Journal of Applied Genetics最新文献

{"title":"Strigolactones modulate jasmonate-dependent transcriptional reprogramming during wound signalling in Arabidopsis.","authors":"Marek Marzec","doi":"10.1007/s13353-025-01005-y","DOIUrl":"https://doi.org/10.1007/s13353-025-01005-y","url":null,"abstract":"<p><p>Mechanical wounding triggers rapid transcriptional and hormonal reprogramming in plants, primarily driven by jasmonate (JA) signalling. While the role of JA, ethylene, and salicylic acid in wound responses is well characterised, the contribution of strigolactones (SLs) remains largely unexplored. Here, for the first time, it was shown that SLs modulate wound-induced transcriptional dynamics in Arabidopsis thaliana. Using transcriptome profiling of wild-type (Columbia-0) and the SL biosynthesis mutant more axillary growth3 (max3), a discrete cohort of genes whose wound induction is SL-dependent was identified. These genes include core JA biosynthetic genes and several JA-responsive transcriptional repressors, indicating that SLs potentiate early JA signalling. Promoter motif and protein-protein interaction analyses revealed that SLs regulate a transcriptional module composed of AP2/ERF, WRKY, and C2H2 zinc-finger factors, which integrate JA signalling, ROS homeostasis, and tissue regeneration. Notably, many of these factors are misregulated in max3 even prior to wounding, suggesting a primed but hypo-responsive state. Presented findings suggest a model in which SLs act upstream of the JA burst, coordinating transcriptional readiness and post-injury activation. This expands the functional scope of SLs in stress response and positions them as potential modulators of hormone crosstalk during wound responses.</p>","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scope for a threshold animal model for genetic evaluation for hip dysplasia.","authors":"Fabiana Michelsen de Andrade, Luiza Pinto Coelho Ribeiro Jardim, Darilene Ursula Tyska, Rodrigo Leivas Nunes, Jaime Araújo Cobuci","doi":"10.1007/s13353-025-00946-8","DOIUrl":"10.1007/s13353-025-00946-8","url":null,"abstract":"<p><p>Hip dysplasia (HD) is a major welfare problem, and in the present work, we investigated a sample of Bernese Mountain Dog breed bred in Brazil to estimate the genetic parameters for HD and to determine the best model for the estimation of breeding values (EBV) of dogs. The pedigree database consisted of 2218 dogs, of which 1202 had the hip phenotype available, corresponding to the X-ray (XR) classification according to the Fédération Cynologique Intenationale. Comparisons were made between seven different threshold models considering the HD phenotype with five classifications (reports from A to E) according to the XR (PHD1) and the binary phenotype with joints considered normal (report A) and abnormal (reports B to E) (PHD2). The threshold animal model was used to estimate the variance components and predict the genetic values. Fourteen models were evaluated, seven using variable PHD1 and another seven considering variable PHD2. Evaluated fixed effects were sex, birth year, or country of birth, which differed between models. The best fit model for the PHD1 phenotype estimated a heritability value of 0.30, while the best fit model for PHD2 estimated a value of 0.36. EBVs ranged from - 0.736 to 1.040 for PHD1 and from - 1.050 to 1.459 for PHD2, showing a wide diversity in the genetic merit of dogs. Approximately 40% of the animals had an EBV accuracy value greater than 50%. Considering the best two fit models for the tested phenotypes, we opted for the indication and use of the model working with PHD1, because of the direct correspondence of the PHD1 phenotype with the five possibilities of hip XR reports, once this facilitates understanding by both veterinarians and breeders. This model is used as the only fixed effect of the birth year. The present study is one of the first on canine genetic improvement approach in Brazil, and our results indicate the importance and potential of the use of EBV in the selection of sires for the improvement of HD in the national breeding of the breed.</p>","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"697-708"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A homozygous LAMB3 frameshift variant in junctional epidermolysis bullosa-affected Bleu du Maine sheep.","authors":"Anna Letko, Liesbeth Harkema, Karianne Peterson, Reinie Dijkman, Cord Drögemüller","doi":"10.1007/s13353-025-00957-5","DOIUrl":"10.1007/s13353-025-00957-5","url":null,"abstract":"<p><p>Epidermolysis bullosa (EB) is a group of inherited skin disorders characterized by skin fragility and blistering. Here, four Bleu du Maine lambs, sired by one ram, were diagnosed with EB very early in life. Due to the severity of the clinical signs, the lambs had to be euthanized. The affected lambs exhibited hoof sloughing and multiple ulcerations on the head, oral cavity, skin over the joints, and the ruminal pillars. Histopathology showed abrupt subepidermal clefts, epidermal detachment directly above the basal membrane, and ulcerations consistent with junctional EB (JEB). Two cases underwent whole-genome sequencing (WGS) to identify the genetic cause. Genomic