Lin Zhang, Min Yang, Dong Wang, Kaixin Yu, Zhuoyun Chen, Xue Bing, Peng Zhao, Yiqing Bao, Xuebo Yin
{"title":"Accurate determination of high sulfur content in sulfide samples: an optimized ICP-OES method","authors":"Lin Zhang, Min Yang, Dong Wang, Kaixin Yu, Zhuoyun Chen, Xue Bing, Peng Zhao, Yiqing Bao, Xuebo Yin","doi":"10.1186/s40543-024-00463-z","DOIUrl":"https://doi.org/10.1186/s40543-024-00463-z","url":null,"abstract":"Determination of high sulfur content in sulfide minerals is crucial for various applications, including geochemistry, mining, and environmental monitoring. While inductively coupled plasma optical emission spectrometry (ICP-OES) is the preferred method for sulfur analysis, conventional sample preparation techniques often fall short when dealing with high sulfur concentrations. This study investigated optimized digestion conditions for accurate sulfur determination in sulfide minerals using ICP-OES. We employed anti-corrosion acid digestion bombs, an aqua regia–hydrofluoric acid system, and a digestion temperature of 180 °C, achieving exceptional results. Certified reference material recoveries ranged from 99.9 to 100.5%, with relative standard deviations between 0.42 and 0.84%. These results demonstrate the high accuracy and precision of the method. The optimized digestion protocol presented in this study provides a robust and reliable approach for accurate sulfur analysis in sulfide minerals, particularly for high concentrations.","PeriodicalId":14967,"journal":{"name":"Journal of Analytical Science and Technology","volume":"8 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stefano Frassati, Elena Barbaro, Claudia Rossetti, Giulio Cozzi, Clara Turetta, Federico Scoto, Marco Roman, Matteo Feltracco, Kitae Kim, Carlo Barbante, Andrea Gambaro, Andrea Spolaor
{"title":"Inorganic iodine and bromine speciation in Arctic snow at picogram-per-grams levels by IC-ICP-MS","authors":"Stefano Frassati, Elena Barbaro, Claudia Rossetti, Giulio Cozzi, Clara Turetta, Federico Scoto, Marco Roman, Matteo Feltracco, Kitae Kim, Carlo Barbante, Andrea Gambaro, Andrea Spolaor","doi":"10.1186/s40543-024-00462-0","DOIUrl":"https://doi.org/10.1186/s40543-024-00462-0","url":null,"abstract":"Iodine and bromine play central roles in polar atmospheric chemistry: iodine influences the atmospheric oxidative capacity and can generate cloud condensation nuclei, while bromine participates in ozone depletion reactions, known as bromine explosions. Here we present a very sensitive analytical method for Br and I speciation by coupling the ion chromatography system (IC) with an inductively coupled plasma sector field mass spectrometer (ICP-SFMS). We achieved sub-picogram-per gram (pg g−1) as limits of detection (LODs) ranging from 0.4 pg g−1 for I−, 0.8 pg g−1 for IO3−, 4 pg g−1 for Br−, and 1 pg g−1 for BrO3−, respectively. These values represent a decrease of up to 30 times compared to the LODs reported in other studies. The method was validated using deep snow samples from the Svalbard Islands, collected at the end of the polar night to quantify various oxidized compounds during their seasonal minimum. In the future, this method could prove useful in the paleoclimatic study of ice cores and snow, as well as in ice chemistry research.","PeriodicalId":14967,"journal":{"name":"Journal of Analytical Science and Technology","volume":"20 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142252712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Min-Ji Jung, Youn-Joong Jeong, Woo-Jin Shin, Albert Chang-sik Cheong
{"title":"Isotopic distribution of bioavailable Sr, Nd, and Pb in Chungcheongbuk-do Province, Korea","authors":"Min-Ji Jung, Youn-Joong Jeong, Woo-Jin Shin, Albert Chang-sik Cheong","doi":"10.1186/s40543-024-00460-2","DOIUrl":"https://doi.org/10.1186/s40543-024-00460-2","url":null,"abstract":"Mapping the distribution of bioavailable isotope ratios across landscapes serves as an efficient geochemical tool for delineating the origins and migration trajectories of humans and animals. Chungcheongbuk-do Province in central Korea, known for its geological diversity and inland location isolated from coastal influences, provides an ideal area to study the contributions of geological and environmental factors to the isotope landscape (isoscape). This study analyzed the distribution