Journal of Advances in Biology & Biotechnology最新文献

{"title":"Compositional Assessment of Selected Plant-based Substrates for Biogas Production","authors":"U. Nicholas, Nwachukwu, Augusta Añuli, Ogbulie Tochukwu Ekwutosi, Anyalogbu Ernest Anayochukwu","doi":"10.9734/JABB/2021/V24I330202","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I330202","url":null,"abstract":"Enormous quantities of plant biomass are generated annually, as agricultural wastes. Lignocellulose is the main structural constituent of plants and represents the primary source of renewable organic matter on earth. This study was carried out to evaluate the lignocellulose composition, proximate and selected physicochemical characteristics of some selected plant-based substrates for biogas production. The substrates were: Corn cobs, Rice straw and Water hyacinth (Eichhorniacrassipes). They were collected, cut, dried for 72 hours at 32C, milled and subjected to hemicellulose, lignin and cellulose compositional analyses, using the standard Sox let extraction method. Standard methods were employed for proximate and physicochemical analyses. Results of the compositional evaluation showed that corn cob has the highest percentages of cellulose (42.0%), while extractives content was least (2.18%) in Rice straw. For the proximate analysis, the percentage carbohydrates (24.22) and ash (24.40) were highest in rice straw, while fat content had the least values of 0.65% recorded in corn cobs. The results of the physicochemical analysis showed that Rice straw had the highest values of TS (94.55%) and phosphorus (928.57mg/kg), Corn cob had the highest TVS Original Research Article Ugwu et al.; JABB, 24(3): 1-6, 2021; Article no.JABB.67948 2 (85.53%) and organic carbon (50.46%) while Water hyacinth recorded the highest Nitrogen content (2.33%). They are good substrates for energy generation, and lignocellulosic biomass holds a huge potential to meet the current energy demand of the modern world. The knowledge of the lignocellulosic composition of the biomass would help in choosing appropriate pretreatment measures to achieve better hydrolysis which would translate to higher biogas yield.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"30 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2021-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84645356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological Characteristics of Scutellonema bradys Populations Responsible for Yam Dry Rot in Côte d’Ivoire","authors":"Yadom Y. F. R. Kouakou, K. Kra, Toualy Marie Noël Yeyeh, H. Diallo","doi":"10.9734/JABB/2021/V24I230201","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I230201","url":null,"abstract":"The great diversity of agroecological factors that cover the yam production area in Côte d’Ivoire can be a source of morphological variability within Scutellonema bradys species, responsible for yam dry rot. This study aims at identifying the morphological group(s) of S. bradys which infect(s) yam Dioscorea cayenensis-rotundata. Two hundred ten yam tubers for sale in food markets in the Autonomous District of Abidjan were sampled from traders. After extraction and identification, a morphological characterization was carried out on S. bradys populations using morphological descriptors. Yam tubers sampled from food markets came from three agroecological zones namely dry tropical savannah, semi-deciduous dense forest and transitional forest areas. Morphometric variables and ratios used individually did not help identify morphological groups. Principal component analysis, however, performed with the most discriminating variables and ratios revealed three morphological groups named \"large\", \"medium\" and \"small\" in males and females and confirmed by the agglomerative hierarchical clustering. Each group consisted of a rate greater than 48% of individuals from a given agroecological zone. Three morphological groups of S. bradys are associated with yam dry rot in Côte d’Ivoire. Pathogenic and molecular characterization of the morphological groups would be necessary with a view to researching control methods. Original Research Article Kouakou et al.; JABB, 24(2): 43-59, 2021; Article no.JABB.67637 44","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"46 1","pages":"43-59"},"PeriodicalIF":0.0,"publicationDate":"2021-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86934218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorative effect of vitamin C on cypermethrin-induced hepatotoxicity and renal malfunction of adult male rats.","authors":"N. Kheirallah, H. K. Hussein, Horeya Said Abd El