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Journal of Biomolecular Screening最新文献

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Long Noncoding RNAs CAT2064 and CAT2042 may Function as Diagnostic Biomarkers for Prostate Cancer by Affecting Target MicrorRNAs. 长非编码 RNA CAT2064 和 CAT2042 可通过影响目标微小 RNA 发挥前列腺癌诊断生物标志物的作用。
Journal of Biomolecular Screening Pub Date : 2024-07-01 Epub Date: 2021-08-24 DOI: 10.1007/s12291-021-00999-6
Farzane Amirmahani, Nasim Ebrahimi, Rafee Habib Askandar, Marzieh Rasouli Eshkaftaki, Katayoun Fazeli, Michael R Hamblin
{"title":"Long Noncoding RNAs CAT2064 and CAT2042 may Function as Diagnostic Biomarkers for Prostate Cancer by Affecting Target MicrorRNAs.","authors":"Farzane Amirmahani, Nasim Ebrahimi, Rafee Habib Askandar, Marzieh Rasouli Eshkaftaki, Katayoun Fazeli, Michael R Hamblin","doi":"10.1007/s12291-021-00999-6","DOIUrl":"10.1007/s12291-021-00999-6","url":null,"abstract":"<p><p>Prostate cancer (PCa) is the second most common cancer in men throughout the world, and the main cause of cancer death. Long noncoding RNAs (lncRNAs) act as crucial regulators in many human cancers. In this research, we measured the expression level of novel lncRNAs and their associated micro-RNAs (miRNAs) in PCa. In the present research, three lncRNAs were selected using the Mitranscriptome projec (CAT2064, CAT2042, and CAT2164.2). Samples of prostate tissue (20 PCa, and 20 BPH) and blood (14 PCa, and 14 BPH) were collected and the Real-time Quantitative Polymerase Chain Reaction (RT-qPCR) was used to measure the expression levels of the lncRNAs and their associated miRNAs. Based on our results, CAT2064 was significantly increased and CAT2042 was significantly decreased in human PCa tissue in comparison with BPH tissue. To discriminate PCa from BPH, CAT2064 (<i>P</i> < 0.05; 0.8750 AUC-ROC) showed a better potential as a diagnostic molecular biomarker compared to CAT2042 (<i>P</i> < 0.05; 0.8454 AUC-ROC). Furthermore, RT-qPCR results measured in blood samples from PCa patients showed a higher expression level of CAT2064 (<i>P</i> < 0.0001; AUC-ROC value of 0.8914) in comparison to CAT2042. CAT2064 and CAT2042 showed a positive correlation with the expression of miR-5095 and miR-1273a (r = 0.02885, 0.3202; <i>P</i> = 0.9413, 0.2266, respectively). CAT2064 and CAT2042 also had a negative correlation with miR-1304-3p and miR-1285-5p (r = - 0.3877, - 0.09330; <i>P</i> = 0.15, 0.7311, respectively). Collectively, CAT2064 and CAT2042 and their miRNA targets may constitute a regulatory network in PCa, and could serve as novel biomarkers.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12291-021-00999-6.</p>","PeriodicalId":15087,"journal":{"name":"Journal of Biomolecular Screening","volume":"6 1","pages":"322-330"},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11239640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87379283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Novel Chemical Scaffolds for Inhibition of Rifamycin-Resistant RNA Polymerase Discovered from High-Throughput Screening. 高通量筛选发现抑制利福霉素耐药RNA聚合酶的新型化学支架。
Journal of Biomolecular Screening Pub Date : 2016-12-01 DOI: 10.1177/1087057116679994
N. Scharf, V. Molodtsov, A. Kontos, K. Murakami, G. Garcia
{"title":"Novel Chemical Scaffolds for Inhibition of Rifamycin-Resistant RNA Polymerase Discovered from High-Throughput Screening.","authors":"N. Scharf, V. Molodtsov, A. Kontos, K. Murakami, G. Garcia","doi":"10.1177/1087057116679994","DOIUrl":"https://doi.org/10.1177/1087057116679994","url":null,"abstract":"Rifampin has been a cornerstone of tuberculosis (TB) treatment since its introduction. The rise of multidrug-resistant and extensively drug-resistant TB makes the development of novel therapeutics effective against these strains an urgent need. Site-specific mutations in the target enzyme of rifampin, RNA polymerase (RNAP) comprises the majority (~97%) of rifamycin-resistant (RifR) strains of Mycobacterium tuberculosis (MTB). To identify novel inhibitors of bacterial RNAP, an in vitro plasmid-based transcription assay that uses malachite green (MG) to detect transcribed RNA containing MG aptamers was developed. This assay was optimized in a 384-well plate format and used to screen 150,000 compounds against an Escherichia coli homolog of the most clinically relevant RifR RNAP (βS531L) containing a mutation (β'V408G) that compensates for the fitness defect of this RifR mutant. Following confirmation and concentration-response studies, 10 compounds were identified with similar in vitro inhibition values across a panel of wild-type and RifR E. coli and MTB RNAPs. Four compounds identified from the screen are active against MTB in culture at concentrations below 200 µM. Initial follow-up has resulted in the elimination of one scaffold due to potential pan-assay interference.","PeriodicalId":15087,"journal":{"name":"Journal of Biomolecular Screening","volume":"1 1","pages":"1087057116679994"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1087057116679994","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65313127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Product Focus: Screening Robotics and Automation 产品重点:筛选机器人及自动化
