Journal of Applied Genetics最新文献_第6页

Transcriptome analysis revealed SMURF2 as a prognostic biomarker for oral cancer. 转录组分析显示，SMURF2 是口腔癌的预后生物标志物。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-02-01 Epub Date: 2024-05-03 DOI: 10.1007/s13353-024-00869-w

Lu Deng, Zhihong Wu, Chuanxi Sun, Zhe Liu

{"title":"Transcriptome analysis revealed SMURF2 as a prognostic biomarker for oral cancer.","authors":"Lu Deng, Zhihong Wu, Chuanxi Sun, Zhe Liu","doi":"10.1007/s13353-024-00869-w","DOIUrl":"10.1007/s13353-024-00869-w","url":null,"abstract":"Background: The activation of TGF-β pathway can facilitate tumorigenesis. Understanding the TGF-related genes (TRGs) in oral cancer and determining their prognostic value is of utmost importance.Methods: The TRGs were selected to develop a prognostic model based on lasso regression. Oral cancer patients were classified into high-risk and low-risk groups based on the risk model. Subsequently, multivariate COX regression was employed to identify the prognostic marker. Additionally, the expression of SMURF2 was validated using quantitative real-time polymerase chain reaction (qRT-PCR) and the Human Protein Atlas (HPA) database. To investigate the relationship between SMURF2 expression and immune cell infiltrations, we conducted single-sample Gene Set Enrichment Analysis (ssGSEA) analyses.Results: We identified 16 differentially expressed TRGs in oral cancer, all of which showed upregulation. From these, we selected eight TRGs as prognostic signatures. Furthermore, the high-risk group demonstrated lower infiltration levels of immune cells, immune score, and higher tumor purity. Interestingly, we also found that SMURF2 serves as an independent prognostic biomarker. SMURF2 was upregulated in oral cancer, as confirmed by public databases and qRT-PCR analysis. Importantly, our results indicate a close association between SMURF2 expression and the immune microenvironment.Conclusion: The 8-TRG signature prognosis model that we constructed has the ability to predict the survival rate and immune activity of oral cancer patients. SMURF2 could be effective in recognizing prognosis and evaluating immune efficacy for oral cancer.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"155-170"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brown trout in Oder estuary tributaries: genetic structure, stocking, and admixture. 奥得河口支流中的褐鳟：遗传结构、放养和混合。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-02-01 Epub Date: 2024-07-03 DOI: 10.1007/s13353-024-00890-z

Rafał Bernaś, Anna Wąs-Barcz, Waldemar Święcki, Piotr Dębowski, Grzegorz Radtke, Adam Tański, Agata Korzelecka-Orkisz, Krzysztof Formicki

{"title":"Brown trout in Oder estuary tributaries: genetic structure, stocking, and admixture.","authors":"Rafał Bernaś, Anna Wąs-Barcz, Waldemar Święcki, Piotr Dębowski, Grzegorz Radtke, Adam Tański, Agata Korzelecka-Orkisz, Krzysztof Formicki","doi":"10.1007/s13353-024-00890-z","DOIUrl":"10.1007/s13353-024-00890-z","url":null,"abstract":"Several rivers that are tributaries of the Oder estuary are inhabited by Salmo trutta L, the most important of which are Ina, Gowienica, and Wołczenica. Both forms of the species, sea trout and resident brown trout, are present. All rivers are traditionally stocked with either sea trout from the neighboring Pomeranian river Rega basin or resident brown trout from various locations. To examine populations in these rivers in terms of genetic structure, genetic diversity, and origin, they were analyzed using 13 microsatellite loci. Relatedness was also assessed for fish stocked in the same year. The obtained genotypes were compared with breeding stocks used for stocking in Poland. The analyses revealed a significant genetic distance between adult individuals from Ina and Rega Rivers and fish caught during electrofishing. Strong kinship relationships were identified in the sampled areas, with high proportions of fish originating from stocking and their dominance in numbers over wild juveniles, primarily in smaller tributaries. Additionally, clear separation in the origin of stocked individuals was observed. Adult trout from Ina and Rega are genetically closer to northern brown trout lineages, providing crucial information for the management and biodiversity conservation of Polish Salmo trutta populations.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"183-193"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Giardia fibrillarin: a bioinformatics exploration of sequence and structure. 贾第虫纤维素：序列和结构的生物信息学探索。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-02-01 Epub Date: 2024-11-11 DOI: 10.1007/s13353-024-00920-w

