Journal of Applied Animal Research最新文献

{"title":"Analysis of tissue lesions and expression of HIF-1α and VEGF in broiler ascites syndrome","authors":"Tao Li, Kai Li, Zhichao Wang, Jiang He, Ruihuan Gao, Changqing Shi, Meng Qi, Aikebaier Reheman, Xinwei Feng","doi":"10.1080/09712119.2025.2604962","DOIUrl":"https://doi.org/10.1080/09712119.2025.2604962","url":null,"abstract":"Broiler ascites syndrome (BAS), a hypoxia-driven metabolic disorder, causes significant economic losses in poultry production. This study aimed to evaluate the pathological changes in the lungs and liver of broiler chickens and the expression of HIF-1α and VEGF. HE, Masson and Van Gieson staining were used to observe histopathological changes in the lungs and liver, and molecular biology methods such as qPCR, Western blotting and immunohistochemistry were used to detect the expression of HIF-1α and VEGF in the lungs and liver. The histopathological results revealed significant thickening and remodelling of the pulmonary artery wall in BAS broiler chickens; the boundaries of the vascular intima, media and adventitia were unclear; and there was a significant increase in the collagen fibre content around the arteries. Additionally, the hepatic cords were loosely arranged liver cords. The molecular biology results revealed that both the mRNA and protein expression levels of HIF-1α and VEGF were significantly increased (p < 0.05) in the lungs and liver of AS broiler chickens. These findings establish an association between HIF-1α/VEGF dysregulation and BAS pathogenesis, providing a foundation for mechanistic investigation.","PeriodicalId":15030,"journal":{"name":"Journal of Applied Animal Research","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breeders at 46 weeks of age supplemented with 1,25(OH) ₂ D ₃ -G produce heavier chicks at hatch, with improved bone health and immunity at 21 days of age","authors":"Thiago dos Santos Andrade, Nilton Rohloff, Barbara Amanda Bebber, Matheus Leandro Reis Maia, Mario Pereira, José Geraldo de Vargas, Bruno Serpa Vieira, Paulo Levi de Oliveira Carvalho, Cínthia Eyng, Ricardo Vianna Nunes","doi":"10.1080/09712119.2025.2591649","DOIUrl":"https://doi.org/10.1080/09712119.2025.2591649","url":null,"abstract":"This study evaluated the effects of 1,25-Dihydroxycholecalciferol glycoside (1,25(OH)2D3-G) on performance, intestinal histomorphometry, blood biochemistry, bone health, and gene expression in broilers from breeders. A total of 1,152 one-day-old male Ross 308 AP chicks were assigned to a completely randomized 2 × 3 factorial design. One factor was the supplementation (0 or 100 mg kg⁻¹ ) of 1,25(OH)2D3-G in breeders' diets from 21 to 46 weeks. The other was its supplementation (0, 50, 100 mg kg⁻¹ ) in broiler diets from 1 to 21 days. The experiment consisted of six treatments, eight replications, and 24 birds per experimental unit. Supplementation of 1,25(OH)2D3-G in breeders increased chick hatch weight and improved bone health in broilers at 21 days, with higher calcium and phosphorus deposition and greater tibia strength. It also increased calbindin D28K, interleukin 10, and interleukin 1β in the jejunum. Broilers supplemented with 50 mg kg⁻¹ showed greater phosphorus deposition in the tibia, while those receiving 50 or 100 mg kg⁻¹ had higher interleukin 10 when sourced from supplemented breeders. This study concludes that supplementation with 100 mg kg⁻¹ of 1,25(OH)2D3-G in breeders produces heavier chicks at hatch and enhances bone health and immunity in broilers at 21 days.","PeriodicalId":15030,"journal":{"name":"Journal of Applied Animal Research","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147911974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of low-protein diets on broiler nutrition, production sustainability, gene expression, meat quality and greenhouse gas emissions","authors":"Ahmad Salahi, Mohammad Hossein Shahir, Youssef A. Attia, Khaled Nasr El-din Fahmy, Fulvia Bovera, Vincenzo Tufarelli","doi":"10.1080/09712119.2025.2473419","DOIUrl":"https://doi.org/10.1080/09712119.2025.2473419","url":null,"abstract":"This review explores the effects of low-crude protein diets (LCPD) on broiler chicken production, focusing on sustainability, gene expression, meat quality and environmental impacts. LCPD supplemented with amino acids, enzymes, and probiotics has been shown