analyses with the hypothesis of autosomal recessive inheritance identified the most likely pathogenic homozygous 1-bp deletion in the LAMB3 gene (NC_056065.1:g.73166198delG). Recessive forms of JEB in humans and dogs are caused by variants in LAMB3 gene, which encodes β3 subunit of laminin 332, a critical component of the epidermal basal membrane. The ovine frameshift variant putatively introduces a premature stop codon and disrupts the donor splice site of exon 20. The variant allele was homozygous in both sequenced cases and heterozygous in three unaffected close relatives and was absent in 1075 unrelated control sheep of various other breeds. This study highlights the importance of genetic investigation in veterinary diagnostics of and represents the first report of a LAMB3-related recessive EB in sheep. The findings enable genetic testing to inform breeding strategies and provide a second spontaneous large animal model for LAMB3-related JEB in humans.</p>","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"709-714"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urofacial (Ochoa) syndrome with a founder pathogenic variant in the HPSE2 gene: a case report and mutation origin.","authors":"Manuela Del Valle-Peréz, Alejandro Mejía-García, Dayana Echeverri-López, Katherine Gallo-Bonilla, Johanna A Tejada-Moreno, Andrés Villegas-Lanau, Mateo Chvatal-Medina, Jorge E Restrepo, Gina Cuartas-Montoya, Wildeman Zapata-Builes","doi":"10.1007/s13353-024-00896-7","DOIUrl":"10.1007/s13353-024-00896-7","url":null,"abstract":"<p><p>Urofacial syndrome or Ochoa syndrome (UFS or UFOS) is a rare disease characterized by inverted facial expression and bladder dysfunction that was described for the first time in Colombia. It is an autosomal recessive pathology with mutations in the HPSE2 and LRIG2 genes. However, 16% of patients do not have any mutations associated with the syndrome. Despite the importance of neurobiology in its pathophysiology, there are no neurological, neuropsychological, or psychological studies in these patients. A 30-year-old male from Medellín, Colombia, with a significant perinatal history, was diagnosed with grade 4 hydronephrosis on his first ultrasound test. At 4 months of age, symptoms such as hypomimia, lagophthalmos, and recurrent urinary tract infections started to manifest. Imaging studies revealed urinary tract dilatation, vesicoureteral reflux, and a double collector system on his left side, which led to the diagnosis of UFS. Multiple procedures, including vesicostomy, ureterostomy, and enterocystoplasty, were performed. At 20 years of age, he achieved urinary sphincter control. Genetic analysis revealed a founder pathogenic variant, c.1516C > T (p.Arg506Ter), in the HPSE2 gene, which produces a truncated protein that lacks 86 amino acids. This variant is classified as pathogenic according to the ClinVar database for UFS. The mutation age is approximately 260-360 years, and the two alleles share a 7.2-7.4 Mb IBD segment. Moreover, we detected European local ancestry in the IBD segment, which is consistent with a Spanish introduction. Neurological examination, neuropsychological assessment, and psychological testing revealed no abnormalities, except for high stress levels. Clinical analysis of this patient revealed distorted facial expression and detrusor-sphincter dyssynergia, which are typical of patients with UFS. Genetic analysis revealed a pathogenic variant in the HPSE2 gene of European origin and a mutation age of 260-360 years. From a neurological, neuropsychological, and psychological (emotional and personality) perspective, the patient showed no signs or symptoms of clinical interest.</p>","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"637-646"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Masquerading as lymphoma: the accelerated phase of Chediak-Higashi syndrome and its novel mutation.","authors":"Priyanka Aggarwal, Aditi Agarwal, Sonali Aggarwal, Deepa Rani, Vineeta Gupta","doi":"10.1007/s13353-025-00986-0","DOIUrl":"10.1007/s13353-025-00986-0","url":null,"abstract":"<p><p>Chediak-Higashi syndrome (CHS) is a rare autosomal recessive disorder. The clinical presentation may be fatal if these patients develop the catastrophic accelerated phase, i.e., hemophagocytic lymphohistiocytosis (HLH). We report a 2.5-year boy that presented to us with complaints of fever, recurrent cough, glandular neck swelling, and abdominal distension for 6 months. He also had a history of female sibling death (age, 3 years) 3 years ago with similar complaints. On examination, he had light skin and silver hair along with severe pallor, generalized significant lymphadenopathy, severe acute malnutrition, and hepatosplenomegaly. Since the patient's peripheral blood smear and bone marrow showed giant primary azurophilic granules in lymphocytes and eosinophils and the presence of 5 out of 8 HLH 2004 criteria, i.e., fever, hepatosplenomegaly, pancytopenia, hyperferritinemia, and hypertriglyceridemia, a diagnosis of CHS with HLH was made. However, no hemophagocytosis was observed. A novel homozygous nonsense variant in exon 45 of the LYST gene (chr1:g.235702929G > A) similar to the one found in the elder female sibling and previously reported \"likely pathogenic\" was discovered, which was identified through genetic testing. This case highlights the importance of genetic testing in diagnosis as well as antenatal counselling.