Journal of Applied Genetics最新文献_第8页

Usefulness of alien sterilizing cytoplasms for the hybrid breeding of triticale (xTriticosecale Wittmack): preliminary results. 外来灭菌细胞质对杂交育种三尖杉（xTriticosecale Wittmack）的用途：初步结果。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-05-01 Epub Date: 2024-06-14 DOI: 10.1007/s13353-024-00882-z

Magdalena Simlat, Tomasz Warzecha, Stefan Stojałowski, Halina Góral

{"title":"Usefulness of alien sterilizing cytoplasms for the hybrid breeding of triticale (xTriticosecale Wittmack): preliminary results.","authors":"Magdalena Simlat, Tomasz Warzecha, Stefan Stojałowski, Halina Góral","doi":"10.1007/s13353-024-00882-z","DOIUrl":"10.1007/s13353-024-00882-z","url":null,"abstract":"To be useful for cereal breeding, cytoplasmic male sterility (CMS) should express the complete sterility of maternal lines and the full restoration of the male fertility of F1 hybrids. The most reliable source of sterilizing cytoplasm for triticale is Triticum timopheevi; however, due to the low frequency of efficient non-restorer genotypes for this cytoplasm, new sources of CMS are needed. In this study, aside from T. timopheevi (T) cytoplasm, three alternative CMS sources were tested: Pampa (P) from Secale cereale L., Aegilops sharonensis (A), and Ae. ventricosa (V). The suitability of these cytoplasms for breeding was assessed based on the male fertility/sterility of F1 hybrids obtained through the manual pollination of CMS maternal lines with 36 triticale cultivars and breeding strains. About half of the hybrids with each type of cytoplasm were fully fertile and produced more than 30 grains per bagged spike. The highest percentage was found in hybrids with P cytoplasm (58.33%) and the lowest in hybrids with A cytoplasm (44.44%). Male sterility was observed in hybrids with P cytoplasm (16.67%) and A cytoplasm (16.67%) but not in hybrids with T or V cytoplasm. In terms of practical aspects, male sterility systems with P or A cytoplasm exhibit similarity in their ability to restore male fertility that differ from the T and V cytoplasms. Although all studied cytoplasms exhibited some disadvantages for breeding purposes, none should be definitively classified as unacceptable for future breeding programs regarding the development of triticale hybrid cultivars.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"305-310"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12000179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141317392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphic insertions of DcSto miniature inverted-repeat transposable elements reveal genetic diversity structure within the cultivated carrot. DcSto微型倒装重复转座元件的多态插入揭示了栽培胡萝卜的遗传多样性结构。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-05-01 Epub Date: 2024-10-28 DOI: 10.1007/s13353-024-00916-6

Santosh Hadagali, Katarzyna Stelmach-Wityk, Alicja Macko-Podgórni, Sarvamangala Cholin, Dariusz Grzebelus

{"title":"Polymorphic insertions of DcSto miniature inverted-repeat transposable elements reveal genetic diversity structure within the cultivated carrot.","authors":"Santosh Hadagali, Katarzyna Stelmach-Wityk, Alicja Macko-Podgórni, Sarvamangala Cholin, Dariusz Grzebelus","doi":"10.1007/s13353-024-00916-6","DOIUrl":"10.1007/s13353-024-00916-6","url":null,"abstract":"Miniature inverted-repeat transposable elements (MITEs) are a potent source of polymorphisms in plant genomes. A genotyping system, named DcS-ILP, based on polymorphic insertions of Stowaway MITEs (DcStos) localized in introns and identified in the reference genome DH1, has been developed for carrot. Here, we report an extension of the DcS-ILP genotyping system by incorporation of non-reference insertions identified in resequenced genomes representing the eastern gene pool. We genotyped 52 carrot accessions representing the eastern and western carrot gene pools with 92 markers developed previously (western DcS-ILP panel) together with 84 newly developed markers (eastern DcS-ILP panel). Overall, the DcS-ILP markers revealed a highly structured genetic diversity separating the eastern and the western carrot accessions at K = 2 and differentiating Indian breeding lines from the eastern accessions at K = 3. The eastern DcS-ILP panel proved to be more robust with respect to the eastern carrot gene pool, while it provided little information on the western accessions, as many of the DcSto insertions present in the eastern gene pool were absent in the western gene pool. As the western carrot accessions represent improved cultivars, DcSto insertional polymorphisms allowed detection of a selection-driven bottleneck at the improvement stage. Selection in the course of the improvement stage generally operated on standing variation, as the subset of DcSto insertions present in the western carrot likely originated from transposition events preceding the separation of both gene pools. However, occasional frequency shifts in the opposite direction were also revealed, possibly indicating selection for favorable variants associated with DcSto insertions.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"293-303"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12000206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of core genes and intercellular communication role in osteosarcoma. 发现骨肉瘤的核心基因和细胞间通信作用。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-05-01 Epub Date: 2024-05-30 DOI: 10.1007/s13353-024-00872-1

