Journal of Applied and Natural Science最新文献_第3页

Effect of chemical mutagen (Ethyl methane sulfonate) on Money plant (Epipremnum aureum) 化学诱变剂（甲烷磺酸乙酯）对金钱草的影响

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.4736

Shivani More, H. Sahare

{"title":"Effect of chemical mutagen (Ethyl methane sulfonate) on Money plant (Epipremnum aureum)","authors":"Shivani More, H. Sahare","doi":"10.31018/jans.v16i1.4736","DOIUrl":"https://doi.org/10.31018/jans.v16i1.4736","url":null,"abstract":"Epipremnum aureum, commonly known as pothos, is a hardy indoor foliage ornamental crop. Mutation breeding in Golden pothos is needed to meet the demand for more valuable and highly sought-after cultivars. The present study aimed to exploit variability on various parameters viz. new leaf emergence, survival percentage, plant height, length and width of leaves. The experiment was set up using a Complete Randomized Design (CRD) with 8 treatments and 3 replications. Rooted cuttings of Golden pothos were treated with varying concentrations of Ethyl Methane Sulfonate (EMS) for 4 hours, and a control treatment was included. The results showed that all the treated cuttings with EMS delays the new leaf emergence period compared to the control. Compared to the control, the minimum number of new leaf emergences was found in EMS-treated plants. The maximum survival percentage (100%) was recorded in 0.1%, 0.5% and control treatment. The lowest plant height (15.3 cm ) was observed in treatment T4 (1.5 % EMS) treated plants. However, maximum plant height was observed in the control treatment at 150 days of interval. In comparison to the control, EMS decreased the length and width of leaves at higher dosages. The minimum leaf length and width, i.e. 2.40 cm and 1.20 cm, were recorded in plants treated with EMS at 2%, while maximum length and width were observed in control and low EMS-treated plants. The study showed that ethyl methane sulfonate affected plant development characteristics and showed some dwarfness of money plants. This appears to be the first report of EMS treatment on pothos.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 39","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality and functional attributes of muffins with incorporation of fruit, vegetable, and grain substitutes: A review 加入水果、蔬菜和谷物替代品的松饼的质量和功能特性：综述

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5330

Divyanshi Shukla, B. N. Tewari, S. P. Trivedi, Shraddha Dwivedi, Vivek Kumar, Vidyanand Tiwari

{"title":"Quality and functional attributes of muffins with incorporation of fruit, vegetable, and grain substitutes: A review","authors":"Divyanshi Shukla, B. N. Tewari, S. P. Trivedi, Shraddha Dwivedi, Vivek Kumar, Vidyanand Tiwari","doi":"10.31018/jans.v16i1.5330","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5330","url":null,"abstract":"Raw ingredients directly affect the quality of processed foods. Along with improvements in processing technology, processed food production has grown significantly. The percentage of bakery products in total food consumption is very high, and soft bakery foods despite having a short shelf life, are well-liked because of their deliciousness. Special attention is paid to muffins with various combinations of nutritive ingredients and organoleptic qualities. Recent research studies have extensively covered the use of various fruits, vegetables, and grain and their by products as raw materials and value-added incorporation in the muffin to produce a nutrient-rich and highly valuable muffin. The present study provides a detailed observation of three major categories of incorporated ingredients, including fruits, vegetables, and their processing by products, as well as some other grain-based ingredients used as raw materials that add value. The study compiles a brief for their use in baked goods while reviewing the literature on ingredients. The findings of the literature analysis show an increase in the nutritional content of bioactive substances like antioxidants in the muffins. It has been revealed that using ingredients derived from fruits and vegetables is safer and more effective in terms of nutrition. Muffins may have a higher quality and better nutritional profile when certain combinations of fruit- and vegetable-based ingredients are added to the main ingredient. It will be helpful to researchers, food manufacturers, and small and medium-scale bakery unit operators about various aspects of the raw materials and properties connected with new muffin formulations and the standardization of stabilized products.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contribution to the optimization of citrus (Citrus clementina Hort. Ex Tanaka) fruit fertilization using mobile lysimetry in orchards of the Souss-Massa region, Morocco 在摩洛哥 Souss-Massa 地区的果园中使用移动溶液测量法优化柑橘（Citrus clementina Hort.

