A. Eissa, R. Korany, Rabia A. El Zlitne, Ayad Magdy, M. Sharaf, Awad Abdelbaky, Abeer E. Mahmoud, Rehab Abd ElMaged, Rehab A Qorany, A. Mohamed, Doaa Faisal, E. Hussein, Hatem H. Mahmoud, Hisham Elgazzar, Said El Behiri, Emad Afify, A. Prince, M. Attia
{"title":"Impact of Anisakis pegreffi Infection on Gonadal Health and Gonadosomatic Index of European Hake (Merluccius merluccius)","authors":"A. Eissa, R. Korany, Rabia A. El Zlitne, Ayad Magdy, M. Sharaf, Awad Abdelbaky, Abeer E. Mahmoud, Rehab Abd ElMaged, Rehab A Qorany, A. Mohamed, Doaa Faisal, E. Hussein, Hatem H. Mahmoud, Hisham Elgazzar, Said El Behiri, Emad Afify, A. Prince, M. Attia","doi":"10.21608/javs.2023.211605.1235","DOIUrl":"https://doi.org/10.21608/javs.2023.211605.1235","url":null,"abstract":"Parameters belonging to the physical status and gonadal size of certain fish provide crucial information for assessing both the productivity and fecundity of declining fish populations. These parameters are vulnerable to the negative impacts of disease agents such as internal or systemic parasites. Although parasitic diseases might influence these parameters, the literature investigating these pathophysiological alterations is scanty. Therefore, the current study represents one of the scarcest studies that document the possible link between parasitism, gonadal health, and the growth of European hake ( Merluccius merluccius ). Screening of imported European halves indicated a relatively high prevalence of Anisakis pegreffi Larvae 3 infestation, with an 80% prevalence rate, a mean intensity of 24.4, and a mean abundance of 19.5. However, the prevalence of Anisakid larvae infection in native fish was 36% with a mean intensity of 7.36 and mean abundance of 2.65.The current research revealed remarkable ovarian pathology that involved several forms of degenerative changes in ovarian tissues. Such gonadal pathologies were attributed to the damaging effect of the retrieved Anisakis pegreffi Larvae 3. Gonadosomatic index of both heavily infected imported / native hakes was relatively impacted by the progressive gonadal pathology resulting from Anisakis pegreffi L3 infection. Morphometric measurements of the gonads and body have revealed that, gonadosomatic index of both heavily infected imported / native hakes was relatively impacted by the progressive gonadal pathology resulting from Anisakis pegreffi L3 infection.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41655886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sub-acute Effects of α-Fe2O3 Nanoparticles on Some Biochemical Parameters in Mice","authors":"Nashwan Al-hamadany, Muna Azubaidy","doi":"10.21608/javs.2023.210749.1232","DOIUrl":"https://doi.org/10.21608/javs.2023.210749.1232","url":null,"abstract":"The goal of the study was to find out the toxic effect of daily treatment with α-Fe 2 O 3 nanoparticles for 14 and 28 days on some biochemical indicators in mice by measuring ferritin, transferrin, cholinesterase enzyme activity, the concentration of Caspase-3, and the concentration of glutathione and malondehyde in brain and liver tissues at doses of 75, 150 and 300 mg/kg. α-Fe2O3 nanoparticles at the dose of 75 mg/kg caused a significant decrease in the activity of the acetyl cholinesterase enzyme in the brain and liver after 14 days of treatment, compared with the control group and the doses of α-Fe 2 O 3 (75, 150, and 300 mg/kg) led to a significant decrease in the activity of the acetyl cholinesterase enzyme in the brain after 28 days of treatment. Fe 2 O 3 at a dose of 75 mg/kg on the 14th day and at a dose of 150 mg/kg on the 28 th day of treatment resulted in a significant increase in the caspase-3 enzyme in the brain compared to the control group. While the doses of 150 and 300 mg/kg for 28 days led to a significant increase in transferrin concentration compared with the control group, The α-Fe 2 O 3 nanoparticles at doses of 75, 150, and 300 mg/kg for 28 days caused a significant decrease in the concentration of glutathione in the liver and brain tissues compared with the control group, accompanied by a significant increase in the concentration of malondehyde in the brain and liver tissues at doses of 150 and 300 mg/kg of body weight. We conclude that repeated exposure to α - Fe 2 O 3 nanoparticles has toxic effects on vital organs such as the brain and liver, represented by a decrease in the concentration of acetylcholinesterase and its ability to induce oxidative stress through a decrease in glutathione concentration and an increase in malondehyde concentration in mice .","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46338443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Vancomycin-Resistant Enterococcus spp. Isolated From Mastitic cow’s milk","authors":"Marwa Abdali, A. Abdulrazzaq, R. Hamouda","doi":"10.21608/javs.2023.207770.1224","DOIUrl":"https://doi.org/10.21608/javs.2023.207770.1224","url":null,"abstract":"This study assessed the recrudescence of Enterococci in mastitis cow's milk and investigated their resistance to vancomycin. 