{"title":"Effects of Chinese Herbal Formulas on Gut Microflora to Juvenile Chinese Softshell Turtles Pelodiscus sinensis Wiegmann","authors":"Mingsong Xiao, Dandan Ju, Huimin Xu, Bing Gong","doi":"10.1155/jai/9064272","DOIUrl":"https://doi.org/10.1155/jai/9064272","url":null,"abstract":"<p>As a natural “green” feed additive, Chinese herbal medicines (CHMs) are extensively used in aquaculture. Nevertheless, little information is obtainable regarding the performance of CHMs on the composition of gastrointestinal microbiota in healthy juvenile <i>Pelodiscus sinensis</i>. Here, we investigated the intestinal microbial community composition of <i>P</i>. <i>sinensis</i> fed with artificial feed added with CHM feeding additive. Three hundred juvenile <i>P</i>. <i>sinensis</i> (3.92 ± 0.08 g) were randomly divided into 5 groups in quadruplicate. 0.0%, 1.0%, 2.0%, 3.0%, and 4.0% of CHMs were added to the control group (CG), test group 1 (TG1), test group 2 (TG2), test group 3 (TG3), and test group 4 (TG4), respectively. After the aquaculture experiment was completed, the intestinal microbial community of <i>P</i>. <i>sinensis</i> was examined by 16S rDNA sequencing method. The results showed that the relative abundance of Proteobacteria and <i>Firmicutes</i> were the two most dominant phyla, and there were significant differences in TG1, TG2, TG3, and TG4 compared to the CG. The abundance of phylum Proteobacteria and genus <i>Citrobacter</i> increased with the increase of the dose of CHMs (<i>p</i> < 0.05), while a clear decrease in the relative abundance of phylum <i>Firmicutes</i> and genus <i>Romboutsia</i>, genus <i>unclassified_f_Enterobacteriaceae</i>, and genus <i>Clostridium_sensu_stricto_1</i> was observed in the CHMs groups (<i>p</i> < 0.05), which could be considered as the indicators of the medication due to CHMs as a feed additive modulating the gut microbiota. The results of this study indicated that dietary supplementation with 2.0% of CHM feeding additive could significantly improve the intestinal microbiota composition of juvenile <i>P</i>. <i>sinensis</i>.</p>","PeriodicalId":14894,"journal":{"name":"Journal of Applied Ichthyology","volume":"2026 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jai/9064272","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148012282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marjan Norouzi, Emel Armashi, Preeta Kochanian, Ali Shariari, Amirparviz Salati
{"title":"Effect of Food Deprivation and Refeeding on Antioxidant Defense Status in Asian Sea Bass (Lates calcarifer)","authors":"Marjan Norouzi, Emel Armashi, Preeta Kochanian, Ali Shariari, Amirparviz Salati","doi":"10.1155/jai/1149239","DOIUrl":"https://doi.org/10.1155/jai/1149239","url":null,"abstract":"<p>This study examined the impact of short-term starvation on oxidative stress and antioxidant defense mechanisms in juvenile Asian sea bass (<i>Lates calcarifer</i>). Juvenile fish (35 ± 9 g) were randomly allocated to 12 300 L fiberglass tanks (15 fish/tank) following acclimation. Experimental treatments (in triplicate) consisted of starvation periods of 2, 4, and 8 days, each followed by 16 days of refeeding, alongside a continuously fed control group. Fish were hand-fed a commercial diet to satiation twice daily. Liver and muscle tissues were sampled poststarvation and postrefeeding, following euthanasia with 2-phenoxyethanol. Growth performance; lipid peroxidation (malondialdehyde, MDA); and activities of key antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were assessed. Starvation significantly influenced weight gain, with the 8-day group exhibiting reduced growth compared to other treatments (<i>p</i> < 0.05). Antioxidant enzyme activities in muscle tissue remained unchanged across treatments; however, liver samples from the 8-day starvation group showed significant alterations in antioxidant indices (<i>p</i> < 0.05). These findings indicate that hepatic antioxidant defense is more responsive to starvation period than muscle tissue is and that starvation period of 8 days or longer may induce oxidative stress in the liver.</p>","PeriodicalId":14894,"journal":{"name":"Journal of Applied Ichthyology","volume":"2026 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jai/1149239","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147615012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Matthew J. Wilson, Sara A. Ashcraft, Daniel E. Ressler