Fanyu Meng, Xinshe Zhou, Zhi Zhao, Lijia Pei, Weiguo Xia

{"title":"Discovery of core genes and intercellular communication role in osteosarcoma.","authors":"Fanyu Meng, Xinshe Zhou, Zhi Zhao, Lijia Pei, Weiguo Xia","doi":"10.1007/s13353-024-00872-1","DOIUrl":"10.1007/s13353-024-00872-1","url":null,"abstract":"Osteosarcoma is a primary malignant bone tumor that affects children and young adults. Understanding the molecular mechanisms underlying osteosarcoma is critical to develop effective treatments. This study aimed to identify core genes and explore the role of intercellular communication in osteosarcoma. We used GSE87437 and GSE152048 dataset to conduct a weighted correlation network analysis (WGCNA) and identify co-expression modules. The enriched biological processes and cellular components of the genes in the steelblue module were analyzed. Next, we explored the expression, diagnostic value, correlation, and association with immune infiltrate of CCSER1 and LOC101929154. Finally, we utilized CIBERSORT algorithm to predict the infiltrated immune cells in osteosarcoma tissues. Our results identified 44 co-expression modules, and the steelblue module was mainly associated with axon development, axonogenesis, and innervation. CCSER1 and LOC101929154 were significantly upregulated in osteosarcoma tissues with poor response to preoperative chemotherapy. Moreover, the expressions of CCSER1 and LOC101929154 were positively correlated. The area under the receiver operating characteristic curve of CCSER1 and LOC101929154 was 0.800 and 0.773, respectively. The expression of CCSER1 was negatively correlated with follicular helper T cells and positively correlated with M0 macrophages, while LOC101929154 was negatively correlated with activated mast cells. Besides, CD4 memory-activated T cells were observed at lower levels in patients who responded well to chemotherapy. Our study identified core genes CCSER1 and LOC101929154 and provided insight into the intercellular communication profile in osteosarcoma. Our results suggested that targeting CCSER1, LOC101929154, and CD4 memory-activated T cells may be a promising strategy for the treatment of osteosarcoma.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"323-332"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulatory effects of in ovo delivery of galactooligosaccharide and Lactiplantibacillus plantarum on antioxidant capacity, gene expression, and selected plasma metabolite parameters of broiler chickens. 半乳糖低聚糖和植物乳杆菌对肉仔鸡抗氧化能力、基因表达和选定血浆代谢参数的调节作用

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-05-01 Epub Date: 2024-12-12 DOI: 10.1007/s13353-024-00931-7

