Journal of Applied and Natural Science最新文献_第4页

Detection of Bacteroides fragilis LuxR gene, involved in quorum sensing, among colitis patients in Mosul, Iraq 在伊拉克摩苏尔的结肠炎患者中检测到参与定量感应的脆弱拟杆菌 LuxR 基因

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.4716

A. Sulaiman, Assala Saad

{"title":"Detection of Bacteroides fragilis LuxR gene, involved in quorum sensing, among colitis patients in Mosul, Iraq","authors":"A. Sulaiman, Assala Saad","doi":"10.31018/jans.v16i1.4716","DOIUrl":"https://doi.org/10.31018/jans.v16i1.4716","url":null,"abstract":"Bacteroides fragilis is the most anaerobic bacteria that infect humans, particularly in the abdominal cavity. Its pathogenesis is linked to numerous virulence factors. Understanding these factors and exploring alternative options for the use of antibiotics in the treatment of this bacterium, molecular techniques offer several advantages over traditional culture techniques because they are easier and more specific. The present study aimed to use specific primers for the 16sRNA and LuxR genes to identify B. fragilis. Genetic identification of the B. fragilis isolates was performed using the 16SrRNA gene, and the obtained sequences were submitted to National Centre for Biotechnology Information (NCBI) with accession numbers (OQ448827, OQ448828). Each strain was assigned a unique strain name, AS. AWB94 and AS. AWB79. From the total of all samples, it was found that the growth of various types of bacteria constituted ( 76%), and the samples that did not have growth formed (24%). It was noted that Bacteroidetes constituted only two isolates (2.7%), and these two isolates possessed the gene for quorum sensing (luxR gene), while the results confirmed that they do not possess the sialidase (nanH) enzyme gene. Both isolates possessed the quorum sensing gene (LuxR) out of one hundred samples. This suggests that the isolates have a quorum-sensing mechanism responsible for cell-to-cell communication, multidrug resistance, and biofilm formation.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of gibberellic acid (GA3) and temperature on seed germination of Capparis spinosa L. 赤霉素（GA3）和温度对 Capparis spinosa L.种子萌发的影响

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5287

Basma Chiboub, Abdesselam Maatougui, Kaoutar Aboukhalid, Said Otouya, Fatima Zarqi, Abderrahmane Nazih, Mourad Baghour

{"title":"Effect of gibberellic acid (GA3) and temperature on seed germination of Capparis spinosa L.","authors":"Basma Chiboub, Abdesselam Maatougui, Kaoutar Aboukhalid, Said Otouya, Fatima Zarqi, Abderrahmane Nazih, Mourad Baghour","doi":"10.31018/jans.v16i1.5287","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5287","url":null,"abstract":"Seed germination of Capparis spinosa L. is highly important for ecology, medicine, and economics. The present study aimed to determine the effects of six pretreatments and two temperature regimes, T1: 9/35.7°C (Laboratory) and T2: 1/43°C (Greenhouse), on Capparis spinosa L. seeds' germination rate and latency time. Different pretreatments were tested, including scarification (P1) and seed imbibition in water (P2 and P3) and gibberellic acid (GA3) (P4, P5 and P6).The results showed that the highest germination rate (68.33%) was observed in the laboratory for the control seeds (T1, P0), followed by (58,33%) for seeds soaked in water for 48 hours (T1 P3),(56,67%) for seeds soaked in 200 ppm ofGA3 (T1, P4),(53,33%)for seeds soaked in 400 ppm and 600 ppm of GA3 (T1, P5andT1, P6), (48,33%)for seeds soaked in water for 24 hours (T1 P2), and (51,43%) for the control seeds in greenhouse (T2, P0), whereas the lowest germination rate (12.86%) was recorded in the greenhouse temperature, which was detrimental to seed germination, for seeds soaked in 600 ppm of GA3 (T2, P6). Germination latency times were shorter after soaking the seeds in water for 24 hours. Finally, seed germination of C. spinosa is subjected to several factors that may influence the total percentage of germination and latency time.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of antidiabetic potential of Hibiscus rosa sinensis on streptozotocin-induced diabetes on Wistar albino rats 评估中华芙蓉对链脲佐菌素诱导的 Wistar 白化大鼠糖尿病的抗糖尿病潜力

