Akter, Md Golzar Hossain","doi":"10.18502/jad.v18i3.18579","DOIUrl":"10.18502/jad.v18i3.18579","url":null,"abstract":"","PeriodicalId":15095,"journal":{"name":"Journal of Arthropod-Borne Diseases","volume":"18 3","pages":"281-284"},"PeriodicalIF":0.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Characterization and Phylogenetic Analysis of Flea Species in Human and Livestock Residence by Targeting ITS2 Region in East Azerbaijan Province, Iran.","authors":"Mostafa Farmani, Adel Spotin, Afsaneh Dolatkhah, Saber Gholizadeh, Teimour Hazratian","doi":"10.18502/jad.v18i3.18571","DOIUrl":"10.18502/jad.v18i3.18571","url":null,"abstract":"<p><strong>Background: </strong>Fleas are blood-sucking ectoparasites with complete metamorphosis. They belong to the order Siphonaptera and can infest both humans and animals, causing dermatitis and transmitting vector-borne diseases. Despite extensive study of their classification and biology, the phylogenetic relationship between fleas in Iran is not fully understood. This research aimed to identify the flea species collected from different parts of East Azerbaijan Province in northwest Iran, using morphological, molecular, and phylogenetic analysis.</p><p><strong>Methods: </strong>From October 2019 to October 2020, we collected fleas using various methods such as hand catching for humans, brushing for dogs and cats, sticky traps for rodent burrows, light traps, and dishes with water for sheep and goats. After identifying the flea species using morphological identification keys, we extracted total genomic DNA and amplified it by targeting the ITS2 region. The PCR products were then directly sequenced to investigate the flea species.</p><p><strong>Results: </strong>In total, 1929 flea specimens were collected, revealing three genera and four species. The breakdown of the specimens is as follows: <i>Pulex irritans</i> (n=1206; 62.5%), <i>Ctenocephalides canis</i> (n=345; 18%), <i>Ctenocephalides felis felis</i> (n=203; 10.5%), <i>Ctenocephalides felis orientis</i> (n=160; 8%), and <i>Xenopsylla nuttalli</i> (n=15; 1%). Phylogenetic analysis indicated low to moderate haplotype diversity (Hd: 0-0.524) across five distinct clades: <i>P. irritans</i>, <i>C. canis</i>, <i>C. felis felis</i>, <i>C. felis orientis</i>, and <i>X. nuttalli</i>.</p><p><strong>Conclusion: </strong>This study represents the first in-depth analysis in East Azerbaijan Province, highlighting the significance of considering <i>P. irritans</i> as a major vector when assessing the risk of local disease transmission.</p>","PeriodicalId":15095,"journal":{"name":"Journal of Arthropod-Borne Diseases","volume":"18 3","pages":"180-188"},"PeriodicalIF":0.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discrimination of <i>Phlebotomus perfiliewi transcaucasicus</i>, <i>Ph. major</i> sensu lato and <i>Ph. tobbi</i> (Diptera: Psychodidae) Using Morphometric and DNA Barcoding Methods in the Endemic Foci of Visceral Leishmaniasis in Ardabil Province, North West of Iran.","authors":"Alireza Barmaki, Yavar Rassi, Azad Absavaran, Amir Ahmad Akhavan, Eslam Moradi-Asl, Alireza Zahraei-Ramazani, Sayana Rafizadeh","doi":"10.18502/jad.v18i3.18573","DOIUrl":"10.18502/jad.v18i3.18573","url":null,"abstract":"<p><strong>Background: </strong>Visceral leishmaniasis, commonly known as kala-azar, and prevalent in more than 70 countries and several regions of Iran. It is one of the main diseases transmitted by sand flies. In this work, geometric morphometrics and DNA barcoding were employed as novel techniques to enhance the diagnostic tools used in this study.</p><p><strong>Methods: </strong><i>Phlebotomus perfiliewi transcaucasicus</i>, <i>Phlebotomus major</i> s.l., and <i>Phlebotomus tobbi</i> caught from three districts in the Ardabil Province, northwest of Iran. The right wings of 286 female sand flies were analyzed using geometric morphometric (GM) tools. Additionally, the COI gene was isolated from each of the three species, amplified using universal primers, and sequenced through the DNA barcoding method for classification. This sequencing data was then formatted to generate morphometric analyses.