300 samples were compiled from separate places and fields in Kirkuk, Iraq during the period from February to May, 2022. The samples were ioculated on the surface of bile esculin agar plates with sodium azide and then incubated at 37°C for 24–48 h. The characteristic pin-point colonies with a zone of black precipitate and morphologically resembling Enterococci spp. were further subjected to presumptive identification by Gram’ staining, catalase, and oxidase tests. All isolates were kept in BHIB with 30% glycerol at −70°C for further molecular detection. Enterococci isolates were tested for their susceptibility to different antibiotics by a disc diffusion technique. Based on the results of the sensitivity test, the ten isolates with the highest level of multiple resistances were selected from each of E. faecalis and E. faecium to examine the van A, van B genes by cPCR. The results of the bacteriological examination revealed that, 61 isolates (20.3%) of Enterococci According to phenotypic criteria; 42 isolates were E. faecalis and 19 were E. faecium . Add this to the confirmatory tests that revealed 25 isolates (8.3%) were E. faecalis and 10 isolates (3.3%) were E. faecium detected by PCR. Antimicrobial susceptibility tests indicated high levels of multi-resistant E. faecalis and E. faecium strains. Vancomycin-resistant strains were 40% and 30% for E. faecalis and E. faecium, respectively. The genetic sequences of E. faecalis and E. faecium isolates and phylogenetic trees were established and registered in GenBank-NCBI. They obtained accession numbers (OP566382) for E. faecium and (OP566380) for E. faecalis, which became references in Iraq and around the world.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46956004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mai A. Mohamed, Mai M. Zohdy, H. Aiedia, M. Emara, M. Abdallah, T. Nouman
{"title":"Effect of Casein-Based Edible Coats Embodying Sorbic and Ascorbic Acids on the organoleptic, Physicochemical and Microbiological Quality of Frozen Beef Kofta","authors":"Mai A. Mohamed, Mai M. Zohdy, H. Aiedia, M. Emara, M. Abdallah, T. Nouman","doi":"10.21608/javs.2023.209452.1229","DOIUrl":"https://doi.org/10.21608/javs.2023.209452.1229","url":null,"abstract":".","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41479626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Protective effect of Moringa on Salivary Glands of rats exposed to Electromagnetic Radiation Of Mobile Phone: A Histological study","authors":"H. Hussein, G. Taqa","doi":"10.21608/javs.2023.208828.1227","DOIUrl":"https://doi.org/10.21608/javs.2023.208828.1227","url":null,"abstract":"The growing widespread usage of cell phones has resulted in potential negative impacts on human health due to their use near the location of salivary glands, which might be affected by non-ionizing radiofrequency radiation emitted from cell phones. The purpose of the study was to investigate the effect of electromagnetic waves from cell phones on the salivary glands of male albino rats. Albino rats ( n = 42) were divided into 6 groups ( n = 7 per group). Group I, the control group, received normal saline. In Group II, animals received 200 mg/kg body weight of ethanolic extract of Moringa leaves orally by gavage for a period of 10 weeks with no cell phone radiation. In Group III, animals were exposed to the data mode for 2 hours per day for 10 consecutive weeks. In Group IV, animals were exposed to calling mode for 2 hours per day for successive 10 weeks. In Group V, animals were subjected to data mode for 2 hours per day and given 200 mg/kg of Moringa extract. Group VI: animals exposed to calling mode for 2 hours/day and receiving 200 mg/kg of Moringa extract orally for a period of 10 weeks. Salivary gland samples were prepared for histological analysis after the experiment. In histological sections, the cells lining mucous acini, granular convoluted tubules, and striated ducts displayed symptoms of atrophy, vacuolar degeneration, necrosis, and detachment from the basement membrane edoema. These outcomes were examined using a light microscope. As a result of cell phone electromagnetic radiation, male albino rats' salivary glands suffered pathological alterations.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47964935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Sheet, O. Sheet, Ahmed Alajmi, Omar A Al-Mahmood, Raad Alsanjary
{"title":"Isolation and Identification of Salmonella from Chickens Prepared for Slaughter in the State of Kuwait","authors":"O. Sheet, O. Sheet, Ahmed Alajmi, Omar A Al-Mahmood, Raad Alsanjary","doi":"10.21608/javs.2023.208149.1226","DOIUrl":"https://doi.org/10.21608/javs.2023.208149.1226","url":null,"abstract":"Salmonella enterica serovar Enteritidis throughout the world, is frequently linked to food-borne illness. Products made from poultry are important sources of transmission. Therefore, this study aimed to identify the incidence of Salmonella species in Kuwait's broiler flocks and determine which antibiotics are the most effective against the various Salmonella serotypes. A total of 2064 chicken samples (liver, intestine, and caecum) were collected from dead carcasses raised ten broiler flocks, between January and December 2017. The results revealed that S. enterica were found in the chicken older than 7 days old even though they did not have any signs of a clinical illness. It means that S. enterica can enter the human food supply through slaughterhouses and the contaminate carcasses. There was significant difference between the rate of Salmonella isolation and seasons, where the winter season had higher rate of Salmonella isolation compared to the other seasons. Salmonella Enteritidis and S. typhimurium were the most frequently isolated