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5334

K. Chauhan, S. Rani, Article Info

{"title":"Evaluation of antidiabetic potential of Hibiscus rosa sinensis on streptozotocin-induced diabetes on Wistar albino rats","authors":"K. Chauhan, S. Rani, Article Info","doi":"10.31018/jans.v16i1.5334","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5334","url":null,"abstract":"The chronic metabolic disease known as diabetes mellitus causes hyperglycemia in the body. Antioxidant and antidiabetic qualities are well-known benefits of Hibiscus rosa sinensis (HRS). In this work, diabetic Wistar albino rats were used to assess the antidiabetic properties of HRS flower extract. A total of 18 animals were taken and divided into three groups (n = 6) – (Group 1): Normal control group, (Group 2): Diabetic control group, (Group 3): Diabetic group treated with a 125 mg/kg dose of HRS flower extract. Group 2 animals showed a progressive decrease in body weight, while Group 1 animals showed a considerable gain in body weight. After overcoming the weight loss, Group 3 animals also showed an increase in body weight that was similar to Group 1. Group 2 animals had blood glucose levels higher than 400 mg/dL, but Group 1 animals had blood glucose levels below 200 mg/dL throughout the experiment. Group 3 animals first had glucose levels higher than 350 mg/dL and then lower than 200 mg/dL, comparable to Group 1 animals. Upon histological examination, the pancreatic islets of Group 2 animals showed vacuolation, necrosis, and degeneration. The animals in Group 3 displayed regenerated islets of Langerhans and enhanced pancreatic anatomy. The animals in Group 3 also returned to normal in terms of body weight and blood glucose levels, similar to those in Group 1. These findings show that Hibiscus rosa sinensis has potential as an alternative diabetic treatment; further research is needed to fully understand its modes of action and long-term effects.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficiency of Bacillus mucilaginosus isolated from the soil in dissolving potassium in its microenvironment 从土壤中分离出的粘液芽孢杆菌在其微环境中溶解钾的效率

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.4728

Noor S. Naji, Y. Jassim, Lilian Qasim Alwan Al-Budairi, Zainab Mohammed Abass

{"title":"Efficiency of Bacillus mucilaginosus isolated from the soil in dissolving potassium in its microenvironment","authors":"Noor S. Naji, Y. Jassim, Lilian Qasim Alwan Al-Budairi, Zainab Mohammed Abass","doi":"10.31018/jans.v16i1.4728","DOIUrl":"https://doi.org/10.31018/jans.v16i1.4728","url":null,"abstract":"Soil bacteria have an effective role in dissolving soil potassium. Bacillus mucilaginosus plays an effective role in dissolving potassium in the soil so that the plant may absorb it easily. The present study aimed to test the efficiency of bacteria in dissolving potassium present in the soil surrounding the roots of crops. B. mucilaginosus was isolated and diagnosed from the rhizosphere soil of Celery, Wheat, Basil and Alfalfa plants. The diagnosis included studying the isolates' culture, microscopic and biochemical characteristics. The laboratory study also included testing the efficiency of these bacterial isolates in dissolving potassium compounds in Modified Aleksandrov agar medium and estimating the dissolution coefficient. The results of isolation and identification of bacteria isolated from 19 out of 50 soil samples planted with different crops (Celery, Wheat, Basil and Alfalfa) showed that 8 isolates could dissolve potassium. The results of the microscopic examination of these eight isolates showed that they were sticky in shape, positive for Gram-staining, forming spores and the capsule, while the movement examination showed that they were positive for these tests (movement test). The biochemical tests and cultural characteristics showed that the eight isolates bear the characteristics of B. mucilaginosus. The results showed that the dissolution coefficient of potassium for the different isolates ranged between 2.28 and 1.14, while the type of sugar added to the culture medium increased the efficiency of bacterial isolates for potassium solubility. The study demonstrated the bacteria's efficiency in the rhizosphere region in dissolving potassium, which helps the plant use it easily.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a parametric-based Analytical Hierarchy Process (AHP) utilizing Geographic Information Systems (GIS) for wheat land suitability evaluation 利用地理信息系统（GIS）开发基于参数的分析层次过程（AHP），用于小麦土地适宜性评估