Modou Mangan, Katarzyna Połtowicz, Cornelia C Metges, Maria Siwek

{"title":"Modulatory effects of in ovo delivery of galactooligosaccharide and Lactiplantibacillus plantarum on antioxidant capacity, gene expression, and selected plasma metabolite parameters of broiler chickens.","authors":"Modou Mangan, Katarzyna Połtowicz, Cornelia C Metges, Maria Siwek","doi":"10.1007/s13353-024-00931-7","DOIUrl":"10.1007/s13353-024-00931-7","url":null,"abstract":"A stable gut microbiota promotes a healthy gut and enhances immune function, antioxidant status, and metabolic activities in chickens. The present research work aimed to investigate the modulatory impacts of in ovo delivery of prebiotic and probiotic on oxidative stress, the intestinal transcriptome, and various plasma metabolites in chickens. Fertilized Ross 308 eggs were administered in ovo either with galactooligosaccharide (GOS) (3.5 mg/egg or Lactiplantibacillus plantarum (LP) 1 × 106/egg on the 12th day of egg incubation. Three hundred viable Ross 308 broiler hatching eggs in total were randomly assigned to four groups, namely, the negative control not injected group, the group receiving physiological saline injections as the positive control, GOS, and LP. The analysis of genes associated with immune functions, antioxidants, barrier functions, and free fatty acid receptors were determined via qPCR. The analysis of the selected plasma blood metabolites was performed automatically with Pentra C 400. The antioxidant capacity of the chickens' liver, breast muscle, and spleen was enhanced by the in ovo injection of GOS and LP. The immune-related gene expression levels were upregulated after in ovo stimulation with either GOS or LP which improved the gut health of broiler chickens. In addition, several genes related to gut barrier functions were upregulated, thus ensuring epithelial integrity. As for blood plasma metabolites, no adverse effects were observed. In summary, we report that in ovo stimulation with either GOS or LP stimulates the immune system and improves the antioxidant status and gut health of chickens with no negative impact on plasma blood metabolite indices.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"421-434"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12000254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endometrial factors and pregnancy loss frequency in recurrent pregnancy loss patients: comparing RT-PCR microbiology, microbial cultures, and immunohistochemistry of endometrium biopsy. 复发性妊娠丢失患者子宫内膜因素与妊娠丢失频率：子宫内膜活检RT-PCR微生物学、微生物培养和免疫组化的比较

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-05-01 Epub Date: 2025-02-20 DOI: 10.1007/s13353-025-00949-5

K Klimaszyk, P Wirstlein, K Bednarek-Rajewska, M Jankowski, H Svarre Nielsen, E Wender Ożegowska, M Kędzia

{"title":"Endometrial factors and pregnancy loss frequency in recurrent pregnancy loss patients: comparing RT-PCR microbiology, microbial cultures, and immunohistochemistry of endometrium biopsy.","authors":"K Klimaszyk, P Wirstlein, K Bednarek-Rajewska, M Jankowski, H Svarre Nielsen, E Wender Ożegowska, M Kędzia","doi":"10.1007/s13353-025-00949-5","DOIUrl":"10.1007/s13353-025-00949-5","url":null,"abstract":"The objective of this study is to investigate the presence of bacteria in endometrial samples from patients with recurrent pregnancy loss (RPL) and explore potential correlations between bacterial presence, chronic endometritis, and previous pregnancy loss history. Endometrial samples from 90 RPL patients were analysed using RT-PCR to detect 10 specific bacterial species. A subgroup of 65 patients underwent additional microbial culture and immunohistochemistry for plasma cell identification. Correlations between bacterial presence, chronic endometritis, and the number of previous pregnancy losses were evaluated. We detected at least one out of 10 chosen bacteria DNA by RT-PCR in 24.4% (22/90) of endometrial samples. Patients with PCR-identified bacteria had a significantly higher number of previous pregnancy losses (median 3 vs 2, p = 0.01). No correlation was observed between bacterial presence and chronic endometritis diagnosis. A significant correlation was found between bacterial detection by PCR and microbial culture (p = 0.03), though culture methods detected fewer positive cases. In RPL patients, detecting DNA from at least one of 10 selected bacterial species by RT-PCR correlates with a higher number of previous pregnancy losses. However, this bacterial presence does not correlate with chronic endometritis diagnosis based on the CD138 immunohistochemistry-identified plasma cell count. These findings suggest a potential role of endometrial bacteria in RPL that may be independent of the classical inflammatory response associated with chronic endometritis.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"459-468"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12000213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143457813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and molecular mechanisms underlying the male sterility in rice. 水稻雄性不育的遗传和分子机制。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-05-01 Epub Date: 2024-12-04 DOI: 10.1007/s13353-024-00923-7