of bioavailable Sr, Nd, and Pb isotopes in the province using plant and soil data obtained in this study and from previous works. Chungcheongbuk-do features diverse geological elements, including Precambrian basement, Paleozoic metasedimentary rocks, and Mesozoic granitoids and volcano-sedimentary sequences. The 87Sr/86Sr ratios of bulk soil samples from 44 sites primarily range from 0.781 to 0.706, with two ratios exceeding 0.9 originating from Precambrian basement and Cretaceous granitoid areas. Fractions of soils treated with 1 M ammonium nitrate and acetic acid exhibit indistinguishable 87Sr/86Sr ratios (R2 = 0.99, except for one point), spanning from 0.804 to 0.707. Plant 87Sr/86Sr ratios demonstrate a robust positive correlation with leachate ratios (for ammonium nitrate data, (87Sr/86Sr)plant = 0.938 × (87Sr/86Sr)leachate + 0.045, R2 = 0.98). The εNd values of bulk soils from Precambrian basement areas (–18 to –30) plot against Sm/Nd ratios around the reference line corresponding to 3 Ga, while other bulk soil samples (εNd = –1 to –21) align with a younger (~ 2 Ga) reference line. Plant εNd values, ranging from –4 to –24, exhibit a prominent positive correlation with ammonium nitrate leachates (plant εNd = 0.77 × leachate εNd – 3.83, R2 = 0.89). Plant samples do not show consistent variation between 87Sr/86Sr and εNd. The 206Pb/204Pb and 207Pb/204Pb ratios of bulk soils show a ~ 2 Ga trend, typical for Korean basement rocks. The Pb isotopic ratios of ammonium nitrate and acetic acid leachates match perfectly with each other (R2 = 0.99). The 206Pb/204Pb, 207Pb/204Pb, and 208Pb/204Pb ratios of plant samples vary narrowly (19.2–17.9; 15.8–15.5; 39.0–38.0) and are distinctly different from those of bulk soils (24.4–17.9; 16.5–15.6; 42.5–37.9) and their leachates (23.1–17.7; 16.2–15.6; 41.0–38.1). Plant and soil data from this study and previous works were used to construct Sr and Nd isoscapes, employing interpolation models based on inverse distance weighting, simple kriging, empirical Bayesian kriging, and geology and topography-considered empirical Bayesian kriging regression prediction. These maps await validation through analysis of additional archives. The isotope data obtained in this study highlight a strong geological control over bioavailable Sr and Nd, in contrast to a dominant environmental influence on bioavailable Pb. The Sr and Nd isoscapes presented here are potentially valuable for addressing archaeological or forensic inquiries in their current s","PeriodicalId":14967,"journal":{"name":"Journal of Analytical Science and Technology","volume":"30 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141948180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hung M. Vu, Ju Yeon Lee, Yongmin Kim, Sanghoon Park, Fabiana Izaguirre, Juhyeon Lee, Jung-Hyun Lee, Minjoung Jo, Hye Ryun Woo, Jin Young Kim, Pyung Ok Lim, Min-Sik Kim
{"title":"Exploring the feasibility of a single-protoplast proteomic analysis","authors":"Hung M. Vu, Ju Yeon Lee, Yongmin Kim, Sanghoon Park, Fabiana Izaguirre, Juhyeon Lee, Jung-Hyun Lee, Minjoung Jo, Hye Ryun Woo, Jin Young Kim, Pyung Ok Lim, Min-Sik Kim","doi":"10.1186/s40543-024-00457-x","DOIUrl":"https://doi.org/10.1186/s40543-024-00457-x","url":null,"abstract":"Recent advances in high-resolution mass spectrometry have now enabled the study of proteomes at the single-cell level, offering the potential to unveil novel aspects of cellular processes. Remarkably, there has been no prior attempt to investigate single-plant cell proteomes. In this study, we aimed to explore the feasibility of conducting a proteomic analysis on individual protoplasts. As a result, our analysis identified 978 proteins from the 180 protoplasts, aligning with well-known biological processes in plant leaves, such as photosynthetic electron transport in photosystem II. Employing the SCP package in the SCoPE2 workflow revealed a notable batch effect and extensive missing values in the data. Following correction, we observed the heterogeneity in single-protoplast proteome expression. Comparing the results of single-protoplast proteomics with those of bulk leaf proteomics, we noted that only a small fraction of bulk data was detected in the single-protoplast proteomics data, highlighting a technical limitation of the current single-cell proteomics method. In