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5307

Abdallah Zayani, Salah-Eddine Laasli, Rachid Lahlali, Fatima El Bouchtaoui, N. E. Alem

{"title":"Contribution to the optimization of citrus (Citrus clementina Hort. Ex Tanaka) fruit fertilization using mobile lysimetry in orchards of the Souss-Massa region, Morocco","authors":"Abdallah Zayani, Salah-Eddine Laasli, Rachid Lahlali, Fatima El Bouchtaoui, N. E. Alem","doi":"10.31018/jans.v16i1.5307","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5307","url":null,"abstract":"The growth and development of citrus trees and the quality of their fruits are significantly influenced by the essential role of mineral plant nutrition. This study aimed to improve the productivity of the citrus (Citrus clementina Hort. Ex Tanaka) orchards by optimizing mineral nutrition through well-monitored fertilization and fertigation aspects using lysimetry. The first phase consisted of studying the behavior of the nutrients in the soil solution at the high root concentration level, analysis of the nutritional status of five varieties of clementines (Sidi Aissa, Cadoux, Orogrande, Nules, and Nour), and the variation of leaf composition in major elements (Nitrogen, phosphorus, potassium, magnesium, and calcium) for the five varieties along phenological stages, through the exploitation of the results of analyses carried out for the management of mineral nutrition in 47 plots of citrus fruit in the Souss-Massa region with the use of lysimetry. The results obtained in this first part revealed an important variation of the nutrients in the soil solution (55.24%) in terms of water inputs, fertilizers, and edaphic conditions, as well as a large variation of foliar compositions (62.98%). The second phase, consisting of regular monitoring of the mineral nutrition dynamics targeting the “Nules” variety grafted on “Citrus macrophylla” affirmed the importance of the citrus fertilization approach for determining the availability, distribution, nutrient interactions in soil solution and plant response by regular leaf diagnostics. Thus, mobile lysimetry offered a powerful tool for achieving both productivity and sustainability in citrus fertilization programs.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 25","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yield assessment of rice-black gram, rice-maize and rice-groundnut sequential cropping system influenced by rice establishment methods and nutrient management practices in the dry tract of the Southern zone of Tamil Nadu, India 印度泰米尔纳德邦南部干旱地区受水稻种植方法和养分管理措施影响的水稻-黑粒李、水稻-玉米和水稻-花生连作系统的产量评估

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5223

S. Swathi, C. Ravikumar, M. Thiruppathi, P. Senthilvalavan, Article Info

{"title":"Yield assessment of rice-black gram, rice-maize and rice-groundnut sequential cropping system influenced by rice establishment methods and nutrient management practices in the dry tract of the Southern zone of Tamil Nadu, India","authors":"S. Swathi, C. Ravikumar, M. Thiruppathi, P. Senthilvalavan, Article Info","doi":"10.31018/jans.v16i1.5223","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5223","url":null,"abstract":"Integrated nutrient management (INM) practices and suitable cropping systems are an effective and promising way for enhancing crop productivity. However, the impact of proper INM practices and cultivation methods in rice cropping systems in dry tracts remains unclear. The field study evaluated the suitable cropping system under different rice establishment methods with INM practices in a sequential cropping system( rice-black gram, rice-maize, and rice-groundnut). Treatments were designed as two rice establishment methods [Direct Seeded Rice (DSR) and Transplanted Rice (TPR)] and five INM practices [RDF-Recommended Dose of Fertilizer (120:40:40 kg NPK ha-1) alone, RDF + Poultry manure compost @ 5 t ha-1, RDF+ Coir pith compost @ 5 t ha-1, RDF+ Green manure @ 6.25 t ha-1 and RDF-Green leaf manure @ 6.25 t ha-1]. TPR with the INM practice of RDF + poultry manure compost @ 5 t ha-1 recorded maximum productive tillers m-2 (351), number of filled grains panicle-1 (130.2), grain yield (6709 kg ha-1) and straw yield (9015 kg ha-1) of rice. Rice fallow crops (black gram, maize and groundnut) recorded 10 – 24,10-18 and 10 - 21 % higher values of yield components and yield, respectively, in DSR with INM treatment of RDF + Green manure @ 6.25 t ha-1 . Thus, RDF plus green manure or poultry manure application is an apt INM practice for a rice-based sequential cropping system under DSR, and the rice-black gram is an economical and sustainable cropping system for dry tracts of the southern zone of Tamil Nadu. \u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-cancer activity of crude Slrp protein conjugated mesoporous silica nanoparticles in HeLa Cell Lines: An in vitro approach 粗制 Slrp 蛋白共轭介孔二氧化硅纳米粒子在 HeLa 细胞系中的抗癌活性：体外方法

