Journal of bioluminescence and chemiluminescence最新文献_第3页

Bioluminescence and chemiluminescence literature. The 1995 literature. Part 2. 生物发光和化学发光文献。1995年的文献。第2部分。

Journal of bioluminescence and chemiluminescence Pub Date : 1996-01-01 DOI: 10.1002/(SICI)1099-1271(199601)11:1<39::AID-BIO401>3.0.CO;2-P

L J Kricka, P E Stanley

引用次数: 1

Weak chemiluminescence of bilirubin and its stimulation by aldehydes. 胆红素的弱化学发光及其醛类的刺激。

Journal of bioluminescence and chemiluminescence Pub Date : 1992-01-01 DOI: 10.1002/bio.1170070102

H Watanabe, M Usa, M Kobayashi, S Agatsuma, H Inaba

{"title":"Weak chemiluminescence of bilirubin and its stimulation by aldehydes.","authors":"H Watanabe, M Usa, M Kobayashi, S Agatsuma, H Inaba","doi":"10.1002/bio.1170070102","DOIUrl":"https://doi.org/10.1002/bio.1170070102","url":null,"abstract":"Bilirubin in an alkaline solution exhibits a weak chemiluminescence (CL) under aerobic conditions. This spontaneous CL was markedly enhanced by the addition of various aldehydes. The fluorescent emission spectrum of bilirubin, excited by weak intensity light at 350 nm, coincided with its CL emission spectrum (peak at 670 nm). CL emission from bilirubin was not quenched by active oxygen scavengers. This suggests that triplet oxygen reacts with bilirubin, and forms an oxygenated intermediate (hydroperoxide) as a primary emitter (oxidative scission of tetrapyrrole bonds in bilirubin is not involved in this CL). The Ehrlich reaction (test for monopyrroles) and hydrolsulphite reaction (test for dipyrroles) on the CL reaction mixture and unreacted bilirubin showed no differences. When the CL was initiated by singlet oxygen, rather than superoxide anion, monopyrrole, was detected in the reaction products by gel chromatography. The inhibitory effect of a scavenger of singlet oxygen on CL was eliminated in the presence of formaldehyde. Therefore, triplet carbonyl, formed by singlet oxygen through the dioxetane structure in bilirubin, is not an emitter. The reaction mechanism of bilirubin CL and the formation of a hydroperoxide intermediate is discussed in relation to the chemical structure of luciferin molecules from bioluminescent organisms.","PeriodicalId":15068,"journal":{"name":"Journal of bioluminescence and chemiluminescence","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/bio.1170070102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12496174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The effect of serine and thiol protease inhibitors on the chemiluminescence of human neutrophils in investigations in vitro. 丝氨酸和巯基蛋白酶抑制剂对人中性粒细胞体外化学发光的影响。

Journal of bioluminescence and chemiluminescence Pub Date : 1992-01-01 DOI: 10.1002/bio.1170070106

J Kantorski, H Tchórzewski

{"title":"The effect of serine and thiol protease inhibitors on the chemiluminescence of human neutrophils in investigations in vitro.","authors":"J Kantorski, H Tchórzewski","doi":"10.1002/bio.1170070106","DOIUrl":"https://doi.org/10.1002/bio.1170070106","url":null,"abstract":"We have studied an indirect role of serine and thiol proteases in the activation of human neutrophils in vitro. Stimulation was evaluated using a chemiluminescence (CL) generation system. Receptor-dependent and receptor-independent stimuli were studied, e.g. opsonized zymosan, formyl-methionyl-leucyl-phenylalanine, platelet activating factor, phorbol myristate acetate, and calcium ionophore A23187. The serine protease inhibitors TPCK and TLCK, and thiol protease inhibitor PHMB, diminished the CL with different potencies and in a dose-dependent manner after treatment of cells with the various stimuli. Non-specific serine protease inhibitor, PMSF, and trypsin substrate TAME, showed a low inhibitory potency with respect to CL generation. Synthetic substrates for chymotrypsin (BTEE, ATEE) significantly inhibited CL with the various stimuli used with some differences in susceptibility to their inhibition. Specific chymotrypsin inhibitors diminished both the resting and activator-induced CL. We suggest that cell-bound chymotrypsin-like protease(s) is involved in the activation of signal transduction in human neutrophils after both receptor-dependent and receptor-independent stimulation.","PeriodicalId":15068,"journal":{"name":"Journal of bioluminescence and chemiluminescence","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/bio.1170070106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12808437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Excited carbonyl formation in the combination and disproportionation of free radicals. 自由基结合和歧化过程中激发羰基的形成。

Journal of bioluminescence and chemiluminescence Pub Date : 1992-01-01 DOI: 10.1002/bio.1170070105

J R de la Fuente, E A Lissi

引用次数: 7

Enhanced chemiluminescence in the oxidation of luminol and an isoluminol cortisol conjugate by hydrogen peroxide in reversed micelles. 过氧化氢在反胶束中氧化鲁米诺和异米诺皮质醇偶联物的化学发光增强。

Journal of bioluminescence and chemiluminescence Pub Date : 1992-01-01 DOI: 10.1002/bio.1170070104

D I Metelitza, A N Eryomin, V A Shibaev

引用次数: 7

Bilirubin chemiluminescence induced by the attack of active oxygen species. 活性氧攻击诱导的胆红素化学发光。

Journal of bioluminescence and chemiluminescence Pub Date : 1992-01-01 DOI: 10.1002/bio.1170070103

H Watanabe, T Nagoshi, S Agatsuma, M Kobayashi, H Inaba

{"title":"Bilirubin chemiluminescence induced by the attack of active oxygen species.","authors":"H Watanabe, T Nagoshi, S Agatsuma, M Kobayashi, H Inaba","doi":"10.1002/bio.1170070103","DOIUrl":"https://doi.org/10.1002/bio.1170070103","url":null,"abstract":"Ultraweak chemiluminescence (CL) from bilirubin occurs in the presence of triplet oxygen and is stimulated by the addition of aldehydes. Active oxygen species also enhance bilirubin CL, in the absence of aldehydes. An inhibitory effect of active oxygen scavengers on the CL indicated that active oxygens generated from the decomposition of added hydrogen peroxide or from the xanthine-xanthine oxidase reaction contributed to the CL from bilirubin molecules. However, the contribution of singlet oxygen to the CL disappeared in the presence of formaldehyde. This suggested that the scission of tetrapyrrole bonds via a dioxetane intermediate or the production of triplet carbonyls from the oxidation of aldehydes by singlet oxygen was not involved in the CL, at least in the presence of formaldehyde. The spectrum of CL induced by the generation of active oxygen was the same as that from the aldehyde-enhanced CL reaction. We propose that the formation of a hydroperoxide (and/or hydroxide) bilirubin intermediate, but not a dioxetane, may be involved in the excitation of bilirubin molecules for CL.","PeriodicalId":15068,"journal":{"name":"Journal of bioluminescence and chemiluminescence","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/bio.1170070103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12496175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Marlene A. DeLuca (1936-1987). Marlene A. DeLuca(1936-1987)。

Journal of bioluminescence and chemiluminescence Pub Date : 1989-07-01

P E Stanley

引用次数: 0