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5405

R. S. Makar, Sahar A. Shahin, Mohamed Abd El-Hady

{"title":"Development of a parametric-based Analytical Hierarchy Process (AHP) utilizing Geographic Information Systems (GIS) for wheat land suitability evaluation","authors":"R. S. Makar, Sahar A. Shahin, Mohamed Abd El-Hady","doi":"10.31018/jans.v16i1.5405","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5405","url":null,"abstract":"Wheat is considered one of the most essential crops for Egypt. Nevertheless, it is also one of its largest imports. Therefore, it is important to develop an accurate wheat suitability model to define the most suitable areas for its production. This study aimed to develop a parametric-based Analytical Hierarchy Process (AHP) using Geographic Information Systems (GIS) for land suitability evaluation of wheat in a selected area in El-Beheira governorate, Egypt. The climatic and land parameters influencing wheat production in the studied area were selected and rated according to the parametric method. These parameters included slope, texture, calcium carbonate, sum of basic cations, pH, organic matter, salinity, exchangeable sodium percentage and mean temp. of the growing cycle. The rated parameters were processed according to the AHP. The results were compared with Storie and the Square root methods and field observations. When validated using field observations, the developed method had a higher accuracy suitability evaluation for wheat cultivation in the studied area than the other two methods. According to the developed method, almost all of the studied areas could be classified as very suitable (S1) for wheat cultivation. On the other hand, the wheat suitability evaluation according to the other two methods indicated that most of the studied area could be classified as moderately suitable (S2) and marginally suitable (S3), which contradicted the field survey.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of vegetation cover change in the Southern region of Bangladesh using the Normalized Difference Vegetation Index (NDVI) and Climate Smart Agriculture (CSA) practices 利用归一化植被指数（NDVI）和气候智能型农业（CSA）做法检测孟加拉国南部地区的植被变化

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5398

Nazmun Naher, Jayan Saosan Zannat, Jahamina Jarin Sharna

{"title":"Detection of vegetation cover change in the Southern region of Bangladesh using the Normalized Difference Vegetation Index (NDVI) and Climate Smart Agriculture (CSA) practices","authors":"Nazmun Naher, Jayan Saosan Zannat, Jahamina Jarin Sharna","doi":"10.31018/jans.v16i1.5398","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5398","url":null,"abstract":"Bangladesh is extremely vulnerable to climate change, and vegetation indices serve as sensitive indicators. Due to the impacts of climate change, the cropping intensity of Southern region of Bangladesh is very low. So, this study aimed to analyze the changes in vegetation cover over time using the Normalized Difference Vegetation Index (NDVI) and identify the use of Climate Smart Agriculture (CSA) technologies and the benefits of using such technologies. A questionnaire survey was carried out by purposive random sampling method to detect 120 farmers’ socioeconomic status, hazards faced by climate change, adopted climate smart agricultural practices and its benefits for assessing Adaptive Strategy Index (ASI) in Amtali upazila of Barguna district and Kalapara upazila of Patuakhali district. NDVI analysis of multi-spectral remote sensing data from 2012 and 2022 indicated the extent of sparse vegetation of Kalapara has increased. Western part of Amtali upazila, fallow areas have become lessened in 2022 (354.55 km2) compared to 2012 (368.78 km2) due to adopting different CSA practices. Saline-tolerant crop varieties, sunflowers, and watermelon cultivation were the highest ranked among the CSA practices, with 301, 300, and 296 ASI, respectively. Calculated weighted average of CSA practices indicated the reduction of production cost, increased family income (49.19%) and cropping intensity (51.67%), which impacts developed social bonding.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigating of waterlogging associated problems by the management practices in the rice ecosystem of the Deltaic zone of Tamil Nadu 通过泰米尔纳德邦三角洲地区水稻生态系统的管理措施缓解与水涝相关的问题