Francisco Alejandro Lagunas-Rangel

{"title":"Giardia fibrillarin: a bioinformatics exploration of sequence and structure.","authors":"Francisco Alejandro Lagunas-Rangel","doi":"10.1007/s13353-024-00920-w","DOIUrl":"10.1007/s13353-024-00920-w","url":null,"abstract":"Fibrillarin is a key nucleolar S-adenosyl-L-methionine (SAM)-dependent methyltransferase, highly conserved among species in both sequence and function. It is actively involved in numerous cellular processes, particularly in the early stages of pre-ribosomal RNA processing. Although a fibrillarin orthologue has been identified in Giardia, an intestinal protozoan parasite responsible for numerous infections worldwide, its functional and structural features in this parasite remain largely unexplored. In this study, bioinformatics tools were used to analyze the sequence and structure of Giardia fibrillarin to provide insights that could aid future experimental and therapeutic investigations. Taking advantage of the protein sequence of Giardia fibrillarin, multiple bioinformatics tools were employed to estimate its domains, nuclear and nucleolar localization signals (NLS and NoLS), post-translational modifications, phylogeny, and three-dimensional structure. The analysis revealed significant conservation of Giardia fibrillarin, showing close relationships with archaeal and parasitic amoeba orthologs. It retains essential features such as the SAM-dependent methyltransferase domain, a glycine- and arginine-rich domain, and both NLS and NoLS. While the core region of the protein is structurally conserved, the N-terminal region exhibits notable divergence. The potential for inhibition of Giardia fibrillarin by SAM competitors suggests that it could be a promising target for drug development, particularly for strains resistant to current treatments.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"241-248"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HPV-driven oncogenesis-much more than the E6 and E7 oncoproteins. 人乳头瘤病毒驱动的肿瘤发生--远超过 E6 和 E7 肿瘤蛋白。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-02-01 Epub Date: 2024-06-22 DOI: 10.1007/s13353-024-00883-y

J Janiszewska, M Kostrzewska-Poczekaj, M Wierzbicka, J C Brenner, M Giefing

引用次数: 0

What, how, and why? - anti-EHEC phages and their application potential in medicine and food industry. 是什么、如何以及为什么？- 抗 EHEC 噬菌体及其在医药和食品工业中的应用潜力。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-02-01 Epub Date: 2024-11-11 DOI: 10.1007/s13353-024-00918-4

Agnieszka Necel, Aleksandra Dydecka, Gracja Topka-Bielecka, Wojciech Wesołowski, Natalia Lewandowska, Sylwia Bloch, Bożena Nejman-Faleńczyk

{"title":"What, how, and why? - anti-EHEC phages and their application potential in medicine and food industry.","authors":"Agnieszka Necel, Aleksandra Dydecka, Gracja Topka-Bielecka, Wojciech Wesołowski, Natalia Lewandowska, Sylwia Bloch, Bożena Nejman-Faleńczyk","doi":"10.1007/s13353-024-00918-4","DOIUrl":"10.1007/s13353-024-00918-4","url":null,"abstract":"Enterohemorrhagic Escherichia coli (EHEC) are pathogens that, only in the United States, cause more than 250,000 foodborne infections a year. Since antibiotics or other antidiarrheal agents may increase the hemolytic-uremic syndrome (HUS) development risk, currently only supportive therapy, including hydration, is used. Therefore, many methods to fight EHEC bacteria focus on their use in food processing to prevent human infection. One of the proposed anti-EHEC agents is bacteriophages, known for their bactericidal effect, host specificity, and lack of cross-resistance with antibiotics. In this review article, we provide an overview of the characteristics like source of isolation, morphology, kinetics of life cycle, and treatment potential of over 130 bacteriophages able to infect EHEC strains. Based on the reviewed literature, we conclude that bacteriophages may play a highly significant role in regulating EHEC propagation. In addition, we also point out the phage features that should be taken into account not only when using bacteriophages but also when examining their properties. This may contribute to accelerating the pace of work on the preventive use of bacteriophages, which is extremely needed in the modern world of the food industry, but also stimulate interest in phages and accelerate regulatory work that would enable the use of bacteriophages also in medicine, to fight the drug-resistant strains.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"219-240"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and validation of a novel nine-gene prognostic signature of stem cell characteristic in hepatocellular carcinoma. 肝癌干细胞特征的新型九基因预后特征的鉴定与验证