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5285

Shivaprasad Panjala, Satyanarayana Swamy Vyshnava, Swathi Banapuram, Vihari Vasikarla, Paramasivam Kameshpandian, Muralidhara Rao Dowlathabad, R. Anupalli

{"title":"Anti-cancer activity of crude Slrp protein conjugated mesoporous silica nanoparticles in HeLa Cell Lines: An in vitro approach","authors":"Shivaprasad Panjala, Satyanarayana Swamy Vyshnava, Swathi Banapuram, Vihari Vasikarla, Paramasivam Kameshpandian, Muralidhara Rao Dowlathabad, R. Anupalli","doi":"10.31018/jans.v16i1.5285","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5285","url":null,"abstract":"Microbial based therapeutics for cancer have gained a significant interest in recent decades. The present study relies on the synthesis, analysis, and conjugation of Salmonella Leucine-rich Proteins (SlrP) with mesoporous silica nanoparticles (MSNN) to evaluate their potential anticancer activity. The SlrP proteins were effectively produced and isolated from Salmonella enterica using Tryptic Soy Broth (TSB), and the subsequent SDS-PAGE analysis verified the presence of a band at around 72 KDa. The MSN synthesis yielded particles with an average diameter of 68.05±0.87 nm and a pore diameter of 7.1 nm. In addition, we synthesized MSNMPA and then conjugated them with SlrP. Characterization studies confirmed the effective conjugation. The cytotoxicity evaluation conducted on HeLa cells revealed no substantial modification in cell viability upon treatment with MSN alone. Nevertheless, when MSNMPA/SlrP was done, it demonstrated significant cytotoxic properties, as evidenced by an IC50 value of 10 µg/mL. The results indicate that SlrP-conjugated MSN (MSNMPA/SlrP) could be utilized as promising nanocarriers for delivering anticancer proteins.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing sustainability and flavor of waste coconut (Cocos nucifera) kernel and sweet potato (Ipomoea batatas) peels Creamy roll: Extension program guide 提高废弃椰子（Cocos nucifera）仁和甘薯（Ipomoea batatas）皮的可持续性和风味奶油卷：推广计划指南

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5396

Sutero S. Macabudbud, Jr.

{"title":"Enhancing sustainability and flavor of waste coconut (Cocos nucifera) kernel and sweet potato (Ipomoea batatas) peels Creamy roll: Extension program guide","authors":"Sutero S. Macabudbud, Jr.","doi":"10.31018/jans.v16i1.5396","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5396","url":null,"abstract":"Waste Coconut Kernels (WCK) and Sweet Potato (SP) peels are considered household wastes for animal feeding. The present study aimed to develop waste coconut kernel and sweet potato peels creamy roll as innovative guides for the Extension program guide. This experimental study used three formulations varying amounts of WCK with 100g, 150g and 200g; SP peels with 150g, 200g, and 250g mixed with milk solution, sugar and unsalted spring roll wrappers. The study focused on investigating the sensory attributes of the creamy roll development across different formulations, assessing its general acceptability in terms of color, flavor, odor and texture, identifying the most preferred formulation (F1, F2, and F3) based on the panel of tasters, and examining if there were significant differences among the product attributes. Based on Data analysis involved calculating weighted means and conducting One-way analysis of variance (ANOVA), the results revealed that F3, consisting of 250g of SP, 200g of WCK, 250g of milk solution, and 180g of sugar, was the most preferred in terms of flavor, odor and texture, with overall means of 3.67, 4.08, and 3.85, respectively. However, F2 was preferred only in color. A significant difference was observed in flavor indicated by a p-value of .042, lower than the predetermined alpha level of .05. Post-hoc analysis indicated differing perceptions between the 35 experienced consumers and 15 food experts. The most preferred formulation had a sensory acceptability rating of “acceptable” with a mean of 3.92. Based on the findings, the 250g SP peels and 200g WCK were adopted as an Extension program guide.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of anticancer effect of Cladophora glomerata algae extract 藻类提取物的抗癌效果评估

