JOR SpinePub Date : 2026-05-15eCollection Date: 2026-06-01DOI: 10.1002/jsp2.70186
Claire L Samanna, Christopher Neason, Scott D Tagliaferri, Ulrike H Mitchell, Michelle Caldwell, Ali Imran, Hana Rae Nez, Daniel L Belavý, Clint T Miller, David Scott, Niamh L Mundell, Paul Buntine, Patrick J Owen
{"title":"Lifetime Physical Loading and Magnetic Resonance-Derived Intervertebral Disc Health in Adults With Chronic Low Back Pain: A Cross-Sectional Study.","authors":"Claire L Samanna, Christopher Neason, Scott D Tagliaferri, Ulrike H Mitchell, Michelle Caldwell, Ali Imran, Hana Rae Nez, Daniel L Belavý, Clint T Miller, David Scott, Niamh L Mundell, Paul Buntine, Patrick J Owen","doi":"10.1002/jsp2.70186","DOIUrl":"10.1002/jsp2.70186","url":null,"abstract":"<p><strong>Background: </strong>The intervertebral disc (IVD) is a mechanosensitive structure influenced by physical loading (physical activity/sport/exercise); however, the optimal type and parameters of physical loading for IVD health remain unclear. The Bone Physical Activity Questionnaire is validated to predict bone mineral density from lifetime exposure to physical loading; therefore, we explored the association between lifetime physical loading and IVD health.</p><p><strong>Methods: </strong>This cross-sectional study recruited 40 individuals with chronic low back pain. Outcomes were magnetic resonance imaging-derived IVD measures reported as both averages and across individual spinal levels T11/T12-L5/S1, including T2 (ms, measure of hydration), height-to-vertebral-body ratio, nucleus-to-annulus signal intensity ratio, volume (cm<sup>3</sup>), and Pfirrmann grade (0-5 points). Multiple linear regression examined lifetime physical loading and IVD health while controlling for significant covariates (age, body mass index, current and past occupational sitting, and physical labour).</p><p><strong>Results: </strong>Lifetime bone-related physical loading was not associated with IVD health across average spine values, except nucleus-to-annulus signal intensity ratio, which was negatively associated (<i>β</i> [95% CI]: -0.00 [-0.01, -0.00], <i>p</i> = 0.030). At L5/S1, IVD T2 (-0.30 [-0.56, -0.05], <i>p</i> = 0.022) and nucleus-to-annulus signal intensity (-0.02 [-0.03, -0.01], <i>p</i> = 0.007) were negatively associated, and Pfirrmann grade (0.02 [0.01, 0.03], <i>p</i> = 0.006) was positively associated with physical loading.</p><p><strong>Conclusions: </strong>Physical activity loading necessary for improved IVD health appears to differ from loading previously reported for optimal bone health. Specifically, lifetime activity levels considered beneficial for bone were associated with less favourable L5/S1 IVD characteristics, highlighting distinct loading requirements across these tissues. Future studies could develop a questionnaire that captures optimal physical loading for IVD health, such as moderate physical loading.</p>","PeriodicalId":14876,"journal":{"name":"JOR Spine","volume":"9 ","pages":"e70186"},"PeriodicalIF":3.9,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13179145/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147963711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
JOR SpinePub Date : 2026-04-22eCollection Date: 2026-06-01DOI: 10.1002/jsp2.70182
Irina Heggli, Alon Lai, Denise Iliff, Marco D Burkhard, Niklas Koehne, Harsev Singh, Jonathan J Huang, Alan C Seifert, Damien Laudier, Noah Bonnheim, Levon Rodriguez, Timothy D Jacobsen, James C Iatridis
{"title":"Intradiscal <i>Cutibacterium acnes</i> Sustains Modic Type 1-Like Lesions Over Time in a Rat Lumbar Endplate Injury Model.","authors":"Irina Heggli, Alon Lai, Denise Iliff, Marco D Burkhard, Niklas Koehne, Harsev Singh, Jonathan J Huang, Alan C Seifert, Damien Laudier, Noah Bonnheim, Levon Rodriguez, Timothy D Jacobsen, James C Iatridis","doi":"10.1002/jsp2.70182","DOIUrl":"10.1002/jsp2.70182","url":null,"abstract":"<p><strong>Background: </strong>Modic changes (MC) are painful vertebral bone marrow lesions. Three interconvertible types represent different pathology stages with MC1 being the most painful, MC2 less painful, and MC3 asymptomatic. Bacterial (<i>Cutibacterium acnes</i> (<i>C. acnes</i>)-mediated) and non-bacterial etiologies are both suggested to induce MC1, but it remains unclear whether MC conversion is etiology-specific. This has important implications for patient management and the development of MC therapies.