</p><p><strong>Results: </strong>The landmarks with the most variations were found in sets 10, 12, 13, and 14, whereas the first set's landmarks at 1 and 11, along with those from the second set at positions 2, 3, and 5 exhibited the greatest variations. Analysis of the size and shape variations in the wings indicates the presence of distinct populations (P< 0.05). Furthermore, the DNA barcoding results not only confirmed the findings from the geometric morphometric analysis but also revealed both interspecific and intraspecific distances.</p><p><strong>Conclusion: </strong>This study was the first attempt to assess whether wing geometry morphometrics, combined with DNA barcode techniques, can effectively distinguish the three mentioned species in the studied areas. Furthermore, the identification of <i>Phlebotomus neglectus</i> in this area prompted recommendations for additional research.</p>","PeriodicalId":15095,"journal":{"name":"Journal of Arthropod-Borne Diseases","volume":"18 3","pages":"197-217"},"PeriodicalIF":0.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Five-Year Trend of Malaria Parasitemia with Coverage and Use of the Major Control Interventions in Itang District, Gambella Region, Western Ethiopia (2017-2021).","authors":"Bolgony Bonker, Oljira Kenea, Damtew Bekele","doi":"10.18502/jad.v18i3.18574","DOIUrl":"10.18502/jad.v18i3.18574","url":null,"abstract":"<p><strong>Background: </strong>One of the main global public health issues that affect several facets of the population is malaria. Ethiopia primarily uses Indoor Residual Spraying (IRS) and Long-lasting Insecticidal Nets (LLINs) as lifesaving frontline malaria vector prevention and control interventions. This study intends to assess the trend of malaria prevalence and coverage and utilization of IRS and LLINs in Itang district, western Ethiopia.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on malaria cases reported by Health Centers from 2017 to 2021. Data were gathered between July 02, 2021, and October 30, 2021, using a community-based cross-sectional household survey to evaluate the coverage and utilization of IRS and LLINs. The chi-square test was used to compare the trend of malaria prevalence among sex and age groups. Statistical significance was considered at P< 0.05.</p><p><strong>Results: </strong>Among 4,500 patients with suspected cases of malaria, 40% of the cases had microscopically confirmed malaria. <i>Plasmodium falciparum</i> was more prevalent than <i>P. vivax</i> in the district. Only 368 (38.25%) people slept under the net yesterday night out of the 962 people who utilized it overall during the study period. 127 (63.5%) households reported no insecticide spraying in the past 12 months, while 73 (36.5%) reported their houses were sprayed.</p><p><strong>Conclusion: </strong>The number of malaria cases reached its peak in 2019 and hit its lowest point in 2021. Results show that IRS and LLINs coverage and utilization were poor and warrant community awareness and support by all concerned bodies.</p>","PeriodicalId":15095,"journal":{"name":"Journal of Arthropod-Borne Diseases","volume":"18 3","pages":"218-237"},"PeriodicalIF":0.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fatal Crimean-Congo Hemorrhagic Fever with an Atypical Clinical Course during the COVID-19 Pandemic.","authors":"Mustafa Arslan","doi":"10.18502/jad.v18i3.18578","DOIUrl":"10.18502/jad.v18i3.18578","url":null,"abstract":"<p><strong>Background: </strong>Crimean-Congo hemorrhagic fever (CCHF) is a viral zoonotic disease characterized by high fever, bleeding manifestations, and a high mortality rate. Most patients begin to experience bleeding 5 to 7 days after the onset of the illness, usually while hospitalized. This report highlights that even though patients may develop shock and multi-organ failure, there might be no visible signs of bleeding until shortly before death.