Amir Sohail, Chengkai Lu, Peng Xu

引用次数: 0

Correction to: Unraveling the genetic basis of MODY: insights from next‑generation sequencing. 更正：揭示 MODY 的遗传基础：新一代测序的启示。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-05-01 DOI: 10.1007/s13353-024-00914-8

Metin Eser, Gulam Hekimoglu, Fatma Dursun

引用次数: 0

Expanding TBCE-related phenotype-novel variant causing rigid spine, eosinophilia, neutropenia, and nocturnal hypoxemia. TBCE相关表型的扩展--导致脊柱僵硬、嗜酸性粒细胞增多症、中性粒细胞减少症和夜间低氧血症的新型变体。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-05-01 Epub Date: 2024-08-17 DOI: 10.1007/s13353-024-00894-9

Magdalena Badura-Stronka, Adam Sebastian Hirschfeld, Evgenia Globa, Anna Winczewska-Wiktor, Anna Potulska-Chromik, Anna Kostera-Pruszczyk, Dorota Wicher, Maciej Robert Krawczyński

{"title":"Expanding TBCE-related phenotype-novel variant causing rigid spine, eosinophilia, neutropenia, and nocturnal hypoxemia.","authors":"Magdalena Badura-Stronka, Adam Sebastian Hirschfeld, Evgenia Globa, Anna Winczewska-Wiktor, Anna Potulska-Chromik, Anna Kostera-Pruszczyk, Dorota Wicher, Maciej Robert Krawczyński","doi":"10.1007/s13353-024-00894-9","DOIUrl":"10.1007/s13353-024-00894-9","url":null,"abstract":"We report three patients with the novel variant c.100 + 1G > A of the TBCE gene and describe the presented clinical phenotype in detail. We also systematically reviewed the literature for clinical similarities and dissimilarities among all known patients with pathogenic TBCE variants. The clinical phenotype observed in patients with pathogenic TBCE variants is broader than previously described. Homozygous carriers of the c.100 + 1G > A variant exhibit a markedly milder clinical course, with no deviations in the calcium-phosphate metabolism and central nervous system pathology in MRI studies. Additionally, two patients manifest highly specific symptoms such as a rigid spine, eosinophilia, neutropenia, and nocturnal hypoxemia. Furthermore, cryptorchidism was observed in male patients. The identification of the pathogenic c.100 + 1G > A variant has thus far been limited to patients of Central-Eastern European descent, suggesting a potential founder mutation in this population.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"363-373"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12000180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of doublesex and Mab-3 (DMRT) gene family in Ctenopharyngodon idella (grass carp). 草鱼双倍和 Mab-3 (DMRT) 基因家族的分子特征。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-05-01 Epub Date: 2024-11-28 DOI: 10.1007/s13353-024-00924-6

Shakeela Parveen, Muhammad Farhan Khan, Mehwish Sultana, Saif Ur Rehman, Laiba Shafique

