Fizza Laraib, Rahmeen Rauf, Rimsha Dilshad, Nazia Jameel, R. Batool
{"title":"Characterization of Halophilic Bacteria Isolated from Khewra Salt Mines","authors":"Fizza Laraib, Rahmeen Rauf, Rimsha Dilshad, Nazia Jameel, R. Batool","doi":"10.54692/lgujls.2022.0602214","DOIUrl":"https://doi.org/10.54692/lgujls.2022.0602214","url":null,"abstract":"Halophilic bacteria can populate every niche of earth. These halophiles have a great potential of exopolysaccharides production that is of considerable importance in various industries. To screen EPS producing halophiles, saline soil samples were collected from Khewra salt mines, Jehlum, Pakistan. Twenty-two morphologically different bacterial strains were isolated by serial dilution method. All strains were considered as moderate halophilic bacteria as they could grow at 3-15% of NaCl concentration whereas only three strains could grow at 15% of NaCl, which belonged to the genus Bacillus and Pseudomonas. For screening of EPS production, P-medium was used. While for the estimation of slime production, congo-red agar was used that exhibited positive results by appearance of black colored colonies by many strains. Moreover, EPS production was analyzed quantitatively and qualitatively. Isolated Staphylococcus and Bacillus species produced high amount of EPS (20g/L). “Moderate halophiles” play an important role in therapeutics, bioremediation, food and medicine, petroleum and tanning industries by producing EPS. Recently, growth of many agriculture crops has been improved by using beneficial halophiles in saline soils. Consequently, with the help of these beneficial halophiles we can give benefit to mankind","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129338779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Updated Status of Indian Pangolin in South East Asia","authors":"B. N. Khan, Mubarra Ahsan, Yusra Ashfaq","doi":"10.54692/lgujls.2022.0602217","DOIUrl":"https://doi.org/10.54692/lgujls.2022.0602217","url":null,"abstract":"Indian Pangolin is the least studied mammal out of the 8 pangolin species. Since 2011, it is at risk of extinction because of illegal poaching for its scales and meat by local poor community and hunters. Its scales are used for medicines as well as making ornaments and meat is regarded as delicacy in different countries like China and India. Indian pangolin is economically very important as it serves as insect eater as well as provides shelter to other animals and aerates soil through borrowing. According to IUCN, it is regarded as ENDANGERED and will decline by about 50 percent over the next 20 years. So in order to save this species, there is need to aware people about its status at global level. In this article, past 10 years researches considering illegal trading of Indian pangolin in Asia have been reviewed.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124324857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Zahra, A. Imran, M. Saeed, K. Arif, Q. Syed, Syed Hussain Imam Abdi
{"title":"Edible Cutlery: A Sustainable Alternative to Plastic Cutlery for a Better Future","authors":"N. Zahra, A. Imran, M. Saeed, K. Arif, Q. Syed, Syed Hussain Imam Abdi","doi":"10.54692/lgujls.2022.0602213","DOIUrl":"https://doi.org/10.54692/lgujls.2022.0602213","url":null,"abstract":"Edible cutlery is an excellent concept to reduce the use of plastic cutlery and to save human health from the harmful effects of plastic utensils. Surveys revealed that only a negligible portion of the total plastic waste is recycled. The rest is added to the environment and contribute to pollution. Each year tonnes of plastic cutleries are dumped in the oceans and other water bodies which is affecting the life in water. Humans eat food in plastic utensils and pollute environment by burning too. The unsafe high levels of the plastic waste are not good for both environment and living organisms, either humans or animals. To cut down the usage of plastic products to the safe levels, some safe alternatives are needed. Edible cutlery can serve as a sustainable alternative to the plastic cutlery because it’s biodegradable and edible. Edible cutlery is made of safe-to-eat ingredients like wheat, rice and sorghum. Edible cutlery may be vegan-friendly with natural spices and flavors. It requires no additives or