summary, we demonstrated the feasibility of conducting a single-protoplast proteomic experiment, revealing heterogeneity in plant cellular proteome expression. This underscores the importance of analyzing a substantial number of plant cells to discern statistically significant changes in plant cell proteomes upon perturbation such as abscisic acid treatment in future studies. We anticipate that our study will contribute to advancing single-protoplast proteomics in the near future.","PeriodicalId":14967,"journal":{"name":"Journal of Analytical Science and Technology","volume":"14 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141885735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative stagewise mapping of trace elements using ICP-OES in five Ayurvedic Marine Drugs highlights their posologic and clinical implications","authors":"Sandeep Bhagwan Chavan, Tanhaji Balu Walunj, Vidya Shrikant Gupta, Vineeta Vasant Deshmukh, Sadanand Prabhakar Sardeshmukh","doi":"10.1186/s40543-024-00458-w","DOIUrl":"https://doi.org/10.1186/s40543-024-00458-w","url":null,"abstract":"Rasashastra, an Ayurvedic pharmaceutical branch, scientifically converts natural resources into palatable therapeutic dosage forms. Ayurvedic Marine Drugs (AMD) are mainly calcium carbonate/ calcite, but in Ayurvedic treatment modality they are uniquely used for several ailments. Moreover, their detailed microchemical compositions have seldom been reported. In this study, five raw materials, conch, oyster shell, cowry, coral, and pearl, their transitions during the manufacturing process, and the final drugs were analyzed for identification and quantitation of minor elements using a validated inductively coupled plasma optical emission spectrophotometry method. All the five raw materials contained traces of Al, Ag, B, Ba, Cu, Co, Cr, Fe, Hg, Li, K, Mg, Mn, Na, Ni, Pb, S, Sr, Tl, and Zn. Interestingly, B, Co, Cr, Li, Mn, and Ni were absent in the finished products, whereas Pb and Hg exhibited a considerable decrement during the drug formation. The first three components of principal component analysis together explained 72.09% of variance. Raw AMD indicated positive factor scores, whereas their respective purified and incinerated forms exhibited negative scores. Cluster analysis derived a dendrogram typically forming incinerated AMD group but not for the raw forms. Coral with Mg as highest content was a remarkable outlier in both these analyses as compared to Na in others. In discriminant analysis of the presence/absence of elements and periodic table-based classifications, coral and pearl showed higher variation indicating as discriminants when equated with others. Periodic table-based classification was a better model for discrimination. Correlation analysis in both classifications revealed a strong correlation of conch with oyster shells and vice versa, cowry and pearl with oyster shell and a weak correlation of coral with pearl. Coral was a good predictor followed by conch, pearl, oyster shell, and cowry, sequentially, and can be considered a moderately sensitive but highly specific model. Thus, ICP-OES technique is highly precise and accurate for assessing the micro-composition of raw, in-process, and finished products to ensure their transitions, quality, and genuineness. Such characterized traditional medicines should be further investigated in depth for their mechanistic aspects. ","PeriodicalId":14967,"journal":{"name":"Journal of Analytical Science and Technology","volume":"219 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141885737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dokyung Kwon, Yongju Jo, Youngku Sohn, Jeongkwon Kim
{"title":"Gaseous ozone inactivation of Bacillus atrophaeus spores on ceramic and porcelain tiles","authors":"Dokyung Kwon, Yongju Jo, Youngku Sohn, Jeongkwon Kim","doi":"10.1186/s40543-024-00456-y","DOIUrl":"https://doi.org/10.1186/s40543-024-00456-y","url":null,"abstract":"In this study, we investigated the ozone inactivation efficiency of Bacillus atrophaeus spores attached to various tile surfaces. Eight different types of tiles were employed, considering factors such as porosity (ceramic, porcelain), color (white, black), and glossiness (matte, glossy). Inactivation was performed by exposing the spore-loaded tiles to ozone gas for a specified duration. The