Lahore Garrison University Journal of Life Sciences最新文献

Diversity of Human Skin Microbiota in Healthcare Workers of South Punjab, Pakistan during COVID-19 2019冠状病毒病期间巴基斯坦南旁遮普省卫生保健工作者皮肤微生物群的多样性

Lahore Garrison University Journal of Life Sciences Pub Date : 2023-06-26 DOI: 10.54692/lgujls.2023.0702265

Anosh Arshad Chaudhary, Iqra Arooj, A. Elahi, S. Ijaz, H. Yasmeen

{"title":"Diversity of Human Skin Microbiota in Healthcare Workers of South Punjab, Pakistan during COVID-19","authors":"Anosh Arshad Chaudhary, Iqra Arooj, A. Elahi, S. Ijaz, H. Yasmeen","doi":"10.54692/lgujls.2023.0702265","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0702265","url":null,"abstract":"Human skin microflora plays important role in the functioning of skin and is modulated by several intrinsic and extrinsic factors including hygiene practices. During Covid-19 pandemic, focus has been particularly directed towards improving hygiene. We explored the skin microflora of healthcare workers in local population of Multan, Pakistan. A total of 44 samples of skin were collected from healthy workers along with the administration of questionnaire regarding hygiene practices. After isolation, bacteria were characterized by morphology, staining andbiochemical tests. Majority of the workers was 20-30 years old females. Most of them reported to wear gloves during practice, wash hands 8-10 times a day, use sanitizer 8-10 times a week and took bath 12-15 times a month. Isolated microflora (n=110) included Staphylococcus aureus (62%), Escherichia coli (16%), Pseudomonas spp. (9%), Proteus spp. (5%), Enterobacter spp. (5%) and Klebsiella spp. (3%). Presence of pathogens, although in small numbers, emphasizes the necessity of disseminating knowledge regarding adoption and maintenance of hygienic practices, specifically among healthcare workers.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133480492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Visual Perception in Children with Autism Spectrum Disorder 自闭症谱系障碍儿童视觉知觉的评估

Lahore Garrison University Journal of Life Sciences Pub Date : 2023-06-26 DOI: 10.54692/lgujls.2023.0702256

Hafiza Iqra Javaid, Shamsa Kanwal, Muhammad Ahmed, Sadia Akbar, Amna Rashid, Saba Yaqoob, T. Faridi

引用次数: 0

Deviations in Thyroid and its Regulatory Hormone Profile in Workers Exposed to Welding Fumes 接触焊接烟雾的工人甲状腺及其调节激素谱的偏差

Lahore Garrison University Journal of Life Sciences Pub Date : 2023-06-26 DOI: 10.54692/lgujls.2023.0702254

Salman Farooq, Z. W. Aziz, M. A. Iqbal, Kaleem Maqsood, N. Roohi

{"title":"Deviations in Thyroid and its Regulatory Hormone Profile in Workers Exposed to Welding Fumes","authors":"Salman Farooq, Z. W. Aziz, M. A. Iqbal, Kaleem Maqsood, N. Roohi","doi":"10.54692/lgujls.2023.0702254","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0702254","url":null,"abstract":"This study evaluates health risks manifested by the exposure of welding fumes to the labourers working in welding environments. Welding fumes, are the complexes of fluorides, silicates and metal oxides, cause burns, eye damage, hormonal imbalance, organ damage and cataracts. In order to check the changes caused by fumes on thyroid gland, a study was planned to evaluate the variations in thyroid regulatory hormone levels in workers occupationally exposed to welding fumes. For this purpose, blood sampling of the welders (n=24) having exposure to welding fumes was done from different sites in city Lahore, whereas, blood samples of healthy controls (n=24) were collected from University of the Punjab, Lahore. Thyroid (T3 and T4) and its regulatory hormone (TSH) assessment was executed by ELISA. Independent student “t” test at confidence interval of 95% and P < 0.05 was applied. A non-significant decrease of both hormones i.e. T3 and T4 was found in the subjects under study as compared to controls. Levels of TSH in blood serum of workers showed a non-significant increase than healthy controls. Elevated TSH and reduced T3 and T4, although, statistically non-significant, predict chances of hypothyroidism due to chronic exposure to welding fumes. Welders are, therefore, recommended to adopt prophylactic measures and safer techniques in order to avoid direct hazardous exposure to welding fumes","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129461168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Silico Comparative Metagenomic Analysis of Microbial Communities of Chromium Contaminated Sites 铬污染场地微生物群落的比较宏基因组分析

Lahore Garrison University Journal of Life Sciences Pub Date : 2023-06-26 DOI: 10.54692/lgujls.2023.0702264