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5273

K. Bama, S. Elamathi, T. Sivasankari, A. Anuratha, Article Info

{"title":"Mitigating of waterlogging associated problems by the management practices in the rice ecosystem of the Deltaic zone of Tamil Nadu","authors":"K. Bama, S. Elamathi, T. Sivasankari, A. Anuratha, Article Info","doi":"10.31018/jans.v16i1.5273","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5273","url":null,"abstract":"Cauvery Delta is the major rice-growing tract of Tamil Nadu. Continuous waterlogging is inevitable in the delta region due to unexpected heavy rain, leading to stunted crop growth and poor soil conditions. The present study aimed to alleviate the waterlogging-associated problems of stunted growth, crop nutrition deficiency, heavy algal growth and poor soil aeration issues in the heavy clayey soils of deltaic region of Tamil Nadu. Field experiments were laid out with the treatments viz., CuSO4,, (5kg/ha) (T1), Gypsum (500 kg/ha) (T2), Conoweeding+Alternate Wetting and Drying Irrigation(AWDI) (T3), microbial consortia (K and Zn solubilising Bacteria 500 ml/ha) (T4) control (T5) and combination of all (T6) except CuSO4 along with control . The results indicated that the T6 recorded higher plant height (110.2 cm), productive tillers/m2 of 332, filled grains of 118.3 and less chaffy grains of 20.3 nos, high nutrient status of 265 kg/ha of available N,35.4 kg/ha of available P,342 kg/ha of available K, 21.1meq/100 g of Ca,8.2 meq/100 g of Mg and root length and volume. The algal population in terms of dry biomass was reduced to 3.1 (g/m2) from the control group of 11.2 (g/m2) at 15 days after imposing treatment. The per cent increase of 26.0 % grain yield was also recorded in the combination treatment over control. From the present research, combined application of gypsum @500 kg/ha +Cono weeding twice +AWDI and microbial consortia of Zn and K solubilising bacteria @500ml/ha could be recommended for better soil environment and rice production in the delta region.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodiversity of intertidal mollusks in Surigao City, Philippines 菲律宾苏里高市潮间带软体动物的生物多样性

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5322

Mark Anthony T. Bangao, E. A. Borja, Romel C. Mutya

{"title":"Biodiversity of intertidal mollusks in Surigao City, Philippines","authors":"Mark Anthony T. Bangao, E. A. Borja, Romel C. Mutya","doi":"10.31018/jans.v16i1.5322","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5322","url":null,"abstract":"Intertidal zones are crucial ecological interfaces, but there is a lack of comprehensive studies on mollusk communities in Surigao City, Philippines. This research aimed to assess intertidal mollusk profiles, including species density, diversity, evenness, and richness, addressing a knowledge gap in regional mollusk biodiversity. Using a belt transect quadrat method in three sampling areas during 2021, the study employed a descriptive correlation design to investigate relationships between mollusk profiles and seawater physico-chemical parameters (conductivity, dissolved oxygen, pH, salinity, and temperature). In Surigao City's Day-asan, Hikdop Island, and Sibale, 44 gastropod species (19 families) and 11 bivalve species (9 families) were identified. Conidae and Strombidae families dominated. Average seawater parameters were conductivity 51.33, dissolved oxygen 7.45, pH 7.84, salinity 32, and temperature 28.52. Day-asan exhibited the highest species count (49), followed by Hikdop Island (42) and Sibale Island (37). Hikdop Island had the highest species density and evenness, Day-asan had the highest species diversity index, and Sibale Island had the highest species richness. Mollusk profiles showed no significant differences between sampling areas, and a strong negative correlation between pH and species evenness was observed. Surigao City displayed diverse mollusk biodiversity, but a declining trend in diversity indices was noted. This study fills a critical knowledge gap in understanding mollusk community dynamics, providing essential baseline data for future conservation and management strategies in the region, while also shedding light on the impact of environmental factors on intertidal mollusk populations in Surigao City.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of micronuclei in blood and histopathological alterations in gill, kidney and liver of Channa punctatus (Bloch, 1793) exposed to copper sulphate 暴露于硫酸铜的鳢鱼 (Bloch, 1793) 血液中微核的诱导以及鳃、肾和肝脏的组织病理学改变