inactivation efficiencies of ozone gas on different tile surfaces were compared by analyzing the colony-forming units of desorbed Bacillus atrophaeus cultured in a growth medium. Results revealed a reduction in colony counts with increasing ozone exposure time, indicating a proportional enhancement in inactivation effectiveness on ozone exposure time. After exposure to ozone gas for 30 min or longer, more than 90% of spores on each tile were inactivated. Regarding porosity, ceramic tiles exhibited slightly superior inactivation effects compared to porcelain tiles. Additionally, in terms of glossiness, glossy tiles demonstrated better inactivation effects than matte tiles. However, no significant differences were observed in inactivation effects based on the color of the tiles.","PeriodicalId":14967,"journal":{"name":"Journal of Analytical Science and Technology","volume":"63 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141885736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jangil Moon, Yeongcheol Han, Songyi Kim, Jeonghoon Lee, Soon Do Hur
{"title":"Integrating the controlled evaporation mixer with cavity ring-down spectroscopy for enhanced water vapor isotope calibration","authors":"Jangil Moon, Yeongcheol Han, Songyi Kim, Jeonghoon Lee, Soon Do Hur","doi":"10.1186/s40543-024-00455-z","DOIUrl":"https://doi.org/10.1186/s40543-024-00455-z","url":null,"abstract":"Accurate measurement of water vapor isotopes (δ18O and δ2H) is fundamental for advancing our understanding of the hydrological cycle and improving hydrological model accuracy. This study introduces an innovative calibration methodology using a controlled evaporation mixer (CEM) for determining stable isotopic ratios in atmospheric water vapor via cavity ring-down spectroscopy. The CEM technique reliably produces a stable water vapor stream, crucial for enhancing the precision and accuracy of isotopic measurements. Its rapid adaptation to changes in water vapor concentration and compatibility with different water standards enhance calibration reliability. Demonstrated reproducibility in generating water vapor across a broad concentration range from 900 to over 25,000 ppmv, coupled with a substantial reduction in memory effects, makes this approach highly effective in both laboratory and field settings. This calibration advancement greatly enhances research capabilities for continuous atmospheric water vapor analysis, providing deeper insights into hydrological processes and atmospheric dynamics.","PeriodicalId":14967,"journal":{"name":"Journal of Analytical Science and Technology","volume":"3 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141569997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khadiga M. Kelani, Yasmin Mohammed Fayez, Asmaa G. Gad, Amr M. Mahmoud
{"title":"Design of point-of-care electrochemical sensor for therapeutic drug monitoring of ofloxacin in biological fluids","authors":"Khadiga M. Kelani, Yasmin Mohammed Fayez, Asmaa G. Gad, Amr M. Mahmoud","doi":"10.1186/s40543-024-00450-4","DOIUrl":"https://doi.org/10.1186/s40543-024-00450-4","url":null,"abstract":"A disposable screen-printed sensor has been crafted specifically for therapeutic drug monitoring purposes, particularly for detecting ofloxacin in biological fluids. To enhance selectivity toward ofloxacin, a supramolecular calix [6] arene serves as the ionophore of choice. The sensor incorporates a graphene nanocomposite as an ion-to-electron transducer layer, which not only boosts potential stability but also mitigates potential drift. The developed ofloxacin sensor underwent rigorous characterization following IUPAC guidelines. The linearity range spans from 1 × 10–6 to 1 × 10–2 M, with a measured slope of 59.0 mV/decade. Impressively, it boasts a percentage recovery of 100.18 ± 1.60 and a low detection limit (LOD) of 6 × 10–7 M. Stability assessments indicate reliable performance over an extended period of 8 weeks. The versatility of this sensor extends to various applications, including the determination of ofloxacin in pharmaceutical formulations, bulk powder, and biological fluids. Notably, it has demonstrated efficacy post-bioanalysis validation, adhering to Food and Drug Administration regulations. This advancement holds promise for personalized therapeutic drug monitoring in clinical pharmacy studies and quality