</p><p><strong>Methods: </strong>This case report describes a 42-year-old male patient with a confirmed diagnosis of CCHF via RT-PCR, who died despite intensive care treatment. Despite receiving intensive care treatment, he did not respond to the septic shock therapy and unfortunately passed away within a few hours. The diagnosis of the disease was made by the reference laboratory with a positive reverse transcriptase-polymerase chain reaction (RT-PCR) test for CCHF.</p><p><strong>Results: </strong>In this case, the patient exhibited no visible signs of bleeding, such as hematemesis, melena, or gross hematuria, despite being in shock. While CCHF mortality (5-30%) typically occurs during the 3-5-day hemorrhagic phase, this patient died suddenly due to massive intra-abdominal bleeding, skipping the typical bleeding phase.</p><p><strong>Conclusion: </strong>Clinicians should consider that individual differences may be seen in the clinical course of CCHF disease. In addition, the Coronavirus Disease-2019 (COVID-19) pandemic was experienced worldwide when the patient applied. Given the overlapping initial symptoms of COVID-19 and CCHF, clinicians should prioritize differential diagnosis, especially during the COVID-19 pandemic. During the COVID-19 pandemic, diagnostic delays may occur in the management of CCHF, particularly due to resource allocation.</p>","PeriodicalId":15095,"journal":{"name":"Journal of Arthropod-Borne Diseases","volume":"18 3","pages":"276-280"},"PeriodicalIF":0.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Optimization of a Nanoparticle-Based Imidacloprid Insecticide for Effective Control of <i>Blattella germanica</i>.","authors":"Ismail Saba, Amir Amani, Mojgan Baniardalani, Hamid Reza Basseri","doi":"10.18502/jad.v18i2.17535","DOIUrl":"10.18502/jad.v18i2.17535","url":null,"abstract":"<p><strong>Background: </strong>The German cockroach (<i>Blattella germanica</i>) is a pest with a global distribution that has adapted to live in human environments. <i>Blattella germanica</i> threatens human health by producing asthma-inducing allergens, carrying pathogenic/antibiotic-resistant microbes, and contributing to unhealthy indoor environments. Effective application of insecticides can play an important role in cockroach control programs. The purpose of this research was to develop and optimize a nanoparticle-based imidacloprid insecticide and evaluate its effectiveness against the German cockroach.</p><p><strong>Methods: </strong>A bioassay was conducted to determine the LC₅₀ and LC₉₀ of imidacloprid technical against adult German cockroaches. The appropriate initial concentration of 3mg/m² was then utilized in the synthesis of nanoencapsulated imidacloprid via the ionic gelation method. The average particle size was determined using Dynamic Light Scattering (DLS) and the dried nanoparticles were analyzed using a Scanning Electron Microscope (SEM). The LC₅₀ and LC₉₀ values of Nano-imidacloprid were then compared with the technical grade of the insecticide.</p><p><strong>Results: </strong>A comparison of the bioassay results for nanoencapsulated and imidacloprid technical revealed a superior insecticidal effect of the nanoencapsulated imidacloprid against the German cockroach. The LC₅₀ value for the nanoencapsulated imidacloprid decreased from 4.656 to 3.081 mg/m² and the LC₉₀ value decreased from 8.381 to 4.486 mg/m² when compared to imidacloprid technical.</p><p><strong>Conclusion: </strong>The use of nanotechnology in insecticides can lead to increased efficacy and reduced consumption. This is because the smaller particle size of nanomaterials allows for better penetration and targeted delivery to pest organisms, reducing the overall amount needed for control.</p>","PeriodicalId":15095,"journal":{"name":"Journal of Arthropod-Borne Diseases","volume":"18 2","pages":"137-148"},"PeriodicalIF":0.6,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Severity of <i>Demodex folliculorum</i> and <i>Demodex brevis</i> Infestation and Their Association with Common Skin Diseases in Northwest Iran.","authors":"Milad Afşar, Khosrow Hazrati-Tappeh, Yunus Emre-Beyhan","doi":"10.18502/jad.v18i2.17533","DOIUrl":"10.18502/jad.v18i2.17533","url":null,"abstract":"<p><strong>Background: </strong><i>Demodex</i> spp., a cosmopolitan mite, can exist as a commensal or parasitic organism. This study aimed to investigate the prevalence of <i>Demodex</i> spp. infestation in patients with common skin diseases and explore the potential association between demodicosis and these conditions in Urmia, northwest Iran.