Journal of Biomolecular Screening Pub Date : 2010-07-01 DOI: 10.1177/1087057110373266
P. Taylor
{"title":"Product Focus: Screening Robotics and Automation","authors":"P. Taylor","doi":"10.1177/1087057110373266","DOIUrl":"https://doi.org/10.1177/1087057110373266","url":null,"abstract":"© 2010 Society for Biomolecular Sciences Hamilton Storage Technologies announces the addition of the SAMple Puncher, an integrated tube-processing module that operates in conjunction with the manufacturer’s –20 °C and –80 °C Sample Access Manager (SAM) systems. The system handles and stores multiple labware types, is compatible with biobanking applications, tracks sample usage, uses 1D and 2D barcode reading, and has liquid nitrogen as a backup option. The system software provides audit trails and tools for chain of custody and 21 CFR Part 11 compliance. For further information, visit www.hamilton-storage.com. Hamilton Robotics announces the NIMBUS iProbe, with up to 4 independent channels that can be configured with combinations of 1or 5-mL pipetting heads. The unit can carry out tube-to-tube, tube-to-plate, and plate-to-plate transfers via a graphic user interface; can fit in a","PeriodicalId":15087,"journal":{"name":"Journal of Biomolecular Screening","volume":"15 1","pages":"710 - 714"},"PeriodicalIF":0.0,"publicationDate":"2010-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1087057110373266","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65311522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Product Focus: High-Content Screening and Imaging 产品重点:高含量筛选和成像
Journal of Biomolecular Screening Pub Date : 2010-05-28 DOI: 10.1177/1087057110368517
C. Hart
{"title":"Product Focus: High-Content Screening and Imaging","authors":"C. Hart","doi":"10.1177/1087057110368517","DOIUrl":"https://doi.org/10.1177/1087057110368517","url":null,"abstract":"","PeriodicalId":15087,"journal":{"name":"Journal of Biomolecular Screening","volume":"15 1","pages":"584 - 589"},"PeriodicalIF":0.0,"publicationDate":"2010-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1087057110368517","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65311501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Product Focus 产品集中
Journal of Biomolecular Screening Pub Date : 2008-10-01 DOI: 10.1177/10870571080130091501
{"title":"Product Focus","authors":"","doi":"10.1177/10870571080130091501","DOIUrl":"https://doi.org/10.1177/10870571080130091501","url":null,"abstract":"Cellectricon has introduced the Cellaxess®HT System, an automated system for reagent-free transfection that enables reagent-free delivery of siRNA and cDNA to host cells at a throughput of 50,000 wells per day. The system uses capillary electrode arrays for reagent-free transfection of cells directly in 384-well plates. The plates are designed for high-content screening (HCS) readout and are compatible with","PeriodicalId":15087,"journal":{"name":"Journal of Biomolecular Screening","volume":"13 1","pages":"922 - 927"},"PeriodicalIF":0.0,"publicationDate":"2008-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/10870571080130091501","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65310843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Product Focus 产品集中
Journal of Biomolecular Screening Pub Date : 2008-09-01 DOI: 10.1177/10870571080130081201
{"title":"Product Focus","authors":"","doi":"10.1177/10870571080130081201","DOIUrl":"https://doi.org/10.1177/10870571080130081201","url":null,"abstract":"BioTek Instruments, Inc. announced a new software tool for clinical laboratories using its ELx800 and ELx808 microplate readers. The data collection and analysis software offer preprogrammed clinical diagnostic assays in a PC-based application. The PC-based interface provides flexibility in data analysis and output options and includes LIS system compatibility and data/protocol storage. In addition,","PeriodicalId":15087,"journal":{"name":"Journal of Biomolecular Screening","volume":"13 1","pages":"822 - 826"},"PeriodicalIF":0.0,"publicationDate":"2008-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/10870571080130081201","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65310782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Product Focus: Screening Robotics and Automation 产品重点:筛选机器人及自动化