to influence protein utilization efficiency, meat quality, environmental pollution, and bird welfare. However, gaps exist in the understanding of the relationship between LCPD and immunity, footpad lesions, gut microbiota, economic aspects, and amino acid sparing. LCPD and plant-based protein sources significantly reduced C. perfringens in ileum and cecum, while increasing the abundance Lactobacillaceae. Amino acid digestibility increased in ileum and jejunum with LCPD, and fortification with specific amino acids, while maintaining the non-essential to essential amino acid ratio (NEAAs/ EAAs) at 47:53, resulted in optimal performance. In addition, the reduction of CP to 15% decreased tibia length, calcium retention, and bone mass accumulation. Additionally, 20% excessive total amino acids (TAAs) significantly reduced tibial mineralization. Further research is needed to optimize LCPD formulations and address potential barriers to their implementation in the poultry industry. The available literature emphasizes the effects of LCPD supplemented with amino acids, enzymes, and probiotics. on broiler performance, protein utilization efficiency, meat quality, environmental pollution, green gas emissions, health, and bird welfare.","PeriodicalId":15030,"journal":{"name":"Journal of Applied Animal Research","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.tandfonline.com/doi/pdf/10.1080/09712119.2025.2473419?needAccess=true","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147881061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiRNA-455-5p regulates the growth and development of adipose tissue by targeting <i>IGF-1R</i> gene","authors":"Min Wu, Lingli Feng, Yubin Wang, Kun Zhang, Ganqiu Lan, Jing Liang","doi":"10.1080/09712119.2024.2399510","DOIUrl":"https://doi.org/10.1080/09712119.2024.2399510","url":null,"abstract":"Adipose tissue is an important factor that influences porcine quality, and adipose tissue is also an important endocrine regulator of metabolic homeostasis. Adipose tissue development involves a series of highly synergistic processes, including clone amplification, proliferation arrest and terminal differentiation. In recent years, microRNAs (miRNAs) are a class of non-coding endogenous RNA molecules that have been considered as a class of novel regulators of adipogenesis. In this study, we used the dorsal subcutaneous adipose tissue of Guangxi Bama mini-pigs and Landrace pigs as experimental materials to study the molecular mechanism of adipogenic differentiation of preadipocytes, and the regulatory mechanism of miRNA-455-5p and its target gene IGF-1R involved in the development of adipose tissue. These findings may improve our understanding of mechanism of the molecular regulation of miRNA-455-5p and IGF-1R in pigs, and provide a theoretical basis for increasing pork production.TEASER TEXT The miRNA-455-5p was involved in the negative regulation of adipose tissue development by inhibiting the expression of its target gene IGF-1R.ABSTRACT Adipose tissue is an organ with metabolic, endocrine and immune functions, affecting the life span of animals and their susceptibility to diseases. Previous studies have reported the relationship between many miRNAs and the development of adipose tissue. However, the regulation of miR-455-5p on the development of porcine adipose has not been reported. In this study, we used the dorsal subcutaneous adipose tissue of Guangxi Bama mini-pigs and Landrace pigs as experimental materials to study the regulatory mechanism of miRNA-455-5p and its target gene IGF-1R involved in the development of adipose tissue. Bioinformatic analysis with Targetscan predicted that IGF-1R might be a target gene of miRNA-455-5p. Moreover, mouse 3T3-L1 preadipocytes were transfected with chemically modified mimics and inhibitors to observe the expression of miR-455-5p and IGF-1R genes from 0 to 8 days after transfection. And the expression of adipogenic marker genes PPARγ and C/EBPα was examined. Additionally, the miRNA-455-5p may be involved in the negative regulation of adipose tissue development by inhibiting the expression of its target gene IGF-1R. These results provide a theoretical basis for increasing pork production and provide a new potential target for the treatment of obesity and type 2 diabetes in humans.","PeriodicalId":15030,"journal":{"name":"Journal of