Rabia Sadiq, Nazia Kanwal, Yasir Rehman

{"title":"In Silico Comparative Metagenomic Analysis of Microbial Communities of Chromium Contaminated Sites","authors":"Rabia Sadiq, Nazia Kanwal, Yasir Rehman","doi":"10.54692/lgujls.2023.0702264","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0702264","url":null,"abstract":"Chromium is one of the highly toxic and carcinogenic heavy metals. Due to increased anthropogenic activities, high concentration of chromium is found in many areas. Many microorganisms have the ability to detoxify chromium. Metagenomics allow us to comprehensively study microbial communities present at different sites without culturing them. The objective of this study was to analyze the abundance of microbial groups in different environments contaminated with chromium. For this purpose, chromium contaminated soil, anaerobic sludge and reactor samples were chosen. 16S rRNA data of these samples was retrieved from NCBI SRA database. The sequences were analyzed by Mothur software accessed via Galaxy server, and were classified using SILVA database. Venn diagram, phylogenetic tree, heatmap, relative abundance graphs and Krona pie charts were generated. Statistical analysis was also performed in the form of AMOVA and HOMOVA tests. According to results of our study, Proteobacteria, Leucobacter, Actinomycetales, Actinobacteria, Arthrobacter, Rhizobiales, Sphingomonas, Bradyrizobium and Nucardioidaceae were present in all the samples. Firmicutes, Planctomycetes, Verrucomicrobia and Bacteroidetes were more abundant in chromium contaminated samples as compared to control samples. The results were also found to be statistically significant. The above-mentioned bacteria can be targeted and studied to discover their roles in bioremediation of chromium contaminated sites.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121416257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of Aloe vera in human health especially against COVID-19 芦荟对人体健康尤其是对抗COVID-19的功效

Lahore Garrison University Journal of Life Sciences Pub Date : 2023-06-26 DOI: 10.54692/lgujls.2023.0702263

Fareeha Iqbal, Ambreen Ahmed

引用次数: 0

Brief analysis of Therapeutic Approaches of Type 1 Diabetes Mellitus along with Diagnosis and Screening Methods 浅析1型糖尿病的治疗途径及诊断和筛查方法

Lahore Garrison University Journal of Life Sciences Pub Date : 2023-06-26 DOI: 10.54692/lgujls.2023.0702266

Rida Tanveer, Iqra Fasih, Syed Ahmad, Samreen Riaz

{"title":"Brief analysis of Therapeutic Approaches of Type 1 Diabetes Mellitus along with Diagnosis and Screening Methods","authors":"Rida Tanveer, Iqra Fasih, Syed Ahmad, Samreen Riaz","doi":"10.54692/lgujls.2023.0702266","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0702266","url":null,"abstract":"Diabetes is an endocrine system disease which is characterized by abnormal elevated glucose levels. Type I Diabetes Mellitus (T1DM) is an autoimmune disorder with early onset, whereas Type 2 Diabetes Mellitus (T2DM) is non-autoimmune form with late onset. Small and large artery complications are the two main categories of diabetes mellitus long term complications. Overproduction of superoxide by the mitochondrial electron transport chain (ETC), leading to oxidative stress, occurs because of pathogenic effects of hyperglycemia. New vessels are fragile and hyper permeable in case of retinopathy in T1DM. T1DM is known to be occurred by beta cell destruction which leads to hyperglycemia and insulin scantiness. In phase 3 T1DM is normally diagnosed, the stage at which the disorder has led to life threatening condition known as diabetic ketoacidosis. To minimize the possibility of serious complication it is necessary to diagnose autoimmunity which is present during first years of life through early screening or by using diagnostic tools. Measuring fasting blood glucose or standard OGTT’s are performed for screening of phase 2 T1DM in the persons which have 1 or more autoantibodies targeting β-cell. The management of type 1 diabetes mellitus is necessary to encourage healthy lifestyle and to control glycaemia conditions in order to avoid severe complication. Pharmacological approaches are the most widely used method for the treatment of T1DM including injectable insulin and sodium glucose cotransporter 2 (SGLT2) inhibitors, Gene therapy and stem cellbased therapies. These are supposed to help in providing life-time freedom from T1DM but there is still a room for debate in this regard.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"5093 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132068647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and Frequency Distribution of HLA-A, HLA-B and HLA-C Alleles in the Punjab, Pakistan 巴基斯坦旁遮普省HLA-A、HLA-B和HLA-C等位基因的患病率和频率分布

Lahore Garrison University Journal of Life Sciences Pub Date : 2023-06-26 DOI: 10.54692/lgujls.2023.0702259

Saba Sajjad, Imran Tipu, Muhammad Ali, Muhammad Irfan Fareed, Rana Muhammad Kamran Shabbir, Faiza Samrez, Fareeha Ahmed, Wasiq Ikram, Rubina Dad, Mureed Hussain, Rana Muhammad Mateen, Rukhsana Parveen, Muhammad Sohail Afzal

引用次数: 0

Prevalence and Management of Endoparasitic Worm load in Ostriches of Different Captive Conditions Housed in Punjab, Pakistan 巴基斯坦旁遮普省不同圈养条件下鸵鸟体内寄生虫负荷的流行及管理