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-02-01 Epub Date: 2024-03-05 DOI: 10.1007/s13353-024-00850-7

Yahang An, Weifeng Liu, Yanhui Yang, Zhijie Chu, Junjun Sun

{"title":"Identification and validation of a novel nine-gene prognostic signature of stem cell characteristic in hepatocellular carcinoma.","authors":"Yahang An, Weifeng Liu, Yanhui Yang, Zhijie Chu, Junjun Sun","doi":"10.1007/s13353-024-00850-7","DOIUrl":"10.1007/s13353-024-00850-7","url":null,"abstract":"Currently, cancer stem cells (CSCs) are regarded as the most promising target for cancer therapy due to their close association with tumor resistance, invasion, and recurrence. Thus, identifying CSCs-related genes and constructing a prognostic risk model associated with CSCs may be crucial for hepatocellular carcinoma (HCC) therapy. Xena Browser was used to download gene expression profiles and clinical data, while MSigDB was used to obtain genes associated with CSCs. Firstly, the non-negative matrix factorization (NMF) algorithm was used to cluster the HCC samples based on CSCs-related genes. To evaluate the predictive performance of the risk model, the receiver operating characteristic curves (ROC) and Kaplan-Meier analysis were used. The R package \"rms\" was used to construct the final nomogram based on risk scores and clinical characteristics. Based on 449 CSCs-related genes, a total of 588 HCC samples from TCGA-LIHC and ICGC-LIRI_JP were classified into four molecular subtypes with marked differences in survival and mRNA stemness index (mRNAsi) between subtypes. Univariate Cox regression, multivariate Cox regression, and LASSO regression analyses were performed on a total of 1417 differentially expressed genes (DEGs) between subtypes, and a nine-gene prognostic model was constructed with TTK, ST6GALNAC4, SPP1, SGCB, MEP1A, HTRA1, CD79A, C6, and ATP2A3. In both the training and testing sets and the external validation cohort, the risk model performed well in predicting HCC patients' survival. A nomogram was constructed and had high predictive efficacy in short-term survival. In comparison with the other two prognostic models, our nine-gene signature model performed best. We constructed a nine-gene signature model to predict the survival of HCC patients, which has good predictive efficacy and stability. The model may contribute to guiding the prognostic assessment of HCC patients in clinical practice.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"127-140"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140028110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome profiling and metabolic pathway analysis towards reliable biomarker discovery in early-stage lung cancer. 转录组图谱分析和代谢途径分析有助于发现早期肺癌的可靠生物标记物。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-02-01 Epub Date: 2024-03-05 DOI: 10.1007/s13353-024-00847-2

Muthu Kumar Thirunavukkarasu, Priyanka Ramesh, Ramanathan Karuppasamy, Shanthi Veerappapillai

{"title":"Transcriptome profiling and metabolic pathway analysis towards reliable biomarker discovery in early-stage lung cancer.","authors":"Muthu Kumar Thirunavukkarasu, Priyanka Ramesh, Ramanathan Karuppasamy, Shanthi Veerappapillai","doi":"10.1007/s13353-024-00847-2","DOIUrl":"10.1007/s13353-024-00847-2","url":null,"abstract":"Earlier diagnosis of lung cancer is crucial for reducing mortality and morbidity in high-risk patients. Liquid biopsy is a critical technique for detecting the cancer earlier and tracking the treatment outcomes. However, noninvasive biomarkers are desperately needed due to the lack of therapeutic sensitivity and early-stage diagnosis. Therefore, we have utilized transcriptomic profiling of early-stage lung cancer patients to discover promising biomarkers and their associated metabolic functions. Initially, PCA highlights the diversity level of gene expression in three stages of lung cancer samples. We have identified two major clusters consisting of highly variant genes among the three stages. Further, a total of 7742, 6611, and 643 genes were identified as DGE for stages I-III respectively. Topological analysis of the protein-protein interaction network resulted in seven candidate biomarkers such as JUN, LYN, PTK2, UBC, HSP90AA1, TP53, and UBB cumulatively for the three stages of lung cancers. Gene enrichment and KEGG pathway analyses aid in the comprehension of pathway mechanisms and regulation of identified hub genes in lung cancer. Importantly, the medial survival rates up to ~ 70 months were identified for hub genes during the Kaplan-Meier survival analysis. Moreover, the hub genes displayed the significance of risk factors during gene expression analysis using TIMER2.0 analysis. Therefore, we have reason that these biomarkers may serve as a prospective targeting candidate with higher treatment efficacy in early-stage lung cancer patients.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"115-126"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140039406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of nutritional composition in opaque2- and crtRB1-based single- and double-biofortified super sweet corn. 基于不透明2和crtRB1的单一和双重生物强化超级甜玉米的营养成分分析。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-02-01 Epub Date: 2024-05-11 DOI: 10.1007/s13353-024-00873-0