Gawad, B. Youssef","doi":"10.24297/JAB.V14I.9007","DOIUrl":"https://doi.org/10.24297/JAB.V14I.9007","url":null,"abstract":"Cypermethrin is a widely used insecticide belongs to type II pyrethroids. It is highly used in developing countries to control many species of insects as it plays an important role in pest control. Vitamin C is an important intracellular antioxidant against insecticides. The aim of the present study is to analyze the toxic effects of cypermethrin (CYP) and the positive effect of vitamin C (VC) with graded doses (0 CYP, 200 VC, 12 CYP and 12 CYP+200 VC mg/kg body weight of male rats/day) for 30 days on liver and kidney functions. Moreover, antioxidant enzymes, oxidative stress markers, glycogen and glucose levels and also serum lipid profile were studied. Also, this study aimed to evaluate the possible protective role of vitamin C against cypermethrin toxicity","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75691288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on Interactive Effects of Different Levels of Lead and Mercury on Nitrogen Fixation of Some Diazotrophs","authors":"Kenechukwu U. Aroh, J. Udensi","doi":"10.9734/JABB/2021/V24I230200","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I230200","url":null,"abstract":"Researchers have studied the effects of addition of metal elements in combination with nitrogen fixing organisms as inoculants on the plants (growth) predominantly in legumes, however there is a major gap because responses and effects of these proposed micronutrients on the nitrogen fixation activity of these microbes both free living and symbiotic remains sketchy at best. Therefore, the effect of supplementation of lead and Mercury (bioaugmentation) on the nitrogen fixation potential of two (2) diazotrophs was evaluated in this study. Aims: To evaluate the interactive effects of different levels of Lead and Mercury on Nitrogen fixation of both Rhizobium and Xanthobacter spp in-vitro. Place and Duration of Study: Sample organisms where collected from Groundnut rhizospheric soil of a farm in Cross River state, Nigeria. The microorganism isolation and nitrogen fixation analysis was further carried out at MacCliff General services Laboratory, Owerri, Nigeria for a duration of 3 months. Study Design: The interactive plots serve to show the effect of one variable (lead) on the value of mercury (the other) and is derived by selecting high and low values for lead (Pb) and entering them into the equation along with the range of values for Mercury (Hg). The values of independent Original Research Article Aroh and Udensi; JABB, 24(2): 34-42, 2021; Article no.JABB.67623 35 variables (lead and mercury levels) used in the plots were selected by observing the highest concentration (+1) and lowest concentration (-1) values which are able to support nitrogen fixation independently in Rhizobium and Xanthobacter. Methodology: The soil samples were collected from groundnut rhizosphere at a 20 cm depth using sterile soil corer (sterilized with 95% ethanol) and matured Groundnut plants were uprooted with care. From these samples, both Rhizobium and Xanthobacter spp were isolated. The isolated organisms were re-vitalized in Jensen’s nitrogen free broth and standardized to 0.5 McFarland standards. To determine nitrogen fixation, the broth cultures were examined for nitrate nitrogen (NO3-N) and amino nitrogen (Amino-N) levels after ten days of the experiment under continuous airflow using the Jensen’s nitrogen free broth containing the metal salts, Mercury (II) chloride HgCl2 and Lead (II) acetate trihydrate Pb (CH3COO)2.3H2O). Nitrate nitrogen and amino Nitrogen was obtained using cataldo and ninhydrins methods respectively. The data obtained was made in triplicates and reported as mean values. Interactive effect plots and statistical analysis were done using Minitab 17 software at 5% level of significance (p<0.05). Results: The main effect plots illustrate that to maximize nitrogen fixation in Xanthobacter sp through the utilization of the selected metals as micronutrient, we should use lead at 6.25 mg/L and mercury at 25mg/L yielding 0.508 mg/L for nitrogen fixation response. The plot also suggests that if lead metals are used at a higher concentration than stated ni","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"41 1","pages":"34-42"},"PeriodicalIF":0.0,"publicationDate":"2021-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91342534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viability and in vivo Hypocholesterolemic Effect of Lactobacillus plantarum 29V in Local Honey","authors":"Ulrich Landry Kamdem Bemmo, Chancel