control laboratories, thereby optimizing patient care at the point-of-care. ","PeriodicalId":14967,"journal":{"name":"Journal of Analytical Science and Technology","volume":"17 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141551122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exonuclease III assisted exponential amplification reaction (EXPAR) for specific miRNA-155 analysis during post-anesthetic nursing","authors":"Xuejun Wu, Shaolan Zou, Jingshen Dai","doi":"10.1186/s40543-024-00454-0","DOIUrl":"https://doi.org/10.1186/s40543-024-00454-0","url":null,"abstract":"The persistent obstacle in precise and sensitive identification of microRNAs (miRNAs) pertains to the advancement of expeditious and effective isothermal amplification methodologies suitable for point-of-care environments and monitoring the cancer prognosis in patients receiving post-anesthetic nursing. The exponential amplification reaction (EXPAR) has attracted considerable interest due to its simplicity and ability to rapidly amplify signals. The practical application of the EXPAR is, nevertheless, severely hampered by the inability to differentiate closely related homologous sequences and to modify the designed templates to suit other targets. A loop-stem template for the EXPAR system was developed in this study to facilitate specific target recognition with the aid of exonuclease III (Exo III). This innovation effectively eliminated non-specific hybridization that could occur between the template and interfering sequences, thereby ensuring minimal background amplification of EXPAR. By modulating Exo III-based target recycling, EXPAR based chain amplification and G4/hemin based color reaction, this method facilitated the precise and sensitive examination of miRNA-155, yielding acceptable yields and a minimal detection limit of 0.43 fM. The approach expedites simple and expeditious molecular diagnostic applications involving short nucleic acids and offers an innovative method for enhancing the selectivity of EXPAR-based techniques, providing a robust tool for monitoring the expression level from patients receiving post-anesthetic nursing and guiding the treatment strategy.","PeriodicalId":14967,"journal":{"name":"Journal of Analytical Science and Technology","volume":"32 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141529292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hyosuk Yun, Jeong-Eun Seon, Kon-Young Ji, Hye Jung Min, Hyung-Sik Kang, Chul Won Lee
{"title":"NMR structure and functional studies of the fourth FAS1 domain of human periostin","authors":"Hyosuk Yun, Jeong-Eun Seon, Kon-Young Ji, Hye Jung Min, Hyung-Sik Kang, Chul Won Lee","doi":"10.1186/s40543-024-00453-1","DOIUrl":"https://doi.org/10.1186/s40543-024-00453-1","url":null,"abstract":"Periostin is a matricellular protein that consists of several structural and functional domains, including EMILIN-like, four internal repeat fasciclin1 (FAS1) domains, and a carboxyl-terminal variable domain. It is known that periostin is associated with various fundamental biological processes and diseases, such as several types of cancer and chronic inflammatory diseases. Despite its important roles, the biological function of each domain is poorly understood. In this study, we expressed the fourth FAS1 (FAS1 IV) domain of human periostin, which was highly soluble and stable enough for structural and functional studies. The three-dimensional structure of FAS1 IV was determined using 3D NMR spectroscopy. The overall structure of FAS1 IV consists of six α-helices, one 310 helix, and eight β-strands. Two triangular α-helical modules formed by three α-helices each are located on one side of the molecule, while the orthogonal β-sheet sandwich module of FAS1 IV is located on the other side. The isolated FAS1 IV domain exhibited cell invasion, migration, and adhesion activities for cancer cell lines comparable to those of the full FAS1 I–IV domain. In conclusion, we propose that the FAS1 IV domain is functionally active in human periostin and provides valuable information for understanding the biological function of periostin.","PeriodicalId":14967,"journal":{"name":"Journal of Analytical Science and Technology","volume":"25 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141529293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}