preservatives. Environmental pollution in Pakistan can be controlled significantly by promoting the use of edible cutlery like the other countries. Edible cutlery is the best solution to spread awareness regarding environmental issues due to plastic use. This review entailed the benefits of edible cutlery usage for the betterment of human health","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117090842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessment of Water, Sanitation, and Hygiene Practices among School Going Children","authors":"Warda Kiran, A. John, T. Faridi, Mishal John","doi":"10.54692/lgujls.2022.0602216","DOIUrl":"https://doi.org/10.54692/lgujls.2022.0602216","url":null,"abstract":"In rural areas, many important hygiene practices are unavailable causing gastrointestinal and respiratory infections in school-going children. The objective of this study was to assess water, sanitation, and hygiene practices among school-going children in rural areas of Gujrat, Pakistan. A cross-sectional study was conducted among school-going children in different schools in Gujrat, Pakistan. The data was collected in 4 months from December, 2021 to March, 2022 with a total number of 1000 participants. Data collection was carried out using a close-ended structured questionnaire among different school-going children. It contained a total of 14 questions about hygiene practices in children. Data was entered and analyzed on SPSS version 26 and frequencies and percentages were mentioned as their responses in the form of tables and pie charts. The results of the current study comprised responses of 1000 boys and girls from different schools. The responses showed that 601(60.1%) children did not wash their hands before and after meals in school while, 568(56.8%) children did not flush before and after the toilet, 578(57.8%) did not wash their genital organs, 995(99.5%) drank tap water in schools, and 646(64.6%) drank 5-10 glasses of water per day. It was concluded by the study that most of school-going children did not follow hygienic practices and there is need to highlight the issues","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124565509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. A. Khattak, Shahid Nawaz, A. Fatima, Anayat Ur Rahman, Tauseef Ahmad, Shahbaz Ahmad
{"title":"Is Brucellosis an emerging zoonotic infection in District Nowshera Pakistan? [A novel investigation involving descriptive analysis of laboratory reports (2019-2021)]","authors":"M. A. Khattak, Shahid Nawaz, A. Fatima, Anayat Ur Rahman, Tauseef Ahmad, Shahbaz Ahmad","doi":"10.54692/lgujls.2022.0602215","DOIUrl":"https://doi.org/10.54692/lgujls.2022.0602215","url":null,"abstract":"A novel cross-sectional study was planned to determine the significant frequency, risk factors and clinical parameters of Brucellosis in people living in Nowshera, Pakistan from March 2019 to March 2021. A non-probability convenient sampling was performed targeting 617 individuals who were previously diagnosed with consistent fever and cough, abortion and infertility, musculoskeletal pain, headache and depression, and elevated serum Lactate dehydrogenase (LDH), elevated C-reactive Protein (CRP), and elevated Erythrocytes sedimentation rate (ESR). A closed-end questionnaire was filled out to gather information regarding different socio- demographics, assumed potential risk factors and clinical parameters of the participants. The serum samples of all participants were tested for IgG ELISA using (NovaLisa, GmbH, Germany). Descriptive statistics and statistical significance (null hypothesis) were analyzed via SPSS. 21. The entire frequency of human brucellosis found in our study was 14.1%. The highly significant sociodemographic factors were gender (P=0.00001), residency (P=0.040), and financial status (P=0.007), whereas the significant risk factors were animal exposure (P=0.002) and Brucella awareness (P=0.003). While the highly significant clinical complications were abortion and infertility (P=0.00004), elevated LDH (P=0.0005), elevated CRP (P=0.00003) and ESR (P=0.0002). The present seroprevalence of brucellosis in Nowshera is an alarming and serious public health concern. It is highly recommended to publicly provide a massive hygienic awareness and diagnostic services to explore the updates regarding the epidemiology of Brucellosis in the considered area for reasonable eradication","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"91 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131884695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rubee Anwar, S. Hussain, Muhammad Amin Abid, Muhammad Ahmad, M. Javed, M. Pervaiz