serotypes. Antimicrobials susceptibility testing showed that 88% to 60% of the isolates were sensitive to Amoxicillin Clavulanic acid. However, most of Salmonella isolates revealed high resistance to Cefotaxime, Ampicillin, Amoxicillin, Sulfamethoxazole trimethoprim followed by Gentamycin, Spiramycin, Doxycycline and Chloramphenicol. The study highlights the implementation of unique biosecurity and biocontrol strategies for Salmonella management that can prevent the negative effects of antibiotics and can make the environment and foods derived from animals safe. ـــــــــــــــــــــــــــــــــــــــــ","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68514009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EFFECTS OF MARBOFLOXACIN AND KETOPROFEN ON SOME BIOCHEMICAL AND COAGULATION PARAMETERS IN CALVES","authors":"M. Ural, K. Uney","doi":"10.21608/javs.2023.208022.1225","DOIUrl":"https://doi.org/10.21608/javs.2023.208022.1225","url":null,"abstract":"Marbofloxacin (MBX) is a 3rd generation fluoroquinolone specifically developed for animal health and is an approved antimicrobial agent for the cure of mastitis and respiratory diseases in cattle and swine. Ketoprofen (KTP) is a nonsteroidal anti-inflammatory drugs (NSAID) belonging to the aryl propionic acid group and is used in musculoskeletal inflammation and pain, abdominal pain and other inflammatory circumstances. The objective of this study is to investigate the impact of simultaneous administration of MBX and KTP on the coagulation and biochemical parameters in calves. In the study, 18 clinically healthy calves were randomly separated into 3 groups of six animals each. The first group of calves received a single dose of MBX at 8 mg/kg, the second group received MBX at 8 mg/kg along with concurrent KTP at 3 mg/kg and the third group received only KTP at 3 mg/kg dose. The drugs were administered via intramuscular (IM) injection in the neck region. Biochemical and coagulation parameters were evaluated using an automated analyzer and coagulation analyzer, respectively. The IM injection of MBX into calves significantly increased creatine kinase (CK) and lactate dehydrogenase (LDH) values, which may be related to muscle damage. This may limit the administration via IM route of MBX to calves. KTP increased the prothrombin time (PT) and activated partial thromboplastin time (aPTT) values but decreased the fibrinogen value. It can be stated that in alone MBX, and KTP administration and their combination, further investigations are required to determine the safety of drugs after repeated administrations and other administration routes in calves.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47471817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Efficacy of Carbomer as a Stabilizer for lyophilized attenuated Peste des Petits Ruminants (PPR) vaccine","authors":"A. Mohamed","doi":"10.21608/javs.2023.204533.1219","DOIUrl":"https://doi.org/10.21608/javs.2023.204533.1219","url":null,"abstract":"Peste des petits ruminants (PPR) is a category of acute fulminating infectious viral disease, affecting seriously sheep and goats. It is caused by PPRV that classified within a Morbillivirus; family Paramixoviridae. Vaccination is the cornerstone to protect sheep and goats against such diseases. The present work aims to provide a highly potent attenuated lyophilized PPR vaccine where three formulae were prepared including formula (1) stabilized with 1% carbomer and 2% peptone; formula (2) stabilized with 0.5% carbomer and formula (3) stabilized with 2% peptone; 10% sucrose and 0.27% dihydrogen orthophosphate. The three lyophilized PPR vaccine formulae had compact similar cacks without different shape appearances. Also, the three formulae were found to be free from foreign contaminants (aerobic and anaerobic bacteria; fungi and mycoplasma) having no significant difference in the virus titer pre-and post-lyophilization. Reduction in the virus titer post lyophilization was 0.25, 0.5 and 0.5 log10 TCID 50 /ml in relation to formula 1; 2 and 3 respectively. Although formula (2) induced the peak PPR serum neutralizing antibody titer earlier (128 by the 3 rd week) than the other 2 formulae (128 by the 4 th week); the potency test revealed that all vaccine formulae were potently inducing high protective PPR immune levels in vaccinated sheep up to 6 months post-vaccination. So, it could be suggested that carbomer accelerate the time to reach the peak of PPR antibody titers in vaccinated sheep. ـــــــــــــــــــــــــــــــــــــــــ","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48763861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of Amitriptyline and Ashwagandha on the Oxidative State and Acetylcholine Esterase Enzyme Activities in Rats","authors":"Ismael Idrees, G. Taqa, Saba ALtaaye","doi":"10.21608/javs.2023.191488.1214","DOIUrl":"https://doi.org/10.21608/javs.2023.191488.1214","url":null,"abstract":"","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49282668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Marek's disease salience in domestic poultry ,gross and a Histopathological study at Mosul city","authors":"H. Al-sabawy","doi":"10.21608/javs.2023.190440.1213","DOIUrl":"https://doi.org/10.21608/javs.2023.190440.1213","url":null,"abstract":"","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49527641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}