</p><p><strong>Aims: </strong>To assess MC-subtype lesion prevalence and pain-like behavior over time in an etiology-specific MC rat model.</p><p><strong>Methods: </strong>Four-to-five-month-old Sprague-Dawley rats underwent sham (<i>n</i> = 12) surgery or endplate injury followed by an intradiscal injection of 2.5 μL of either TNF-α (1000 ng/mL PBS) (<i>n</i> = 19) or <i>C. acnes</i> (3.2 × 10<sup>8</sup> CFU/mL in PBS) (<i>n</i> = 18) to mimic aspects of bacterial versus non-bacterial MC etiologies. MC-subtype prevalence was evaluated by magnetic resonance imaging at 1, 8, and 14 weeks post-injury. Disc degeneration, bone marrow lesion neutrophil elastase and CD19 immunoreactivity, spinal cord sensitization, and pain-like behavior (von Frey) were assessed, and contributors to pain-like behavior were identified (random forest).</p><p><strong>Results: </strong><i>C. acnes</i> injection caused higher MC1-like lesion prevalence than TNF-α and Sham at all-time points and higher spinal cord substance P expression at 14 weeks post-injury. MC2-like lesions increased over time in TNF-α-injected discs and were more prevalent at 14 weeks post-injury than <i>C. acnes</i>. <i>C. acnes</i> injection resulted in bone marrow lesions with higher neutrophil elastase- and CD19 immunoreactivity. MC1-like lesions contributed strongest to pain-like behavior, although von Frey did not differ between groups.</p><p><strong>Conclusion: </strong>Intradiscal <i>C. acnes</i> injection sustained MC1-like lesions in an etiology-specific lumbar MC rat model, suggesting that patients with bacterial MC etiology may be more likely to persist in painful MC1.</p>","PeriodicalId":14876,"journal":{"name":"JOR Spine","volume":"9 ","pages":"e70182"},"PeriodicalIF":3.9,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13102498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147772123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detrimental Effect of Low-Magnitude High-Frequency Vibration in an Ex Vivo Model of Intervertebral Disc Degeneration Despite Estrogen Treatment.","authors":"Jan Ulrich Jansen, Felizitas Figel, Franziska Widmayer, Morten Vogt, Maria Ahrens, Hans-Joachim Wilke, Anita Ignatius, Melanie Haffner-Luntzer, Cornelia Neidlinger-Wilke, Graciosa Quelhas Teixeira","doi":"10.1002/jsp2.70183","DOIUrl":"10.1002/jsp2.70183","url":null,"abstract":"<p><strong>Introduction: </strong>Postmenopausal females are at increased risk of intervertebral disc (IVD) degeneration due to estrogen decline. While both estrogen supplementation and low-magnitude high-frequency vibration (LMHFV) are proposed as therapies for osteoporosis, their effects and potential interactions on IVD degeneration remain unclear. Therefore, the aim of this work was to investigate the individual and combined effects of 17β-oestradiol (E2) and LMHFV in a papain (PP)-induced ex vivo model of IVD degeneration.</p><p><strong>Methods: </strong>Bovine IVDs were degraded with PP (65 U/mL) and subsequently treated with E2 (10<sup>-7</sup> M), LMHFV (45 Hz, 0.3 g, 20 min/day), or both; untreated discs served as controls. Gene expression was analyzed on Day 8. On Day 21, cell viability, DNA content, extracellular matrix components (glycosaminoglycan [GAG], collagen), inflammatory (interleukin-6 [IL-6]) and catabolic (matrix metalloproteinase-3 [MMP-3]) markers, and annular stiffness and strength were assessed.</p><p><strong>Results: </strong>PP digestion reduced cell viability and decreased GAG content, while increasing IL-6 and MMP-3 production. Treatment with E2 partially restored <i>ESR1</i> expression but did not affect cell viability or matrix metabolism. LMHFV (PP + LMHFV group) significantly increased DNA and GAG content compared with PP alone, whereas the combined treatment (PP + E2 + LMHFV) further elevated IL-6 and MMP-3 production. Annular stiffness was higher following PP treatment but was normalized by all subsequent treatments.</p><p><strong>Conclusion: </strong>In a severely degenerated IVD model, LMHFV promoted catabolic and inflammatory responses, and E2 treatment was insufficient to counteract these effects. These findings suggest that LMHFV, commonly used to support bone health in osteoporotic patients, may pose risks to IVD integrity in advanced degeneration.</p>","PeriodicalId":14876,"journal":{"name":"JOR Spine","volume":"9 ","pages":"e70183"},"PeriodicalIF":3.9,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13101075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147772083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