</p><p><strong>Methods: </strong>A total of 246 patients attending the Skin Polyclinic of Iranian Urmia Taleghani Hospital were enrolled in the study. Samples were taken from the nose wings, cheeks, and forehead regions of the face area of the persons using the standard superficial skin biopsy method.</p><p><strong>Results: </strong>Demodicosis was detected in 43 (16.3%) patients, with 42 cases attributed to <i>D. folliculorum</i> and 1 case to <i>D. brevis</i> infestation. The highest positivity was found in the 31-50 (21.4%) and 51-72 (22.2%) age groups. In the relationship between demodicosis and skin diseases, <i>Demodex</i> spp. was found in 13 (32.5%) of 40 patients who had skin disease; 6 of these patients with rosacea (42.9%) and 7 with eczema (29.2%). Mite infestation was found in 6 (37.5%) of 16 patients who had hemodialysis due to renal insufficiency. Furthermore, 46.7% of positive patients reported using common goods. The prevalence of demodicosis was similar among patients residing in villages (17.2%) and cities (17.6%).</p><p><strong>Conclusion: </strong>This study sheds light on the potential association between <i>Demodex</i> infestation and common skin diseases such as rosacea and eczema in Urmia, Iran. Therefore, there is a pressing need to augment research endeavors on demodicosis.</p>","PeriodicalId":15095,"journal":{"name":"Journal of Arthropod-Borne Diseases","volume":"18 2","pages":"113-121"},"PeriodicalIF":0.6,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Comprehensive List of Phlebotomine Sand Fly Species (Diptera: Psychodidae) in a Leishmaniasis Focus (Djelfa, Algeria), Including the First Record of <i>Phlebotomus langeroni</i> Females in Algeria.","authors":"Fatma Messaoudene, Slimane Boukraa, Said Chaouki-Boubidi, Ahlem Guerzou","doi":"10.18502/jad.v18i2.17532","DOIUrl":"10.18502/jad.v18i2.17532","url":null,"abstract":"<p><strong>Background: </strong>The wide distribution of phlebotomine vectors complicates the leishmaniasis situation in the world, with the risk of spreading from rural to urban areas. Our study investigates for the first time the ecology and distribution of sand fly populations in leishmaniasis focus (Djelfa, Algeria).</p><p><strong>Methods: </strong>Sampling is performed using light traps from August 2021 to July 2022 at ten sites with different biotopes: two peri-urban stations (Ain Oussera and Hassi Bahbah), one urban station (Djelfa), and three rural stations (Ain El-Bel, Haniet Ouled Salem and Mlaga).</p><p><strong>Results: </strong>A total of 2,866 specimens with 12 species belonging to <i>Phlebotomus</i> and <i>Sergentomyia</i> were identified with the first record of <i>Phlebotomus langeroni</i> female nationally, revealing a good diversity of up to 1.812 bits. The greatest richness of species was found in rural sites at Ain El Bel station, while the lowest richness was observed in the first peri-urban site of Ain Oussera station. Interestingly, the surveyed species were evenly distributed across all the study sites, in particular <i>Phlebotomus papatasi</i> with 57.29%. Statistical treatment revealed a strong positive relationship between temperature and species (p< 0.001, r= 0.91). Considering the other factors, there was a moderate link between species richness and altitude (p= 0.01 R²= 0.519). However, neither rainfall nor wind speed (p> 0.05) were correlated with sand fly fauna. Moreover, humidity showed a negative correlation (p< 0.001, r= -0.96).</p><p><strong>Conclusion: </strong>The findings of this study on phlebotomine fauna in this endemic region for leishmaniasis provide valuable data to support the success of a vector control program.</p>","PeriodicalId":15095,"journal":{"name":"Journal of Arthropod-Borne Diseases","volume":"18 2","pages":"94-112"},"PeriodicalIF":0.6,"publicationDate":"2024-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}