Japanese journal of toxicology and environmental health最新文献

{"title":"Enzymatic Determination of Aspartame in Carbonated Beverages","authors":"K. Boki, K. Matsuda, H. Asada, A. Hirose, Katsunori Kondo","doi":"10.1248/JHS1956.39.72","DOIUrl":"https://doi.org/10.1248/JHS1956.39.72","url":null,"abstract":"The determination of aspartame in carbonated beverages was investigated by enzymatic method. Conditions for determining aspartame were as follows : time of hydrolysis by 6 N HCl, 5 h at 110°C ; concentration and pH of 2-amino-2-methyl-1, 3-propanediol-HCl buffer, 200 mM and 7.5 ; reaction temperature, 37°C ; reaction time, 5 h ; GOT, 0.655 IU/ml ; MDH, 0.234 IU/ml ; α-ketoglutarate ; 0.615 mM ; concentration of NADH, 0.1 mM. The method gave high recoveries (93.9-102.1%) of aspartame to amount added (0.058-0.339 mg/ml) into carbonated beverages. The minimum limit of determination was 6μg/ml.","PeriodicalId":14851,"journal":{"name":"Japanese journal of toxicology and environmental health","volume":"7 1","pages":"72-75"},"PeriodicalIF":0.0,"publicationDate":"1993-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87656549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Changes of Organic Compounds in Chlorinated Water. XIX. Production of Alkylpolychlorinated Phenoxyphenols (Predioxins) by Aqueous Chlorination of Alkylphenols","authors":"S. Onodera, Mariko Takahashi, Shizuo Suzuki","doi":"10.1248/JHS1956.39.20","DOIUrl":"https://doi.org/10.1248/JHS1956.39.20","url":null,"abstract":"Aqueous alkylphenol solutions were treated with chlorine at 20°C under various experimental conditions. Changes in the compositions of the chlorination products in water were determined by gas chromatographic (GC) and GC-mass spectrometric (GC-MS) analyses of diethyl ether extracts. Alkylphenols were shown to produce a variety of chlorinated compounds, including alkylpolychlorinated phenoxyphenols (predioxins), having one to five chlorine atoms, as by-products in chlorine-treated water. Production of some of these predioxins is dependent on the number of equivalents of chlorine per mol of compound and on the reaction pH. These results suggest that chlorine treatment of water contaminated with alkylphenols leads to the production of predioxins, which are precursors of the highly toxic chlorinated dibenzo-p-dioxins.","PeriodicalId":14851,"journal":{"name":"Japanese journal of toxicology and environmental health","volume":"5 1","pages":"20-28"},"PeriodicalIF":0.0,"publicationDate":"1993-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85831613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent progress of HPLC analysis for drugs of abuse","authors":"Y. Nakahara","doi":"10.1248/JHS1956.39.5_369","DOIUrl":"https://doi.org/10.1248/JHS1956.39.5_369","url":null,"abstract":"Recent progress of high performance liquid chromatography (HPLC) for drugs of abuse was reviewed on over 100 papers. First, papers concerning screening analyses for drugs of abuse using HPLC were reviewed and discussed about their usefulness. Secondly, the progress of new analytical methods for high-sensitive detection of amphetamines, morphine, codeine, 6-acetylmorphine, cannabinoids, cocaine and others were reviewed by dividing into 1. fluorometric, 2. electrochemical, and 3. chemiluminescence detections. Thirdly, new studies on the chiral assay for amphetamines using chiral derivatization or chiral column were reviewed. Furthermore, the application to discrimination between use of over-the-counter drugs and illegal amphetamines was exemplified. Fourthly, the utility of a photodiode array detector and a mass detector for the confirmation of drugs was shown by citing several papers. Moreover, it was discussed about automation of the HPLC analysis using the column switching method, capillary electrophoresis and supercritical fluid chromatography applied for the analysis of drugs of abuse.","PeriodicalId":14851,"journal":{"name":"Japanese journal of toxicology and environmental health","volume":"23 1","pages":"369-380"},"PeriodicalIF":0.0,"publicationDate":"1993-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90891298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rapid determination of metallothionein in animal tissues by the Cd-Chelex method","authors":"M. Kiyozumi, Y. Sakaguchi, E. Shiozuka, Y. Matsuda, Etsuko Yamamoto, S. Kojima","doi":"10.1248/JHS1956.39.4_345","DOIUrl":"https://doi.org/10.1248/JHS1956.39.4_345","url":null,"abstract":"Determination of metallothionein (MT) by the Cd-Chelex method was investigated using dry Chelex, and the Cd-Chelex and Cd-hem methods were compared for the estimation of MT from the brain, heart, lung, liver, kidney, spleen, and testis of rats injected with CdCl2. There was in relatively good agreement between these two methods on the determination of the tissues MT containing Zn and Cd.","PeriodicalId":14851,"journal":{"name":"Japanese journal of toxicology and environmental health","volume":"69 1","pages":"345-349"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79156405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"食品添加物(酸化防止剤)のウサギ血小板機能に及ぼす影響","authors":"Hiroyasu Yamazaki, Takako Yamaguchi, A. Yamauchi, Y. Kakiuchi","doi":"10.1248/jhs1956.39.5_453","DOIUrl":"https://doi.org/10.1248/jhs1956.39.5_453","url":null,"abstract":"","PeriodicalId":14851,"journal":{"name":"Japanese journal of toxicology and environmental health","volume":"41 1","pages":"453-458"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81521139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studies on the Biological Effects of Lanthanum-Effects of Repeated Oral Administration Tests in