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.4978

Dhuha Muhammed Sadik, Ibrahim Hadi Mohammed

{"title":"Evaluation of anticancer effect of Cladophora glomerata algae extract","authors":"Dhuha Muhammed Sadik, Ibrahim Hadi Mohammed","doi":"10.31018/jans.v16i1.4978","DOIUrl":"https://doi.org/10.31018/jans.v16i1.4978","url":null,"abstract":"Cladophora glomerata has numerous biologically active properties and is considered one of the most essential medicinal algae. The purpose of this research was to investigate the anticancer efficacy of Cladophora glomerata algae extract against human hepatocellular carcinoma (HepG2), human cervical carcinoma (HeLa), and normal mouse embryonic fibroblast (MEF) cell lines. The needed algae was found in the Hassan Al-Hamoud River , Baquba, Iraq. The chemical detection of some chemical components of the ethanolic extract of C. glomerata revealed that the extract contains a group of active compounds. The study showed significant variation (p<0.05) among inhibition percentages of cancer cell line HepG2, Hella, and MEF cell lines that were treated with different concentrations (15.1, 31.2, 62.5, 125, 250, 500, and 1000) μg/ml of C. glomerata extract. The outcomes showed that increased inhibition percentages of the above cell line were associated with increased concentrations. The inhibition percentage of HepG2, Hella, and MEF cell lines were 1.6 ± 30.88, 1.7 ± 14.10, and 1.2 ± 2.31 at concentration 15.1 (mg/ml), and was 3.8 ± 84.90, 3.6 ± 88.29, and 3.9 ± 23.2, respectively at concentration 1000 (mg/ml). The study concluded that the C. glomerata extract with different concentrations significantly inhibited cancer cell lines (HepG2 and Hela) and ME because they have antiproliferative and antioxidant activity against cancers. The extract's inhibitory impact begins at low doses and increases with increasing concentration. The study would be beneficial to use macroalgae as new and sustainable sources of bioactive compounds against cancer.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-oxidant and cytotoxic activity of Spirulina platensis ethanolic extract against Caco-2 and HepG2 cancer cell lines 板蓝根螺旋藻乙醇提取物对 Caco-2 和 HepG2 癌细胞系的抗氧化和细胞毒性活性

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5280

Hala M.N. Al-saily, Walaa Salih Hassan, H. Chabuk

{"title":"Anti-oxidant and cytotoxic activity of Spirulina platensis ethanolic extract against Caco-2 and HepG2 cancer cell lines","authors":"Hala M.N. Al-saily, Walaa Salih Hassan, H. Chabuk","doi":"10.31018/jans.v16i1.5280","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5280","url":null,"abstract":"Spirulina platensis is blue-green algae received significant attention for its high nutritional value, it is a source of powerful antioxidants. The cytotoxicity of crude extract is not well recorded. The aim of current study to evaluate the cytotoxicity of S. platensis extracts on colon cancer (CaCo-2), hepatic cancer (HepG2) cell lines, normal fibroblast cells line (HdFn) and also antioxidant activity. The percent of 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity was determined for serial concentrations of extract ranging from 3.125 to 200 μg/ml. Cell lines were treated for 24 hours with different concentrations of extract ranging from 25 to 400 µg/ml. Cell viability testing using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay which determined how the extract affected caspase 9 activity. The results revealed that extract had moderate antioxidant activity, showing the DPPH scavenging activity reached 58% in a concentration of 200μg/ml, and IC50 was 95.84 μg/ml. The extract significantly decreased CaCo-2 cell viability with IC50 99.12 µg/ml, compared to HdFn viability with IC50 157.6 µg/ml. On CaCo-2 cells, the extract's cytotoxicity was more evident (P< 0.05) than HdFn cells. The extract had more significant (P<0.05) cytotoxicity on cancer cell lines and also significantly decreased the viability of HepG2 cells with IC50 167.4 µg/ml, than the viability of HdFn with IC50 214.9 μg/ml. The extract revealed significantly higher (P< 0.05) cytotoxicity against HepG2 cells than the normal HdFn cells. This study concluded that the extract exerted a dose-dependent anti-proliferation effect on CaCo-2 cells and HepG2 cells by comparing them with HdFn cells.","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of Azolla in organic farming on availability and uptake of N, P, K of rice paddy (Oryza Sativa, L.) 有机耕作中 Azolla 的使用对水稻（Oryza Sativa, L.）氮、磷、钾的供应和吸收的影响