</p>","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"653-656"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity and selection signatures in sheep breeds.","authors":"Julia Lisboa Rodrigues, Larissa Graciano Braga, Rafael Nakamura Watanabe, Flávio Schramm Schenkel, Donagh Pearse Berry, Marcos Eli Buzanskas, Danísio Prado Munari","doi":"10.1007/s13353-025-00941-z","DOIUrl":"10.1007/s13353-025-00941-z","url":null,"abstract":"<p><p>Natural and artificial selection in domesticated animals can cause specific changes in genomic regions known as selection signatures. Our study used the integrated haplotype score (iHS) and Tajima's D tests within non-overlapping windows of 100 kb to identify selection signatures, in addition to genetic diversity and linkage disequilibrium estimates in 9498 sheep from breeds in Ireland (Belclare, Charollais, Suffolk, Texel, and Vendeen). The mean observed and expected heterozygosity for all the sheep breeds were 0.353 and 0.355, respectively. Suffolk had the least genetic variation and, along with Texel, had slower linkage disequilibrium decay. iHS and Tajima's D detected selection signatures for all breeds, with some regions overlapping, thus forming longer segments of selection signatures. Common selection signatures were identified across iHS and Tajima's D methods for all breeds, with Belclare and Texel having several common regions under positive selection. Several genes were detected within the selection signature regions, including ITGA4, TLR3, and TGFB2 related to the immune system against endoparasites; DLG1, ROBO2, MXI1, MTMR2, CEP57, and FAM78B related to reproductive traits; WDR70 related to milk traits; SCHM1 and MYH15 related to meat traits; and TAS2R4, TAS2R39, and TAS2R40 related to adaptive traits. In conclusion, our results demonstrated moderate genetic diversity in the sheep breeds and detected and characterized selection signatures harboring genes associated with reproductive traits, milk production, meat production, and adaptive traits such as endoparasite resistance.</p>","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"675-687"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a novel gene expression panel for the characterization of MSCs for increased biological safety.","authors":"Anna M Różycka-Baczyńska, Igor M Stepaniec, Marta Warzycha, Izabela Zdolińska-Malinowska, Tomasz Oldak, Natalia Rozwadowska, Tomasz J Kolanowski","doi":"10.1007/s13353-024-00917-5","DOIUrl":"10.1007/s13353-024-00917-5","url":null,"abstract":"<p><p>Mesenchymal stromal cells (MSCs) have a wide range of therapeutic applications due to their multipotency, immunomodulatory, and anti-inflammatory properties. Their ability to migrate and recolonize damaged tissues is also remarkable. However, the controversial occurrence of spontaneous tumorigenesis or malignant transformation of MSCs raises concerns about proposed cell-based therapies for patients that researchers must address. There are several in vitro and in vivo strategies for MSC safety approval, but there is still no described coherent scheme that allows the assessment of MSC oncogenic potential in a simple, robust, and reproducible manner. Here, we have developed a diagnostic panel of molecular markers that allows for the accurate verification of the quality and safety of MSCs. Moreover, presented in this article diagnostic panel that can define the origin and tumorigenicity of MSCs can be easily introduced into the routine quality control processes of MSC-based product manufacturing which will improve further clinical applications of MSCs.</p>","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"623-636"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endophytic fungal communities of Calicotome spinosa-an important medicinal plant of Tizi-Ouzou (Algeria).","authors":"Amina Zareb, Piotr Banachewicz, Polina Havrysh, Lidia Błaszczyk, Tinhinane Hammad, Cylia Meftah, Sylwia Salamon","doi":"10.1007/s13353-025-00980-6","DOIUrl":"10.1007/s13353-025-00980-6","url":null,"abstract":"<p><p>Endophytic fungi enhance plant growth and improve tolerance to abiotic and biotic stress. Calicotome spinosa is an important medicinal plant that occupies the area of Tizi-Ouzou and is considered a nonflammable plant that maintains this ecosystem and regenerates degraded substrates. However, the knowledge about the endogenous fungal assembly of C. spinosa remains underexplored. Therefore, this study aimed to isolate and determine the fungal communities inhabiting the C. spinosa leaves endosphere using morphological and molecular identification methods. Morphological identification revealed the presence of Alternaria, Cladosporium, Mucor, Neoscytalidium, Rhizoctonia, Rhodotorula, Phoma, Penicillium, and Trichophyton genera. Molecular analysis of the selected phylogenetic markers' nucleotide sequences detailed the previous one and discovered the following: Biscogniauxia mediterranea, Schizophyllum commune, Penicillium