Hector Momo Kenfack, J. Bindzi, R. Barry, François Zambou Ngoufack","doi":"10.9734/JABB/2021/V24I230199","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I230199","url":null,"abstract":"The conservation of probiotic products requires low temperatures and suitable equipment that are less available in developing countries. The challenge today is to find a local food matrix that can also carry probiotics (microorganisms with benefits for consumers) in the gastro-intestinal tract. The study mainly focus in the current research was to study the use of honey as a food matrix to carry probiotics in treating the cardiovascular disease, hypercholestreolemia. Thus, this study aimed to assess the viability of Lactobacillus plantarum 29V and its in vivo hypocholesterolemic properties when contained in honey. The strain L. plantarum 29V was added in pasteurized honey and was studied its viability in honey and its impact on the physicochemical parameters of honey. For in vivo studies, 0.5 mL of the pasteurized honey containing approximately 10 8 CFU/mL of L. plantarum 29V were administered to rats fed on a cholesterol-enriched diet (control diet+ pure Original Research Article Bemmo et al.; JABB, 24(2): 24-33, 2021; Article no.JABB.67117 25 cholesterol solution (0.04 g/mL) per day per rat) using a feeding syringe; the treatment lasted 4 weeks. Serum lipids were analyzed during the experiment. The results have shown that the probiotic strain L. plantarum 29V can survive in honey for 28 days without affecting the honey’s qualities. Even present in honey, this strain continues to lower serum total cholesterol, (VLDL +LDL)cholesterol and triglycerides levels of hypercholesterolemic rats. In addition, HDL-cholesterol levels significantly increased, and the atherosclerosis index was significantly lowered. The present study revealed that honey could be used as a food matrix to carry the probiotic Lactobacillus plantarum 29V strain very well into the gastro-intestinal tract. Hence, a probiotic formulation made of pasteurized honey and L. plantarum 29V would be used to treat or prevent hypercholesterolemia if these effects are confirmed in Human beings.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"54 2 1","pages":"24-33"},"PeriodicalIF":0.0,"publicationDate":"2021-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77204765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Nutrient, Mineral and Anti-Nutrient Studies of Two Indigenous South African Spices","authors":"A. Asowata-Ayodele, G. Otunola, A. Afolayan","doi":"10.9734/JABB/2021/V24I230198","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I230198","url":null,"abstract":"The two South African spices named Lippia javanica (Izinziniba) and Foeniculum vulgare (Imbambosi) were studied for its proximate principles, minerals, vitamins and anti-nutrients properties. The experiment shows that L. javanica had higher figure in its lipids, fibres and carbohydrates contents. This implies that L. javanica may help the body to maintain an internal distention for proper peristaltic movement of the intestinal tract than F. vulgare will do. While, the protein, ash, moisture contents were more in F. vulgare, this may be the reason, more elemental composition were observed in it because the level of ash, moisture and protein contents determines its minerals or inorganic components. Elements such as Nitrogen (N), Potassium (K), Magnesium (Mg) and Sodium (Na) were observed to be more to mentioned few, the Ca/P levels of both plants was observed to be more than 0.5 which is the minimum ratio required for favourable calcium absorption in the intestine for bone formation. Likewise both plants shows no significant differences in its anti-nutrients levels such as the phytate, oxalate and tannin contents, but L. javanica had more of Vitamin A and E levels, compared to F. vulgare with better Vitamin C, Cyanide and Saponin contents. In general, spices have been reported to contribute to the average Original Research Article Asowata-Ayodele et al.; JABB, 24(2): 15-23, 2021; Article no.JABB.67397 16 requirement for different nutrients. Polyphenols such as tannins have anticancer properties, so beverages such as green tea that contain large amount of these compounds might also be good for maintenance of human health despite its anti-nutrient properties. Comparatively, L. javanica has a better nutrient composition than F. vulgare.