Journal of applied biochemistry最新文献_第7页

Rapid modulation of platelet aggregation in plasma by oxygenated sterols. 氧合甾醇对血浆血小板聚集的快速调节。

Journal of applied biochemistry Pub Date : 1984-06-01

H Shimada, T Imada, T Kikuchi, Y Inada, M Morisaki, N Ikekawa, Y Saito

引用次数: 0

The wobble rule. 抖动法则。

Journal of applied biochemistry Pub Date : 1984-06-01

T H Jukes

引用次数: 0

Acetylcholine. 乙酰胆碱。

Journal of applied biochemistry Pub Date : 1984-02-01

T H Jukes

引用次数: 0

Construction of a system for the regeneration of adenosine 5'-triphosphate, which supplies energy to bioreactor. 为生物反应器提供能量的5′-三磷酸腺苷再生系统的构建。

Journal of applied biochemistry Pub Date : 1984-02-01

H Kondo, I Tomioka, H Nakajima, K Imahori

{"title":"Construction of a system for the regeneration of adenosine 5'-triphosphate, which supplies energy to bioreactor.","authors":"H Kondo, I Tomioka, H Nakajima, K Imahori","doi":"","DOIUrl":"","url":null,"abstract":"An engineering model was successfully developed for an ATP regeneration system by using two enzymes, acetate kinase (AK) and adenylate kinase (AdK), both obtained from the thermophile Bacillus stearothermophilus. This model is composed of five units: a substrate unit consisting of substrate solutions--AMP, ATP, and acetyl phosphate (AcOP)--an enzymatic reactor unit consisting of AK and AdK immobilized to Sepharose 4B, an auto sampler unit, an analytical unit made up of high-performance liquid chromatography, and a control unit made up of a microcomputer. Operation of the four units could be systematically controlled by the microcomputer. Fundamental, operational conditions were examined using this engineering model. The conversion of AMP to ATP concentration and space velocity (SV). The minimum amount of ATP, which is required to obtain the 100% conversion of AMP to ATP, was determined to be about 4% of AMP concentration. The conversion of AMP to ATP was controlled effectively by changing the SV value. Based on the above experimental data, the continuous operation of an ATP regeneration system was tested at pH 7.5 and 30 degrees C under the conditions of 1.59 mM AMP, 0.084 mM ATP, and 5.0 mM AcOP. It was found that the conversion of AMP to ATP was more than 99% over a period of 6 days without changing SV.","PeriodicalId":14978,"journal":{"name":"Journal of applied biochemistry","volume":"6 1-2","pages":"29-38"},"PeriodicalIF":0.0,"publicationDate":"1984-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17548900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of gamma-ray irradiation on superoxide dismutase activity and lipid peroxide level in mouse salivary glands. γ射线照射对小鼠唾液腺超氧化物歧化酶活性和过氧化脂质水平的影响。

Journal of applied biochemistry Pub Date : 1984-02-01

S Akita, M Nagayama, T Kaneda, T Oka, N Ohishi, K Yagi

引用次数: 0

Alteration of the substrate specificity of Aspergillus oryzae beta-galactosidase by modification with polyethylene glycol. 聚乙二醇修饰米曲霉β -半乳糖苷酶底物特异性的改变。

Journal of applied biochemistry Pub Date : 1984-02-01

M Naoi, K Kiuchi, T Sato, M Morita, T Tosa, I Chibata, K Yagi

{"title":"Alteration of the substrate specificity of Aspergillus oryzae beta-galactosidase by modification with polyethylene glycol.","authors":"M Naoi, K Kiuchi, T Sato, M Morita, T Tosa, I Chibata, K Yagi","doi":"","DOIUrl":"","url":null,"abstract":"beta-Galactosidase (beta-D-galactoside galactohydrolase, EC 3.2.1.23) purified from Aspergillus oryzae was modified with 2,4,6-trichloro-s-triazine derivatives of polyethylene glycol (activated BPEG) having molecular weights of 600, 1500, 2000, and 4000. Polyethylene glycol derivatives were attached to 6 of the 12 amino groups exposed on the surface of the enzyme. Upon modification, the enzymatic activity for a water-soluble substrate, o-nitrophenyl beta-D-galactopyranoside, was reduced with increasing molecular weight of the activated BPEG. On the contrary, the enzymatic activity for another substrate, 4-methylumbelliferyl beta-D-galactopyranoside, was increased upon modification. The Michaelis constants of native and modified enzymes for these two substrates were virtually the same. The effect of the modification was more marked in the enzymatic hydrolysis of the beta-galactosidic bond of amphipathic substrates. A fluorescent analog of naturally occurring galactocerebroside, 1-O-galactosyl-2-N-(1-dimethylaminonaphthalene-5-sulfonyl)-sphingosine, was hydrolyzed more rapidly by the modified enzyme than by the native one. The enzyme modified with activated BPEG of 1500 Da had the highest activity for this substrate. The beta-galactosidic bond of the terminal galactose of GM1-ganglioside (II3NeuAcGgOse4Cer, galactosyl-N-acetylgalactosaminyl-(N-acetylneuraminosyl)-galactosyl -glucosylceramide) was cleaved by the modified but not by the native enzyme.","PeriodicalId":14978,"journal":{"name":"Journal of applied biochemistry","volume":"6 1-2","pages":"91-102"},"PeriodicalIF":0.0,"publicationDate":"1984-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17495611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enantiospecificity of immobilized horse liver alcohol dehydrogenase. 固定化马肝醇脱氢酶的对映体特异性。