{"title":"Pharmacological and Phytochemical Potential of Aleo Barbadensis (A Comprehensive Review)","authors":"Rubee Anwar, S. Hussain, Muhammad Amin Abid, Muhammad Ahmad, M. Javed, M. Pervaiz","doi":"10.54692/lgujls.2022.0602212","DOIUrl":"https://doi.org/10.54692/lgujls.2022.0602212","url":null,"abstract":"Aloe vera contains 99-99.5% water; the remaining (0.5- 1.0%) solid material is comprised of minerals, water & fat-soluble vitamins, amino acids, phenolic compounds, simple and complex polysaccharides and organic acids. Aloe gel contains different elements like B, Be, Al, P, Si, Ca, Mg, Na and Fe. On the basis of dry-weight, the rind and pulp contain only a minor fraction of proteins and lipids. Gel polysaccharides of Aloe vera are comprised of linear chains of glucose and mannose molecules. Aloe vera consists of more than 200 different biological and medicinal active ingredients which are highly beneficial for human health. The plant demonstrates the antibacterial and antifungal, anti-helmenthic, purgative, anti-inflammatory, aphrodisiac, antispectic, anti-oxiant and laxative properties and is also used to treat skin infections, skin cancer, minor cuts and even sunburns; it is also effective against piles, jaundice, cough, dyspnea and asthma. The plant is commonly used at local level in Pakistan, for the treatment of numerous human diseases. Aloe vera also finds very important applications in cosmetics, dermatological industry, beauty products, sun block lotions, skin smoothening moisturizers, skin tonics against pimples and also have the ability to transport healthy substances through the skin and removal of dead skin cells. The present study was aimed to review the phytochemical composition and medicinal potential of Aloe barbadensis (Aloe vera) which is a very famous plant all over the world.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123518262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Saeed, N. Zahra, Neelam Shahadat, K. Shahzadi, Syed Hussain Imam Abdi, Q. Syed
{"title":"Extraction of poppy seed (Papaver somniferum L.) oil and its antioxidant activity by DPPH Assay","authors":"M. Saeed, N. Zahra, Neelam Shahadat, K. Shahzadi, Syed Hussain Imam Abdi, Q. Syed","doi":"10.54692/lgujls.2022.0602211","DOIUrl":"https://doi.org/10.54692/lgujls.2022.0602211","url":null,"abstract":"The huge mainstream of plants, especially agricultural raw materials possesses extractable oils that may have some commercial value. Poppy seeds have high nutritional value and are used as a source of food and cooking oil. Its oil is rich in polyunsaturated fatty acids. It is well known that polyunsaturated fatty acids not only provide basic nutrients for the body, but their absorption into the body are very important for the prevention of cardiovascular disease and many inflammatory diseases. In this study, poppy seed oil was extracted with a yield of 32.50 ± 1.80% and free radical scavenging activity was evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method. Antioxidant activity was found to be in the range of 22.28±1.40-58.32±3.40% at concentrations of 20-100 µl. It was concluded in this study that poppy seeds were found as an effective source of natural antioxidants and can be used to substitute synthetic antioxidants","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"282 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127553789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Zahra, M. Saeed, K. Shahzad, Shamma Firdous, I. Ahmad, M. Ashraf, Syed Hussain Imam Abidi, Q. Syed