Bhavna Singh, Vignesh Muthusamy, Smriti Shrivastava, Gulab Chand, Nisrita Gain, Vinay Bhatt, Rajkumar U Zunjare, Firoz Hossain

{"title":"Analysis of nutritional composition in opaque2- and crtRB1-based single- and double-biofortified super sweet corn.","authors":"Bhavna Singh, Vignesh Muthusamy, Smriti Shrivastava, Gulab Chand, Nisrita Gain, Vinay Bhatt, Rajkumar U Zunjare, Firoz Hossain","doi":"10.1007/s13353-024-00873-0","DOIUrl":"10.1007/s13353-024-00873-0","url":null,"abstract":"Sweet corn has emerged as a favorite food item worldwide owing to its kernel sweetness. However, traditional sweet corn cultivars are poor in provitamin-A (proA) and essential amino acids, viz., lysine and tryptophan. So far, no sweet corn hybrid with high nutritional qualities has been commercialized elsewhere. Here, we analyzed accumulation of provitamin-A (proA), lysine, and tryptophan in a set of mutant versions of (i) crtRB1-, (ii) o2-, and (iii) crtRB1 + o2-based sweet corn inbreds and hybrids with (iv) traditional sweet corn (wild-type: O2 + CrtRB1). The crtRB1- and crtRB1 + o2-based genotypes possessed significantly higher proA (17.31 ppm) over traditional sweet corn (2.83 ppm), while o2- and crtRB1 + o2-based genotypes possessed significantly higher lysine (0.345%) and tryptophan (0.080%) over traditional sweet corn (lysine 0.169%, tryptophan 0.036%). Late sowing favored high kernel lysine, proA, and green cob yield among hybrids. Sweetness (17.87%) among the improved inbreds and hybrids was comparable to the original sweetcorn genotypes (17.84%). Among the four genotypic classes, crtRB1 + o2-based improved genotypes showed stronger association among traits over genotypes with o2 and crtRB1 genes alone. Significant association was observed among (i) proA and BC (r = 0.99), (ii) proA and BCX (r = 0.93), (iii) lysine and tryptophan (r = 0.99), and (iv) green cob yield with fodder yield (r = 0.73) in sweet corn hybrids. The study demonstrated that combining crtRB1 and o2 genes did not pose any negative impact on nutritional, yield, and agronomic performance. Sweet corn with crtRB1 + o2 assumes significance for alleviating malnutrition through sustainable and cost-effective approach.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"1-14"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140908623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing cytotoxicity and endoplasmic reticulum stress in human blood-brain barrier cells due to silver and copper oxide nanoparticles. 评估纳米银和纳米氧化铜对人血脑屏障细胞的细胞毒性和内质网应激反应。

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-02-01 Epub Date: 2024-02-09 DOI: 10.1007/s13353-024-00833-8

Luiza Chojnacka-Puchta, Dorota Sawicka, Lidia Zapor, Katarzyna Miranowicz-Dzierzawska