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"7 1","pages":"15-23"},"PeriodicalIF":0.0,"publicationDate":"2021-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87400371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutics Activities of Amazonian Plant Himatanthus sucuuba (Spruce ex Müll. Arg.) Woodson (Apocynaceae): A Review","authors":"Milene Lopes da Silva, Márcia Pinheiro da Silva, José Dobles Dias dos Reis Júnior, C. Lima, Anderson de Oliveira Souza","doi":"10.9734/JABB/2021/V24I230197","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I230197","url":null,"abstract":"Several compounds extracted from medicinal plants and their active ingredients can relieve symptoms and even cure diseases, although they occasionally have adverse effects. The knowledge of their properties has been transmitted over the centuries within and among human communities. Himatanthus sucuuba is an Amazon plant that has its value attributed to the different herbal impacts popularly reported. This review presents significant biological activities such as antibacterial, antifungal, anthelmintic, antileishmanial, anti-inflammatory, analgesic, antidepressant, immunoregulatory, cytotoxic, and genotoxic. Thus, provides a synopsis of the literature exploring the extracts of H. sucuuba to the Amazon region that could efficiently prevent pathologies associated with leishmaniosis, infection for bacteria or fungus, depression, oxidative stress, and cytotoxicity or genotoxicity. Review Article Silva et al.; JABB, 24(2): 1-14, 2021; Article no.JABB.66598 2","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"1 1","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2021-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89872339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Water Hyacinths (Eichhornia crassipes) – Application for Secondary Wastewater Treatment and Biomass Production","authors":"K. Dölle, Q. Wang, J. Tong","doi":"10.9734/JABB/2021/V24I130196","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I130196","url":null,"abstract":"Clean water is one of the most significant challenges for our society. Efficient reuse of effluent water after treatment can becomes an effective solution to the shortage of water resources. The focus of this study is to investigate the use of Eichhornia crassipes plants for post treatment of clarified municipal residential sewage under natural conditions using a small pilot Laboratory Water Hyacinth Clarifier system. Twelve Eichhornia crassipes plants are used to investigate total phosphorus and ammonia nitrogen removal during a 20-day study period under various retention rates. The biomass gain of the Eichhornia crassipes plants was 2.4-fold from the initial weight of 1556.5 g to 3676.7 g. Total phosphorous reduction of 10.64%, 11.83%, 20.93%, 41.66%, 67.12%, and 40.13% for the 1.5, 9.0, 12.0, 24.0, 48.0 h, and 120.0-hour retention times respectively. Ammonia nitrogen removal was between 35.71%, 33.33% for the 1.50 and 9.0-hour retention time and 42.85% for the 12.0 and 24.0-hour retention time. A reduction of 71.43% resulted for the 48.0-hour retention time and an 85.71% reduction for the 120.0-hour retention time. Overall retention time of 24.0 h, 48.0 h and 120 h tend to give best removal rates for both total phosphorus and ammonia nitrogen removal. Factors Original Research Article Dölle et al.; JABB, 24(1): 52-61, 2021; Article no.JABB.66324 53 such as climate, contaminant concentration, retention rate, and weather conditions play an important role for the application of Eichhornia crassipes in a tertiary treatment sequence of MRS.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"38 1","pages":"52-61"},"PeriodicalIF":0.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87799828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a Fraction of the Methanol Extract of the Seeds of Abrus Precatorius on Malondialdehyde and Antioxidant Levels of Alloxan-Induced Diabetic Rats","authors":"E. L. Iloanya, O. Nwodo, O. Ezeigwe, Ogochukwu Prince Alaebo, Nnanyelugo Benneth Enemchukwu, Okwuchukwu Bibian Aziagba, Ifeanyi Sunday Okpani","doi":"10.9734/JABB/2021/V24I130195","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I130195","url":null,"abstract":"Introduction: The aim of this study is to evaluate the effect of fraction 2 (F2) of methanol extract of the seeds of Abrus precatorius on malondialdehyde and antioxidant level of alloxan-induced diabetic Wistar albino rats. Original Research Article Iloanya et al.; JABB, 24(1): 35-51, 2021; Article no.JABB.66274 36 Materials and Methods: The methanol extract of the seeds of A. precatorius Linn Fabaceae was fractionated by Sephadex G15. Diabetes was induced by a single intraperitoneal administration of alloxan at a dose of 150 mg/kg bodyweight. The phytochemical analysis and the biochemical parameters