Journal of applied biochemistry Pub Date : 1984-02-01

H Görisch, W Boland, L Jaenicke

引用次数: 0

Conversion of cassava starch to biomass, carbohydrates, and acids by Aspergillus niger. 黑曲霉将木薯淀粉转化为生物质、碳水化合物和酸。

Journal of applied biochemistry Pub Date : 1984-02-01

K H Tan, L B Ferguson, C Carlton

{"title":"Conversion of cassava starch to biomass, carbohydrates, and acids by Aspergillus niger.","authors":"K H Tan, L B Ferguson, C Carlton","doi":"","DOIUrl":"","url":null,"abstract":"The filamentous fungus, Aspergillus niger, efficiently converted cassava polysaccharides to mycelial mass, simple sugars, and acids during the course of its growth. A typical 70-ml culture broth containing 2% cassava polysaccharides yielded 0.38 g dry mycelial mass, 1.14 mmol reducing sugars, and 1.17 meq acids at the end of 42 h. About 70% of the initial total carbohydrate in the medium was degraded during the same period. The sugars and acids in the culture broths were analyzed by HPLC on a single Aminex HPX-87 column at 55 degrees C, using 0.013 N H2SO4 as the eluting solvent. Cassava polysaccharides were degraded to oligosaccharides, maltotriose, maltose, and glucose beyond the 20-h growth periods, with maltotriose emerging as the major simple sugar. The appearance of citric, malic, gluconic, succinic, and fumaric acids accounted mostly for the decreasing pH in the growth media. Formation of carbohydrate species in the culture broths was closely related to the biosynthesis and secretion of several carbohydrases by A. niger. The extracellular carbohydrases were separated and identified by chromatofocusing and polyacrylamide gel electrophoresis to be amyloglucosidase (EC 3.1.2.3), alpha-amylase (EC 3.2.1.1), and alpha-glucosidase (EC 3.2.1.20), respectively.","PeriodicalId":14978,"journal":{"name":"Journal of applied biochemistry","volume":"6 1-2","pages":"80-90"},"PeriodicalIF":0.0,"publicationDate":"1984-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17547996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of monoclonal antibodies to human chorionic gonadotropin by a facile cloning procedure. 人绒毛膜促性腺激素单克隆抗体的简易克隆制备。

Journal of applied biochemistry Pub Date : 1984-02-01

C Z Teh, E Wong, C Y Lee

{"title":"Generation of monoclonal antibodies to human chorionic gonadotropin by a facile cloning procedure.","authors":"C Z Teh, E Wong, C Y Lee","doi":"","DOIUrl":"","url":null,"abstract":"A facile hybridoma procedure has been used to generate monoclonal antibodies to alpha- and beta-subunits of human chorionic gonadotropin (HCG). The procedure is based on the method of Davis et al. (1982, J. Immunol. Methods 50, 161) and involves the use of a semisolid medium containing methylcellulose for the initial cloning of hybrid cells following immunization and cell fusion. Seven to ten days after cell fusion, viable hybrid clones were removed for subculture in a liquid medium containing RPMI 1640 and 15% fetal calf serum. Initial screening of hybrid cell lines that secrete antibodies to HCG was performed on microplate enzyme-linked immunoassay (ELISA) using HCG-coated microtiter plates. The specificity of these antibodies to either alpha- or beta-subunits was determined by the sodium dodecyl sulfate-gel/protein blot radioimmunobinding method which separates alpha- and beta-subunits of HCG on nitrocellulose strips for radioimmunobinding assay. As a result of this study, it has been possible to generate about 272 hybrid cell lines that secrete antibodies reacting with either the alpha- or beta-subunit of HCG in about 5 weeks. The association constants and cross-reactivities to luteinizing hormone for some of the HCG monoclonal antibodies were determined. The high affinity and specificity of these monoclonal antibodies permit their clinical application in a sensitive sandwich solid-phase enzyme-linked and radioimmunoassay of HCG.","PeriodicalId":14978,"journal":{"name":"Journal of applied biochemistry","volume":"6 1-2","pages":"48-55"},"PeriodicalIF":0.0,"publicationDate":"1984-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17548901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of human serum RNase activity by aurine tricarboxylic acid polymer: effect on the antigen-antibody reaction. 金三羧酸聚合物对人血清RNase活性的抑制:对抗原-抗体反应的影响。

Journal of applied biochemistry Pub Date : 1984-02-01

A Kumar, R Ali

引用次数: 0