Journal of Biomolecular Screening Pub Date : 2008-07-01 DOI: 10.1177/10870571080130061201
{"title":"Product Focus: Screening Robotics and Automation","authors":"","doi":"10.1177/10870571080130061201","DOIUrl":"https://doi.org/10.1177/10870571080130061201","url":null,"abstract":"BioTek Instruments has announced the Synergy 4 multidetection microplate reader, which combines a fluorescence filter-based detection system and a monochromator-based detection system in 1 unit. The filter-based system can be used when high sensitivity or fast read speeds are a requirement. The monochromator system is available when spectral scanning is needed. This modular reader can be ordered fully loaded with all measurement modes or customized to fit budgetary requirements with the option to add other detection technologies at a later date. Available options include filterbased and monochromator-based fluorescence intensity, time-resolved fluorescence, fluorescence polarization, UV-visible absorbance, flash and glow luminescence, and a dual-reagent","PeriodicalId":15087,"journal":{"name":"Journal of Biomolecular Screening","volume":"13 1","pages":"544 - 548"},"PeriodicalIF":0.0,"publicationDate":"2008-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/10870571080130061201","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65310718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Book Review: Immunoassay and Other Bioanalytical Techniques, edited by Jeanette M. Van Emon. Boca Raton, FL: CRC Press, Taylor & Francis Group; 2007. 512 pp. Hardcover. ISBN 10:0—8493—3942—1 书评:免疫测定和其他生物分析技术，由Jeanette M. Van Emon编辑。博卡拉顿，佛罗里达州:CRC出版社，泰勒和弗朗西斯集团;2007. 512页，精装。ISBN 10:0 - 8493 - 3942 - 1
Journal of Biomolecular Screening Pub Date : 2008-06-01 DOI: 10.1177/1087057108317441
T. Chung
{"title":"Book Review: Immunoassay and Other Bioanalytical Techniques, edited by Jeanette M. Van Emon. Boca Raton, FL: CRC Press, Taylor & Francis Group; 2007. 512 pp. Hardcover. ISBN 10:0—8493—3942—1","authors":"T. Chung","doi":"10.1177/1087057108317441","DOIUrl":"https://doi.org/10.1177/1087057108317441","url":null,"abstract":"","PeriodicalId":15087,"journal":{"name":"Journal of Biomolecular Screening","volume":"29 1","pages":"430 - 431"},"PeriodicalIF":0.0,"publicationDate":"2008-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1087057108317441","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65310693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Product Focus: Software, Databases, and Information Services 产品重点:软件、数据库和信息服务
Journal of Biomolecular Screening Pub Date : 2008-03-01 DOI: 10.1177/10870571080130030901
{"title":"Product Focus: Software, Databases, and Information Services","authors":"","doi":"10.1177/10870571080130030901","DOIUrl":"https://doi.org/10.1177/10870571080130030901","url":null,"abstract":"Guava Technologies Inc. has introduced a new generation of software for its Guava EasyCyteTM Plus microcapillary flow cytometry system. Designed for both data acquisition and analysis, the software interface allows you to visualize up to 8 plots simultaneously while still accessing operation or data analysis functions. With a right click of the mouse, features including the plot type, marker selection, statistics, gates, and other functions can be customized to your experimental design. Color-coded dots allow you to follow your gating strategies across multiple plots and","PeriodicalId":15087,"journal":{"name":"Journal of Biomolecular Screening","volume":"13 1","pages":"246 - 248"},"PeriodicalIF":0.0,"publicationDate":"2008-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/10870571080130030901","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65310627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Product Focus: Microplates, Assay Reagents, Screening Consumables, and Kits 产品重点:微孔板，化验试剂，筛选耗材和试剂盒
Journal of Biomolecular Screening Pub Date : 2008-02-01 DOI: 10.1177/10870571080130021101
{"title":"Product Focus: Microplates, Assay Reagents, Screening Consumables, and Kits","authors":"","doi":"10.1177/10870571080130021101","DOIUrl":"https://doi.org/10.1177/10870571080130021101","url":null,"abstract":"","PeriodicalId":15087,"journal":{"name":"Journal of Biomolecular Screening","volume":"13 1","pages":"170 - 173"},"PeriodicalIF":0.0,"publicationDate":"2008-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/10870571080130021101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65310615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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