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5336

S. Suntoro, Ganjar Herdiansyah, S. Minardi, Sri Hartati, Cindy Wulan Sari, Fidia Damayanti, Oktione Ismi Ardhasista

{"title":"Use of Azolla in organic farming on availability and uptake of N, P, K of rice paddy (Oryza Sativa, L.)","authors":"S. Suntoro, Ganjar Herdiansyah, S. Minardi, Sri Hartati, Cindy Wulan Sari, Fidia Damayanti, Oktione Ismi Ardhasista","doi":"10.31018/jans.v16i1.5336","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5336","url":null,"abstract":"Organic fertilization is very important to maintain sustainable land and environmental productivity. Organic fertilizer from Azolla has the advantage of increasing soil fertility by increasing nutrient availability. This study aimed to determine the effect of Azolla application on the availability and uptake of N, P, and K of Inpari 32 variety rice plants. This research used 9 treatments: (T0= control, T1= 90 kg/h NPK, T2= 45 kg/h NPK, T3= 10 t/h Manure, T4= 2 t/h Manure + 90 kg/h NPK, T5= 10 t/h Rice straw compost, T6= 2 t/h Rice straw compost + 90 kg/h NPK, T7= 10 t/h Azolla, T8= 2 t/h Azolla + 90 kg/h NPK) with as control and 3 replications, so there were 27 experimental plots. This research was located in the paddy field of Madiun Regency, East Java. The results showed that Azolla, manure, and straw compost at a dose of 10 t/ha increased soil C-organic, soil Cation Exchange Capacity (CEC), and soil pH, which was still in the neutral range. Azolla fertilizer increased the highest soil total N compared to cow manure and straw compost and increased the efficiency of NPK fertilizer use. Azolla (T7) increased soil available P (27.02 ppm) and exchangeable K (17.63 me/100g soil). Azolla had the highest available P effect. Azolla (T7) and manure (T4) were affected by increasing K-exchange. Azolla fertilizer, manure, and rice straw compost increased vegetative plant growth (plant height, number of tillers, shoot and root biomass). Among the three, Azolla fertilizer had the highest effect. Organic fertilization affected N, P, and K uptake, which was highest in Azola fertilizer (T7). For manure, varied NPK fertilizer (T4) just showed an increase in P uptake, and compost fertilizer varied NPK fertilizer (T6) showed a rise in K uptake. A positive relationship existed between the availability and uptake of nutrients N, P, and K. \u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Active compounds of Michelia champaca bark extract against Curvularia verruculosa fungi causing leaf spot disease in rice (Oryza sativa L.) Michelia champaca 树皮提取物中的活性化合物对引起水稻叶斑病的 Curvularia verruculosa 真菌的作用

Journal of Applied and Natural Science Pub Date : 2024-03-20 DOI: 10.31018/jans.v16i1.5406

Gusti Agung, Gede Bawa, Sri Rahayu, Santi, Wiwik Susanah, Olan Suryanadi, Gek Indyan, Article Info

{"title":"Active compounds of Michelia champaca bark extract against Curvularia verruculosa fungi causing leaf spot disease in rice (Oryza sativa L.)","authors":"Gusti Agung, Gede Bawa, Sri Rahayu, Santi, Wiwik Susanah, Olan Suryanadi, Gek Indyan, Article Info","doi":"10.31018/jans.v16i1.5406","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5406","url":null,"abstract":"Curvularia verruculosa fungal causes leaf spot disease in rice plants. The bark extract of Michelia champaca could inhibit the growth of the fungi. The present research aimed to know the active compound responsible for antifungal activity. Extraction was done using the Maceration method, antifungal activity was measured using the Diffusion well method, and identification of active compounds was carried out using Gas chromatography-mass spectroscopy (GC-MS). The methanol extract obtained had a yield of 18.7%. It showed strong activity against C. verruculosa with an inhibition zone until 30.01 mm. The fractionation results showed that n-hexane extract (HE) was the strongest inhibiting the growth of C. verruculosa (32.45 mm), followed by chloroform extract (CE) (29.20 mm), while n-butanol extract (BE) was not active. Separating active compounds from HE extract was made using Column chromatography (CC) method with silica gel as the stationary phase and the mixture of n-hexane-acetone (3:1) as the mobile phase. This separation resulted in 5 combined fractions; HE3 and HE5 extracts showed very strong activity against C. verruculosa, with a diameter of the inhibition zone of 26.73 and 33.46 mm, respectively; HE2 extract showed strong activity with a diameter of the inhibition zone of 15.21 mm, while HE1 and HE4 extracts did not show activity. Identification using GC-MS, especially the HE3 extract, revealed that the extract contained two compounds: tributyl acetyl citrate and terephthalic acid, dodecyl-2-ethylhexyl ester. The result indicated that the bark extract of M. champaca had the potential to be a botanical fungicide.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0