{"title":"Length–Weight Relationships for 22 Mid-Atlantic Species Show Variability in Slopes Spatially, Temporally, and With Published Regressions","authors":"Matthew J. Wilson, Sara A. Ashcraft, Daniel E. Ressler","doi":"10.1155/jai/6481233","DOIUrl":"https://doi.org/10.1155/jai/6481233","url":null,"abstract":"<p>Despite their importance in understanding stream food webs and community dynamics, small-bodied and nongame fish remain relatively understudied. This is also true of the basic ecology for these taxa, such as calculating length–weight relationships (LWRs). Here we present LWRs for 22 species including nongame and small-bodied taxa from eastern North America in the Susquehanna River watershed in > 30 small watersheds across four years, seven families, and > 7500 individuals. To contextualize these LWRs, we compared the slopes (<i>b</i>) of our relationships for abundant species across broad-scale watersheds, over time, and to published slopes in eastern North America. We found significant variability in <i>b</i> for our four most abundant species: <i>Rhinichthys atratulus</i> (blacknose dace), <i>Semotilus atromaculatus</i> (creek chub), <i>R. cataractae</i> (longnose dace), and <i>Etheostoma olmstedi</i> (tessellated darter) across both watershed and year of sampling yet inconsistent changes across species. We also found published values for <i>b</i> to be > 0.1 different from our LWRs for 36% of species, indicating wide variability across their geographic range as well. Collectively, these results highlight variability in LWRs, and while they may caution against the generalized use for management, they also identify opportunities for LWR use as a tool for comparing populations. The addition of these equations to the literature fills an important gap in our understanding of LWRs for eastern North American fishes, while also providing regional, temporal, and taxonomic context for consistency of LWRs across these species.</p>","PeriodicalId":14894,"journal":{"name":"Journal of Applied Ichthyology","volume":"2026 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jai/6481233","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147568488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Shaping the Future of Aquaculture in Kuwait: Expansion, Diversification, and Sustainability","authors":"Haya AlQuood, Abdulrahman Ben-Hasan","doi":"10.1155/jai/2443814","DOIUrl":"https://doi.org/10.1155/jai/2443814","url":null,"abstract":"<p>Aquaculture accounts for around 50% of global seafood production, with its contribution expected to expand significantly in the coming decade. While countries in the Arabian Gulf region are leveraging aquaculture to meet rising seafood demand and enhance food security, Kuwait’s sector remains largely underdeveloped. In this paper, challenges and opportunities for aquaculture intensification in Kuwait are identified, emphasizing the need for the development and intensification of farmed species and the adoption of sustainable farming systems. By examining case studies, existing aquaculture endeavors, and current regulatory frameworks, a roadmap is proposed for the sustainable expansion of Kuwait’s aquaculture industry, which underscores the biological, economic, and regulatory dimensions. Recommendations include suitable species and systems for farming; considerations for local supply chain production; and ramping up aquaculture production while diversifying farmed species and farming systems will be critical in the future of Kuwait’s seafood security.</p>","PeriodicalId":14894,"journal":{"name":"Journal of Applied Ichthyology","volume":"2026 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jai/2443814","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147565795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Davide Bottacini, Piotr Fromentin, Inge Franse, Alexander Kotrschal
{"title":"First Record of the Yellowbar Angelfish, Pomacanthus maculosus (Forsskål, 1775) (Teleostei, Pomacanthidae) in Greek Waters","authors":"Davide Bottacini, Piotr Fromentin, Inge Franse, Alexander Kotrschal","doi":"10.1155/jai/4587058","DOIUrl":"https://doi.org/10.1155/jai/4587058","url":null,"abstract":"<p>We report the first sighting of the yellowbar angelfish <i>Pomacanthus maculosus</i> (Forsskål, 1775) (Teleostei, Pomacanthidae) in Greece. We observed a young adult multiple times in the summer of 2025 over the course of 10 weeks on a rocky pinnacle on the island of Crete. Identification was based on visual underwater observations and analysis of video material. <i>P. maculosus</i> is a coral reef fish native to the western Indian Ocean. We suggest that the individual reached the Mediterranean through the Suez Canal, possibly aided by the intense ship traffic around our study location.