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5172

Namita Kumari, Vivek Kumar, S. P. Trivedi, C. Singh, Article Info

{"title":"Induction of micronuclei in blood and histopathological alterations in gill, kidney and liver of Channa punctatus (Bloch, 1793) exposed to copper sulphate","authors":"Namita Kumari, Vivek Kumar, S. P. Trivedi, C. Singh, Article Info","doi":"10.31018/jans.v16i1.5172","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5172","url":null,"abstract":"Copper is one of the most toxic metals to fish and causes cytotoxic, mutagenic and carcinogenic effects. The accumulation of copper in an aquatic environment directly impacts man and the aquatic ecosystem. The present study aimed to determine the effect of copper sulphate (CuSO4) accumulation on the induction of micronuclei in blood and histological changes in kidney and liver of the fish Channa punctatus. The test chemical LC50 was determined after 96 hours during the first experiment. Later in the second experiment, the test animal was exposed to three sub-lethal concentrations of CuSO4 [0.1 mg/l (96 h-LC50/40), 0.2 mg/l (96 h-LC50/20) and 0.4 mg/l (96 h-LC50/10)]. Physico chemical parameters of the test medium, such as pH, temperature, hardness, alkalinity, and dissolved oxygen, were observed throughout the experiment, and all values were within the ranges necessary for the fish's survival. At intervals of 7, 14, 21, and 28 days, the control (without any test chemical) and copper sulphate exposed Groups' blood, liver, kidney, and gill tissues were taken to evaluate changes in genotoxic and histological parameters. Micronuclei (MN) induction and nuclear abnormalities (NAs) were observed at regular intervals. After 28 days, the MN frequency in Groups 1, 2, 3, and 4 was 0.78±0.006, 8.40±0.052, 10.37±0.098 and 10.90±0.024 respectively. A significant (P< 0.05) rise in MN frequencies and NAs indicated fish erythrocytes' DNA damage. Histological analysis of the liver, kidney, and gills revealed serious tissue injury such as necrosis, vacuolization, and degeneration after 28 days in the exposed Groups. The present study observed changes due to genotoxicity and histology, giving the most comprehensive understanding of CuSO4 stress on the fish C. punctatus and its risks to human health.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of the Mentha spicata leaves in reducing testosterone levels and aggressive behaviour in female Wistar albino rats treated with testosterone propionate 薄荷叶对降低丙酸睾酮治疗的雌性 Wistar 白化大鼠体内睾酮水平和攻击行为的效果

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5279

Zahraa A. Taha, Halla Abdul-Hadi Chabuk, Alaa Jawad Hassan

{"title":"Effectiveness of the Mentha spicata leaves in reducing testosterone levels and aggressive behaviour in female Wistar albino rats treated with testosterone propionate","authors":"Zahraa A. Taha, Halla Abdul-Hadi Chabuk, Alaa Jawad Hassan","doi":"10.31018/jans.v16i1.5279","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5279","url":null,"abstract":"High testosterone hormone levels play an important role in exhibiting aggressive behaviour and several disorders in female rodents and women. The present study aimed to determine the protective role of the alcoholic extract of Mentha spicata leaves (MSL) in the reduction of the level of testosterone and aggressive behaviour of female rats that suffer from high levels of testosterone. A total of 30 female Wistar albino rats were divided into 6 Groups. Group 1: Control rats received sesame oil(0.5 ml). Group 2: Rats injected with testosterone propionate (TP) alone. Group 3: Rats received MSL alone (100 mg/kg) orally. Groups 4, 5, and 6: Rats received TP + MSL, 6mg/rat of TP followed by 200, 400, and 600 mg/rat, respectively) for 60 days. The testosterone, dopamine, and aggressive behaviour were measured using specialized ELISA test kits. The results showed that testosterone and dopamine levels in the serum had a significant decrease (P<0.05) (4.82 (ng/ml) in testosterone and 272.83 (pg/ml) in dopamine) in animals treated with MSL only compared to Group 2, which found a significant rise (p<0.05) (16.52 (ng/ml) in testosterone and 607.59 (pg/ml) in dopamine) in the levels of testosterone and dopamine. The results exhibited a significant rise (P<0.05) in the aggressive behaviour in Group 2 of rats compared to the control and other Groups. In comparison, aggressive behaviour was significantly decreased (P<0.05) (7.40 (ng/ml) in testosterone and 263.49 (ng/ml) in dopamine) in Groups 2, 4, 5, and 6. Thus, the study revealed the protective role of the alcoholic extract of MSL in reducing levels of testosterone and aggressive behaviour in female rats suffering from higher levels of testosterone.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0