Inhibition of human serum RNase activity by aurine tricarboxylic acid polymer: effect on the antigen-antibody reaction.

Abstract

The structural integrity of RNA as antigen in immunological reactions with serum as the source of antibody is endangered because of RNase activity. Aurine tricarboxylic acid (ATA) in its polymeric form has been used to overcome this problem by inhibiting serum RNase. At low concentrations, the polymer completely arrests the RNA degrading activity. An appreciable increase in antibody activity was observed with an anti-RNA serum pretreated with ATA polymer.