Rats.","authors":"Y. Ogawa","doi":"10.1248/JHS1956.38.545","DOIUrl":"https://doi.org/10.1248/JHS1956.38.545","url":null,"abstract":"In order to elucidate the series of the biological effect of the rare earth elements, a 28-d repeated oral administration test was conducted in Slc : Wistar rats of each sex by gavage at doses of 0, 40, 200 or 1000 mg/kg/d of YCl3·6H2O with the pair feeding group. Additionally two more groups (0 mg/kg, 1000 mg/kg) of both sexes were maintained for a 14-d recovery period after the 28-d administration. Body weight and food consumption were measured, and hematological, serum-biochemical and histopathological examination were performed. The concentrations of yttrium and essential elements in organs were determined by ICP-MS or ICP-AES. These overall data on yttrium were compared with our previous study on lanthanum performed by the same protocol. The results were as follows. (1) The number of eosinophilic leucocytes increased in rats of both sexes dose-dependently. (2) At a dose of 1000 mg/kg, hyperkeratosis in the forestomach of both sexes, eosinophilic leucocyte infiltration in the submucosa of the stomach of both sexes, erosion and dilatation of gastric gland of the glandular stomach in males, and swelling of the glandular stomach epithelium in females were found. These results suggest that yttrium is irritant to the stomach mucosa. (3) At doses higher than 200 mg/kg, a significant decrease of the serum cholinesterase activity was observed in females. (4) At the 1000 mg/kg lanthanum-dosed rats of our previous study, the serum transaminase activity increased in both sexes. On the other hand, there were no changes between control and yttrium-dosed rats. (5) Yttrium was accumulated in the kidney, femur, liver and spleen, in a dose-dependent manner. (6) The highest accumulation of yttrium was found in the kidney. According to the previous lanthanum study, the highest accumulation of lanthanum was observed in the liver. (7) The total amount of yttrium acumulation in rats was about 50 times as much as that of lanthamum. (8) The sex-dependent differences were observed in terms of the irritating reaction of the stomach mucosa, the reduction of the serum cholinesterase activity and the accumulation of yttrium in the kidney. (9) These results show that yttrium could be absorbed through the digestive tract. (10) Iron concentrations in the liver, kidney and spleen, and barium and strontium concentrations in the femur were dose-dependently decreased in both sexes of yttrium-treated rats. Both yttrium and lanthanum are classified as rare earth elements and the present study revealed that the biological effects of yttrium were very similar to those of lanthanum except the accumulating patterns and volumes. On the other hand, in contrast to the fact that lanthanum was hepatotoxic compound, yttrium had no effect on liver, which seems to be due to the difference of their accumulating patterns.","PeriodicalId":14851,"journal":{"name":"Japanese journal of toxicology and environmental health","volume":"20 1","pages":"545-553"},"PeriodicalIF":0.0,"publicationDate":"1992-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82818952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Corticosteroids and Anabolic Agents in Health Food by High Performance Liquid Chromatography","authors":"H. Terada, Katsuhiko Yamamoto, M. Miyabe","doi":"10.1248/JHS1956.38.537","DOIUrl":"https://doi.org/10.1248/JHS1956.38.537","url":null,"abstract":"","PeriodicalId":14851,"journal":{"name":"Japanese journal of toxicology and environmental health","volume":"236 1","pages":"537-544"},"PeriodicalIF":0.0,"publicationDate":"1992-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75013600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"染毛剤原料m-phenylenediamineの過酸化水素処理時に生ずる変異原性の硫酸鉄(II), フェノール化合物並びに金属キレートによる阻害","authors":"Tetsushi Watanabe, Masanori Kusumoto, Seiji Hatatani, Sachiko Matsuoka, Tomoko Toyoda, Teruhisa Hirayama","doi":"10.1248/JHS1956.38.529","DOIUrl":"https://doi.org/10.1248/JHS1956.38.529","url":null,"abstract":"","PeriodicalId":14851,"journal":{"name":"Japanese journal of toxicology and environmental health","volume":"88 1","pages":"529-536"},"PeriodicalIF":0.0,"publicationDate":"1992-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82652169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Lysozyme in Various Foodstuffs by High Performance Liquid Chromatography with Fluorescence Detector","authors":"T. Shibata, S. Tsuji, Kazuhiro Kobayashi, Y. Asai, Tsuneo Honda, K. Noda, M. Iwaida, E. Mochizuki, Osamu Sugahara, Yoshio N. Ito","doi":"10.1248/JHS1956.38.437","DOIUrl":"https://doi.org/10.1248/JHS1956.38.437","url":null,"abstract":"","PeriodicalId":14851,"journal":{"name":"Japanese journal of toxicology and environmental health","volume":"27 1","pages":"437-442"},"PeriodicalIF":0.0,"publicationDate":"1992-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83487737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Pathways of Morphine and Their Relation to Hepatotoxicity","authors":"K. Nagamatsu","doi":"10.1248/JHS1956.38.305","DOIUrl":"https://doi.org/10.1248/JHS1956.38.305","url":null,"abstract":"The review on our works mainly consists of metabolic activation of morphine related to in vivo and in vitro hepatotoxicity. Especially, the biotransformation of morphinone-glutathione conjugate from morphine, and the role of the reactive metabolite of morphine (morphinone) has been discussed in detail.","PeriodicalId":14851,"journal":{"name":"Japanese journal of toxicology and environmental health","volume":"155 1","pages":"305-312"},"PeriodicalIF":0.0,"publicationDate":"1992-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77603065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}