{"title":"Molecular characterization of doublesex and Mab-3 (DMRT) gene family in Ctenopharyngodon idella (grass carp).","authors":"Shakeela Parveen, Muhammad Farhan Khan, Mehwish Sultana, Saif Ur Rehman, Laiba Shafique","doi":"10.1007/s13353-024-00924-6","DOIUrl":"10.1007/s13353-024-00924-6","url":null,"abstract":"Doublesex and Mab-3 (DMRT) gene family is a diverse group of transcriptional factors crucially involved in sex differentiation and biological processes such as body growth and differentiation in vertebrates. In this study, we analyzed DMRT genes structural characterization and physiochemical properties, and elucidated their functional role as a ligand of different gonadal receptors including androgen (AR), estrogen β (ER-β), estrogen γ (ER-γ), and progesterone (PR). All six genes of the DMRT gene family in grass carp (Ctenopharyngodon Idella Valenciennes, 1844) exhibited an acidic nature. These DMRT genes are primarily localized in the nucleus, where they play a role in DNA binding via doublesex DNA binding motif. All the DMRT gene pairs are under strong purifying selection with two segmental duplications envisaged about 18.30 (DMRT3a/DMRTA2) and 24.90 (DMRT2b/DMRT2a) million years ago (MYA). Recombination analysis revealed six potential recombinant breakpoints posing substantial evolutionary pressure for diverse cellular functioning of DMRT isoforms. Moreover, the DMRTA1 protein had a highest binding affinity of - 270.42 and - 267.16 for androgen receptors (AR) and progesterone receptors (PR), whereas, for estrogen receptors ER-β and ER-γ, the maximum binding affinity was observed with DMRT2a and DMRT2b proteins showing a docking score of - 254.22 and - 261.71, respectively. First time we studied the binding scores and interface residues of the DMRT genes as a ligand of gonadal receptors that play a crucial role in fish growth, sex development and differentiation, and spermatogenesis and oocyte maturation. The present study provides a molecular basis for DMRT genes in grass carp that may serve as a reference for in-depth phylogenomic study in other species.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"409-420"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hfq influences ciprofloxacin accumulation in Escherichia coli independently of ompC and ompF post-transcriptional regulation. Hfq对大肠杆菌中环丙沙星积累的影响独立于ompC和ompF转录后调控。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-05-01 Epub Date: 2025-02-08 DOI: 10.1007/s13353-025-00945-9

Florian Turbant, Natalia Lewandowska, Sylwia Bloch, Frank Wien, Hugo Chauvet, Grzegorz Węgrzyn, Véronique Arluison

{"title":"Hfq influences ciprofloxacin accumulation in Escherichia coli independently of ompC and ompF post-transcriptional regulation.","authors":"Florian Turbant, Natalia Lewandowska, Sylwia Bloch, Frank Wien, Hugo Chauvet, Grzegorz Węgrzyn, Véronique Arluison","doi":"10.1007/s13353-025-00945-9","DOIUrl":"10.1007/s13353-025-00945-9","url":null,"abstract":"The antibiotic resistance of pathogenic bacteria is currently one of the major problems in medicine, and finding novel antibacterial agents is one of the most difficult tasks in the field of biomedical sciences. Studies on such tasks can be successful only if genetic and molecular mechanisms leading to antibiotic resistance/sensitivity are understood. Previous reports indicated that the bacterial protein Hfq, discovered as an RNA chaperone but subsequently demonstrated to play also other functions in cells, is involved in the mechanisms of the response of bacterial cells to antibiotics. Recently, it was found that Hfq dysfunction resulted in more effective accumulation of an antibiotic ciprofloxacin in Escherichia coli cells irrespective of the presence or absence of the AcrB efflux pump. However, small RNA-mediated impairment of expression of the ompF gene, which encodes a porin involved in antibiotics influx, reversed the effects of the absence of Hfq on the antibiotic accumulation. This led to the hypothesis that Hfq might influence ciprofloxacin accumulation in the manner independent on its RNA chaperone function, as this protein might also influence cellular membrane structure and functions. Here, we demonstrate that in ompC and ompF mutants of E. coli, accumulation of ciprofloxacin is significantly impaired in the absence of Hfq or its C-terminal domain. These results corroborate the above-mentioned hypothesis on a sRNA-independent mechanism of Hfq-mediated modulation of the antibiotic transmembrane transport. Since fluoroquinolones use both protein- and lipid-mediated pathways to cross the outer membrane, Hfq may influence both processes. This possibility will be discussed herein.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"449-457"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143370604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0