Applied Animal Research","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147908342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production system analysis and breeding practices of indigenous goats in North Wollo zone, Amhara region, Ethiopia","authors":"Yeshareg Taye, Mengistie Taye, Kefyalew Alemayehu, Zeleke Tesema","doi":"10.1080/09712119.2024.2385042","DOIUrl":"https://doi.org/10.1080/09712119.2024.2385042","url":null,"abstract":"This study describes the goat production system and breeding practices in selected districts of North Wollo zone, Ethiopia. Data were collected from 180 respondents through a semi-structured questi...","PeriodicalId":15030,"journal":{"name":"Journal of Applied Animal Research","volume":"16 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142260888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary fat sources on growth performance, lipid metabolism and intestinal health of weaned piglets","authors":"Fawen Dai, Shiwei Zhao, Xia Huang, Hang Yu, Tao Lin, Muqu Jin, Jing Ma, Yan Chen, Chaohao Li","doi":"10.1080/09712119.2024.2384417","DOIUrl":"https://doi.org/10.1080/09712119.2024.2384417","url":null,"abstract":"We investigated the effect of different fat sources on growth performance and intestinal health in weaned piglets. A total of 72 21-day-old weaned piglets were divided into 3 groups the CON group w...","PeriodicalId":15030,"journal":{"name":"Journal of Applied Animal Research","volume":"363 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141880764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of Aronia melanocarpa (AM) dietary supplementation on production performance, meat and egg quality, yolk volatile substances and antioxidant capacity of laying hens","authors":"Bo Jing, Haoyuan Wu, Zhenhua Liu, Hongmei Shang, Yuting Li, Zhouyu Jin, Hui Song","doi":"10.1080/09712119.2024.2381729","DOIUrl":"https://doi.org/10.1080/09712119.2024.2381729","url":null,"abstract":"The effects of Aronia melanocarpa (AM) dietary supplementation on production performance, meat and egg quality, yolk volatile substances and antioxidant capacity of laying hens. A total of 480 Roma...","PeriodicalId":15030,"journal":{"name":"Journal of Applied Animal Research","volume":"49 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141946004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on underestimated; but pertinent challenges of cattle breeding: the Ethiopia perspective","authors":"Tassew Mohammed Ali","doi":"10.1080/09712119.2024.2375246","DOIUrl":"https://doi.org/10.1080/09712119.2024.2375246","url":null,"abstract":"The review aimed to explore and document the underestimated; but pertinent challenges of cattle breeding in order to get due attention from the scientific communities, policy makers, and farmers. E...","PeriodicalId":15030,"journal":{"name":"Journal of Applied Animal Research","volume":"42 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141614024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On-farm performance evaluation of indigenous sheep in the Gedeo zone, Southern Ethiopia","authors":"Debir Legesse, Amelmal Alemayehu","doi":"10.1080/09712119.2024.2375245","DOIUrl":"https://doi.org/10.1080/09712119.2024.2375245","url":null,"abstract":"This study was conducted to identify the productive and reproductive performance of sheep in the Gedeo zone. Flock of 50 households was randomly selected for a household survey. A total of 189 lamb...","PeriodicalId":15030,"journal":{"name":"Journal of Applied Animal Research","volume":"22 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141614033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diluted seawater decreased weight gain and altered blood biochemical parameters in Bach Thao goats","authors":"Thiet Nguyen, Ngu Nguyen Trong, Narongsak Chaiyabutr, Sumpun Thammacharoen","doi":"10.1080/09712119.2024.2371123","DOIUrl":"https://doi.org/10.1080/09712119.2024.2371123","url":null,"abstract":"This study aimed to evaluate the effect of saline water (SW) on physiological responses, blood biochemical parameters and body weight changes in Bach Thao goats. The study was designed completely r...","PeriodicalId":15030,"journal":{"name":"Journal of Applied Animal Research","volume":"15 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141614025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}