</p>","PeriodicalId":14894,"journal":{"name":"Journal of Applied Ichthyology","volume":"2026 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jai/4587058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147565268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correction to “Length-Weight Relationships of Sixteen Fish Species From the Upper Tietê Basin, Southeaster Brazil”","authors":"","doi":"10.1155/jai/9876456","DOIUrl":"https://doi.org/10.1155/jai/9876456","url":null,"abstract":"<p>L. G. Nogueira de Carvalho, B. C. Gazzola Prieto, C. S. Bento, et al., “Length-Weight Relationships of Sixteen Fish Species From the Upper Tietê Basin, Southeaster Brazil,” <i>Journal of Applied Ichthyology</i>, 2025, 3200000, https://doi.org/10.1155/jai/3200000.</p><p>In the article, the following statement was erroneously omitted from the Acknowledgements section:</p><p>“This study was financed, in part, by the São Paulo Research Foundation (FAPESP), Brasil. Process Number #2025/18372-9, São Paulo Research Foundation (FAPESP)”</p><p>We apologize for this error.</p>","PeriodicalId":14894,"journal":{"name":"Journal of Applied Ichthyology","volume":"2026 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jai/9876456","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147562782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammad Saddam Hussain, Mahadevan Harikrishnan, Betsee Thomas, P. V. Keerthana, A. R. Nikhila Khanna, Haritha Haridas, Dinesh Yadav
{"title":"Oocyte Development and Fecundity Strategy in Cage-Reared Pearlspot, Etroplus suratensis (Cichlidae): A Histological and Statistical Approach","authors":"Mohammad Saddam Hussain, Mahadevan Harikrishnan, Betsee Thomas, P. V. Keerthana, A. R. Nikhila Khanna, Haritha Haridas, Dinesh Yadav","doi":"10.1155/jai/7923916","DOIUrl":"https://doi.org/10.1155/jai/7923916","url":null,"abstract":"<p>The progression of oocyte development and patterns of oocyte recruitment are key indicators of reproductive strategy in fishes. The present study examines, for the first time, the fecundity strategy of cage-reared <i>Etroplus suratensis</i> in Vembanad Lake using four indicators: (a) the oocyte size frequency distribution across ovarian reproductive phases and sampling months; (b) the proportional distribution of the oocyte developmental stages across each ovarian reproductive phase; (c) seasonal trends in the mean diameter of advanced vitellogenic oocytes; and (d) the seasonal incidence of oocyte atresia. The samples were collected monthly from October 2023 to September 2024, with 202 ovaries categorized by macroscopic observation into various ovarian reproductive phases. From these, 54 ovaries representing all ovarian reproductive phases were selected for histological examination and image analysis techniques. Histological examination identified seven distinct oocyte development stages in addition to postovulatory follicles and atretic oocytes. The oocyte size distribution revealed asynchronous oocyte development and continuous secondary growth, with no distinct separation between previtellogenic and vitellogenic oocytes. Gaussian mixture model analysis identified five distinct oocyte size clusters corresponding to successive developmental stages, confirming the presence of multiple cohorts. Logistic regression showed that the size at which 50% of oocytes recruited into vitellogenesis decreased across spawning-capable phases, reflecting rapid oocyte recruitment during repeated spawning events. A seasonal decline in the mean diameter of advanced vitellogenic oocytes was evident during the spawning season, indicating the continuous replenishment of oocytes within the advanced vitellogenic pool. A relatively stable proportion of cortical alveolar and vitellogenic oocytes during the spawning season suggests continuous recruitment of oocytes into the vitellogenic pool through <i>de novo vitellogenesis</i>. Furthermore, an increased occurrence of atretic oocytes was noticeable towards the end of the spawning season (i.e., August to October), reflecting a common strategy in species with indeterminate fecundity to reabsorb surplus oocytes. These findings demonstrate that <i>E. suratensis</i> exhibits asynchronous oocyte development, continuous oocyte recruitment and batch spawning with an indeterminate fecundity pattern. This reproductive strategy observed supports prolonged spawning activity and provides important implications for brood stock management and seed production in estuarine cage culture systems.</p>","PeriodicalId":14894,"journal":{"name":"Journal of Applied Ichthyology","volume":"2026 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/jai/7923916","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147569278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}