{"title":"DPPH Assay and Reducing Power Activity ofWater Extract of (Mentha longifolia) Mint","authors":"N. Zahra, M. Saeed, K. Shahzad, Shamma Firdous, I. Ahmad, M. Ashraf, Syed Hussain Imam Abidi, Q. Syed","doi":"10.54692/lgujls.2022.0601198","DOIUrl":"https://doi.org/10.54692/lgujls.2022.0601198","url":null,"abstract":"Antioxidative properties of plants may be associated with oxidative stress defence in different human diseases. Oxidative stress and free radicals can be neutralized by antioxidants which are of great significance in preventing the expansion of these diseases. Many studies have investigated the toxic effect of synthetic antioxidants, thus to avoid these toxic effects new antioxidants of natural origin have been deliberated in recent years. Mint (Mentha longifolia) is extensively used as medicine, spice, food and flavouring. In the current work, an aqueous extract of mint leaves was determined using the DPPH assay and reducing power activity. Strapping and sound antioxidant effects were observed in 2,2-diphenyl-1- picrylhydrazyl radicals at concentrations of 0.1-0.5 mg/ml, ranging from 20.32 ± 0.35 % to 65.75 ± 1.5 %, while standard antioxidant BHT possess % Inhibition (DPPH) 30.41 ± 0.65 % to 83.50 ± 2.3 % at same concentration. A similar effect was found in the reducing power assay, which exhibited absorbance of M. longifolia water extract ranging from 0.1501 ± 0.010 to 0.5845 ± 0.042 and BHT exhibited 0.3221 ± 0.026 to 0.8197 ± 0.124 at 700 nm. The conclusion recommends that M. longifolia has confirmed vital benefits due to high concentration of antioxidants and has vast impending for claim in the preparation of useful food entities.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131054122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessment of Aflatoxins production and its various Control strategies: A Review","authors":"S. M. Hassan, N. Afzal","doi":"10.54692/lgujls.2022.0601201","DOIUrl":"https://doi.org/10.54692/lgujls.2022.0601201","url":null,"abstract":"Mycotoxins specifically the aflatoxins contaminate about 25% of the agricultural products in the world. They effect greatly the growth rate, feed intake, and feed conversion ratio. There is a great economical loss to farmers due to loss of food values and effecting human as well as animal health. The different preventive measures are in use to control fungal contamination such as chemicals (acids, bases, and salts), HSCAS ,zeolite polymers, activated charcoal and yeast with variable findings. Fraction of yeast sludge has proved to minimize the adverse effects of aflatoxicosis due to the presence of manno-oligosaccharides in its cell wall. The present review highlights the remedial measures particularly yeast sludge to control aflatoxicosis.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131605432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Hussain, M. Faizan, M. Javed, Muhammad Ahmad, S. Nadeem, F. Feroz, A. Rauf, L. Rasool, Aliya Ibrar, M. Fatima
{"title":"Effectiveness of Face Masks and Respiratory Aid Devices for Prophylaxis against COVID-19","authors":"S. Hussain, M. Faizan, M. Javed, Muhammad Ahmad, S. Nadeem, F. Feroz, A. Rauf, L. Rasool, Aliya Ibrar, M. Fatima","doi":"10.54692/lgujls.2022.0601197","DOIUrl":"https://doi.org/10.54692/lgujls.2022.0601197","url":null,"abstract":"The COVID-19 epidemic was spread rapidly around the world in 2019, and has greatly affected peoples general interaction, their work habits, dailylives and businesses. A personnel protection against the spread of COVID-19 is not only beneficial but is critically important especially for those working in medical fields. Current studies were performed to review the use and benefits of face mask and respiratory aid devices in order to control the spread of COVID-19. The respiratory aids devices (RADs) may be recommended as anti-COVID-19 masks rather than surgical masks. However, RADs having P and R series filters do not prevent the infiltration of coronavirus due to its smaller size as compared to the filtration capacity of filters. The N99 and N100 filters cause a difficulty inbreathing; it is difficult to wear such sort of respirators for a longer time. N95 respirators are recommended by medical professionals as they provide lesser(almost half) respiratory resistance. However, wearing of face masks is also involved some risks and side effects which may include physiological and disturbing effects, difficulty in breathing, affecting the volume and quality of sound, decrease of innate immunity, cardiopulmonary overload and increase in contamination chances due to development of humid habitat inside the mask.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121518891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}