polonicum, Athelia bombacina, Chaetomium globosum, Penicillium brevicompactum, Coriolopsis sp., Coprinellus sp., Aspergillus chevalieri, Aspergillus wentii, Canariomyces microspores, Alternaria alternata, Canariomyces notabilis, Rosellinia sp., Penicillium sp., and Alternaria sp. The taxonomic knowledge gained here for the first time about the group of endophytic fungi associated with the ethnomedicinal plant growing in Algeria, and the disposition of these fungi isolates provides a basis for further research into understanding the functions they may have in the plant. These isolates are therefore a source of potential candidates for further research into their possible applications as beneficial microorganisms in ecology, environmental and plant protection, and sustainable agriculture.</p>","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"763-769"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin in crop plants: from biosynthesis through pleiotropic effects to enhanced stress resilience.","authors":"Martyna Michałek, Piotr Ogrodowicz, Michał Kempa, Anetta Kuczyńska, Krzysztof Mikołajczak","doi":"10.1007/s13353-025-00963-7","DOIUrl":"10.1007/s13353-025-00963-7","url":null,"abstract":"<p><p>Melatonin plays a crucial role in enhancing plant resilience to environmental stresses by regulating physiological and biochemical responses. This review provides an overview of melatonin biosynthesis, signaling pathways, and its interactions with phytohormones, highlighting its multifunctional roles across various crop species. We summarize recent discoveries regarding the biosynthetic pathways of melatonin and its crucial metabolites, emphasizing the importance of tryptophan and serotonin in this process. Furthermore, we discuss the intricate crosstalk between melatonin and phytohormones, particularly auxins, cytokinins, and brassinosteroids, which collectively influence root development, growth, and stress tolerance, among other traits. The antioxidant activity of melatonin and its derivatives, along with their impact on photosynthesis, has also been thoroughly discussed. Notably, melatonin's regulatory actions promote root growth, thereby improving water and nutrient absorption under stress conditions. The identification of candidate genes and a putative receptor provides a foundation for future studies aimed at elucidating the molecular mechanisms underlying melatonin signaling in crop species. Ultimately, this review underscores the potential of harnessing melatonin in crop improvement strategies to enhance resilience to abiotic stresses while promoting sustainable agricultural practices.</p>","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"471-491"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12367889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico approach on structural and functional characterization of heat shock protein from Sulfobacillus acidophilus.","authors":"Pritish Mitra, Sabyasachi Chatterjee","doi":"10.1007/s13353-025-00964-6","DOIUrl":"10.1007/s13353-025-00964-6","url":null,"abstract":"<p><p>The 70 kDa heat shock proteins (Hsp70 s) are highly conserved and ubiquitous molecular chaperones. Hsp70 proteins are intimately involved in different biological activities including maintaining protein homeostasis and resisting environmental stress for survival. Characterizations of eukaryotic Hsp70 s with diverse functions are well established but investigations needed for prokaryotes. For better understanding, the sequences of Sulfobacillus acidophilus were retrieved from UniProt. Retrieved stress proteins were renamed as SaHsp70 s and performed an in silico analysis to identify sequential, structural properties and functional attributes. The in silico characterization of these proteins revealed that they are acidic, mostly thermostable globular protein with NAD(P)-binding Rossmann-folding. Molecular mass of SaHsp70 s ranged from 31.9 to 68.5 kDa and mainly localized in the cytoplasm. Phylogeny revealed the evolutionary distance and relationship among retrieved proteins. Domain analyzed only SaHsp70 - 1, SaHsp70 - 3, and SaHsp70 - 14 have actual conserved domain for Hsp70 and share the same clade on phylogenetic tree. Major part of each protein was abundant with α-helix and random coil which make it thermally stable and suitable for interacting with other proteins. SAVES and ProSA server proves the reliability, stability, and consistency tertiary structure of SaHsp70 s. Functional analysis was done in terms of membrane protein topology, PPI network generation, active and proteolytic cleavage sites prediction, conserved motif and domain detection. CastP predicted Gly, Lys, Thr, Glu, Pro, Gln, Arg and Val act as catalytic residue, are important for metal ions binding. Intramolecular interaction analysis suggested Lys67, Thr12, Thr170, Gly 168, Gly 169, and Glu 141 of SaHsp70 - 14 proteins could play central role in various complex cellular functions like stress mitigation, thermal stability, and related developmental processes.</p>","PeriodicalId":14891,"journal":{"name":"Journal of Applied Genetics","volume":" ","pages":"715-736"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}