Lahore Garrison University Journal of Life Sciences Pub Date : 2023-06-26 DOI: 10.54692/lgujls.2023.0702260

Bushra Nisar Khan, Iqra Islam, Nisar Ahmed, Muhammad Oneeb, Muhammad Azhar, Kiran Saleem, Muhammad Rizwan Khan

{"title":"Prevalence and Management of Endoparasitic Worm load in Ostriches of Different Captive Conditions Housed in Punjab, Pakistan","authors":"Bushra Nisar Khan, Iqra Islam, Nisar Ahmed, Muhammad Oneeb, Muhammad Azhar, Kiran Saleem, Muhammad Rizwan Khan","doi":"10.54692/lgujls.2023.0702260","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0702260","url":null,"abstract":"Ostrich farming has attained a status of a fast-growing agribusiness in the livestock industry due to the wide range of associated benefits attached to it. This study was designed to assess the prevalence of endoparasites in this economically important bird as well as their treatment to provide better guidelines for successful ostrich farming. The study involved 385 feacal samples of ostriches taken from 55 commercial farms and captive sites Punjab during the period of January 2020 to December 2020. The relevant data and samples were collected from 15 districts of Punjab. Feacal samples were tested against gastrointestinal worm load by using floatation and sedimentation techniques. Ostriches of 11 commercial farms and Lahore Zoo, Jallo Wildlife Park Lahore, UVAS Ostrich farm Pattoki and Bahawalnagar Wildlife Park were found to harbor protozoan parasites such as Eimeria spp. Balantidium coli, and Amoeboid cyst. It was noteworthy that the use of herbal dewormers showed much better results than chemical anthelmintics. Similarly, those wildlife parks where ostriches were given a natural environment showed negative results for endoparasites. At some farms combination of chemical and herbal anthelmintics was also used. The absence of a reliable nutritional management system caused the mortality of ostriches at 40 farms due to gastric problems and choking. The mortality rate at the age of 2-4 months was 73% while mortality of adult birds from 1-7 years was 27%. Among selected farms, 50% of ostriches were facing lameness, leg deformities, and retarded growth due to improper space, a congested environment, and poor feeding systems. Lastly, more research is needed to make this agribusiness flourished.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126319081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Aflatoxins Exposure on Human Health and its Management Strategies 黄曲霉毒素暴露对人体健康的影响及其管理策略

Lahore Garrison University Journal of Life Sciences Pub Date : 2023-06-26 DOI: 10.54692/lgujls.2023.0702258

N. Zahra, Muhammad Hassan Raza, Faheem Hafeez, M. Saeed, S. A. Khan, A. Saeed, K. Shahzad

引用次数: 0

In Silico Analysis of MARS1 Gene to Elucidate Low- Frequency Variants Associated with Interstitial Lung and Liver Disease 用计算机分析MARS1基因阐明与间质性肺和肝脏疾病相关的低频变异

Lahore Garrison University Journal of Life Sciences Pub Date : 2023-03-08 DOI: 10.54692/lgujls.2023.0701240

Aamna Syed, R. Mateen, Ayman Naeem, Zainab Asif Mirza, Muhammad Usman Ghani, M. Hussain

{"title":"In Silico Analysis of MARS1 Gene to Elucidate Low- Frequency Variants Associated with Interstitial Lung and Liver Disease","authors":"Aamna Syed, R. Mateen, Ayman Naeem, Zainab Asif Mirza, Muhammad Usman Ghani, M. Hussain","doi":"10.54692/lgujls.2023.0701240","DOIUrl":"https://doi.org/10.54692/lgujls.2023.0701240","url":null,"abstract":"Mutation in MARS1 gene is linked to the development of Interstitial lung and liver disease. The current study aimed in silico analysis to predict the most harmful missense and spliced variants of MARS1 that damage the functionality of Methionyl-tRNA synthetase 1 (MARS 1), catalyses the ligation of methionine to tRNA and is essential forprotein biosynthesis. A total of 492 variants were retrieved from the gnomAD database and analysed by CADD, 308 missense variants with PHRED score ≥ 20 were further analysed by CAPICE, META-SNP and CONDEL.85 SNPs detected with deleterious impact on protein structure by screening nsSNPs. Moreover, in-silico stability analysis was done by different tools like DynaMut, DUET, i-Stable2.0 and YASARA. MARS1 protein structure obtained from RCSB PDB (PDB ID: 5GL7) and UCSF Chimera was used for its visualisation. NetSurf-2.0 obtained the analysis of protein functioning by position of residue in the structure. Our results showed that the structure of proteins was significantly deleterious and protein motif and function were changed, we proceeded to use the PROSITE database to forecast the posttranslation modification sites and four significant nsSNPs with protein structure change effects. Splice analysis was conducted by SPiCE, Human Splice Finder. It concludes in silico analysis, genes can determine likely pathogenic variation for further in vitro experimental study.","PeriodicalId":148827,"journal":{"name":"Lahore Garrison University Journal of Life Sciences","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115510777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0