{"title":"Assessing cytotoxicity and endoplasmic reticulum stress in human blood-brain barrier cells due to silver and copper oxide nanoparticles.","authors":"Luiza Chojnacka-Puchta, Dorota Sawicka, Lidia Zapor, Katarzyna Miranowicz-Dzierzawska","doi":"10.1007/s13353-024-00833-8","DOIUrl":"10.1007/s13353-024-00833-8","url":null,"abstract":"In recent years, it has been generally accepted that metal-based nanoparticles (NPs) may induce stress in the endoplasmic reticulum (ER), a key organelle where protein folding occurs. We examined ER stress in immortalized human cerebral microvascular cells (hCMEC/D3) after exposure to silver-NPs (Ag-NPs)- and copper oxide-NPs (CuO-NPs) induced toxicity at < 10 nm and < 40 nm or < 50 nm diameters, respectively. In cytotoxicity assessments, cells were exposed to different CuO-NPs (5-400 µg/mL) or Ag-NPs (1-10 µg/mL) concentration ranges for 24 h and 72 h, and tetrazole salt reduction assays (EZ4U) were performed. Also, Ag-NP or CuO-NP effects on cell proliferation, apoptosis (caspase 3/7 assays), and ER stress and cell morphology were evaluated. In ER stress assessments, RNA-like endoplasmic reticulum kinase (PERK), activating transcription factor 6 (ATF6), inositol-requiring enzyme 1 (IRE1a), and others stress factor mRNA levels were determined after 24 h treatment using Real-Time PCR. Increased stress sensors (IRE1a, PERK, and ATF6) mRNA levels were observed after exposure to Ag-NPs (< 10 and < 40 nm) or CuO-NPs (< 50 nm). We investigated the expression of tight junction (TJ) proteins (barrier junctions) and showed that both types of NP reduced of OCLN gene expression. Morphological changes were observed after Ag-NP or CuO-NP exposure using holotomographic microscopy. Our data suggest that Ag- and CuO-NPs should undergo future in vitro and in vivo toxicology studies, especially for downstream biomedical application and occupational risk assessments.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"87-103"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139706775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of gene expression in immune-related organs by in ovo stimulation with probiotics and prophybiotics in broiler chickens. 肉鸡体内益生菌和预防性药物对免疫相关器官基因表达的调节作用

IF 2 3区生物学

Journal of Applied Genetics Pub Date : 2025-02-01 Epub Date: 2024-07-11 DOI: 10.1007/s13353-024-00891-y

Ramesha N Wishna-Kadawarage, Katarzyna Połtowicz, Rita M Hickey, Maria Siwek

{"title":"Modulation of gene expression in immune-related organs by in ovo stimulation with probiotics and prophybiotics in broiler chickens.","authors":"Ramesha N Wishna-Kadawarage, Katarzyna Połtowicz, Rita M Hickey, Maria Siwek","doi":"10.1007/s13353-024-00891-y","DOIUrl":"10.1007/s13353-024-00891-y","url":null,"abstract":"In ovo stimulation has been studied intensively as an alternative to antibiotic use in poultry production. We investigated the potential use of a probiotic in combination with a phytobiotic as a prophybiotic for in ovo stimulation and reported its beneficial effects on the gut microbiome of broiler chickens. The current study further investigates the gene expression in the immune-related organs of these chickens to understand the tissue-specific immunomodulatory effects of the treatments. The selected prophybiotic (Leuconostoc mesenteroides with garlic aqueous extract) and its probiotic component alone were injected into ROSS308 chicken eggs on the 12th day of incubation, and gene expression in cecal tonsils, spleen, and liver at 35 days of age was determined using qPCR method. The relative expression of each treatment was compared to the positive control, chickens injected with physiological saline in ovo. The results displayed a downregulation of pro- and anti-inflammatory cytokines in the cecal tonsils of the probiotic group and the liver of the prophybiotic group. The spleen displayed upregulated AVBD1 in both groups and upregulated IL1-β in the probiotic group. The probiotic group displayed increased expression of genes related to metabolism of energy (COX16), protein (mTOR), and lipids (CYP46A1) whereas the prophybiotic group displayed reduced expression of genes related to cholesterol synthesis (SREBP1) and glucose transportation (SLC2A2) in the liver. In conclusion, Leuconostoc mesenteroides differentially modulated gene expression in chickens when administered in ovo in combination with garlic aqueous extract. Further in ovo studies with different prophybiotic combinations are required to optimize the benefits in broiler chickens.","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"195-205"},"PeriodicalIF":2.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0