were investigated using standard diagnostic methods. Results: Both qualitative and quantitative analysis of F2 revealed the presence of high amounts of alkaloids (2000 ± 80 mg/100 g), flavonoids (158 ± 17.6 mg/100 g) and tannins (258 ± 45 mg/100 g) but low concentration of saponins (18.3 ± 2.43 mg/100 g). The malondialdehyde (MDA) level of all the test groups and the group treated with the standard drug decreased significantly (p<0.05) compared with that of untreated diabetic group. However, the catalase activity in all the test groups significantly increased (p<0.05) while the activity of superoxide dismutase increased significantly (p<0.05) in groups administered 20mg/kg of the fraction and the group pre-treated with 10mg/kg of the fraction compared with the diabetic untreated group. Similarly, the result revealed a significant increase (p<0.05) in non-enzymatic antioxidants such as reduced glutathione and vitamin E of the rats treated with graded doses of F2 while vitamin C showed significant (p<0.05) increase in normal control groups and pre-treated groups compared with the diabetic untreated. Studies on membrane stabilization using hypotonicity-induced red blood cell haemolysis revealed that the F2 of the methanol extract of the seeds of A. precatorius Linn inhibited haemolysis in a dose-dependent manner. Conclusion: The results obtained from this study revealed that the F2 fraction of the methanol extract of A. precatorius contain important phytochemicals found in the Fraction 2 (F2) however showed improvement in antioxidant defence as well as has a stabilizing effect on the membrane.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"45 1","pages":"35-51"},"PeriodicalIF":0.0,"publicationDate":"2021-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75815565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Direct and Indirect Sunlight on Polythene Packs, Sensory, Microbial and Chemical Properties of Sachet Water","authors":"O. Adedire, A. Atere, W. Ogundipe, A. Farinu","doi":"10.9734/JABB/2021/V24I130194","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I130194","url":null,"abstract":"The use of Polyethylene or Polyethylene terephthalate (PE/PET) packaging bags for water in Nigeria has great marketing appeal to consumers. However, poor storage and display techniques could subject these products to microbial, physical and chemical deterioration. Sachet water samples commonly taken by staff and students of the Federal College of Agriculture, Ibadan were subjected to different storage conditions for three and five days. Physicochemical parameters of each water sample were determined through equilibrated devices and titrimetric or colorimetric assays, while aerobic bacteria were isolated through pour-plate method in Nutrient Agar. Exposure of sachet water to sunlight had significant effects on their physical, chemical and bacteriological properties. Highest chloride, calcium, alkalinity, hardness, sulphate and total dissolved solids (13.04 mg/L, 123.53 mg/L, 9.08 mg/L, 33.00 mg/L, 27.59 mg/L and 78.33 mg/L, respectively) were recorded in water samples exposed to indirect sunlight for 5 days. Samples stored at room temperature had the best physicochemical properties. Exposure to sunlight reduced the colony forming units of aerobic bacteria in all the water samples. Lowest bacterial count (0.33 × 10 3 CFU/mL) was observed in water sample exposed to sunlight for 5 days, while the highest count Original Research Article Adedire et al.; JABB, 24(1): 25-34, 2021; Article no.JABB.66080 26 (55.84 CFU/mL) was recorded in samples stored at room temperature. Sensory scores of water samples ranged between 8.33 and 10.00; however, these sensory properties were not significantly affected by their exposure to sunlight for up to 5 days. Sunlight exposure negatively affected the inner surface feel of water packs, sachets appeared slimy after direct and indirect exposure. The physicochemical and microbial changes observed in sunlight-exposed sachet water samples did not influence their organoleptic acceptability. Consequently, in addition to taste and biochemical analysis, the feel of water sachets could be an indication of structural disintegration and water contamination. In order to maintain the integrity of sachet water, products should be hygienically prepared and prevented from sunlight exposure during transportation and storage.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"3 1","pages":"25-34"},"PeriodicalIF":0.0,"publicationDate":"2021-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82563412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}